Restriction map for
pF1KB8139
(Circular) (Six-base) MAPSORT of: pF1KB8139.seq Check: 7682 from: 1 to: 4091
pF1KB8139 4091 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 394 394
Size: 4091
AccI GT'mk_AC
Cuts at: 1129 1129
Size: 4091
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1916 2348 3095 3363 1916
Size: 432 747 268 2644
Fragments arranged by size:
2644 747 432 268
AflIII A'CryG_T
Cuts at: 557 1443 2547 3524 557
Size: 886 1104 977 1124
Fragments arranged by size:
1124 1104 977 886
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 467 499 467
Size: 32 4059
AlwNI CAG_nnn'CTG
Cuts at: 231 1396 2963 231
Size: 1165 1567 1359
Fragments arranged by size:
1567 1359 1165
ApaI G_GGCC'C
Cuts at: 224 224
Size: 4091
ApaLI G'TGCA_C
Cuts at: 2861 2861
Size: 4091
ApoI r'AATT_y
Cuts at: 1095 3444 3700 1095
Size: 2349 256 1486
Fragments arranged by size:
2349 1486 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 169 1111 169
Size: 942 3149
BamHI G'GATC_C
Cuts at: 1116 1116
Size: 4091
BanI G'GyrC_C
Cuts at: 920 1107 1521 1769 1804 920
Size: 187 414 248 35 3207
Fragments arranged by size:
3207 414 248 187 35
BanII G_rGCy'C
Cuts at: 224 1105 2135 224
Size: 881 1030 2180
Fragments arranged by size:
2180 1030 881
BbeI G_GCGC'C
Cuts at: 1525 1773 1525
Size: 248 3843
BbsI GAAGACnn'nnnn_
Cuts at: 522 522
Size: 4091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 150 184 882 916 150
Size: 34 698 34 3325
Fragments arranged by size:
3325 698 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 251 887 1989 2750 4091 251
Size: 636 1102 761 1341 251
Fragments arranged by size:
1341 1102 761 636 251
BclI T'GATC_A
Cuts at: 1611 1611
Size: 4091
BglI GCCn_nnn'nGGC
Cuts at: 228 269 668 790 228
Size: 41 399 122 3529
Fragments arranged by size:
3529 399 122 41
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 1203 1203
Size: 4091
Bme1580I G_kGCm'C
Cuts at: 224 862 1716 1809 2865 224
Size: 638 854 93 1056 1450
Fragments arranged by size:
1450 1056 854 638 93
BmgBI CAC'GTC
Cuts at: 558 558
Size: 4091
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1384 1719 3863 1303
Size: 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 253 942 195
Size: 58 689 3344
Fragments arranged by size:
3344 689 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2146 2638 2936 1267
Size: 879 492 298 2422
Fragments arranged by size:
2422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 322 3795 322
Size: 3473 618
BsaAI yAC'GTr
Cuts at: 147 376 1444 147
Size: 229 1068 2794
Fragments arranged by size:
2794 1068 229
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 1522 1770 2472 3968 1522
Size: 248 702 1496 1645
Fragments arranged by size:
1645 1496 702 248
BsaWI w'CCGG_w
Cuts at: 179 200 1280 1801 2479 2753 2900 3311 179
Size: 21 1080 521 678 274 147 411 959
Fragments arranged by size:
1080 959 678 521 411 274 147 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 467 497 467
Size: 30 4061
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 1003 424
Size: 579 3512
BseYI C'CCAG_C
Cuts at: 224 718 1045 1516 2274 2851 3347 224
Size: 494 327 471 758 577 496 968
Fragments arranged by size:
968 758 577 496 494 471 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 867 520
Size: 347 3744
BsiEI CG_ry'CG
Cuts at: 104 1633 1679 2887 104
Size: 1529 46 1208 1308
Fragments arranged by size:
1529 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1105 1883 2073 2865 1105
Size: 778 190 792 2331
Fragments arranged by size:
2331 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 642 642
Size: 4091
Bsp1286I G_dGCh'C
Cuts at: 224 862 1105 1716 1809 1883 2073 2135 2865
Size: 638 243 611 93 74 190 62 730
Cuts at: 2865 224
Size: 1450
Fragments arranged by size:
1450 730 638 611 243 190 93 74
62
BspEI T'CCGG_A
Cuts at: 200 3311 200
Size: 3111 980
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 394 1141 1355 1657 2038 394
Size: 747 214 302 381 2447
Fragments arranged by size:
2447 747 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2383 2437 3921 4086 50
Size: 2333 54 1484 165 55
Fragments arranged by size:
2333 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2003 2003
Size: 4091
BsrFI r'CCGG_y
Cuts at: 2089 2270 2479 2089
Size: 181 209 3701
Fragments arranged by size:
3701 209 181
BssSI C'ACGA_G
Cuts at: 589 639 2362 2720 589
Size: 50 1723 358 1960
Fragments arranged by size:
1960 1723 358 50
BstAPI GCAn_nnn'nTGC
Cuts at: 1468 1468
Size: 4091
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 931 931
Size: 4091
BstYI r'GATC_y
Cuts at: 1 173 1116 1583 1606 1941 2187 3199 3285
Size: 172 943 467 23 335 246 1012 86
Cuts at: 3285 3297 3643 1
Size: 12 346 449
Fragments arranged by size:
1012 943 467 449 346 335 246 172
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4091
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 780 930 2041 2222 2385 780
Size: 150 1111 181 163 2486
Fragments arranged by size:
2486 1111 181 163 150
BtsI GCAGTG_nn'
Cuts at: 508 655 915 1068 1334 508
Size: 147 260 153 266 3265
Fragments arranged by size:
3265 266 260 153 147
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 347 669 702 791 1676 1850 2241 2268 3613
Size: 322 33 89 885 174 391 27 1345
Cuts at: 3613 347
Size: 825
Fragments arranged by size:
1345 885 825 391 322 174 89 33
27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 151 2114 2324 151
Size: 1963 210 1918
Fragments arranged by size:
1963 1918 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2299 2609 2755 3901 2299
Size: 310 146 1146 2489
Fragments arranged by size:
2489 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1103 1103
Size: 4091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 195 253 942 1916 2348 3095 3363 195
Size: 58 689 974 432 747 268 923
Fragments arranged by size:
974 923 747 689 432 268 58
EcoO109I rG'GnC_Cy
Cuts at: 220 221 446 816 1230 220
Size: 1 225 370 414 3081
Fragments arranged by size:
3081 414 370 225 1
EcoRI G'AATT_C
Cuts at: 1095 1095
Size: 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 1525 1773 2795 3774 1525
Size: 248 1022 979 1842
Fragments arranged by size:
1842 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 1933 1963 1965 1995 238
Size: 32 1663 30 2 30 2334
Fragments arranged by size:
2334 1663 32 30 30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
KasI G'GCGC_C
Cuts at: 1521 1769 1521
Size: 248 3843
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2762 2946 2762
Size: 184 3907
MscI TGG'CCA
Cuts at: 349 793 1852 349
Size: 444 1059 2588
Fragments arranged by size:
2588 1059 444
MslI CAynn'nnrTG
Cuts at: 895 2080 2207 895
Size: 1185 127 2779
Fragments arranged by size:
2779 1185 127
MspA1I CmG'CkG
Cuts at: 228 1202 1516 1876 2889 3134 228
Size: 974 314 360 1013 245 1185
Fragments arranged by size:
1185 1013 974 360 314 245
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 4091
NarI GG'CG_CC
Cuts at: 1522 1770 1522
Size: 248 3843
NcoI C'CATG_G
Cuts at: 107 107
Size: 4091
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 4091
NspI r_CATG'y
Cuts at: 1144 1644 2551 1144
Size: 500 907 2684
Fragments arranged by size:
2684 907 500
PasI CC'CwG_GG
Cuts at: 493 493
Size: 4091
PciI A'CATG_T
Cuts at: 2547 2547
Size: 4091
PflMI CCAn_nnn'nTGG
Cuts at: 231 231
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 467 499 3230 3262 467
Size: 32 2731 32 1296
Fragments arranged by size:
2731 1296 32 32
PpuMI rG'GwC_Cy
Cuts at: 446 816 446
Size: 370 3721
PshAI GACnn'nnGTC
Cuts at: 464 464
Size: 4091
PspOMI G'GGCC_C
Cuts at: 220 220
Size: 4091
PstI C_TGCA'G
Cuts at: 581 1138 1823 581
Size: 557 685 2849
Fragments arranged by size:
2849 685 557
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 1516 1876 1516
Size: 360 3731
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 1105 1105
Size: 4091
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SanDI GG'GwC_CC
Cuts at: 446 446
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 151 2114 2324 151
Size: 1963 210 1918
Fragments arranged by size:
1963 1918 210
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 3608 3608
Size: 4091
SfcI C'TryA_G
Cuts at: 33 577 1134 1819 2812 3003 33
Size: 544 557 685 993 191 1121
Fragments arranged by size:
1121 993 685 557 544 191
SfoI GGC'GCC
Cuts at: 1523 1771 1523
Size: 248 3843
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 1113 1113
Size: 4091
SmlI C'TyrA_G
Cuts at: 1246 2161 2653 2915 1246
Size: 915 492 262 2422
Fragments arranged by size:
2422 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
StyI C'CwwG_G
Cuts at: 107 278 323 1225 1579 107
Size: 171 45 902 354 2619
Fragments arranged by size:
2619 902 354 171 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 903 1674 1807 2266 2475 601
Size: 302 771 133 459 209 2217
Fragments arranged by size:
2217 771 459 302 209 133
TatI w'GTAC_w
Cuts at: 3606 3606
Size: 4091
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4059
Tth111I GACn'n_nGTC
Cuts at: 130 1888 130
Size: 1758 2333
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1586 1586
Size: 4091
XmaI C'CCGG_G
Cuts at: 1111 1111
Size: 4091
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI