Restriction map for
pF1KB8142
(Circular) (Six-base) MAPSORT of: pF1KB8142.seq Check: 4354 from: 1 to: 4295
pF1KB8142 4295 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 723 723
Size: 4295
AccI GT'mk_AC
Cuts at: 203 1333 203
Size: 1130 3165
Acc65I G'GTAC_C
Cuts at: 614 653 1311 614
Size: 39 658 3598
Fragments arranged by size:
3598 658 39
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 2120 2552 3299 3567 652
Size: 1468 432 747 268 1380
Fragments arranged by size:
1468 1380 747 432 268
AflIII A'CryG_T
Cuts at: 1055 1647 2751 3728 1055
Size: 592 1104 977 1622
Fragments arranged by size:
1622 1104 977 592
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AlwNI CAG_nnn'CTG
Cuts at: 149 630 1050 1600 3167 149
Size: 481 420 550 1567 1277
Fragments arranged by size:
1567 1277 550 481 420
ApaI G_GGCC'C
Cuts at: 1000 1217 1000
Size: 217 4078
ApaLI G'TGCA_C
Cuts at: 3065 3065
Size: 4295
ApoI r'AATT_y
Cuts at: 1299 3648 3904 1299
Size: 2349 256 1690
Fragments arranged by size:
2349 1690 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 1315 1315
Size: 4295
BamHI G'GATC_C
Cuts at: 1320 1320
Size: 4295
BanI G'GyrC_C
Cuts at: 591 614 653 1311 1725 1973 2008 591
Size: 23 39 658 414 248 35 2878
Fragments arranged by size:
2878 658 414 248 39 35 23
BanII G_rGCy'C
Cuts at: 401 1000 1217 1309 2339 401
Size: 599 217 92 1030 2357
Fragments arranged by size:
2357 1030 599 217 92
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BbsI GAAGACnn'nnnn_
Cuts at: 191 912 191
Size: 721 3574
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4261
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2954 4295 2193
Size: 761 1341 2193
Fragments arranged by size:
2193 1341 761
BclI T'GATC_A
Cuts at: 1815 1815
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 386 594 1000 1217 1920 2013 3069 386
Size: 208 406 217 703 93 1056 1612
Fragments arranged by size:
1612 1056 703 406 217 208 93
BmrI ACTGGGnnnn_n'
Cuts at: 417 1507 1588 1923 4067 417
Size: 1090 81 335 2144 645
Fragments arranged by size:
2144 1090 645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 540 865 1137 1229 379
Size: 161 325 272 92 3445
Fragments arranged by size:
3445 325 272 161 92
Bpu10I CC'TnA_GC
Cuts at: 298 298
Size: 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1471 2350 2842 3140 1471
Size: 879 492 298 2626
Fragments arranged by size:
2626 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3999 3999
Size: 4295
BsaAI yAC'GTr
Cuts at: 170 1056 1648 170
Size: 886 592 2817
Fragments arranged by size:
2817 886 592
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 468 1260 1726 1974 2676 4172 468
Size: 792 466 248 702 1496 591
Fragments arranged by size:
1496 792 702 591 466 248
BsaWI w'CCGG_w
Cuts at: 461 1484 2005 2683 2957 3104 3515 461
Size: 1023 521 678 274 147 411 1241
Fragments arranged by size:
1241 1023 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 526 556 526
Size: 30 4265
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1174 1174
Size: 4295
BseYI C'CCAG_C
Cuts at: 309 401 449 488 605 666 1720 2478 3055
Size: 92 48 39 117 61 1054 758 577
Cuts at: 3055 3551 309
Size: 496 1053
Fragments arranged by size:
1054 1053 758 577 496 117 92 61
48 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1036
Size: 4295
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3091 104
Size: 1733 46 1208 1308
Fragments arranged by size:
1733 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1309 2087 2277 3069 1309
Size: 778 190 792 2535
Fragments arranged by size:
2535 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 459 459
Size: 4295
Bsp1286I G_dGCh'C
Cuts at: 386 401 594 1000 1217 1309 1920 2013 2087
Size: 15 193 406 217 92 611 93 74
Cuts at: 2087 2277 2339 3069 386
Size: 190 62 730 1612
Fragments arranged by size:
1612 730 611 406 217 193 190 93
92 74 62 15
BspEI T'CCGG_A
Cuts at: 461 3515 461
Size: 3054 1241
BspHI T'CATG_A
Cuts at: 1097 3471 1097
Size: 2374 1921
BspMI ACCTGCnnnn'nnnn_
Cuts at: 723 797 848 1345 1559 1861 2242 723
Size: 74 51 497 214 302 381 2776
Fragments arranged by size:
2776 497 381 302 214 74 51
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 302 2293 2474 2683 302
Size: 1991 181 209 1914
Fragments arranged by size:
1991 1914 209 181
BssSI C'ACGA_G
Cuts at: 574 2566 2924 574
Size: 1992 358 1945
Fragments arranged by size:
1992 1945 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1050 1672 1050
Size: 622 3673
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstEII G'GTnAC_C
Cuts at: 152 152
Size: 4295
BstYI r'GATC_y
Cuts at: 1 956 1320 1787 1810 2145 2391 3403 3489
Size: 955 364 467 23 335 246 1012 86
Cuts at: 3489 3501 3847 1
Size: 12 346 449
Fragments arranged by size:
1012 955 467 449 364 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 468 2245 2426 2589 468
Size: 1777 181 163 2174
Fragments arranged by size:
2174 1777 181 163
BtsI GCAGTG_nn'
Cuts at: 682 1538 682
Size: 856 3439
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DraIII CAC_nnn'GTG
Cuts at: 750 750
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 2001 2859 2001
Size: 858 3437
EaeI y'GGCC_r
Cuts at: 195 257 416 1253 1880 2054 2445 2472 3817
Size: 62 159 837 627 174 391 27 1345
Cuts at: 3817 195
Size: 673
Fragments arranged by size:
1345 837 673 627 391 174 159 62
27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 535 904 2318 2528 535
Size: 369 1414 210 2302
Fragments arranged by size:
2302 1414 369 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2503 2813 2959 4105 2503
Size: 310 146 1146 2693
Fragments arranged by size:
2693 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 540 652 865 1137 1229 2120 2552 3299
Size: 161 112 213 272 92 891 432 747
Cuts at: 3299 3567 379
Size: 268 1107
Fragments arranged by size:
1107 891 747 432 272 268 213 161
112 92
EcoNI CCTnn'n_nnAGG
Cuts at: 844 844
Size: 4295
EcoO109I rG'GnC_Cy
Cuts at: 996 997 1213 1434 996
Size: 1 216 221 3857
Fragments arranged by size:
3857 221 216 1
EcoRI G'AATT_C
Cuts at: 1299 1299
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 796 1081 2076 796
Size: 285 995 3015
Fragments arranged by size:
3015 995 285
HaeII r_GCGC'y
Cuts at: 247 1729 1977 2999 3978 247
Size: 1482 248 1022 979 564
Fragments arranged by size:
1482 1022 979 564 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 926 958 2137 2167 2169 2199 926
Size: 32 1179 30 2 30 3022
Fragments arranged by size:
3022 1179 32 30 30 2
HincII GTy'rAC
Cuts at: 864 1334 864
Size: 470 3825
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 618 657 1315 618
Size: 39 658 3598
Fragments arranged by size:
3598 658 39
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3150 2966
Size: 184 4111
MscI TGG'CCA
Cuts at: 259 2056 259
Size: 1797 2498
MslI CAynn'nnrTG
Cuts at: 585 763 2284 2411 585
Size: 178 1521 127 2469
Fragments arranged by size:
2469 1521 178 127
MspA1I CmG'CkG
Cuts at: 405 492 518 1047 1207 1406 1720 2080 3093
Size: 87 26 529 160 199 314 360 1013
Cuts at: 3093 3338 405
Size: 245 1362
Fragments arranged by size:
1362 1013 529 360 314 245 199 160
87 26
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NspI r_CATG'y
Cuts at: 873 1086 1348 1848 2755 873
Size: 213 262 500 907 2413
Fragments arranged by size:
2413 907 500 262 213
PciI A'CATG_T
Cuts at: 2751 2751
Size: 4295
PflMI CCAn_nnn'nTGG
Cuts at: 750 750
Size: 4295
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PmlI CAC'GTG
Cuts at: 170 1056 170
Size: 886 3409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PspOMI G'GGCC_C
Cuts at: 996 1213 996
Size: 217 4078
PstI C_TGCA'G
Cuts at: 443 1046 1190 1342 2027 443
Size: 603 144 152 685 2711
Fragments arranged by size:
2711 685 603 152 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 405 492 518 1047 1207 1720 2080 405
Size: 87 26 529 160 513 360 2620
Fragments arranged by size:
2620 529 513 360 160 87 26
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SfcI C'TryA_G
Cuts at: 33 439 777 1042 1089 1186 1338 2023 3016
Size: 406 338 265 47 97 152 685 993
Cuts at: 3016 3207 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 406 338 265 191 152
97 47
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 1450 2365 2857 3119 1450
Size: 915 492 262 2626
Fragments arranged by size:
2626 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1086 1348 1848 1086
Size: 262 500 3533
Fragments arranged by size:
3533 500 262
StuI AGG'CCT
Cuts at: 850 850
Size: 4295
StyI C'CwwG_G
Cuts at: 392 1429 1783 392
Size: 1037 354 2904
Fragments arranged by size:
2904 1037 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 174 301 552 967 1878 2011 2470 2679 174
Size: 127 251 415 911 133 459 209 1790
Fragments arranged by size:
1790 911 459 415 251 209 133 127
TatI w'GTAC_w
Cuts at: 644 3810 644
Size: 3166 1129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 354 602 644 1161 1790 354
Size: 248 42 517 629 2859
Fragments arranged by size:
2859 629 517 248 42
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
XmnI GAAnn'nnTTC
Cuts at: 367 367
Size: 4295
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI