Restriction map for
pF1KB8135
(Circular) (Six-base) MAPSORT of: pF1KB8135.seq Check: 1234 from: 1 to: 4604
pF1KB8135 4604 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AatII G_ACGT'C
Cuts at: 1013 1013
Size: 4604
AccI GT'mk_AC
Cuts at: 1642 1642
Size: 4604
Acc65I G'GTAC_C
Cuts at: 1620 1620
Size: 4604
AclI AA'CG_TT
Cuts at: 4444 4444
Size: 4604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1333 2429 2861 3608 3876 292
Size: 1041 1096 432 747 268 1020
Fragments arranged by size:
1096 1041 1020 747 432 268
AflII C'TTAA_G
Cuts at: 771 771
Size: 4604
AflIII A'CryG_T
Cuts at: 282 1956 3060 4037 282
Size: 1674 1104 977 849
Fragments arranged by size:
1674 1104 977 849
AgeI A'CCGG_T
Cuts at: 2992 2992
Size: 4604
AhdI GACnn_n'nnGTC
Cuts at: 957 957
Size: 4604
AleI CACnn'nnGTG
Cuts at: 253 253
Size: 4604
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 253 285 253
Size: 32 4572
AlwNI CAG_nnn'CTG
Cuts at: 1129 1311 1537 1909 3476 1129
Size: 182 226 372 1567 2257
Fragments arranged by size:
2257 1567 372 226 182
ApaLI G'TGCA_C
Cuts at: 1446 3374 1446
Size: 1928 2676
ApoI r'AATT_y
Cuts at: 1608 3957 4213 1608
Size: 2349 256 1999
Fragments arranged by size:
2349 1999 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4604
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
AvaI C'yCGr_G
Cuts at: 121 915 933 1624 121
Size: 794 18 691 3101
Fragments arranged by size:
3101 794 691 18
BamHI G'GATC_C
Cuts at: 1629 1629
Size: 4604
BanI G'GyrC_C
Cuts at: 139 521 1212 1620 2034 2282 2317 139
Size: 382 691 408 414 248 35 2426
Fragments arranged by size:
2426 691 414 408 382 248 35
BanII G_rGCy'C
Cuts at: 122 1618 2648 122
Size: 1496 1030 2078
Fragments arranged by size:
2078 1496 1030
BbeI G_GCGC'C
Cuts at: 143 2038 2286 143
Size: 1895 248 2461
Fragments arranged by size:
2461 1895 248
BbsI GAAGACnn'nnnn_
Cuts at: 234 1005 1321 234
Size: 771 316 3517
Fragments arranged by size:
3517 771 316
BciVI GTATCCnnnnn_n'
Cuts at: 2502 3263 4604 2502
Size: 761 1341 2502
Fragments arranged by size:
2502 1341 761
BclI T'GATC_A
Cuts at: 2124 2124
Size: 4604
BglI GCCn_nnn'nGGC
Cuts at: 112 889 112
Size: 777 3827
BglII A'GATC_T
Cuts at: 2119 4156 2119
Size: 2037 2567
BlpI GC'TnA_GC
Cuts at: 1716 1716
Size: 4604
Bme1580I G_kGCm'C
Cuts at: 128 942 1450 2229 2322 3378 128
Size: 814 508 779 93 1056 1354
Fragments arranged by size:
1354 1056 814 779 508 93
BmgBI CAC'GTC
Cuts at: 251 251
Size: 4604
BmrI ACTGGGnnnn_n'
Cuts at: 492 775 1816 1897 2232 4376 492
Size: 283 1041 81 335 2144 720
Fragments arranged by size:
2144 1041 720 335 283 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 1258
Size: 4604
Bpu10I CC'TnA_GC
Cuts at: 564 1073 564
Size: 509 4095
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1780 2659 3151 3449 1780
Size: 879 492 298 2935
Fragments arranged by size:
2935 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 373 4308 373
Size: 3935 669
BsaAI yAC'GTr
Cuts at: 1566 1957 1566
Size: 391 4213
BsaBI GATnn'nnATC
Cuts at: 6 2118 4155 6
Size: 2112 2037 455
Fragments arranged by size:
2112 2037 455
BsaHI Gr'CG_yC
Cuts at: 140 1010 2035 2283 2985 4481 140
Size: 870 1025 248 702 1496 263
Fragments arranged by size:
1496 1025 870 702 263 248
BsaWI w'CCGG_w
Cuts at: 1793 2314 2992 3266 3413 3824 1793
Size: 521 678 274 147 411 2573
Fragments arranged by size:
2573 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 589 619 1459 1489 589
Size: 30 840 30 3704
Fragments arranged by size:
3704 840 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 422 422
Size: 4604
BseYI C'CCAG_C
Cuts at: 2029 2787 3364 3860 2029
Size: 758 577 496 2773
Fragments arranged by size:
2773 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1066
Size: 4604
BsiEI CG_ry'CG
Cuts at: 104 573 1428 2146 2192 3400 104
Size: 469 855 718 46 1208 1308
Fragments arranged by size:
1308 1208 855 718 469 46
BsiHKAI G_wGCw'C
Cuts at: 1450 1618 2396 2586 3378 1450
Size: 168 778 190 792 2676
Fragments arranged by size:
2676 792 778 190 168
BsiWI C'GTAC_G
Cuts at: 516 516
Size: 4604
BsmI GAATG_Cn'
Cuts at: 668 668
Size: 4604
Bsp1286I G_dGCh'C
Cuts at: 122 128 942 1450 1618 2229 2322 2396 2586
Size: 6 814 508 168 611 93 74 190
Cuts at: 2586 2648 3378 122
Size: 62 730 1348
Fragments arranged by size:
1348 814 730 611 508 190 168 93
74 62 6
BspEI T'CCGG_A
Cuts at: 3824 3824
Size: 4604
BspHI T'CATG_A
Cuts at: 3780 3780
Size: 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1654 1868 2170 2551 1654
Size: 214 302 381 3707
Fragments arranged by size:
3707 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1398 2896 2950 4434 4599 50
Size: 1348 1498 54 1484 165 55
Fragments arranged by size:
1498 1484 1348 165 55 54
BsrDI GCAATG_nn'
Cuts at: 871 2516 871
Size: 1645 2959
BsrFI r'CCGG_y
Cuts at: 1450 2602 2783 2992 1450
Size: 1152 181 209 3062
Fragments arranged by size:
3062 1152 209 181
BsrGI T'GTAC_A
Cuts at: 545 545
Size: 4604
BssSI C'ACGA_G
Cuts at: 2875 3233 2875
Size: 358 4246
BstAPI GCAn_nnn'nTGC
Cuts at: 1454 1981 1454
Size: 527 4077
BstBI TT'CG_AA
Cuts at: 2965 2965
Size: 4604
BstEII G'GTnAC_C
Cuts at: 195 1590 195
Size: 1395 3209
BstXI CCAn_nnnn'nTGG
Cuts at: 255 364 255
Size: 109 4495
BstYI r'GATC_y
Cuts at: 1 713 1379 1629 2096 2119 2454 2700 3712
Size: 712 666 250 467 23 335 246 1012
Cuts at: 3712 3798 3810 4156 1
Size: 86 12 346 449
Fragments arranged by size:
1012 712 666 467 449 346 335 250
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 629 887 962 1252 1595 107
Size: 522 258 75 290 343 3116
Fragments arranged by size:
3116 522 343 290 258 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2554 2735 2898 2554
Size: 181 163 4260
Fragments arranged by size:
4260 181 163
BtsI GCAGTG_nn'
Cuts at: 700 974 1847 700
Size: 274 873 3457
Fragments arranged by size:
3457 873 274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 603 638 603
Size: 35 4569
DraI TTT'AAA
Cuts at: 1603 1603
Size: 4604
DrdI GACnn_nn'nnGTC
Cuts at: 2310 3168 2310
Size: 858 3746
EaeI y'GGCC_r
Cuts at: 212 453 570 1425 1524 2189 2363 2754 2781
Size: 241 117 855 99 665 174 391 27
Cuts at: 2781 4126 212
Size: 1345 690
Fragments arranged by size:
1345 855 690 665 391 241 174 117
99 27
EagI C'GGCC_G
Cuts at: 570 1425 2189 570
Size: 855 764 2985
Fragments arranged by size:
2985 855 764
EarI CTCTTCn'nnn_
Cuts at: 633 1268 2627 2837 633
Size: 635 1359 210 2400
Fragments arranged by size:
2400 1359 635 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 2812 3122 3268 4414 130
Size: 2682 310 146 1146 320
Fragments arranged by size:
2682 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 1616 1616
Size: 4604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1258 1333 2429 2861 3608 3876 292
Size: 966 75 1096 432 747 268 1020
Fragments arranged by size:
1096 1020 966 747 432 268 75
EcoNI CCTnn'n_nnAGG
Cuts at: 703 703
Size: 4604
EcoO109I rG'GnC_Cy
Cuts at: 147 421 486 1743 147
Size: 274 65 1257 3008
Fragments arranged by size:
3008 1257 274 65
EcoRI G'AATT_C
Cuts at: 1608 1608
Size: 4604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4604
FspI TGC'GCA
Cuts at: 2385 2385
Size: 4604
HaeII r_GCGC'y
Cuts at: 143 2038 2286 3308 4287 143
Size: 1895 248 1022 979 460
Fragments arranged by size:
1895 1022 979 460 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 569 601 943 975 2446 2476 2478 2508 569
Size: 32 342 32 1471 30 2 30 2665
Fragments arranged by size:
2665 1471 342 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1163 1284 1419 1467 1643 1163
Size: 121 135 48 176 4124
Fragments arranged by size:
4124 176 135 121 48
HpaI GTT'AAC
Cuts at: 1419 1419
Size: 4604
KasI G'GCGC_C
Cuts at: 139 2034 2282 139
Size: 1895 248 2461
Fragments arranged by size:
2461 1895 248
KpnI G_GTAC'C
Cuts at: 1624 1624
Size: 4604
MluI A'CGCG_T
Cuts at: 4037 4037
Size: 4604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 460 3275 3459 460
Size: 2815 184 1605
Fragments arranged by size:
2815 1605 184
MscI TGG'CCA
Cuts at: 214 2365 214
Size: 2151 2453
MslI CAynn'nnrTG
Cuts at: 133 253 362 2593 2720 133
Size: 120 109 2231 127 2017
Fragments arranged by size:
2231 2017 127 120 109
MspA1I CmG'CkG
Cuts at: 175 536 889 1103 1254 1311 1537 1715 2029
Size: 361 353 214 151 57 226 178 314
Cuts at: 2029 2389 3402 3647 175
Size: 360 1013 245 1132
Fragments arranged by size:
1132 1013 361 360 353 314 245 226
214 178 151 57
NaeI GCC'GGC
Cuts at: 2785 2785
Size: 4604
NarI GG'CG_CC
Cuts at: 140 2035 2283 140
Size: 1895 248 2461
Fragments arranged by size:
2461 1895 248
NcoI C'CATG_G
Cuts at: 107 629 107
Size: 522 4082
NdeI CA'TA_TG
Cuts at: 1519 1519
Size: 4604
NgoMIV G'CCGG_C
Cuts at: 2783 2783
Size: 4604
NspI r_CATG'y
Cuts at: 286 1342 1657 2157 3064 286
Size: 1056 315 500 907 1826
Fragments arranged by size:
1826 1056 907 500 315
PasI CC'CwG_GG
Cuts at: 1002 1002
Size: 4604
PciI A'CATG_T
Cuts at: 282 3060 282
Size: 2778 1826
PfoI T'CCnGG_A
Cuts at: 704 704
Size: 4604
PmeI GTTT'AAAC
Cuts at: 1603 1603
Size: 4604
PmlI CAC'GTG
Cuts at: 1566 1566
Size: 4604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 4572
PpuMI rG'GwC_Cy
Cuts at: 421 486 421
Size: 65 4539
PstI C_TGCA'G
Cuts at: 234 1651 2336 234
Size: 1417 685 2502
Fragments arranged by size:
2502 1417 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4604
PvuII CAG'CTG
Cuts at: 1103 1311 1537 2029 2389 1103
Size: 208 226 492 360 3318
Fragments arranged by size:
3318 492 360 226 208
RsrII CG'GwC_CG
Cuts at: 2799 2799
Size: 4604
SacI G_AGCT'C
Cuts at: 1618 1618
Size: 4604
SacII CC_GC'GG
Cuts at: 890 1255 890
Size: 365 4239
SalI G'TCGA_C
Cuts at: 1641 1641
Size: 4604
SanDI GG'GwC_CC
Cuts at: 486 486
Size: 4604
SapI GCTCTTCn'nnn_
Cuts at: 633 2627 2837 633
Size: 1994 210 2400
Fragments arranged by size:
2400 1994 210
SbfI CC_TGCA'GG
Cuts at: 1651 1651
Size: 4604
ScaI AGT'ACT
Cuts at: 4121 4121
Size: 4604
SfcI C'TryA_G
Cuts at: 33 230 1647 2332 3325 3516 33
Size: 197 1417 685 993 191 1121
Fragments arranged by size:
1417 1121 993 685 197 191
SfoI GGC'GCC
Cuts at: 141 2036 2284 141
Size: 1895 248 2461
Fragments arranged by size:
2461 1895 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4604
SmaI CCC'GGG
Cuts at: 123 1626 123
Size: 1503 3101
SmlI C'TyrA_G
Cuts at: 771 1759 2674 3166 3428 771
Size: 988 915 492 262 1947
Fragments arranged by size:
1947 988 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4604
SphI G_CATG'C
Cuts at: 1342 1657 2157 1342
Size: 315 500 3789
Fragments arranged by size:
3789 500 315
SrfI GCCC'GGGC
Cuts at: 123 123
Size: 4604
StyI C'CwwG_G
Cuts at: 107 629 1738 2092 107
Size: 522 1109 354 2619
Fragments arranged by size:
2619 1109 522 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 968 2187 2320 2779 2988 968
Size: 1219 133 459 209 2584
Fragments arranged by size:
2584 1219 459 209 133
TatI w'GTAC_w
Cuts at: 545 4119 545
Size: 3574 1030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 405 437 861 893 4121 4153 405
Size: 32 424 32 3228 32 856
Fragments arranged by size:
3228 856 424 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 238 1558 2401 238
Size: 1320 843 2441
Fragments arranged by size:
2441 1320 843
XbaI T'CTAG_A
Cuts at: 1635 1635
Size: 4604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 207 463 2099 207
Size: 256 1636 2712
Fragments arranged by size:
2712 1636 256
XmaI C'CCGG_G
Cuts at: 121 1624 121
Size: 1503 3101
ZraI GAC'GTC
Cuts at: 1011 1011
Size: 4604
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AlfI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI BsmBI BssHII BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HindIII MfeI NheI NotI
NruI NsiI PacI PflMI PshAI PsiI PspOMI PspXI
PsrI SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI XmnI