Restriction map for
pF1KB8143
(Circular) (Six-base) MAPSORT of: pF1KB8143.seq Check: 9435 from: 1 to: 4331
pF1KB8143 4331 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 385 385
Size: 4331
AccI GT'mk_AC
Cuts at: 1369 1369
Size: 4331
Acc65I G'GTAC_C
Cuts at: 286 1347 286
Size: 1061 3270
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 2156 2588 3335 3603 712
Size: 1444 432 747 268 1440
Fragments arranged by size:
1444 1440 747 432 268
AflIII A'CryG_T
Cuts at: 587 1683 2787 3764 587
Size: 1096 1104 977 1154
Fragments arranged by size:
1154 1104 1096 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 787 819 787
Size: 32 4299
AlwNI CAG_nnn'CTG
Cuts at: 176 633 1636 3203 176
Size: 457 1003 1567 1304
Fragments arranged by size:
1567 1304 1003 457
ApaI G_GGCC'C
Cuts at: 974 974
Size: 4331
ApaLI G'TGCA_C
Cuts at: 3101 3101
Size: 4331
ApoI r'AATT_y
Cuts at: 1335 3684 3940 1335
Size: 2349 256 1726
Fragments arranged by size:
2349 1726 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 973 1159 1177 1192 1351 973
Size: 186 18 15 159 3953
Fragments arranged by size:
3953 186 159 18 15
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 286 637 841 848 1347 1761 2009 2044 286
Size: 351 204 7 499 414 248 35 2573
Fragments arranged by size:
2573 499 414 351 248 204 35 7
BanII G_rGCy'C
Cuts at: 917 974 1184 1345 2375 917
Size: 57 210 161 1030 2873
Fragments arranged by size:
2873 1030 210 161 57
BbeI G_GCGC'C
Cuts at: 641 845 852 1765 2013 641
Size: 204 7 913 248 2959
Fragments arranged by size:
2959 913 248 204 7
BbsI GAAGACnn'nnnn_
Cuts at: 1215 1215
Size: 4331
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglII A'GATC_T
Cuts at: 1846 3883 1846
Size: 2037 2294
BlpI GC'TnA_GC
Cuts at: 829 1443 829
Size: 614 3717
Bme1580I G_kGCm'C
Cuts at: 974 1956 2049 3105 974
Size: 982 93 1056 2200
Fragments arranged by size:
2200 1056 982 93
BmgBI CAC'GTC
Cuts at: 183 183
Size: 4331
BmrI ACTGGGnnnn_n'
Cuts at: 518 1543 1624 1959 4103 518
Size: 1025 81 335 2144 746
Fragments arranged by size:
2144 1025 746 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 703 922 1108 222
Size: 481 219 186 3445
Fragments arranged by size:
3445 481 219 186
Bpu10I CC'TnA_GC
Cuts at: 308 308
Size: 4331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2386 2878 3176 1507
Size: 879 492 298 2662
Fragments arranged by size:
2662 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 748 4035 748
Size: 3287 1044
BsaAI yAC'GTr
Cuts at: 1684 1684
Size: 4331
BsaBI GATnn'nnATC
Cuts at: 6 1845 3882 6
Size: 1839 2037 455
Fragments arranged by size:
2037 1839 455
BsaHI Gr'CG_yC
Cuts at: 563 638 842 849 1072 1762 2010 2712 4208
Size: 75 204 7 223 690 248 702 1496
Cuts at: 4208 563
Size: 686
Fragments arranged by size:
1496 702 690 686 248 223 204 75
7
BsaWI w'CCGG_w
Cuts at: 275 534 1520 2041 2719 2993 3140 3551 275
Size: 259 986 521 678 274 147 411 1055
Fragments arranged by size:
1055 986 678 521 411 274 259 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 787 817 787
Size: 30 4301
BseYI C'CCAG_C
Cuts at: 1171 1183 1231 1756 2514 3091 3587 1171
Size: 12 48 525 758 577 496 1915
Fragments arranged by size:
1915 758 577 525 496 48 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4331
BsiEI CG_ry'CG
Cuts at: 104 886 1217 1319 1873 1919 3127 104
Size: 782 331 102 554 46 1208 1308
Fragments arranged by size:
1308 1208 782 554 331 102 46
BsiHKAI G_wGCw'C
Cuts at: 917 1094 1345 2123 2313 3105 917
Size: 177 251 778 190 792 2143
Fragments arranged by size:
2143 792 778 251 190 177
BsmBI CGTCTCn'nnnn_
Cuts at: 679 679
Size: 4331
Bsp1286I G_dGCh'C
Cuts at: 917 974 1094 1184 1345 1956 2049 2123 2313
Size: 57 120 90 161 611 93 74 190
Cuts at: 2313 2375 3105 917
Size: 62 730 2143
Fragments arranged by size:
2143 730 611 190 161 120 93 90
74 62 57
BspEI T'CCGG_A
Cuts at: 534 3551 534
Size: 3017 1314
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 385 1381 1595 1897 2278 385
Size: 996 214 302 381 2438
Fragments arranged by size:
2438 996 381 302 214
BsrBI CCG'CTC
Cuts at: 50 813 2623 2677 4161 4326 50
Size: 763 1810 54 1484 165 55
Fragments arranged by size:
1810 1484 763 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2243 2243
Size: 4331
BsrFI r'CCGG_y
Cuts at: 121 757 797 2329 2510 2719 121
Size: 636 40 1532 181 209 1733
Fragments arranged by size:
1733 1532 636 209 181 40
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 1098 1708 1098
Size: 610 3721
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 681 681
Size: 4331
BstYI r'GATC_y
Cuts at: 1 1356 1823 1846 2181 2427 3439 3525 3537
Size: 1355 467 23 335 246 1012 86 12
Cuts at: 3537 3883 1
Size: 346 449
Fragments arranged by size:
1355 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 509 107
Size: 402 3929
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 480 522 1574 480
Size: 42 1052 3237
Fragments arranged by size:
3237 1052 42
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 503 883 1214 1316 1916 2090 2481 2508 3853
Size: 380 331 102 600 174 391 27 1345
Cuts at: 3853 503
Size: 981
Fragments arranged by size:
1345 981 600 391 380 331 174 102
27
EagI C'GGCC_G
Cuts at: 883 1214 1316 1916 883
Size: 331 102 600 3298
Fragments arranged by size:
3298 600 331 102
EarI CTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2539 2849 2995 4141 2539
Size: 310 146 1146 2729
Fragments arranged by size:
2729 1146 310 146
EcoICRI GAG'CTC
Cuts at: 915 1343 915
Size: 428 3903
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 703 712 922 1108 2156 2588 3335 3603
Size: 481 9 210 186 1048 432 747 268
Cuts at: 3603 222
Size: 950
Fragments arranged by size:
1048 950 747 481 432 268 210 186
9
EcoO109I rG'GnC_Cy
Cuts at: 173 1003 1470 173
Size: 830 467 3034
Fragments arranged by size:
3034 830 467
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 285 717 2350 285
Size: 432 1633 2266
Fragments arranged by size:
2266 1633 432
FspI TGC'GCA
Cuts at: 297 2112 297
Size: 1815 2516
HaeII r_GCGC'y
Cuts at: 584 641 804 836 845 852 1765 2013 3035
Size: 57 163 32 9 7 913 248 1022
Cuts at: 3035 4014 584
Size: 979 901
Fragments arranged by size:
1022 979 913 901 248 163 57 32
9 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2173 2203 2205 2235 2173
Size: 30 2 30 4269
Fragments arranged by size:
4269 30 30 2
HincII GTy'rAC
Cuts at: 1260 1370 1260
Size: 110 4221
KasI G'GCGC_C
Cuts at: 637 841 848 1761 2009 637
Size: 204 7 913 248 2959
Fragments arranged by size:
2959 913 248 204 7
KpnI G_GTAC'C
Cuts at: 290 1351 290
Size: 1061 3270
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 572 3002 3186 572
Size: 2430 184 1717
Fragments arranged by size:
2430 1717 184
MscI TGG'CCA
Cuts at: 2092 2092
Size: 4331
MslI CAynn'nnrTG
Cuts at: 379 2320 2447 379
Size: 1941 127 2263
Fragments arranged by size:
2263 1941 127
MspA1I CmG'CkG
Cuts at: 681 744 828 888 1098 1131 1442 1756 2116
Size: 63 84 60 210 33 311 314 360
Cuts at: 2116 3129 3374 681
Size: 1013 245 1638
Fragments arranged by size:
1638 1013 360 314 311 245 210 84
63 60 33
NaeI GCC'GGC
Cuts at: 123 2512 123
Size: 2389 1942
NarI GG'CG_CC
Cuts at: 638 842 849 1762 2010 638
Size: 204 7 913 248 2959
Fragments arranged by size:
2959 913 248 204 7
NcoI C'CATG_G
Cuts at: 107 107
Size: 4331
NgoMIV G'CCGG_C
Cuts at: 121 2510 121
Size: 2389 1942
NotI GC'GGCC_GC
Cuts at: 883 883
Size: 4331
NspI r_CATG'y
Cuts at: 771 1384 1884 2791 771
Size: 613 500 907 2311
Fragments arranged by size:
2311 907 613 500
PasI CC'CwG_GG
Cuts at: 837 1077 837
Size: 240 4091
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PflMI CCAn_nnn'nTGG
Cuts at: 633 633
Size: 4331
PfoI T'CCnGG_A
Cuts at: 140 1066 140
Size: 926 3405
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 424 456 787 819 3470 3502 424
Size: 32 331 32 2651 32 1253
Fragments arranged by size:
2651 1253 331 32 32 32
PspOMI G'GGCC_C
Cuts at: 970 970
Size: 4331
PstI C_TGCA'G
Cuts at: 1378 2063 1378
Size: 685 3646
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 681 744 828 1756 2116 681
Size: 63 84 928 360 2896
Fragments arranged by size:
2896 928 360 84 63
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 917 1345 917
Size: 428 3903
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 964 3848 964
Size: 2884 1447
SexAI A'CCwGG_T
Cuts at: 329 329
Size: 4331
SfcI C'TryA_G
Cuts at: 33 1374 2059 3052 3243 33
Size: 1341 685 993 191 1121
Fragments arranged by size:
1341 1121 993 685 191
SfoI GGC'GCC
Cuts at: 639 843 850 1763 2011 639
Size: 204 7 913 248 2959
Fragments arranged by size:
2959 913 248 204 7
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 975 1161 1194 1353 975
Size: 186 33 159 3953
Fragments arranged by size:
3953 186 159 33
SmlI C'TyrA_G
Cuts at: 1486 2401 2893 3155 1486
Size: 915 492 262 2662
Fragments arranged by size:
2662 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
SrfI GCCC'GGGC
Cuts at: 1194 1194
Size: 4331
StyI C'CwwG_G
Cuts at: 107 566 806 1465 1819 107
Size: 459 240 659 354 2619
Fragments arranged by size:
2619 659 459 354 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 238 729 1069 1169 1245 1914 2047 2506 2715
Size: 491 340 100 76 669 133 459 209
Cuts at: 2715 238
Size: 1854
Fragments arranged by size:
1854 669 491 459 340 209 133 100
76
TatI w'GTAC_w
Cuts at: 962 3846 962
Size: 2884 1447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4299
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1826 1826
Size: 4331
XmaI C'CCGG_G
Cuts at: 973 1159 1192 1351 973
Size: 186 33 159 3953
Fragments arranged by size:
3953 186 159 33
XmnI GAAnn'nnTTC
Cuts at: 232 268 370 439 232
Size: 36 102 69 4124
Fragments arranged by size:
4124 102 69 36
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BseRI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SspI StuI SwaI XhoI ZraI