Restriction map for
pF1KB8140
(Circular) (Six-base) MAPSORT of: pF1KB8140.seq Check: 1420 from: 1 to: 3926
pF1KB8140 3926 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 391 391
Size: 3926
AccI GT'mk_AC
Cuts at: 413 964 413
Size: 551 3375
Acc65I G'GTAC_C
Cuts at: 942 942
Size: 3926
AclI AA'CG_TT
Cuts at: 340 3766 340
Size: 3426 500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 494 634 1751 2183 2930 3198 469
Size: 25 140 1117 432 747 268 1197
Fragments arranged by size:
1197 1117 747 432 268 140 25
AflIII A'CryG_T
Cuts at: 1278 2382 3359 1278
Size: 1104 977 1845
Fragments arranged by size:
1845 1104 977
AgeI A'CCGG_T
Cuts at: 2314 2314
Size: 3926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 674 674
Size: 3926
AlwNI CAG_nnn'CTG
Cuts at: 195 696 1231 2798 195
Size: 501 535 1567 1323
Fragments arranged by size:
1567 1323 535 501
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 805 930 3279 3535 805
Size: 125 2349 256 1196
Fragments arranged by size:
2349 1196 256 125
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 946 946
Size: 3926
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 148 157 942 1356 1604 1639 148
Size: 9 785 414 248 35 2435
Fragments arranged by size:
2435 785 414 248 35 9
BanII G_rGCy'C
Cuts at: 877 940 1970 877
Size: 63 1030 2833
Fragments arranged by size:
2833 1030 63
BbeI G_GCGC'C
Cuts at: 1360 1608 1360
Size: 248 3678
BbsI GAAGACnn'nnnn_
Cuts at: 255 500 255
Size: 245 3681
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2585 3926 1824
Size: 761 1341 1824
Fragments arranged by size:
1824 1341 761
BclI T'GATC_A
Cuts at: 1446 1446
Size: 3926
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 776 1038 776
Size: 262 3664
Bme1580I G_kGCm'C
Cuts at: 160 1551 1644 2700 160
Size: 1391 93 1056 1386
Fragments arranged by size:
1391 1386 1056 93
BmgBI CAC'GTC
Cuts at: 870 870
Size: 3926
BmrI ACTGGGnnnn_n'
Cuts at: 1138 1219 1554 3698 1138
Size: 81 335 2144 1366
Fragments arranged by size:
2144 1366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 3926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 859 718
Size: 141 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2473 2771 1102
Size: 879 492 298 2257
Fragments arranged by size:
2257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3630 3630
Size: 3926
BsaAI yAC'GTr
Cuts at: 1279 1279
Size: 3926
BsaBI GATnn'nnATC
Cuts at: 6 1440 3477 6
Size: 1434 2037 455
Fragments arranged by size:
2037 1434 455
BsaHI Gr'CG_yC
Cuts at: 388 1357 1605 2307 3803 388
Size: 969 248 702 1496 511
Fragments arranged by size:
1496 969 702 511 248
BsaWI w'CCGG_w
Cuts at: 278 1115 1636 2314 2588 2735 3146 278
Size: 837 521 678 274 147 411 1058
Fragments arranged by size:
1058 837 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 742 780 1351 2109 2686 3182 742
Size: 38 571 758 577 496 1486
Fragments arranged by size:
1486 758 577 571 496 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 864 563
Size: 301 3625
BsiEI CG_ry'CG
Cuts at: 104 1468 1514 2722 104
Size: 1364 46 1208 1308
Fragments arranged by size:
1364 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 940 1718 1908 2700 940
Size: 778 190 792 2166
Fragments arranged by size:
2166 792 778 190
BsmI GAATG_Cn'
Cuts at: 547 547
Size: 3926
Bsp1286I G_dGCh'C
Cuts at: 160 877 940 1551 1644 1718 1908 1970 2700
Size: 717 63 611 93 74 190 62 730
Cuts at: 2700 160
Size: 1386
Fragments arranged by size:
1386 730 717 611 190 93 74 63
62
BspEI T'CCGG_A
Cuts at: 278 3146 278
Size: 2868 1058
BspHI T'CATG_A
Cuts at: 3102 3102
Size: 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 976 1190 1492 1873 976
Size: 214 302 381 3029
Fragments arranged by size:
3029 381 302 214
BsrBI CCG'CTC
Cuts at: 50 498 2218 2272 3756 3921 50
Size: 448 1720 54 1484 165 55
Fragments arranged by size:
1720 1484 448 165 55 54
BsrDI GCAATG_nn'
Cuts at: 668 1838 668
Size: 1170 2756
BsrFI r'CCGG_y
Cuts at: 1924 2105 2314 1924
Size: 181 209 3536
Fragments arranged by size:
3536 209 181
BssSI C'ACGA_G
Cuts at: 2197 2555 2197
Size: 358 3568
BstAPI GCAn_nnn'nTGC
Cuts at: 195 1303 195
Size: 1108 2818
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 750 750
Size: 3926
BstYI r'GATC_y
Cuts at: 1 951 1418 1441 1776 2022 3034 3120 3132
Size: 950 467 23 335 246 1012 86 12
Cuts at: 3132 3478 1
Size: 346 449
Fragments arranged by size:
1012 950 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 414 414
Size: 3926
BtgI C'CryG_G
Cuts at: 107 116 107
Size: 9 3917
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1876 2057 2220 1876
Size: 181 163 3582
Fragments arranged by size:
3582 181 163
BtsI GCAGTG_nn'
Cuts at: 764 852 1169 764
Size: 88 317 3521
Fragments arranged by size:
3521 317 88
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1632 2490 1632
Size: 858 3068
EaeI y'GGCC_r
Cuts at: 465 1511 1685 2076 2103 3448 465
Size: 1046 174 391 27 1345 943
Fragments arranged by size:
1345 1046 943 391 174 27
EagI C'GGCC_G
Cuts at: 1511 1511
Size: 3926
EarI CTCTTCn'nnn_
Cuts at: 855 1949 2159 855
Size: 1094 210 2622
Fragments arranged by size:
2622 1094 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2134 2444 2590 3736 2134
Size: 310 146 1146 2324
Fragments arranged by size:
2324 1146 310 146
EcoICRI GAG'CTC
Cuts at: 938 938
Size: 3926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 494 634 718 859 1751 2183 2930 3198
Size: 25 140 84 141 892 432 747 268
Cuts at: 3198 469
Size: 1197
Fragments arranged by size:
1197 892 747 432 268 141 140 84
25
EcoO109I rG'GnC_Cy
Cuts at: 1065 1065
Size: 3926
EcoRI G'AATT_C
Cuts at: 805 930 805
Size: 125 3801
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3926
FspI TGC'GCA
Cuts at: 1707 1707
Size: 3926
HaeII r_GCGC'y
Cuts at: 1360 1608 2630 3609 1360
Size: 248 1022 979 1677
Fragments arranged by size:
1677 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 859 891 1768 1798 1800 1830 859
Size: 32 877 30 2 30 2955
Fragments arranged by size:
2955 877 32 30 30 2
HincII GTy'rAC
Cuts at: 362 423 965 362
Size: 61 542 3323
Fragments arranged by size:
3323 542 61
HpaI GTT'AAC
Cuts at: 362 362
Size: 3926
KasI G'GCGC_C
Cuts at: 1356 1604 1356
Size: 248 3678
KpnI G_GTAC'C
Cuts at: 946 946
Size: 3926
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2597 2781 2597
Size: 184 3742
MscI TGG'CCA
Cuts at: 1687 1687
Size: 3926
MslI CAynn'nnrTG
Cuts at: 1915 2042 1915
Size: 127 3799
MspA1I CmG'CkG
Cuts at: 118 404 696 780 1037 1351 1711 2724 2969
Size: 286 292 84 257 314 360 1013 245
Cuts at: 2969 118
Size: 1075
Fragments arranged by size:
1075 1013 360 314 292 286 257 245
84
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 3926
NarI GG'CG_CC
Cuts at: 1357 1605 1357
Size: 248 3678
NcoI C'CATG_G
Cuts at: 107 107
Size: 3926
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 3926
NspI r_CATG'y
Cuts at: 979 1479 2386 979
Size: 500 907 2519
Fragments arranged by size:
2519 907 500
PciI A'CATG_T
Cuts at: 2382 2382
Size: 3926
PflMI CCAn_nnn'nTGG
Cuts at: 894 894
Size: 3926
PfoI T'CCnGG_A
Cuts at: 331 836 887 331
Size: 505 51 3370
Fragments arranged by size:
3370 505 51
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3894
PshAI GACnn'nnGTC
Cuts at: 259 259
Size: 3926
PstI C_TGCA'G
Cuts at: 200 973 1658 200
Size: 773 685 2468
Fragments arranged by size:
2468 773 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 404 696 780 1351 1711 404
Size: 292 84 571 360 2619
Fragments arranged by size:
2619 571 360 292 84
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 940 940
Size: 3926
SacII CC_GC'GG
Cuts at: 119 119
Size: 3926
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
SbfI CC_TGCA'GG
Cuts at: 973 973
Size: 3926
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SfcI C'TryA_G
Cuts at: 33 196 969 1654 2647 2838 33
Size: 163 773 685 993 191 1121
Fragments arranged by size:
1121 993 773 685 191 163
SfoI GGC'GCC
Cuts at: 1358 1606 1358
Size: 248 3678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 948 948
Size: 3926
SmlI C'TyrA_G
Cuts at: 1081 1996 2488 2750 1081
Size: 915 492 262 2257
Fragments arranged by size:
2257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 979 1479 979
Size: 500 3426
StuI AGG'CCT
Cuts at: 368 368
Size: 3926
StyI C'CwwG_G
Cuts at: 107 851 1060 1414 107
Size: 744 209 354 2619
Fragments arranged by size:
2619 744 354 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1509 1642 2101 2310 1509
Size: 133 459 209 3125
Fragments arranged by size:
3125 459 209 133
TatI w'GTAC_w
Cuts at: 3441 3441
Size: 3926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 3894
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1421 1421
Size: 3926
XmaI C'CCGG_G
Cuts at: 946 946
Size: 3926
ZraI GAC'GTC
Cuts at: 389 389
Size: 3926
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI