Restriction map for
pF1KB8134
(Circular) (Six-base) MAPSORT of: pF1KB8134.seq Check: 6300 from: 1 to: 3959
pF1KB8134 3959 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 274 274
Size: 3959
AccI GT'mk_AC
Cuts at: 182 997 182
Size: 815 3144
Acc65I G'GTAC_C
Cuts at: 779 975 779
Size: 196 3763
AclI AA'CG_TT
Cuts at: 3799 3799
Size: 3959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1784 2216 2963 3231 877
Size: 907 432 747 268 1605
Fragments arranged by size:
1605 907 747 432 268
AflIII A'CryG_T
Cuts at: 1311 2415 3392 1311
Size: 1104 977 1878
Fragments arranged by size:
1878 1104 977
AgeI A'CCGG_T
Cuts at: 2347 2347
Size: 3959
AlwNI CAG_nnn'CTG
Cuts at: 834 1264 2831 834
Size: 430 1567 1962
Fragments arranged by size:
1962 1567 430
ApaI G_GGCC'C
Cuts at: 150 150
Size: 3959
ApaLI G'TGCA_C
Cuts at: 2729 2729
Size: 3959
ApoI r'AATT_y
Cuts at: 963 3312 3568 963
Size: 2349 256 1354
Fragments arranged by size:
2349 1354 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3959
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
AvaI C'yCGr_G
Cuts at: 725 979 725
Size: 254 3705
BamHI G'GATC_C
Cuts at: 984 984
Size: 3959
BanI G'GyrC_C
Cuts at: 364 779 975 1389 1637 1672 364
Size: 415 196 414 248 35 2651
Fragments arranged by size:
2651 415 414 248 196 35
BanII G_rGCy'C
Cuts at: 150 246 278 973 2003 150
Size: 96 32 695 1030 2106
Fragments arranged by size:
2106 1030 695 96 32
BbeI G_GCGC'C
Cuts at: 1393 1641 1393
Size: 248 3711
BbsI GAAGACnn'nnnn_
Cuts at: 452 452
Size: 3959
BciVI GTATCCnnnnn_n'
Cuts at: 570 1857 2618 3959 570
Size: 1287 761 1341 570
Fragments arranged by size:
1341 1287 761 570
BclI T'GATC_A
Cuts at: 1479 1479
Size: 3959
BglI GCCn_nnn'nGGC
Cuts at: 217 217
Size: 3959
BglII A'GATC_T
Cuts at: 1474 3511 1474
Size: 2037 1922
BlpI GC'TnA_GC
Cuts at: 1071 1071
Size: 3959
Bme1580I G_kGCm'C
Cuts at: 150 1584 1677 2733 150
Size: 1434 93 1056 1376
Fragments arranged by size:
1434 1376 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1171 1252 1587 3731 1171
Size: 81 335 2144 1399
Fragments arranged by size:
2144 1399 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 3959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 268 364 835 856 154
Size: 114 96 471 21 3257
Fragments arranged by size:
3257 471 114 96 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1135 2014 2506 2804 406
Size: 729 879 492 298 1561
Fragments arranged by size:
1561 879 729 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 746 3663 746
Size: 2917 1042
BsaAI yAC'GTr
Cuts at: 1312 1312
Size: 3959
BsaBI GATnn'nnATC
Cuts at: 6 1473 3510 6
Size: 1467 2037 455
Fragments arranged by size:
2037 1467 455
BsaHI Gr'CG_yC
Cuts at: 1390 1638 2340 3836 1390
Size: 248 702 1496 1513
Fragments arranged by size:
1513 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1148 1669 2347 2621 2768 3179 1148
Size: 521 678 274 147 411 1928
Fragments arranged by size:
1928 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 808 838 808
Size: 30 3929
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 409 659 325
Size: 84 250 3625
Fragments arranged by size:
3625 250 84
BseYI C'CCAG_C
Cuts at: 156 168 560 1384 2142 2719 3215 156
Size: 12 392 824 758 577 496 900
Fragments arranged by size:
900 824 758 577 496 392 12
BsiEI CG_ry'CG
Cuts at: 104 1501 1547 2755 104
Size: 1397 46 1208 1308
Fragments arranged by size:
1397 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 973 1751 1941 2733 973
Size: 778 190 792 2199
Fragments arranged by size:
2199 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 150 246 278 973 1584 1677 1751 1941 2003
Size: 96 32 695 611 93 74 190 62
Cuts at: 2003 2733 150
Size: 730 1376
Fragments arranged by size:
1376 730 695 611 190 96 93 74
62 32
BspEI T'CCGG_A
Cuts at: 3179 3179
Size: 3959
BspHI T'CATG_A
Cuts at: 3135 3135
Size: 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 274 914 1009 1223 1525 1906 274
Size: 640 95 214 302 381 2327
Fragments arranged by size:
2327 640 381 302 214 95
BsrBI CCG'CTC
Cuts at: 50 2251 2305 3789 3954 50
Size: 2201 54 1484 165 55
Fragments arranged by size:
2201 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1871 1871
Size: 3959
BsrFI r'CCGG_y
Cuts at: 1957 2138 2347 1957
Size: 181 209 3569
Fragments arranged by size:
3569 209 181
BssSI C'ACGA_G
Cuts at: 2230 2588 2230
Size: 358 3601
BstAPI GCAn_nnn'nTGC
Cuts at: 1336 1336
Size: 3959
BstBI TT'CG_AA
Cuts at: 2320 2320
Size: 3959
BstYI r'GATC_y
Cuts at: 1 887 984 1451 1474 1809 2055 3067 3153
Size: 886 97 467 23 335 246 1012 86
Cuts at: 3153 3165 3511 1
Size: 12 346 449
Fragments arranged by size:
1012 886 467 449 346 335 246 97
86 23 12
BtgI C'CryG_G
Cuts at: 875 875
Size: 3959
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1909 2090 2253 1909
Size: 181 163 3615
Fragments arranged by size:
3615 181 163
BtsI GCAGTG_nn'
Cuts at: 1202 1202
Size: 3959
DraI TTT'AAA
Cuts at: 958 958
Size: 3959
DrdI GACnn_nn'nnGTC
Cuts at: 1665 2523 1665
Size: 858 3101
EaeI y'GGCC_r
Cuts at: 593 1544 1718 2109 2136 3481 593
Size: 951 174 391 27 1345 1071
Fragments arranged by size:
1345 1071 951 391 174 27
EagI C'GGCC_G
Cuts at: 1544 1544
Size: 3959
EarI CTCTTCn'nnn_
Cuts at: 742 1982 2192 742
Size: 1240 210 2509
Fragments arranged by size:
2509 1240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2167 2477 2623 3769 2167
Size: 310 146 1146 2357
Fragments arranged by size:
2357 1146 310 146
EcoICRI GAG'CTC
Cuts at: 971 971
Size: 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 268 364 835 856 877 1784 2216 2963
Size: 114 96 471 21 21 907 432 747
Cuts at: 2963 3231 154
Size: 268 882
Fragments arranged by size:
907 882 747 471 432 268 114 96
21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 424 424
Size: 3959
EcoO109I rG'GnC_Cy
Cuts at: 146 147 231 810 903 941 1098 146
Size: 1 84 579 93 38 157 3007
Fragments arranged by size:
3007 579 157 93 84 38 1
EcoRI G'AATT_C
Cuts at: 963 963
Size: 3959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3959
FspI TGC'GCA
Cuts at: 1740 1740
Size: 3959
HaeII r_GCGC'y
Cuts at: 1393 1641 2663 3642 1393
Size: 248 1022 979 1710
Fragments arranged by size:
1710 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 627 659 1801 1831 1833 1863 280
Size: 32 315 32 1142 30 2 30 2376
Fragments arranged by size:
2376 1142 315 32 32 30 30 2
HincII GTy'rAC
Cuts at: 998 998
Size: 3959
KasI G'GCGC_C
Cuts at: 1389 1637 1389
Size: 248 3711
KpnI G_GTAC'C
Cuts at: 783 979 783
Size: 196 3763
MluI A'CGCG_T
Cuts at: 3392 3392
Size: 3959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2630 2814 2630
Size: 184 3775
MscI TGG'CCA
Cuts at: 595 1720 595
Size: 1125 2834
MslI CAynn'nnrTG
Cuts at: 1948 2075 1948
Size: 127 3832
MspA1I CmG'CkG
Cuts at: 789 1070 1384 1744 2757 3002 789
Size: 281 314 360 1013 245 1746
Fragments arranged by size:
1746 1013 360 314 281 245
NaeI GCC'GGC
Cuts at: 2140 2140
Size: 3959
NarI GG'CG_CC
Cuts at: 1390 1638 1390
Size: 248 3711
NgoMIV G'CCGG_C
Cuts at: 2138 2138
Size: 3959
NruI TCG'CGA
Cuts at: 851 851
Size: 3959
NspI r_CATG'y
Cuts at: 1012 1512 2419 1012
Size: 500 907 2552
Fragments arranged by size:
2552 907 500
PasI CC'CwG_GG
Cuts at: 126 126
Size: 3959
PciI A'CATG_T
Cuts at: 2415 2415
Size: 3959
PflMI CCAn_nnn'nTGG
Cuts at: 163 834 163
Size: 671 3288
PmeI GTTT'AAAC
Cuts at: 958 958
Size: 3959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3927
PpuMI rG'GwC_Cy
Cuts at: 231 903 941 231
Size: 672 38 3249
Fragments arranged by size:
3249 672 38
PspOMI G'GGCC_C
Cuts at: 146 146
Size: 3959
PstI C_TGCA'G
Cuts at: 1006 1691 1006
Size: 685 3274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3959
PvuII CAG'CTG
Cuts at: 1384 1744 1384
Size: 360 3599
RsrII CG'GwC_CG
Cuts at: 2154 2154
Size: 3959
SacI G_AGCT'C
Cuts at: 973 973
Size: 3959
SalI G'TCGA_C
Cuts at: 996 996
Size: 3959
SapI GCTCTTCn'nnn_
Cuts at: 1982 2192 1982
Size: 210 3749
SbfI CC_TGCA'GG
Cuts at: 1006 1006
Size: 3959
ScaI AGT'ACT
Cuts at: 3476 3476
Size: 3959
SfcI C'TryA_G
Cuts at: 33 1002 1687 2680 2871 33
Size: 969 685 993 191 1121
Fragments arranged by size:
1121 993 969 685 191
SfoI GGC'GCC
Cuts at: 1391 1639 1391
Size: 248 3711
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
SmaI CCC'GGG
Cuts at: 981 981
Size: 3959
SmlI C'TyrA_G
Cuts at: 421 1114 2029 2521 2783 421
Size: 693 915 492 262 1597
Fragments arranged by size:
1597 915 693 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3959
SphI G_CATG'C
Cuts at: 1012 1512 1012
Size: 500 3459
StyI C'CwwG_G
Cuts at: 234 1093 1447 234
Size: 859 354 2746
Fragments arranged by size:
2746 859 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1542 1675 2134 2343 1542
Size: 133 459 209 3158
Fragments arranged by size:
3158 459 209 133
TatI w'GTAC_w
Cuts at: 3474 3474
Size: 3959
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 808 840 3476 3508 808
Size: 32 2636 32 1259
Fragments arranged by size:
2636 1259 32 32
Tth111I GACn'n_nGTC
Cuts at: 1756 1756
Size: 3959
XbaI T'CTAG_A
Cuts at: 990 990
Size: 3959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1454 114
Size: 1340 2619
XmaI C'CCGG_G
Cuts at: 979 979
Size: 3959
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI