Restriction map for
pF1KB8145
(Circular) (Six-base) MAPSORT of: pF1KB8145.seq Check: 1653 from: 1 to: 4037
pF1KB8145 4037 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 315 1075 315
Size: 760 3277
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1862 2294 3041 3309 1862
Size: 432 747 268 2590
Fragments arranged by size:
2590 747 432 268
AflIII A'CryG_T
Cuts at: 440 1389 2493 3470 440
Size: 949 1104 977 1007
Fragments arranged by size:
1104 1007 977 949
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AleI CACnn'nnGTG
Cuts at: 1012 1012
Size: 4037
AlwNI CAG_nnn'CTG
Cuts at: 343 1342 2909 343
Size: 999 1567 1471
Fragments arranged by size:
1567 1471 999
ApaLI G'TGCA_C
Cuts at: 2807 2807
Size: 4037
ApoI r'AATT_y
Cuts at: 580 1041 3390 3646 580
Size: 461 2349 256 971
Fragments arranged by size:
2349 971 461 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 166 1057 166
Size: 891 3146
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 640 784 796 1006 1053 1467 1715 1750 640
Size: 144 12 210 47 414 248 35 2927
Fragments arranged by size:
2927 414 248 210 144 47 35 12
BanII G_rGCy'C
Cuts at: 167 1051 2081 167
Size: 884 1030 2123
Fragments arranged by size:
2123 1030 884
BbeI G_GCGC'C
Cuts at: 1471 1719 1471
Size: 248 3789
BbvCI CC'TCA_GC
Cuts at: 212 212
Size: 4037
BciVI GTATCCnnnnn_n'
Cuts at: 1935 2696 4037 1935
Size: 761 1341 1935
Fragments arranged by size:
1935 1341 761
BclI T'GATC_A
Cuts at: 1557 1557
Size: 4037
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 1149 1149
Size: 4037
Bme1580I G_kGCm'C
Cuts at: 787 1009 1662 1755 2811 787
Size: 222 653 93 1056 2013
Fragments arranged by size:
2013 1056 653 222 93
BmgBI CAC'GTC
Cuts at: 815 815
Size: 4037
BmrI ACTGGGnnnn_n'
Cuts at: 886 1249 1330 1665 3809 886
Size: 363 81 335 2144 1114
Fragments arranged by size:
2144 1114 363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4037
Bpu10I CC'TnA_GC
Cuts at: 212 212
Size: 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 2092 2584 2882 1213
Size: 879 492 298 2368
Fragments arranged by size:
2368 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 389 3741 389
Size: 3352 685
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 231 1551 3588 6
Size: 225 1320 2037 455
Fragments arranged by size:
2037 1320 455 225
BsaHI Gr'CG_yC
Cuts at: 1468 1716 2418 3914 1468
Size: 248 702 1496 1591
Fragments arranged by size:
1591 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 229
Size: 4037
BseYI C'CCAG_C
Cuts at: 908 1462 2220 2797 3293 908
Size: 554 758 577 496 1652
Fragments arranged by size:
1652 758 577 554 496
BsiEI CG_ry'CG
Cuts at: 104 1579 1625 2833 104
Size: 1475 46 1208 1308
Fragments arranged by size:
1475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1051 1829 2019 2811 1051
Size: 778 190 792 2277
Fragments arranged by size:
2277 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 140 140
Size: 4037
Bsp1286I G_dGCh'C
Cuts at: 167 787 1009 1051 1662 1755 1829 2019 2081
Size: 620 222 42 611 93 74 190 62
Cuts at: 2081 2811 167
Size: 730 1393
Fragments arranged by size:
1393 730 620 611 222 190 93 74
62 42
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 3213 3213
Size: 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1020 1087 1301 1603 1984 1020
Size: 67 214 302 381 3073
Fragments arranged by size:
3073 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2329 2383 3867 4032 50
Size: 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1949 1949
Size: 4037
BsrFI r'CCGG_y
Cuts at: 2035 2216 2425 2035
Size: 181 209 3647
Fragments arranged by size:
3647 209 181
BssSI C'ACGA_G
Cuts at: 409 2308 2666 409
Size: 1899 358 1780
Fragments arranged by size:
1899 1780 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1414 1414
Size: 4037
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstEII G'GTnAC_C
Cuts at: 957 957
Size: 4037
BstYI r'GATC_y
Cuts at: 1 461 509 1062 1529 1552 1887 2133 3145
Size: 460 48 553 467 23 335 246 1012
Cuts at: 3145 3231 3243 3589 1
Size: 86 12 346 449
Fragments arranged by size:
1012 553 467 460 449 346 335 246
86 48 23 12
Bsu36I CC'TnA_GG
Cuts at: 353 353
Size: 4037
BtgI C'CryG_G
Cuts at: 107 672 107
Size: 565 3472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 830 1280 830
Size: 450 3587
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 1622 1796 2187 2214 3559 1622
Size: 174 391 27 1345 2100
Fragments arranged by size:
2100 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1622 1622
Size: 4037
EarI CTCTTCn'nnn_
Cuts at: 856 2060 2270 856
Size: 1204 210 2623
Fragments arranged by size:
2623 1204 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 239 2245 2555 2701 3847 239
Size: 2006 310 146 1146 429
Fragments arranged by size:
2006 1146 429 310 146
EcoICRI GAG'CTC
Cuts at: 1049 1049
Size: 4037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1862 2294 3041 3309 1862
Size: 432 747 268 2590
Fragments arranged by size:
2590 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1176 1176
Size: 4037
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 4037
FspI TGC'GCA
Cuts at: 1818 1818
Size: 4037
HaeII r_GCGC'y
Cuts at: 971 1471 1719 2741 3720 971
Size: 500 248 1022 979 1288
Fragments arranged by size:
1288 1022 979 500 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 1879 1909 1911 1941 154
Size: 32 1693 30 2 30 2250
Fragments arranged by size:
2250 1693 32 30 30 2
HincII GTy'rAC
Cuts at: 1076 1076
Size: 4037
HindIII A'AGCT_T
Cuts at: 517 517
Size: 4037
KasI G'GCGC_C
Cuts at: 1467 1715 1467
Size: 248 3789
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 302 2708 2892 302
Size: 2406 184 1447
Fragments arranged by size:
2406 1447 184
MscI TGG'CCA
Cuts at: 1798 1798
Size: 4037
MslI CAynn'nnrTG
Cuts at: 1012 2026 2153 1012
Size: 1014 127 2896
Fragments arranged by size:
2896 1014 127
MspA1I CmG'CkG
Cuts at: 844 946 974 1148 1462 1822 2835 3080 844
Size: 102 28 174 314 360 1013 245 1801
Fragments arranged by size:
1801 1013 360 314 245 174 102 28
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 1468 1716 1468
Size: 248 3789
NcoI C'CATG_G
Cuts at: 107 672 107
Size: 565 3472
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NspI r_CATG'y
Cuts at: 444 1090 1590 2497 444
Size: 646 500 907 1984
Fragments arranged by size:
1984 907 646 500
PciI A'CATG_T
Cuts at: 440 2493 440
Size: 2053 1984
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PshAI GACnn'nnGTC
Cuts at: 988 988
Size: 4037
PstI C_TGCA'G
Cuts at: 1084 1769 1084
Size: 685 3352
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 844 946 1462 1822 844
Size: 102 516 360 3059
Fragments arranged by size:
3059 516 360 102
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 1051 1051
Size: 4037
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 2060 2270 2060
Size: 210 3827
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 1080 1765 2758 2949 33
Size: 1047 685 993 191 1121
Fragments arranged by size:
1121 1047 993 685 191
SfoI GGC'GCC
Cuts at: 1469 1717 1469
Size: 248 3789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 1192 2107 2599 2861 1192
Size: 915 492 262 2368
Fragments arranged by size:
2368 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
StyI C'CwwG_G
Cuts at: 107 672 710 1171 1525 107
Size: 565 38 461 354 2619
Fragments arranged by size:
2619 565 461 354 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1620 1753 2212 2421 1620
Size: 133 459 209 3236
Fragments arranged by size:
3236 459 209 133
TatI w'GTAC_w
Cuts at: 3552 3552
Size: 4037
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4005
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1532 1532
Size: 4037
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
XmnI GAAnn'nnTTC
Cuts at: 431 431
Size: 4037
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsrGI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI