Restriction map for
pF1KB8138
(Circular) (Six-base) MAPSORT of: pF1KB8138.seq Check: 4064 from: 1 to: 4892
pF1KB8138 4892 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 829 845 1576 829
Size: 16 731 4145
Fragments arranged by size:
4145 731 16
AccI GT'mk_AC
Cuts at: 1002 1930 1002
Size: 928 3964
Acc65I G'GTAC_C
Cuts at: 1105 1813 1908 1105
Size: 708 95 4089
Fragments arranged by size:
4089 708 95
AclI AA'CG_TT
Cuts at: 4732 4732
Size: 4892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 3149 3896 4164 2717
Size: 432 747 268 3445
Fragments arranged by size:
3445 747 432 268
AflIII A'CryG_T
Cuts at: 1087 1331 1333 2244 3348 4325 1087
Size: 244 2 911 1104 977 1654
Fragments arranged by size:
1654 1104 977 911 244 2
AgeI A'CCGG_T
Cuts at: 3280 3280
Size: 4892
AhdI GACnn_n'nnGTC
Cuts at: 741 741
Size: 4892
AleI CACnn'nnGTG
Cuts at: 422 1649 422
Size: 1227 3665
AlwNI CAG_nnn'CTG
Cuts at: 230 356 1220 1288 1884 2197 3764 230
Size: 126 864 68 596 313 1567 1358
Fragments arranged by size:
1567 1358 864 596 313 126 68
ApaI G_GGCC'C
Cuts at: 1124 1124
Size: 4892
ApaLI G'TGCA_C
Cuts at: 592 3662 592
Size: 3070 1822
ApoI r'AATT_y
Cuts at: 1896 4245 4501 1896
Size: 2349 256 2287
Fragments arranged by size:
2349 2287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4892
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
AvaI C'yCGr_G
Cuts at: 1479 1912 1479
Size: 433 4459
BamHI G'GATC_C
Cuts at: 1917 1917
Size: 4892
BanI G'GyrC_C
Cuts at: 164 472 610 655 749 1105 1131 1772 1813
Size: 308 138 45 94 356 26 641 41
Cuts at: 1813 1908 2322 2570 2605 164
Size: 95 414 248 35 2451
Fragments arranged by size:
2451 641 414 356 308 248 138 95
94 45 41 35 26
BanII G_rGCy'C
Cuts at: 1124 1906 2936 1124
Size: 782 1030 3080
Fragments arranged by size:
3080 1030 782
BbeI G_GCGC'C
Cuts at: 1776 2326 2574 1776
Size: 550 248 4094
Fragments arranged by size:
4094 550 248
BbsI GAAGACnn'nnnn_
Cuts at: 590 590
Size: 4892
BbvCI CC'TCA_GC
Cuts at: 1162 1343 1162
Size: 181 4711
BciVI GTATCCnnnnn_n'
Cuts at: 2790 3551 4892 2790
Size: 761 1341 2790
Fragments arranged by size:
2790 1341 761
BclI T'GATC_A
Cuts at: 2412 2412
Size: 4892
BglI GCCn_nnn'nGGC
Cuts at: 410 983 1325 1405 1559 410
Size: 573 342 80 154 3743
Fragments arranged by size:
3743 573 342 154 80
BglII A'GATC_T
Cuts at: 2407 4444 2407
Size: 2037 2855
BlpI GC'TnA_GC
Cuts at: 2004 2004
Size: 4892
Bme1580I G_kGCm'C
Cuts at: 169 487 596 658 754 1124 1136 2517 2610
Size: 318 109 62 96 370 12 1381 93
Cuts at: 2610 3666 169
Size: 1056 1395
Fragments arranged by size:
1395 1381 1056 370 318 109 96 93
62 12
BmgBI CAC'GTC
Cuts at: 1253 1662 1253
Size: 409 4483
BmrI ACTGGGnnnn_n'
Cuts at: 776 2104 2185 2520 4664 776
Size: 1328 81 335 2144 1004
Fragments arranged by size:
2144 1328 1004 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3259 3259
Size: 4892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 1405
Size: 4892
Bpu10I CC'TnA_GC
Cuts at: 1162 1343 1162
Size: 181 4711
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1893 2068 2947 3439 3737 1893
Size: 175 879 492 298 3048
Fragments arranged by size:
3048 879 492 298 175
BsaI GGTCTCn'nnnn_
Cuts at: 1611 4596 1611
Size: 2985 1907
BsaAI yAC'GTr
Cuts at: 521 1088 1334 1647 2245 521
Size: 567 246 313 598 3168
Fragments arranged by size:
3168 598 567 313 246
BsaBI GATnn'nnATC
Cuts at: 6 392 2406 4443 6
Size: 386 2014 2037 455
Fragments arranged by size:
2037 2014 455 386
BsaHI Gr'CG_yC
Cuts at: 1754 1773 1850 2323 2571 3273 4769 1754
Size: 19 77 473 248 702 1496 1877
Fragments arranged by size:
1877 1496 702 473 248 77 19
BsaWI w'CCGG_w
Cuts at: 732 2081 2602 3280 3554 3701 4112 732
Size: 1349 521 678 274 147 411 1512
Fragments arranged by size:
1512 1349 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 227 907 1218 227
Size: 680 311 3901
Fragments arranged by size:
3901 680 311
BseYI C'CCAG_C
Cuts at: 125 1152 1496 1613 1727 1844 1866 2317 3075
Size: 1027 344 117 114 117 22 451 758
Cuts at: 3075 3652 4148 125
Size: 577 496 869
Fragments arranged by size:
1027 869 758 577 496 451 344 117
117 114 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 823 446
Size: 377 4515
BsiEI CG_ry'CG
Cuts at: 104 2434 2480 3688 104
Size: 2330 46 1208 1308
Fragments arranged by size:
2330 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 596 1906 2684 2874 3666 596
Size: 1310 778 190 792 1822
Fragments arranged by size:
1822 1310 792 778 190
BsmI GAATG_Cn'
Cuts at: 508 508
Size: 4892
BsmBI CGTCTCn'nnnn_
Cuts at: 342 342
Size: 4892
Bsp1286I G_dGCh'C
Cuts at: 169 487 596 658 754 1124 1136 1906 2517
Size: 318 109 62 96 370 12 770 611
Cuts at: 2517 2610 2684 2874 2936 3666 169
Size: 93 74 190 62 730 1395
Fragments arranged by size:
1395 770 730 611 370 318 190 109
96 93 74 62 62 12
BspEI T'CCGG_A
Cuts at: 732 4112 732
Size: 3380 1512
BspHI T'CATG_A
Cuts at: 4068 4068
Size: 4892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 829 845 1000 1246 1480 1576 1711 1942
Size: 54 16 155 246 234 96 135 231
Cuts at: 1942 2156 2458 2839 775
Size: 214 302 381 2828
Fragments arranged by size:
2828 381 302 246 234 231 214 155
135 96 54 16
BsrBI CCG'CTC
Cuts at: 50 3184 3238 4722 4887 50
Size: 3134 54 1484 165 55
Fragments arranged by size:
3134 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2804 2804
Size: 4892
BsrFI r'CCGG_y
Cuts at: 313 2890 3071 3280 313
Size: 2577 181 209 1925
Fragments arranged by size:
2577 1925 209 181
BssSI C'ACGA_G
Cuts at: 3163 3521 3163
Size: 358 4534
BstAPI GCAn_nnn'nTGC
Cuts at: 2269 2269
Size: 4892
BstBI TT'CG_AA
Cuts at: 500 3253 500
Size: 2753 2139
BstXI CCAn_nnnn'nTGG
Cuts at: 1629 1629
Size: 4892
BstYI r'GATC_y
Cuts at: 1 1917 2384 2407 2742 2988 4000 4086 4098
Size: 1916 467 23 335 246 1012 86 12
Cuts at: 4098 4444 1
Size: 346 449
Fragments arranged by size:
1916 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 374 406 467 650 908 1076 374
Size: 32 61 183 258 168 4190
Fragments arranged by size:
4190 258 183 168 61 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2842 3023 3186 2842
Size: 181 163 4548
Fragments arranged by size:
4548 181 163
BtsI GCAGTG_nn'
Cuts at: 303 396 417 429 594 710 840 1065 1311
Size: 93 21 12 165 116 130 225 246
Cuts at: 1311 1357 1545 1776 2135 303
Size: 46 188 231 359 3060
Fragments arranged by size:
3060 359 246 231 225 188 165 130
116 93 46 21 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1166 1201 1166
Size: 35 4857
DraI TTT'AAA
Cuts at: 1891 1891
Size: 4892
DraIII CAC_nnn'GTG
Cuts at: 863 942 1131 863
Size: 79 189 4624
Fragments arranged by size:
4624 189 79
DrdI GACnn_nn'nnGTC
Cuts at: 2598 3456 2598
Size: 858 4034
EaeI y'GGCC_r
Cuts at: 315 390 2477 2651 3042 3069 4414 315
Size: 75 2087 174 391 27 1345 793
Fragments arranged by size:
2087 1345 793 391 174 75 27
EagI C'GGCC_G
Cuts at: 2477 2477
Size: 4892
EarI CTCTTCn'nnn_
Cuts at: 1183 2915 3125 1183
Size: 1732 210 2950
Fragments arranged by size:
2950 1732 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3100 3410 3556 4702 3100
Size: 310 146 1146 3290
Fragments arranged by size:
3290 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1904 1904
Size: 4892
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2717 3149 3896 4164 1405
Size: 1312 432 747 268 2133
Fragments arranged by size:
2133 1312 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 892 892
Size: 4892
EcoO109I rG'GnC_Cy
Cuts at: 1013 1120 1121 1149 1836 1863 2031 1013
Size: 107 1 28 687 27 168 3874
Fragments arranged by size:
3874 687 168 107 28 27 1
EcoRI G'AATT_C
Cuts at: 1896 1896
Size: 4892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4892
FspI TGC'GCA
Cuts at: 935 1803 1828 2673 935
Size: 868 25 845 3154
Fragments arranged by size:
3154 868 845 25
HaeII r_GCGC'y
Cuts at: 1776 2326 2574 3596 4575 1776
Size: 550 248 1022 979 2093
Fragments arranged by size:
2093 1022 979 550 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 258 290 421 453 727 759 2734 2764 2766
Size: 32 131 32 274 32 1975 30 2
Cuts at: 2766 2796 258
Size: 30 2354
Fragments arranged by size:
2354 1975 274 131 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1931 1931
Size: 4892
KasI G'GCGC_C
Cuts at: 1772 2322 2570 1772
Size: 550 248 4094
Fragments arranged by size:
4094 550 248
KpnI G_GTAC'C
Cuts at: 1109 1817 1912 1109
Size: 708 95 4089
Fragments arranged by size:
4089 708 95
MluI A'CGCG_T
Cuts at: 4325 4325
Size: 4892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3563 3747 3563
Size: 184 4708
MscI TGG'CCA
Cuts at: 392 2653 392
Size: 2261 2631
MslI CAynn'nnrTG
Cuts at: 278 422 967 1649 2881 3008 278
Size: 144 545 682 1232 127 2162
Fragments arranged by size:
2162 1232 682 545 144 127
MspA1I CmG'CkG
Cuts at: 227 638 1078 1220 1454 1799 1881 2003 2317
Size: 411 440 142 234 345 82 122 314
Cuts at: 2317 2677 3690 3935 227
Size: 360 1013 245 1184
Fragments arranged by size:
1184 1013 440 411 360 345 314 245
234 142 122 82
NaeI GCC'GGC
Cuts at: 315 3073 315
Size: 2758 2134
NarI GG'CG_CC
Cuts at: 1773 2323 2571 1773
Size: 550 248 4094
Fragments arranged by size:
4094 550 248
NgoMIV G'CCGG_C
Cuts at: 313 3071 313
Size: 2758 2134
NspI r_CATG'y
Cuts at: 383 1945 2445 3352 383
Size: 1562 500 907 1923
Fragments arranged by size:
1923 1562 907 500
PasI CC'CwG_GG
Cuts at: 1323 1323
Size: 4892
PciI A'CATG_T
Cuts at: 3348 3348
Size: 4892
PflMI CCAn_nnn'nTGG
Cuts at: 666 666
Size: 4892
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4892
PmlI CAC'GTG
Cuts at: 521 1334 1647 521
Size: 813 313 3766
Fragments arranged by size:
3766 813 313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4860
PpuMI rG'GwC_Cy
Cuts at: 1863 1863
Size: 4892
PspOMI G'GGCC_C
Cuts at: 1120 1120
Size: 4892
PstI C_TGCA'G
Cuts at: 424 1939 2624 424
Size: 1515 685 2692
Fragments arranged by size:
2692 1515 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4892
PvuII CAG'CTG
Cuts at: 227 1220 1454 1881 2317 2677 227
Size: 993 234 427 436 360 2442
Fragments arranged by size:
2442 993 436 427 360 234
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4892
SacI G_AGCT'C
Cuts at: 1906 1906
Size: 4892
SacII CC_GC'GG
Cuts at: 1079 1079
Size: 4892
SalI G'TCGA_C
Cuts at: 1929 1929
Size: 4892
SanDI GG'GwC_CC
Cuts at: 1863 1863
Size: 4892
SapI GCTCTTCn'nnn_
Cuts at: 2915 3125 2915
Size: 210 4682
SbfI CC_TGCA'GG
Cuts at: 1939 1939
Size: 4892
ScaI AGT'ACT
Cuts at: 4409 4409
Size: 4892
SexAI A'CCwGG_T
Cuts at: 623 1127 623
Size: 504 4388
SfcI C'TryA_G
Cuts at: 33 222 420 1003 1935 2620 3613 3804 33
Size: 189 198 583 932 685 993 191 1121
Fragments arranged by size:
1121 993 932 685 583 198 191 189
SfoI GGC'GCC
Cuts at: 1774 2324 2572 1774
Size: 550 248 4094
Fragments arranged by size:
4094 550 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
SmaI CCC'GGG
Cuts at: 1914 1914
Size: 4892
SmlI C'TyrA_G
Cuts at: 1872 2047 2962 3454 3716 1872
Size: 175 915 492 262 3048
Fragments arranged by size:
3048 915 492 262 175
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4892
SphI G_CATG'C
Cuts at: 383 1945 2445 383
Size: 1562 500 2830
Fragments arranged by size:
2830 1562 500
StyI C'CwwG_G
Cuts at: 893 2026 2380 893
Size: 1133 354 3405
Fragments arranged by size:
3405 1133 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 117 268 460 663 673 711 1477 1639 2475
Size: 151 192 203 10 38 766 162 836
Cuts at: 2475 2608 3067 3276 117
Size: 133 459 209 1733
Fragments arranged by size:
1733 836 766 459 209 203 192 162
151 133 38 10
TatI w'GTAC_w
Cuts at: 4407 4407
Size: 4892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 398 430 1651 1683 4409 4441 398
Size: 32 1221 32 2726 32 849
Fragments arranged by size:
2726 1221 849 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2689 2689
Size: 4892
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1252 1458 2387 114
Size: 1138 206 929 2619
Fragments arranged by size:
2619 1138 929 206
XmaI C'CCGG_G
Cuts at: 1912 1912
Size: 4892
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsaXI BsiWI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PfoI PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI