Restriction map for
pF1KB8141
(Circular) (Six-base) MAPSORT of: pF1KB8141.seq Check: 899 from: 1 to: 3521
pF1KB8141 3521 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 280 559 280
Size: 279 3242
Acc65I G'GTAC_C
Cuts at: 537 537
Size: 3521
AclI AA'CG_TT
Cuts at: 3361 3361
Size: 3521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1346 1778 2525 2793 1346
Size: 432 747 268 2074
Fragments arranged by size:
2074 747 432 268
AfeI AGC'GCT
Cuts at: 195 195
Size: 3521
AflIII A'CryG_T
Cuts at: 873 1977 2954 873
Size: 1104 977 1440
Fragments arranged by size:
1440 1104 977
AgeI A'CCGG_T
Cuts at: 142 1909 142
Size: 1767 1754
AlwNI CAG_nnn'CTG
Cuts at: 826 2393 826
Size: 1567 1954
ApaLI G'TGCA_C
Cuts at: 2291 2291
Size: 3521
ApoI r'AATT_y
Cuts at: 525 2874 3130 525
Size: 2349 256 916
Fragments arranged by size:
2349 916 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3521
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3521
AvaI C'yCGr_G
Cuts at: 238 541 238
Size: 303 3218
BamHI G'GATC_C
Cuts at: 546 546
Size: 3521
BanI G'GyrC_C
Cuts at: 537 951 1199 1234 537
Size: 414 248 35 2824
Fragments arranged by size:
2824 414 248 35
BanII G_rGCy'C
Cuts at: 535 1565 535
Size: 1030 2491
BbeI G_GCGC'C
Cuts at: 955 1203 955
Size: 248 3273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 112 146 270 304 112
Size: 34 124 34 3329
Fragments arranged by size:
3329 124 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 491 1419 2180 3521 491
Size: 928 761 1341 491
Fragments arranged by size:
1341 928 761 491
BclI T'GATC_A
Cuts at: 1041 1041
Size: 3521
BglII A'GATC_T
Cuts at: 1036 3073 1036
Size: 2037 1484
BlpI GC'TnA_GC
Cuts at: 633 633
Size: 3521
Bme1580I G_kGCm'C
Cuts at: 1146 1239 2295 1146
Size: 93 1056 2372
Fragments arranged by size:
2372 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 733 814 1149 3293 733
Size: 81 335 2144 961
Fragments arranged by size:
2144 961 335 81
BmtI G_CTAG'C
Cuts at: 398 398
Size: 3521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1576 2068 2366 697
Size: 879 492 298 1852
Fragments arranged by size:
1852 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3225 3225
Size: 3521
BsaAI yAC'GTr
Cuts at: 874 874
Size: 3521
BsaBI GATnn'nnATC
Cuts at: 6 1035 3072 6
Size: 1029 2037 455
Fragments arranged by size:
2037 1029 455
BsaHI Gr'CG_yC
Cuts at: 952 1200 1902 3398 952
Size: 248 702 1496 1075
Fragments arranged by size:
1496 1075 702 248
BsaWI w'CCGG_w
Cuts at: 142 296 710 1231 1909 2183 2330 2741 142
Size: 154 414 521 678 274 147 411 922
Fragments arranged by size:
922 678 521 414 411 274 154 147
BseYI C'CCAG_C
Cuts at: 946 1704 2281 2777 946
Size: 758 577 496 1690
Fragments arranged by size:
1690 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 284 1063 1109 2317 104
Size: 180 779 46 1208 1308
Fragments arranged by size:
1308 1208 779 180 46
BsiHKAI G_wGCw'C
Cuts at: 535 1313 1503 2295 535
Size: 778 190 792 1761
Fragments arranged by size:
1761 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 535 1146 1239 1313 1503 1565 2295 535
Size: 611 93 74 190 62 730 1761
Fragments arranged by size:
1761 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 296 2741 296
Size: 2445 1076
BspHI T'CATG_A
Cuts at: 2697 2697
Size: 3521
BspMI ACCTGCnnnn'nnnn_
Cuts at: 571 785 1087 1468 571
Size: 214 302 381 2624
Fragments arranged by size:
2624 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1813 1867 3351 3516 50
Size: 1763 54 1484 165 55
Fragments arranged by size:
1763 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1433 1433
Size: 3521
BsrFI r'CCGG_y
Cuts at: 142 205 1519 1700 1909 142
Size: 63 1314 181 209 1754
Fragments arranged by size:
1754 1314 209 181 63
BssSI C'ACGA_G
Cuts at: 1792 2150 1792
Size: 358 3163
BstAPI GCAn_nnn'nTGC
Cuts at: 898 898
Size: 3521
BstBI TT'CG_AA
Cuts at: 422 1882 422
Size: 1460 2061
BstYI r'GATC_y
Cuts at: 1 546 1013 1036 1371 1617 2629 2715 2727
Size: 545 467 23 335 246 1012 86 12
Cuts at: 2727 3073 1
Size: 346 449
Fragments arranged by size:
1012 545 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 355 355
Size: 3521
BtgI C'CryG_G
Cuts at: 107 245 107
Size: 138 3383
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 211 1471 1652 1815 211
Size: 1260 181 163 1917
Fragments arranged by size:
1917 1260 181 163
BtsI GCAGTG_nn'
Cuts at: 764 764
Size: 3521
DraI TTT'AAA
Cuts at: 520 520
Size: 3521
DrdI GACnn_nn'nnGTC
Cuts at: 1227 2085 1227
Size: 858 2663
EaeI y'GGCC_r
Cuts at: 170 1106 1280 1671 1698 3043 170
Size: 936 174 391 27 1345 648
Fragments arranged by size:
1345 936 648 391 174 27
EagI C'GGCC_G
Cuts at: 1106 1106
Size: 3521
EarI CTCTTCn'nnn_
Cuts at: 1544 1754 1544
Size: 210 3311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1729 2039 2185 3331 1729
Size: 310 146 1146 1919
Fragments arranged by size:
1919 1146 310 146
EcoICRI GAG'CTC
Cuts at: 533 533
Size: 3521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1346 1778 2525 2793 1346
Size: 432 747 268 2074
Fragments arranged by size:
2074 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 312 312
Size: 3521
EcoO109I rG'GnC_Cy
Cuts at: 660 660
Size: 3521
EcoRI G'AATT_C
Cuts at: 525 525
Size: 3521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 324 1540 324
Size: 1216 2305
FspI TGC'GCA
Cuts at: 1302 1302
Size: 3521
HaeII r_GCGC'y
Cuts at: 197 253 955 1203 2225 3204 197
Size: 56 702 248 1022 979 514
Fragments arranged by size:
1022 979 702 514 248 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 449 481 1363 1393 1395 1425 449
Size: 32 882 30 2 30 2545
Fragments arranged by size:
2545 882 32 30 30 2
HincII GTy'rAC
Cuts at: 281 560 281
Size: 279 3242
KasI G'GCGC_C
Cuts at: 951 1199 951
Size: 248 3273
KpnI G_GTAC'C
Cuts at: 541 541
Size: 3521
MluI A'CGCG_T
Cuts at: 2954 2954
Size: 3521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2376 2192
Size: 184 3337
MscI TGG'CCA
Cuts at: 172 1282 172
Size: 1110 2411
MslI CAynn'nnrTG
Cuts at: 466 1510 1637 466
Size: 1044 127 2350
Fragments arranged by size:
2350 1044 127
MspA1I CmG'CkG
Cuts at: 168 632 946 1306 2319 2564 168
Size: 464 314 360 1013 245 1125
Fragments arranged by size:
1125 1013 464 360 314 245
NaeI GCC'GGC
Cuts at: 1702 1702
Size: 3521
NarI GG'CG_CC
Cuts at: 952 1200 952
Size: 248 3273
NcoI C'CATG_G
Cuts at: 107 107
Size: 3521
NgoMIV G'CCGG_C
Cuts at: 1700 1700
Size: 3521
NheI G'CTAG_C
Cuts at: 394 394
Size: 3521
NspI r_CATG'y
Cuts at: 574 1074 1981 574
Size: 500 907 2114
Fragments arranged by size:
2114 907 500
PciI A'CATG_T
Cuts at: 1977 1977
Size: 3521
PflMI CCAn_nnn'nTGG
Cuts at: 318 318
Size: 3521
PmeI GTTT'AAAC
Cuts at: 520 520
Size: 3521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2692 2660
Size: 32 3489
PstI C_TGCA'G
Cuts at: 389 568 1253 389
Size: 179 685 2657
Fragments arranged by size:
2657 685 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3521
PvuII CAG'CTG
Cuts at: 168 946 1306 168
Size: 778 360 2383
Fragments arranged by size:
2383 778 360
RsrII CG'GwC_CG
Cuts at: 1716 1716
Size: 3521
SacI G_AGCT'C
Cuts at: 535 535
Size: 3521
SalI G'TCGA_C
Cuts at: 279 558 279
Size: 279 3242
SapI GCTCTTCn'nnn_
Cuts at: 1544 1754 1544
Size: 210 3311
SbfI CC_TGCA'GG
Cuts at: 568 568
Size: 3521
ScaI AGT'ACT
Cuts at: 3038 3038
Size: 3521
SexAI A'CCwGG_T
Cuts at: 407 407
Size: 3521
SfcI C'TryA_G
Cuts at: 33 231 385 564 1249 2242 2433 33
Size: 198 154 179 685 993 191 1121
Fragments arranged by size:
1121 993 685 198 191 179 154
SfoI GGC'GCC
Cuts at: 953 1201 953
Size: 248 3273
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3521
SgrAI Cr'CCGG_yG
Cuts at: 142 142
Size: 3521
SmaI CCC'GGG
Cuts at: 240 543 240
Size: 303 3218
SmlI C'TyrA_G
Cuts at: 676 1591 2083 2345 676
Size: 915 492 262 1852
Fragments arranged by size:
1852 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3521
SphI G_CATG'C
Cuts at: 574 1074 574
Size: 500 3021
StyI C'CwwG_G
Cuts at: 107 655 1009 107
Size: 548 354 2619
Fragments arranged by size:
2619 548 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 1104 1237 1696 1905 298
Size: 806 133 459 209 1914
Fragments arranged by size:
1914 806 459 209 133
TatI w'GTAC_w
Cuts at: 3036 3036
Size: 3521
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3489
Tth111I GACn'n_nGTC
Cuts at: 1318 1318
Size: 3521
XbaI T'CTAG_A
Cuts at: 552 552
Size: 3521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1016 1016
Size: 3521
XmaI C'CCGG_G
Cuts at: 238 541 238
Size: 303 3218
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI