Restriction map for
pF1KB8119
(Circular) (Six-base) MAPSORT of: pF1KB8119.seq Check: 9067 from: 1 to: 3773
pF1KB8119 3773 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 371 371
Size: 3773
AccI GT'mk_AC
Cuts at: 163 811 163
Size: 648 3125
Acc65I G'GTAC_C
Cuts at: 789 789
Size: 3773
AclI AA'CG_TT
Cuts at: 3613 3613
Size: 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1598 2030 2777 3045 1598
Size: 432 747 268 2326
Fragments arranged by size:
2326 747 432 268
AflIII A'CryG_T
Cuts at: 1125 2229 3206 1125
Size: 1104 977 1692
Fragments arranged by size:
1692 1104 977
AgeI A'CCGG_T
Cuts at: 2161 2161
Size: 3773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 427 427
Size: 3773
AlwNI CAG_nnn'CTG
Cuts at: 571 1078 2645 571
Size: 507 1567 1699
Fragments arranged by size:
1699 1567 507
ApaI G_GGCC'C
Cuts at: 392 392
Size: 3773
ApaLI G'TGCA_C
Cuts at: 2543 2543
Size: 3773
ApoI r'AATT_y
Cuts at: 777 3126 3382 777
Size: 2349 256 1168
Fragments arranged by size:
2349 1168 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
AvaI C'yCGr_G
Cuts at: 393 793 393
Size: 400 3373
BamHI G'GATC_C
Cuts at: 798 798
Size: 3773
BanI G'GyrC_C
Cuts at: 789 1203 1451 1486 789
Size: 414 248 35 3076
Fragments arranged by size:
3076 414 248 35
BanII G_rGCy'C
Cuts at: 392 433 787 1817 392
Size: 41 354 1030 2348
Fragments arranged by size:
2348 1030 354 41
BbeI G_GCGC'C
Cuts at: 1207 1455 1207
Size: 248 3525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 596 630 596
Size: 34 3739
BciVI GTATCCnnnnn_n'
Cuts at: 1671 2432 3773 1671
Size: 761 1341 1671
Fragments arranged by size:
1671 1341 761
BclI T'GATC_A
Cuts at: 1293 1293
Size: 3773
BglI GCCn_nnn'nGGC
Cuts at: 157 157
Size: 3773
BglII A'GATC_T
Cuts at: 1288 3325 1288
Size: 2037 1736
BlpI GC'TnA_GC
Cuts at: 379 885 379
Size: 506 3267
Bme1580I G_kGCm'C
Cuts at: 392 416 1398 1491 2547 392
Size: 24 982 93 1056 1618
Fragments arranged by size:
1618 1056 982 93 24
BmgBI CAC'GTC
Cuts at: 300 300
Size: 3773
BmrI ACTGGGnnnn_n'
Cuts at: 234 318 985 1066 1401 3545 234
Size: 84 667 81 335 2144 462
Fragments arranged by size:
2144 667 462 335 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 3773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 358
Size: 3773
Bpu10I CC'TnA_GC
Cuts at: 427 427
Size: 3773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1828 2320 2618 949
Size: 879 492 298 2104
Fragments arranged by size:
2104 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3477 3477
Size: 3773
BsaAI yAC'GTr
Cuts at: 1126 1126
Size: 3773
BsaBI GATnn'nnATC
Cuts at: 6 1287 3324 6
Size: 1281 2037 455
Fragments arranged by size:
2037 1281 455
BsaHI Gr'CG_yC
Cuts at: 160 368 1204 1452 2154 3650 160
Size: 208 836 248 702 1496 283
Fragments arranged by size:
1496 836 702 283 248 208
BsaWI w'CCGG_w
Cuts at: 962 1483 2161 2435 2582 2993 962
Size: 521 678 274 147 411 1742
Fragments arranged by size:
1742 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 220 250 220
Size: 30 3743
BseYI C'CCAG_C
Cuts at: 1198 1956 2533 3029 1198
Size: 758 577 496 1942
Fragments arranged by size:
1942 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 210 354 1315 1361 2569 104
Size: 106 144 961 46 1208 1308
Fragments arranged by size:
1308 1208 961 144 106 46
BsiHKAI G_wGCw'C
Cuts at: 787 1565 1755 2547 787
Size: 778 190 792 2013
Fragments arranged by size:
2013 792 778 190
BsmI GAATG_Cn'
Cuts at: 612 612
Size: 3773
BsmBI CGTCTCn'nnnn_
Cuts at: 306 306
Size: 3773
Bsp1286I G_dGCh'C
Cuts at: 392 416 433 787 1398 1491 1565 1755 1817
Size: 24 17 354 611 93 74 190 62
Cuts at: 1817 2547 392
Size: 730 1618
Fragments arranged by size:
1618 730 611 354 190 93 74 62
24 17
BspEI T'CCGG_A
Cuts at: 2993 2993
Size: 3773
BspHI T'CATG_A
Cuts at: 2949 2949
Size: 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 464 823 1037 1339 1720 464
Size: 359 214 302 381 2517
Fragments arranged by size:
2517 381 359 302 214
BsrBI CCG'CTC
Cuts at: 50 2065 2119 3603 3768 50
Size: 2015 54 1484 165 55
Fragments arranged by size:
2015 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1685 1685
Size: 3773
BsrFI r'CCGG_y
Cuts at: 353 1771 1952 2161 353
Size: 1418 181 209 1965
Fragments arranged by size:
1965 1418 209 181
BssHII G'CGCG_C
Cuts at: 133 135 274 133
Size: 2 139 3632
Fragments arranged by size:
3632 139 2
BssSI C'ACGA_G
Cuts at: 181 2044 2402 181
Size: 1863 358 1552
Fragments arranged by size:
1863 1552 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1150 1150
Size: 3773
BstBI TT'CG_AA
Cuts at: 2134 2134
Size: 3773
BstXI CCAn_nnnn'nTGG
Cuts at: 492 492
Size: 3773
BstYI r'GATC_y
Cuts at: 1 798 1265 1288 1623 1869 2881 2967 2979
Size: 797 467 23 335 246 1012 86 12
Cuts at: 2979 3325 1
Size: 346 449
Fragments arranged by size:
1012 797 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 123 216 107
Size: 16 93 3664
Fragments arranged by size:
3664 93 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1723 1904 2067 1723
Size: 181 163 3429
Fragments arranged by size:
3429 181 163
BtsI GCAGTG_nn'
Cuts at: 139 1016 139
Size: 877 2896
DraI TTT'AAA
Cuts at: 772 772
Size: 3773
DrdI GACnn_nn'nnGTC
Cuts at: 1479 2337 1479
Size: 858 2915
EaeI y'GGCC_r
Cuts at: 110 207 320 351 1358 1532 1923 1950 3295
Size: 97 113 31 1007 174 391 27 1345
Cuts at: 3295 110
Size: 588
Fragments arranged by size:
1345 1007 588 391 174 113 97 31
27
EagI C'GGCC_G
Cuts at: 207 351 1358 207
Size: 144 1007 2622
Fragments arranged by size:
2622 1007 144
EarI CTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1981 2291 2437 3583 1981
Size: 310 146 1146 2171
Fragments arranged by size:
2171 1146 310 146
EcoICRI GAG'CTC
Cuts at: 785 785
Size: 3773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 1598 2030 2777 3045 358
Size: 1240 432 747 268 1086
Fragments arranged by size:
1240 1086 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 389 702 912 389
Size: 313 210 3250
Fragments arranged by size:
3250 313 210
EcoRI G'AATT_C
Cuts at: 777 777
Size: 3773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1792 1792
Size: 3773
FseI GG_CCGG'CC
Cuts at: 357 357
Size: 3773
FspI TGC'GCA
Cuts at: 1554 1554
Size: 3773
HaeII r_GCGC'y
Cuts at: 178 317 1207 1455 2477 3456 178
Size: 139 890 248 1022 979 495
Fragments arranged by size:
1022 979 890 495 248 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 358 390 713 745 1615 1645 1647
Size: 32 106 32 323 32 870 30 2
Cuts at: 1647 1677 220
Size: 30 2316
Fragments arranged by size:
2316 870 323 106 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 164 812 164
Size: 648 3125
KasI G'GCGC_C
Cuts at: 1203 1451 1203
Size: 248 3525
KpnI G_GTAC'C
Cuts at: 793 793
Size: 3773
MluI A'CGCG_T
Cuts at: 3206 3206
Size: 3773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 329 2444 2628 329
Size: 2115 184 1474
Fragments arranged by size:
2115 1474 184
MscI TGG'CCA
Cuts at: 322 1534 322
Size: 1212 2561
MslI CAynn'nnrTG
Cuts at: 1762 1889 1762
Size: 127 3646
MspA1I CmG'CkG
Cuts at: 125 407 435 884 1198 1558 2571 2816 125
Size: 282 28 449 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 449 360 314 282 245 28
NaeI GCC'GGC
Cuts at: 355 1954 355
Size: 1599 2174
NarI GG'CG_CC
Cuts at: 1204 1452 1204
Size: 248 3525
NcoI C'CATG_G
Cuts at: 107 107
Size: 3773
NdeI CA'TA_TG
Cuts at: 471 471
Size: 3773
NgoMIV G'CCGG_C
Cuts at: 353 1952 353
Size: 1599 2174
NspI r_CATG'y
Cuts at: 826 1326 2233 826
Size: 500 907 2366
Fragments arranged by size:
2366 907 500
PciI A'CATG_T
Cuts at: 2229 2229
Size: 3773
PflMI CCAn_nnn'nTGG
Cuts at: 698 698
Size: 3773
PfoI T'CCnGG_A
Cuts at: 704 704
Size: 3773
PmeI GTTT'AAAC
Cuts at: 772 772
Size: 3773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 3741
PpuMI rG'GwC_Cy
Cuts at: 702 702
Size: 3773
PshAI GACnn'nnGTC
Cuts at: 298 298
Size: 3773
PsiI TTA'TAA
Cuts at: 752 752
Size: 3773
PspOMI G'GGCC_C
Cuts at: 388 388
Size: 3773
PstI C_TGCA'G
Cuts at: 440 461 820 1505 440
Size: 21 359 685 2708
Fragments arranged by size:
2708 685 359 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3773
PvuII CAG'CTG
Cuts at: 1198 1558 1198
Size: 360 3413
RsrII CG'GwC_CG
Cuts at: 1968 1968
Size: 3773
SacI G_AGCT'C
Cuts at: 787 787
Size: 3773
SacII CC_GC'GG
Cuts at: 126 126
Size: 3773
SalI G'TCGA_C
Cuts at: 162 810 162
Size: 648 3125
SapI GCTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
SbfI CC_TGCA'GG
Cuts at: 461 820 461
Size: 359 3414
ScaI AGT'ACT
Cuts at: 3290 3290
Size: 3773
SfcI C'TryA_G
Cuts at: 33 436 457 816 1501 2494 2685 33
Size: 403 21 359 685 993 191 1121
Fragments arranged by size:
1121 993 685 403 359 191 21
SfoI GGC'GCC
Cuts at: 1205 1453 1205
Size: 248 3525
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
SmaI CCC'GGG
Cuts at: 395 795 395
Size: 400 3373
SmlI C'TyrA_G
Cuts at: 928 1843 2335 2597 928
Size: 915 492 262 2104
Fragments arranged by size:
2104 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3773
SphI G_CATG'C
Cuts at: 826 1326 826
Size: 500 3273
SspI AAT'ATT
Cuts at: 748 748
Size: 3773
StuI AGG'CCT
Cuts at: 235 235
Size: 3773
StyI C'CwwG_G
Cuts at: 107 578 907 1261 107
Size: 471 329 354 2619
Fragments arranged by size:
2619 471 354 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1356 1489 1948 2157 1356
Size: 133 459 209 2972
Fragments arranged by size:
2972 459 209 133
TatI w'GTAC_w
Cuts at: 3288 3288
Size: 3773
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 3741
Tth111I GACn'n_nGTC
Cuts at: 1570 1570
Size: 3773
XbaI T'CTAG_A
Cuts at: 804 804
Size: 3773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 333 1268 333
Size: 935 2838
XmaI C'CCGG_G
Cuts at: 393 793 393
Size: 400 3373
ZraI GAC'GTC
Cuts at: 369 369
Size: 3773
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI BseRI BsgI BsiWI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PmlI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI