Restriction map for
pF1KB8115
(Circular) (Six-base) MAPSORT of: pF1KB8115.seq Check: 8492 from: 1 to: 4313
pF1KB8115 4313 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1351 1351
Size: 4313
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 280 610 2138 2570 3317 3585 232
Size: 48 330 1528 432 747 268 960
Fragments arranged by size:
1528 960 747 432 330 268 48
AflIII A'CryG_T
Cuts at: 1665 2769 3746 1665
Size: 1104 977 2232
Fragments arranged by size:
2232 1104 977
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 1618 3185 1618
Size: 1567 2746
ApaLI G'TGCA_C
Cuts at: 3083 3083
Size: 4313
ApoI r'AATT_y
Cuts at: 120 430 510 760 1026 1087 1133 1317 3666
Size: 310 80 250 266 61 46 184 2349
Cuts at: 3666 3922 120
Size: 256 511
Fragments arranged by size:
2349 511 310 266 256 250 184 80
61 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 1333 1333
Size: 4313
AvrII C'CTAG_G
Cuts at: 687 687
Size: 4313
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 1329 1743 1991 2026 1329
Size: 414 248 35 3616
Fragments arranged by size:
3616 414 248 35
BanII G_rGCy'C
Cuts at: 286 920 1327 2357 286
Size: 634 407 1030 2242
Fragments arranged by size:
2242 1030 634 407
BbeI G_GCGC'C
Cuts at: 1747 1995 1747
Size: 248 4065
BbsI GAAGACnn'nnnn_
Cuts at: 238 1181 238
Size: 943 3370
BciVI GTATCCnnnnn_n'
Cuts at: 2211 2972 4313 2211
Size: 761 1341 2211
Fragments arranged by size:
2211 1341 761
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglII A'GATC_T
Cuts at: 852 1828 3865 852
Size: 976 2037 1300
Fragments arranged by size:
2037 1300 976
BlpI GC'TnA_GC
Cuts at: 704 1425 704
Size: 721 3592
Bme1580I G_kGCm'C
Cuts at: 1938 2031 3087 1938
Size: 93 1056 3164
Fragments arranged by size:
3164 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1525 1606 1941 4085 1525
Size: 81 335 2144 1753
Fragments arranged by size:
2144 1753 335 81
BmtI G_CTAG'C
Cuts at: 994 994
Size: 4313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 786 1489 2368 2860 3158 490
Size: 296 703 879 492 298 1645
Fragments arranged by size:
1645 879 703 492 298 296
BsaI GGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 4313
BsaAI yAC'GTr
Cuts at: 1666 1666
Size: 4313
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 1744 1992 2694 4190 1744
Size: 248 702 1496 1867
Fragments arranged by size:
1867 1496 702 248
BsaWI w'CCGG_w
Cuts at: 217 1502 2023 2701 2975 3122 3533 217
Size: 1285 521 678 274 147 411 997
Fragments arranged by size:
1285 997 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 839 869 839
Size: 30 4283
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 176 928 176
Size: 752 3561
BseYI C'CCAG_C
Cuts at: 1101 1738 2496 3073 3569 1101
Size: 637 758 577 496 1845
Fragments arranged by size:
1845 758 637 577 496
BsiEI CG_ry'CG
Cuts at: 104 1855 1901 3109 104
Size: 1751 46 1208 1308
Fragments arranged by size:
1751 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 920 1327 2105 2295 3087 920
Size: 407 778 190 792 2146
Fragments arranged by size:
2146 792 778 407 190
Bsp1286I G_dGCh'C
Cuts at: 286 920 1327 1938 2031 2105 2295 2357 3087
Size: 634 407 611 93 74 190 62 730
Cuts at: 3087 286
Size: 1512
Fragments arranged by size:
1512 730 634 611 407 190 93 74
62
BspEI T'CCGG_A
Cuts at: 217 3533 217
Size: 3316 997
BspHI T'CATG_A
Cuts at: 375 3489 375
Size: 3114 1199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1159 1363 1577 1879 2260 1159
Size: 204 214 302 381 3212
Fragments arranged by size:
3212 381 302 214 204
BsrBI CCG'CTC
Cuts at: 50 2605 2659 4143 4308 50
Size: 2555 54 1484 165 55
Fragments arranged by size:
2555 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 332 940 2225 332
Size: 608 1285 2420
Fragments arranged by size:
2420 1285 608
BsrFI r'CCGG_y
Cuts at: 2311 2492 2701 2311
Size: 181 209 3923
Fragments arranged by size:
3923 209 181
BsrGI T'GTAC_A
Cuts at: 564 966 564
Size: 402 3911
BssSI C'ACGA_G
Cuts at: 2584 2942 2584
Size: 358 3955
BstAPI GCAn_nnn'nTGC
Cuts at: 1283 1690 1283
Size: 407 3906
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstYI r'GATC_y
Cuts at: 1 852 1140 1338 1805 1828 2163 2409 3421
Size: 851 288 198 467 23 335 246 1012
Cuts at: 3421 3507 3519 3865 1
Size: 86 12 346 449
Fragments arranged by size:
1012 851 467 449 346 335 288 246
198 86 23 12
BtgI C'CryG_G
Cuts at: 256 256
Size: 4313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2263 2444 2607 2263
Size: 181 163 3969
Fragments arranged by size:
3969 181 163
BtsI GCAGTG_nn'
Cuts at: 1281 1556 1281
Size: 275 4038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1188 1223 1188
Size: 35 4278
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 2019 2877 2019
Size: 858 3455
EaeI y'GGCC_r
Cuts at: 1898 2072 2463 2490 3835 1898
Size: 174 391 27 1345 2376
Fragments arranged by size:
2376 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1898 1898
Size: 4313
EarI CTCTTCn'nnn_
Cuts at: 936 2336 2546 936
Size: 1400 210 2703
Fragments arranged by size:
2703 1400 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2521 2831 2977 4123 2521
Size: 310 146 1146 2711
Fragments arranged by size:
2711 1146 310 146
EcoICRI GAG'CTC
Cuts at: 918 1325 918
Size: 407 3906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 280 610 2138 2570 3317 3585 232
Size: 48 330 1528 432 747 268 960
Fragments arranged by size:
1528 960 747 432 330 268 48
EcoO109I rG'GnC_Cy
Cuts at: 1452 1452
Size: 4313
EcoRI G'AATT_C
Cuts at: 430 510 1087 1317 430
Size: 80 577 230 3426
Fragments arranged by size:
3426 577 230 80
EcoRV GAT'ATC
Cuts at: 381 381
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 562 827 2332 562
Size: 265 1505 2543
Fragments arranged by size:
2543 1505 265
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 1747 1995 3017 3996 1747
Size: 248 1022 979 2064
Fragments arranged by size:
2064 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2155 2185 2187 2217 2155
Size: 30 2 30 4251
Fragments arranged by size:
4251 30 30 2
HincII GTy'rAC
Cuts at: 794 1352 794
Size: 558 3755
HpaI GTT'AAC
Cuts at: 794 794
Size: 4313
KasI G'GCGC_C
Cuts at: 1743 1991 1743
Size: 248 4065
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MfeI C'AATT_G
Cuts at: 1269 1269
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2984 3168 2984
Size: 184 4129
MscI TGG'CCA
Cuts at: 2074 2074
Size: 4313
MslI CAynn'nnrTG
Cuts at: 2302 2429 2302
Size: 127 4186
MspA1I CmG'CkG
Cuts at: 1105 1424 1738 2098 3111 3356 1105
Size: 319 314 360 1013 245 2062
Fragments arranged by size:
2062 1013 360 319 314 245
NaeI GCC'GGC
Cuts at: 2494 2494
Size: 4313
NarI GG'CG_CC
Cuts at: 1744 1992 1744
Size: 248 4065
NcoI C'CATG_G
Cuts at: 256 256
Size: 4313
NgoMIV G'CCGG_C
Cuts at: 2492 2492
Size: 4313
NheI G'CTAG_C
Cuts at: 990 990
Size: 4313
NspI r_CATG'y
Cuts at: 1366 1866 2773 1366
Size: 500 907 2906
Fragments arranged by size:
2906 907 500
PciI A'CATG_T
Cuts at: 2769 2769
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PstI C_TGCA'G
Cuts at: 1096 1360 2045 1096
Size: 264 685 3364
Fragments arranged by size:
3364 685 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 1105 1738 2098 1105
Size: 633 360 3320
Fragments arranged by size:
3320 633 360
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 920 1327 920
Size: 407 3906
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 529 1092 1356 2041 3034 3225 33
Size: 496 563 264 685 993 191 1121
Fragments arranged by size:
1121 993 685 563 496 264 191
SfoI GGC'GCC
Cuts at: 1745 1993 1745
Size: 248 4065
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 505 801 1468 2383 2875 3137 505
Size: 296 667 915 492 262 1681
Fragments arranged by size:
1681 915 667 492 296 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
SspI AAT'ATT
Cuts at: 350 1224 350
Size: 874 3439
StyI C'CwwG_G
Cuts at: 256 435 687 1447 1801 256
Size: 179 252 760 354 2768
Fragments arranged by size:
2768 760 354 252 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1896 2029 2488 2697 1896
Size: 133 459 209 3512
Fragments arranged by size:
3512 459 209 133
TatI w'GTAC_w
Cuts at: 564 966 3828 564
Size: 402 2862 1049
Fragments arranged by size:
2862 1049 402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4281
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 229 1344 229
Size: 1115 3198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1808 1808
Size: 4313
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
XmnI GAAnn'nnTTC
Cuts at: 875 1124 875
Size: 249 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmgBI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI
HindIII NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI