Restriction map for
pF1KB8116
(Circular) (Six-base) MAPSORT of: pF1KB8116.seq Check: 9245 from: 1 to: 3872
pF1KB8116 3872 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 235 910 235
Size: 675 3197
Acc65I G'GTAC_C
Cuts at: 888 888
Size: 3872
AclI AA'CG_TT
Cuts at: 3712 3712
Size: 3872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 1697 2129 2876 3144 719
Size: 978 432 747 268 1447
Fragments arranged by size:
1447 978 747 432 268
AflIII A'CryG_T
Cuts at: 1224 2328 3305 1224
Size: 1104 977 1791
Fragments arranged by size:
1791 1104 977
AgeI A'CCGG_T
Cuts at: 2260 2260
Size: 3872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 226 226
Size: 3872
AlwNI CAG_nnn'CTG
Cuts at: 642 757 1177 2744 642
Size: 115 420 1567 1770
Fragments arranged by size:
1770 1567 420 115
ApaI G_GGCC'C
Cuts at: 656 656
Size: 3872
ApaLI G'TGCA_C
Cuts at: 2642 2642
Size: 3872
ApoI r'AATT_y
Cuts at: 876 3225 3481 876
Size: 2349 256 1267
Fragments arranged by size:
2349 1267 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
AvaI C'yCGr_G
Cuts at: 892 892
Size: 3872
BamHI G'GATC_C
Cuts at: 897 897
Size: 3872
BanI G'GyrC_C
Cuts at: 170 888 1302 1550 1585 170
Size: 718 414 248 35 2457
Fragments arranged by size:
2457 718 414 248 35
BanII G_rGCy'C
Cuts at: 231 656 886 1916 231
Size: 425 230 1030 2187
Fragments arranged by size:
2187 1030 425 230
BbeI G_GCGC'C
Cuts at: 1306 1554 1306
Size: 248 3624
BbsI GAAGACnn'nnnn_
Cuts at: 759 759
Size: 3872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3838
BciVI GTATCCnnnnn_n'
Cuts at: 1770 2531 3872 1770
Size: 761 1341 1770
Fragments arranged by size:
1770 1341 761
BclI T'GATC_A
Cuts at: 1392 1392
Size: 3872
BglII A'GATC_T
Cuts at: 1387 3424 1387
Size: 2037 1835
BlpI GC'TnA_GC
Cuts at: 984 984
Size: 3872
Bme1580I G_kGCm'C
Cuts at: 656 1497 1590 2646 656
Size: 841 93 1056 1882
Fragments arranged by size:
1882 1056 841 93
BmrI ACTGGGnnnn_n'
Cuts at: 443 1084 1165 1500 3644 443
Size: 641 81 335 2144 671
Fragments arranged by size:
2144 671 641 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 3872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 808 829 319
Size: 489 21 3362
Fragments arranged by size:
3362 489 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1927 2419 2717 1048
Size: 879 492 298 2203
Fragments arranged by size:
2203 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3576 3576
Size: 3872
BsaAI yAC'GTr
Cuts at: 1225 1225
Size: 3872
BsaBI GATnn'nnATC
Cuts at: 6 1386 3423 6
Size: 1380 2037 455
Fragments arranged by size:
2037 1380 455
BsaHI Gr'CG_yC
Cuts at: 1303 1551 2253 3749 1303
Size: 248 702 1496 1426
Fragments arranged by size:
1496 1426 702 248
BsaWI w'CCGG_w
Cuts at: 1061 1582 2260 2534 2681 3092 1061
Size: 521 678 274 147 411 1841
Fragments arranged by size:
1841 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 466
Size: 3872
BseYI C'CCAG_C
Cuts at: 265 818 1297 2055 2632 3128 265
Size: 553 479 758 577 496 1009
Fragments arranged by size:
1009 758 577 553 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 658
Size: 3872
BsiEI CG_ry'CG
Cuts at: 104 1414 1460 2668 104
Size: 1310 46 1208 1308
Fragments arranged by size:
1310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 593 886 1664 1854 2646 593
Size: 293 778 190 792 1819
Fragments arranged by size:
1819 792 778 293 190
Bsp1286I G_dGCh'C
Cuts at: 231 593 656 886 1497 1590 1664 1854 1916
Size: 362 63 230 611 93 74 190 62
Cuts at: 1916 2646 231
Size: 730 1457
Fragments arranged by size:
1457 730 611 362 230 190 93 74
63 62
BspEI T'CCGG_A
Cuts at: 3092 3092
Size: 3872
BspHI T'CATG_A
Cuts at: 3048 3048
Size: 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 922 1136 1438 1819 347
Size: 575 214 302 381 2400
Fragments arranged by size:
2400 575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2164 2218 3702 3867 50
Size: 2114 54 1484 165 55
Fragments arranged by size:
2114 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 612 1784 612
Size: 1172 2700
BsrFI r'CCGG_y
Cuts at: 1870 2051 2260 1870
Size: 181 209 3482
Fragments arranged by size:
3482 209 181
BssSI C'ACGA_G
Cuts at: 2143 2501 2143
Size: 358 3514
BstAPI GCAn_nnn'nTGC
Cuts at: 1249 1249
Size: 3872
BstBI TT'CG_AA
Cuts at: 2233 2233
Size: 3872
BstYI r'GATC_y
Cuts at: 1 531 897 1364 1387 1722 1968 2980 3066
Size: 530 366 467 23 335 246 1012 86
Cuts at: 3066 3078 3424 1
Size: 12 346 449
Fragments arranged by size:
1012 530 467 449 366 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 461 461
Size: 3872
BtgI C'CryG_G
Cuts at: 222 222
Size: 3872
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1822 2003 2166 1822
Size: 181 163 3528
Fragments arranged by size:
3528 181 163
BtsI GCAGTG_nn'
Cuts at: 362 488 1115 362
Size: 126 627 3119
Fragments arranged by size:
3119 627 126
DraI TTT'AAA
Cuts at: 871 871
Size: 3872
DrdI GACnn_nn'nnGTC
Cuts at: 1578 2436 1578
Size: 858 3014
EaeI y'GGCC_r
Cuts at: 219 646 1457 1631 2022 2049 3394 219
Size: 427 811 174 391 27 1345 697
Fragments arranged by size:
1345 811 697 427 391 174 27
EagI C'GGCC_G
Cuts at: 1457 1457
Size: 3872
EarI CTCTTCn'nnn_
Cuts at: 806 863 1895 2105 806
Size: 57 1032 210 2573
Fragments arranged by size:
2573 1032 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2080 2390 2536 3682 2080
Size: 310 146 1146 2270
Fragments arranged by size:
2270 1146 310 146
EcoICRI GAG'CTC
Cuts at: 884 884
Size: 3872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 719 808 829 1697 2129 2876 3144 319
Size: 400 89 21 868 432 747 268 1047
Fragments arranged by size:
1047 868 747 432 400 268 89 21
EcoO109I rG'GnC_Cy
Cuts at: 465 622 652 653 1011 465
Size: 157 30 1 358 3326
Fragments arranged by size:
3326 358 157 30 1
EcoRI G'AATT_C
Cuts at: 876 876
Size: 3872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3872
FspI TGC'GCA
Cuts at: 1653 1653
Size: 3872
HaeII r_GCGC'y
Cuts at: 1306 1554 2576 3555 1306
Size: 248 1022 979 1623
Fragments arranged by size:
1623 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 528 560 1714 1744 1746 1776 528
Size: 32 1154 30 2 30 2624
Fragments arranged by size:
2624 1154 32 30 30 2
HincII GTy'rAC
Cuts at: 911 911
Size: 3872
KasI G'GCGC_C
Cuts at: 1302 1550 1302
Size: 248 3624
KpnI G_GTAC'C
Cuts at: 892 892
Size: 3872
MluI A'CGCG_T
Cuts at: 3305 3305
Size: 3872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 236 2543 2727 236
Size: 2307 184 1381
Fragments arranged by size:
2307 1381 184
MscI TGG'CCA
Cuts at: 221 648 1633 221
Size: 427 985 2460
Fragments arranged by size:
2460 985 427
MslI CAynn'nnrTG
Cuts at: 1861 1988 1861
Size: 127 3745
MspA1I CmG'CkG
Cuts at: 555 639 983 1297 1657 2670 2915 555
Size: 84 344 314 360 1013 245 1512
Fragments arranged by size:
1512 1013 360 344 314 245 84
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 3872
NarI GG'CG_CC
Cuts at: 1303 1551 1303
Size: 248 3624
NcoI C'CATG_G
Cuts at: 222 222
Size: 3872
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 3872
NspI r_CATG'y
Cuts at: 314 925 1425 2332 314
Size: 611 500 907 1854
Fragments arranged by size:
1854 907 611 500
PasI CC'CwG_GG
Cuts at: 145 145
Size: 3872
PciI A'CATG_T
Cuts at: 2328 2328
Size: 3872
PflMI CCAn_nnn'nTGG
Cuts at: 717 717
Size: 3872
PfoI T'CCnGG_A
Cuts at: 824 824
Size: 3872
PmeI GTTT'AAAC
Cuts at: 871 871
Size: 3872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3840
PpuMI rG'GwC_Cy
Cuts at: 622 622
Size: 3872
PspOMI G'GGCC_C
Cuts at: 652 652
Size: 3872
PstI C_TGCA'G
Cuts at: 193 428 776 919 1604 193
Size: 235 348 143 685 2461
Fragments arranged by size:
2461 685 348 235 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3872
PvuII CAG'CTG
Cuts at: 555 1297 1657 555
Size: 742 360 2770
Fragments arranged by size:
2770 742 360
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 3872
SacI G_AGCT'C
Cuts at: 886 886
Size: 3872
SalI G'TCGA_C
Cuts at: 909 909
Size: 3872
SapI GCTCTTCn'nnn_
Cuts at: 863 1895 2105 863
Size: 1032 210 2630
Fragments arranged by size:
2630 1032 210
SbfI CC_TGCA'GG
Cuts at: 919 919
Size: 3872
ScaI AGT'ACT
Cuts at: 3389 3389
Size: 3872
SfcI C'TryA_G
Cuts at: 33 189 424 772 915 1600 2593 2784 33
Size: 156 235 348 143 685 993 191 1121
Fragments arranged by size:
1121 993 685 348 235 191 156 143
SfoI GGC'GCC
Cuts at: 1304 1552 1304
Size: 248 3624
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
SmaI CCC'GGG
Cuts at: 894 894
Size: 3872
SmlI C'TyrA_G
Cuts at: 1027 1942 2434 2696 1027
Size: 915 492 262 2203
Fragments arranged by size:
2203 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3872
SphI G_CATG'C
Cuts at: 314 925 1425 314
Size: 611 500 2761
Fragments arranged by size:
2761 611 500
StyI C'CwwG_G
Cuts at: 222 1006 1360 222
Size: 784 354 2734
Fragments arranged by size:
2734 784 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1455 1588 2047 2256 1455
Size: 133 459 209 3071
Fragments arranged by size:
3071 459 209 133
TatI w'GTAC_w
Cuts at: 3387 3387
Size: 3872
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 3840
Tth111I GACn'n_nGTC
Cuts at: 1669 1669
Size: 3872
XbaI T'CTAG_A
Cuts at: 903 903
Size: 3872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1367 1367
Size: 3872
XmaI C'CCGG_G
Cuts at: 892 892
Size: 3872
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI