Restriction map for
pF1KB8113
(Circular) (Six-base) MAPSORT of: pF1KB8113.seq Check: 6051 from: 1 to: 4253
pF1KB8113 4253 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 387 895 387
Size: 508 3745
AccI GT'mk_AC
Cuts at: 203 1291 203
Size: 1088 3165
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 675 2078 2510 3257 3525 675
Size: 1403 432 747 268 1403
Fragments arranged by size:
1403 1403 747 432 268
AflIII A'CryG_T
Cuts at: 198 1605 2709 3686 198
Size: 1407 1104 977 765
Fragments arranged by size:
1407 1104 977 765
AgeI A'CCGG_T
Cuts at: 2641 2641
Size: 4253
AleI CACnn'nnGTG
Cuts at: 973 973
Size: 4253
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 722 754 722
Size: 32 4221
AlwNI CAG_nnn'CTG
Cuts at: 1050 1558 3125 1050
Size: 508 1567 2178
Fragments arranged by size:
2178 1567 508
ApaLI G'TGCA_C
Cuts at: 3023 3023
Size: 4253
ApoI r'AATT_y
Cuts at: 1257 3606 3862 1257
Size: 2349 256 1648
Fragments arranged by size:
2349 1648 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 1273 1273
Size: 4253
BamHI G'GATC_C
Cuts at: 956 1278 956
Size: 322 3931
BanI G'GyrC_C
Cuts at: 339 1269 1683 1931 1966 339
Size: 930 414 248 35 2626
Fragments arranged by size:
2626 930 414 248 35
BanII G_rGCy'C
Cuts at: 434 992 1153 1267 2297 434
Size: 558 161 114 1030 2390
Fragments arranged by size:
2390 1030 558 161 114
BbeI G_GCGC'C
Cuts at: 1687 1935 1687
Size: 248 4005
BbsI GAAGACnn'nnnn_
Cuts at: 191 900 191
Size: 709 3544
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4219
BciVI GTATCCnnnnn_n'
Cuts at: 2151 2912 4253 2151
Size: 761 1341 2151
Fragments arranged by size:
2151 1341 761
BclI T'GATC_A
Cuts at: 1773 1773
Size: 4253
BglI GCCn_nnn'nGGC
Cuts at: 559 559
Size: 4253
BglII A'GATC_T
Cuts at: 219 1768 3805 219
Size: 1549 2037 667
Fragments arranged by size:
2037 1549 667
BlpI GC'TnA_GC
Cuts at: 1365 1365
Size: 4253
Bme1580I G_kGCm'C
Cuts at: 342 1878 1971 3027 342
Size: 1536 93 1056 1568
Fragments arranged by size:
1568 1536 1056 93
BmgBI CAC'GTC
Cuts at: 199 199
Size: 4253
BmrI ACTGGGnnnn_n'
Cuts at: 482 636 1465 1546 1881 4025 482
Size: 154 829 81 335 2144 710
Fragments arranged by size:
2144 829 710 335 154 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 97 540 865 1006 97
Size: 443 325 141 3344
Fragments arranged by size:
3344 443 325 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1429 2308 2800 3098 481
Size: 948 879 492 298 1636
Fragments arranged by size:
1636 948 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3957 3957
Size: 4253
BsaAI yAC'GTr
Cuts at: 285 1606 285
Size: 1321 2932
BsaBI GATnn'nnATC
Cuts at: 6 1767 3804 6
Size: 1761 2037 455
Fragments arranged by size:
2037 1761 455
BsaHI Gr'CG_yC
Cuts at: 1684 1932 2634 4130 1684
Size: 248 702 1496 1807
Fragments arranged by size:
1807 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1442 1963 2641 2915 3062 3473 1442
Size: 521 678 274 147 411 2222
Fragments arranged by size:
2222 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 484 1177 1196 484
Size: 693 19 3541
Fragments arranged by size:
3541 693 19
BseYI C'CCAG_C
Cuts at: 799 1174 1678 2436 3013 3509 799
Size: 375 504 758 577 496 1543
Fragments arranged by size:
1543 758 577 504 496 375
BsiEI CG_ry'CG
Cuts at: 104 1795 1841 3049 104
Size: 1691 46 1208 1308
Fragments arranged by size:
1691 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 631 992 1153 1267 2045 2235 3027 631
Size: 361 161 114 778 190 792 1857
Fragments arranged by size:
1857 792 778 361 190 161 114
Bsp1286I G_dGCh'C
Cuts at: 342 434 631 992 1153 1267 1878 1971 2045
Size: 92 197 361 161 114 611 93 74
Cuts at: 2045 2235 2297 3027 342
Size: 190 62 730 1568
Fragments arranged by size:
1568 730 611 361 197 190 161 114
93 92 74 62
BspEI T'CCGG_A
Cuts at: 3473 3473
Size: 4253
BspHI T'CATG_A
Cuts at: 3429 3429
Size: 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 387 848 895 1303 1517 1819 2200 387
Size: 461 47 408 214 302 381 2440
Fragments arranged by size:
2440 461 408 381 302 214 47
BsrBI CCG'CTC
Cuts at: 50 428 2545 2599 4083 4248 50
Size: 378 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 378 165 55 54
BsrDI GCAATG_nn'
Cuts at: 548 695 963 2165 548
Size: 147 268 1202 2636
Fragments arranged by size:
2636 1202 268 147
BsrFI r'CCGG_y
Cuts at: 482 553 1193 2251 2432 2641 482
Size: 71 640 1058 181 209 2094
Fragments arranged by size:
2094 1058 640 209 181 71
BssSI C'ACGA_G
Cuts at: 2524 2882 2524
Size: 358 3895
BstAPI GCAn_nnn'nTGC
Cuts at: 1630 1630
Size: 4253
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstXI CCAn_nnnn'nTGG
Cuts at: 399 399
Size: 4253
BstYI r'GATC_y
Cuts at: 1 219 956 1278 1745 1768 2103 2349 3361
Size: 218 737 322 467 23 335 246 1012
Cuts at: 3361 3447 3459 3805 1
Size: 86 12 346 449
Fragments arranged by size:
1012 737 467 449 346 335 322 246
218 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2203 2384 2547 2203
Size: 181 163 3909
Fragments arranged by size:
3909 181 163
BtsI GCAGTG_nn'
Cuts at: 1134 1496 1134
Size: 362 3891
DraI TTT'AAA
Cuts at: 1252 1252
Size: 4253
DraIII CAC_nnn'GTG
Cuts at: 747 747
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 1959 2817 1959
Size: 858 3395
EaeI y'GGCC_r
Cuts at: 438 560 749 1209 1838 2012 2403 2430 3775
Size: 122 189 460 629 174 391 27 1345
Cuts at: 3775 438
Size: 916
Fragments arranged by size:
1345 916 629 460 391 189 174 122
27
EagI C'GGCC_G
Cuts at: 1838 1838
Size: 4253
EarI CTCTTCn'nnn_
Cuts at: 256 694 834 2276 2486 256
Size: 438 140 1442 210 2023
Fragments arranged by size:
2023 1442 438 210 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 2771 2917 4063 2461
Size: 310 146 1146 2651
Fragments arranged by size:
2651 1146 310 146
EcoICRI GAG'CTC
Cuts at: 990 1151 1265 990
Size: 161 114 3978
Fragments arranged by size:
3978 161 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 97 540 675 865 1006 2078 2510 3257 3525
Size: 443 135 190 141 1072 432 747 268
Cuts at: 3525 97
Size: 825
Fragments arranged by size:
1072 825 747 443 432 268 190 141
135
EcoO109I rG'GnC_Cy
Cuts at: 1392 1392
Size: 4253
EcoRI G'AATT_C
Cuts at: 1257 1257
Size: 4253
EcoRV GAT'ATC
Cuts at: 616 616
Size: 4253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 427 2272 427
Size: 1845 2408
FspI TGC'GCA
Cuts at: 1081 2034 1081
Size: 953 3300
HaeII r_GCGC'y
Cuts at: 1687 1935 2957 3936 1687
Size: 248 1022 979 2004
Fragments arranged by size:
2004 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 926 958 2095 2125 2127 2157 400
Size: 32 494 32 1137 30 2 30 2496
Fragments arranged by size:
2496 1137 494 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1292 1292
Size: 4253
KasI G'GCGC_C
Cuts at: 1683 1931 1683
Size: 248 4005
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2924 3108 2924
Size: 184 4069
MscI TGG'CCA
Cuts at: 751 1211 2014 751
Size: 460 803 2990
Fragments arranged by size:
2990 803 460
MslI CAynn'nnrTG
Cuts at: 973 2242 2369 973
Size: 1269 127 2857
Fragments arranged by size:
2857 1269 127
MspA1I CmG'CkG
Cuts at: 518 1047 1174 1364 1678 2038 3051 3296 518
Size: 529 127 190 314 360 1013 245 1475
Fragments arranged by size:
1475 1013 529 360 314 245 190 127
NaeI GCC'GGC
Cuts at: 555 1195 2434 555
Size: 640 1239 2374
Fragments arranged by size:
2374 1239 640
NarI GG'CG_CC
Cuts at: 1684 1932 1684
Size: 248 4005
NgoMIV G'CCGG_C
Cuts at: 553 1193 2432 553
Size: 640 1239 2374
Fragments arranged by size:
2374 1239 640
NspI r_CATG'y
Cuts at: 1306 1806 2713 1306
Size: 500 907 2846
Fragments arranged by size:
2846 907 500
PciI A'CATG_T
Cuts at: 2709 2709
Size: 4253
PflMI CCAn_nnn'nTGG
Cuts at: 747 747
Size: 4253
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 442 474 3392 3424 442
Size: 32 2918 32 1271
Fragments arranged by size:
2918 1271 32 32
PstI C_TGCA'G
Cuts at: 1173 1300 1985 1173
Size: 127 685 3441
Fragments arranged by size:
3441 685 127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 518 1047 1174 1678 2038 518
Size: 529 127 504 360 2733
Fragments arranged by size:
2733 529 504 360 127
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 992 1153 1267 992
Size: 161 114 3978
Fragments arranged by size:
3978 161 114
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 256 694 2276 2486 256
Size: 438 1582 210 2023
Fragments arranged by size:
2023 1582 438 210
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SfcI C'TryA_G
Cuts at: 33 1042 1169 1296 1981 2974 3165 33
Size: 1009 127 127 685 993 191 1121
Fragments arranged by size:
1121 1009 993 685 191 127 127
SfoI GGC'GCC
Cuts at: 1685 1933 1685
Size: 248 4005
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 1275 1275
Size: 4253
SmlI C'TyrA_G
Cuts at: 496 1408 2323 2815 3077 496
Size: 912 915 492 262 1672
Fragments arranged by size:
1672 915 912 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
StyI C'CwwG_G
Cuts at: 356 1387 1741 356
Size: 1031 354 2868
Fragments arranged by size:
2868 1031 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 203 1836 1969 2428 2637 203
Size: 1633 133 459 209 1819
Fragments arranged by size:
1819 1633 459 209 133
TatI w'GTAC_w
Cuts at: 3768 3768
Size: 4253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 133 165 3770 3802 133
Size: 32 3605 32 584
Fragments arranged by size:
3605 584 32 32
Tth111I GACn'n_nGTC
Cuts at: 2050 2050
Size: 4253
XbaI T'CTAG_A
Cuts at: 180 1284 180
Size: 1104 3149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 354 1748 354
Size: 1394 2859
XmaI C'CCGG_G
Cuts at: 1273 1273
Size: 4253
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI