Restriction map for
pF1KB8126
(Circular) (Six-base) MAPSORT of: pF1KB8126.seq Check: 7865 from: 1 to: 4772
pF1KB8126 4772 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1810 1810
Size: 4772
Acc65I G'GTAC_C
Cuts at: 1399 1788 1399
Size: 389 4383
AclI AA'CG_TT
Cuts at: 798 1334 4612 798
Size: 536 3278 958
Fragments arranged by size:
3278 958 536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 946 1540 2597 3029 3776 4044 389
Size: 557 594 1057 432 747 268 1117
Fragments arranged by size:
1117 1057 747 594 557 432 268
AflII C'TTAA_G
Cuts at: 330 330
Size: 4772
AflIII A'CryG_T
Cuts at: 610 2124 3228 4205 610
Size: 1514 1104 977 1177
Fragments arranged by size:
1514 1177 1104 977
AgeI A'CCGG_T
Cuts at: 3160 3160
Size: 4772
AleI CACnn'nnGTG
Cuts at: 925 925
Size: 4772
AlwNI CAG_nnn'CTG
Cuts at: 969 2077 3644 969
Size: 1108 1567 2097
Fragments arranged by size:
2097 1567 1108
ApaLI G'TGCA_C
Cuts at: 3542 3542
Size: 4772
ApoI r'AATT_y
Cuts at: 653 1743 1776 4125 4381 653
Size: 1090 33 2349 256 1044
Fragments arranged by size:
2349 1090 1044 256 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 4772
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
AvaI C'yCGr_G
Cuts at: 1792 1792
Size: 4772
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 287 320 949 982 1299 1332 287
Size: 33 629 33 317 33 3727
Fragments arranged by size:
3727 629 317 33 33 33
BamHI G'GATC_C
Cuts at: 1360 1797 1360
Size: 437 4335
BanI G'GyrC_C
Cuts at: 705 1399 1788 2202 2450 2485 705
Size: 694 389 414 248 35 2992
Fragments arranged by size:
2992 694 414 389 248 35
BanII G_rGCy'C
Cuts at: 1610 1786 2816 1610
Size: 176 1030 3566
Fragments arranged by size:
3566 1030 176
BbeI G_GCGC'C
Cuts at: 2206 2454 2206
Size: 248 4524
BbvCI CC'TCA_GC
Cuts at: 367 367
Size: 4772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4738
BciVI GTATCCnnnnn_n'
Cuts at: 1327 2670 3431 4772 1327
Size: 1343 761 1341 1327
Fragments arranged by size:
1343 1341 1327 761
BclI T'GATC_A
Cuts at: 1031 2292 1031
Size: 1261 3511
BglII A'GATC_T
Cuts at: 1283 1700 2287 4324 1283
Size: 417 587 2037 1731
Fragments arranged by size:
2037 1731 587 417
BlpI GC'TnA_GC
Cuts at: 1884 1884
Size: 4772
Bme1580I G_kGCm'C
Cuts at: 1681 2397 2490 3546 1681
Size: 716 93 1056 2907
Fragments arranged by size:
2907 1056 716 93
BmrI ACTGGGnnnn_n'
Cuts at: 254 329 764 873 1443 1984 2065 2400 4544
Size: 75 435 109 570 541 81 335 2144
Cuts at: 4544 254
Size: 482
Fragments arranged by size:
2144 570 541 482 435 335 109 81
75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 279 3139 279
Size: 2860 1912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 1174 1624 156
Size: 1018 450 3304
Fragments arranged by size:
3304 1018 450
Bpu10I CC'TnA_GC
Cuts at: 367 367
Size: 4772
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1948 2827 3319 3617 1948
Size: 879 492 298 3103
Fragments arranged by size:
3103 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 174 4476 174
Size: 4302 470
BsaAI yAC'GTr
Cuts at: 2125 2125
Size: 4772
BsaBI GATnn'nnATC
Cuts at: 6 2286 4323 6
Size: 2280 2037 455
Fragments arranged by size:
2280 2037 455
BsaHI Gr'CG_yC
Cuts at: 2203 2451 3153 4649 2203
Size: 248 702 1496 2326
Fragments arranged by size:
2326 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1961 2482 3160 3434 3581 3992 1961
Size: 521 678 274 147 411 2741
Fragments arranged by size:
2741 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 482 503 512 533 1126 1156 482
Size: 21 9 21 593 30 4098
Fragments arranged by size:
4098 593 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 553 553
Size: 4772
BseYI C'CCAG_C
Cuts at: 1609 2197 2955 3532 4028 1609
Size: 588 758 577 496 2353
Fragments arranged by size:
2353 758 588 577 496
BsiEI CG_ry'CG
Cuts at: 104 134 2314 2360 3568 104
Size: 30 2180 46 1208 1308
Fragments arranged by size:
2180 1308 1208 46 30
BsiHKAI G_wGCw'C
Cuts at: 1786 2564 2754 3546 1786
Size: 778 190 792 3012
Fragments arranged by size:
3012 792 778 190
BsmI GAATG_Cn'
Cuts at: 1638 1638
Size: 4772
Bsp1286I G_dGCh'C
Cuts at: 1610 1681 1786 2397 2490 2564 2754 2816 3546
Size: 71 105 611 93 74 190 62 730
Cuts at: 3546 1610
Size: 2836
Fragments arranged by size:
2836 730 611 190 105 93 74 71
62
BspEI T'CCGG_A
Cuts at: 3992 3992
Size: 4772
BspHI T'CATG_A
Cuts at: 3948 3948
Size: 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1380 1387 1822 2036 2338 2719 1380
Size: 7 435 214 302 381 3433
Fragments arranged by size:
3433 435 381 302 214 7
BsrBI CCG'CTC
Cuts at: 50 3064 3118 4602 4767 50
Size: 3014 54 1484 165 55
Fragments arranged by size:
3014 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 197 2684 197
Size: 2487 2285
BsrFI r'CCGG_y
Cuts at: 2770 2951 3160 2770
Size: 181 209 4382
Fragments arranged by size:
4382 209 181
BssSI C'ACGA_G
Cuts at: 1352 3043 3401 1352
Size: 1691 358 2723
Fragments arranged by size:
2723 1691 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2149 2149
Size: 4772
BstBI TT'CG_AA
Cuts at: 3133 3133
Size: 4772
BstEII G'GTnAC_C
Cuts at: 742 742
Size: 4772
BstXI CCAn_nnnn'nTGG
Cuts at: 1410 1410
Size: 4772
BstYI r'GATC_y
Cuts at: 1 811 865 1283 1360 1700 1797 2264 2287
Size: 810 54 418 77 340 97 467 23
Cuts at: 2287 2622 2868 3880 3966 3978 4324 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 810 467 449 418 346 340 335
246 97 86 77 54 23 12
BtgI C'CryG_G
Cuts at: 134 992 1541 134
Size: 858 549 3365
Fragments arranged by size:
3365 858 549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2722 2903 3066 2722
Size: 181 163 4428
Fragments arranged by size:
4428 181 163
BtsI GCAGTG_nn'
Cuts at: 2015 2015
Size: 4772
DraI TTT'AAA
Cuts at: 1771 1771
Size: 4772
DrdI GACnn_nn'nnGTC
Cuts at: 1103 2478 3336 1103
Size: 1375 858 2539
Fragments arranged by size:
2539 1375 858
EaeI y'GGCC_r
Cuts at: 131 2357 2531 2922 2949 4294 131
Size: 2226 174 391 27 1345 609
Fragments arranged by size:
2226 1345 609 391 174 27
EagI C'GGCC_G
Cuts at: 131 2357 131
Size: 2226 2546
EarI CTCTTCn'nnn_
Cuts at: 269 496 883 2795 3005 269
Size: 227 387 1912 210 2036
Fragments arranged by size:
2036 1912 387 227 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1081 1309 2980 3290 3436 4582 1081
Size: 228 1671 310 146 1146 1271
Fragments arranged by size:
1671 1271 1146 310 228 146
EcoICRI GAG'CTC
Cuts at: 1784 1784
Size: 4772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 389 946 1174 1540 1624 2597 3029 3776
Size: 233 557 228 366 84 973 432 747
Cuts at: 3776 4044 156
Size: 268 884
Fragments arranged by size:
973 884 747 557 432 366 268 233
228 84
EcoO109I rG'GnC_Cy
Cuts at: 1911 1911
Size: 4772
EcoRI G'AATT_C
Cuts at: 1776 1776
Size: 4772
EcoRV GAT'ATC
Cuts at: 1591 1591
Size: 4772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 4772
FspI TGC'GCA
Cuts at: 2553 2553
Size: 4772
HaeII r_GCGC'y
Cuts at: 2206 2454 3476 4455 2206
Size: 248 1022 979 2523
Fragments arranged by size:
2523 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1033 1065 2614 2644 2646 2676 1033
Size: 32 1549 30 2 30 3129
Fragments arranged by size:
3129 1549 32 30 30 2
HincII GTy'rAC
Cuts at: 1811 1811
Size: 4772
KasI G'GCGC_C
Cuts at: 2202 2450 2202
Size: 248 4524
KpnI G_GTAC'C
Cuts at: 1403 1792 1403
Size: 389 4383
MfeI C'AATT_G
Cuts at: 596 710 767 596
Size: 114 57 4601
Fragments arranged by size:
4601 114 57
MluI A'CGCG_T
Cuts at: 610 4205 610
Size: 3595 1177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1276 1338 3443 3627 1276
Size: 62 2105 184 2421
Fragments arranged by size:
2421 2105 184 62
MscI TGG'CCA
Cuts at: 2533 2533
Size: 4772
MslI CAynn'nnrTG
Cuts at: 925 928 2761 2888 925
Size: 3 1833 127 2809
Fragments arranged by size:
2809 1833 127 3
MspA1I CmG'CkG
Cuts at: 1366 1883 2197 2557 3570 3815 1366
Size: 517 314 360 1013 245 2323
Fragments arranged by size:
2323 1013 517 360 314 245
NaeI GCC'GGC
Cuts at: 2953 2953
Size: 4772
NarI GG'CG_CC
Cuts at: 2203 2451 2203
Size: 248 4524
NcoI C'CATG_G
Cuts at: 992 1541 992
Size: 549 4223
NgoMIV G'CCGG_C
Cuts at: 2951 2951
Size: 4772
NspI r_CATG'y
Cuts at: 1027 1825 2325 3232 1027
Size: 798 500 907 2567
Fragments arranged by size:
2567 907 798 500
PciI A'CATG_T
Cuts at: 3228 3228
Size: 4772
PmeI GTTT'AAAC
Cuts at: 1771 1771
Size: 4772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4740
PstI C_TGCA'G
Cuts at: 1819 2504 1819
Size: 685 4087
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4772
PvuII CAG'CTG
Cuts at: 2197 2557 2197
Size: 360 4412
RsrII CG'GwC_CG
Cuts at: 2967 2967
Size: 4772
SacI G_AGCT'C
Cuts at: 1786 1786
Size: 4772
SalI G'TCGA_C
Cuts at: 1809 1809
Size: 4772
SapI GCTCTTCn'nnn_
Cuts at: 269 2795 3005 269
Size: 2526 210 2036
Fragments arranged by size:
2526 2036 210
SbfI CC_TGCA'GG
Cuts at: 1819 1819
Size: 4772
ScaI AGT'ACT
Cuts at: 4289 4289
Size: 4772
SfcI C'TryA_G
Cuts at: 33 1815 2500 3493 3684 33
Size: 1782 685 993 191 1121
Fragments arranged by size:
1782 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2204 2452 2204
Size: 248 4524
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4772
SmaI CCC'GGG
Cuts at: 1794 1794
Size: 4772
SmlI C'TyrA_G
Cuts at: 330 1927 2842 3334 3596 330
Size: 1597 915 492 262 1506
Fragments arranged by size:
1597 1506 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4772
SphI G_CATG'C
Cuts at: 1825 2325 1825
Size: 500 4272
StuI AGG'CCT
Cuts at: 1114 1114
Size: 4772
StyI C'CwwG_G
Cuts at: 567 992 1541 1906 2260 567
Size: 425 549 365 354 3079
Fragments arranged by size:
3079 549 425 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 761 2355 2488 2947 3156 761
Size: 1594 133 459 209 2377
Fragments arranged by size:
2377 1594 459 209 133
TatI w'GTAC_w
Cuts at: 833 917 4287 833
Size: 84 3370 1318
Fragments arranged by size:
3370 1318 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 482 503 514 535 4289 4321 482
Size: 21 11 21 3754 32 933
Fragments arranged by size:
3754 933 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 2569 2569
Size: 4772
XbaI T'CTAG_A
Cuts at: 1803 1803
Size: 4772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 180 2267 180
Size: 2087 2685
XmaI C'CCGG_G
Cuts at: 1792 1792
Size: 4772
XmnI GAAnn'nnTTC
Cuts at: 799 1636 799
Size: 837 3935
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BbsI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI