Restriction map for
pF1KB8151
(Circular) (Six-base) MAPSORT of: pF1KB8151.seq Check: 2725 from: 1 to: 4025
pF1KB8151 4025 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1063 1063
Size: 4025
Acc65I G'GTAC_C
Cuts at: 1041 1041
Size: 4025
AclI AA'CG_TT
Cuts at: 445 3865 445
Size: 3420 605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 999 1850 2282 3029 3297 623
Size: 376 851 432 747 268 1351
Fragments arranged by size:
1351 851 747 432 376 268
AflIII A'CryG_T
Cuts at: 975 1377 2481 3458 975
Size: 402 1104 977 1542
Fragments arranged by size:
1542 1104 977 402
AgeI A'CCGG_T
Cuts at: 2413 2413
Size: 4025
AlwNI CAG_nnn'CTG
Cuts at: 744 1330 2897 744
Size: 586 1567 1872
Fragments arranged by size:
1872 1567 586
ApaI G_GGCC'C
Cuts at: 767 818 767
Size: 51 3974
ApaLI G'TGCA_C
Cuts at: 2795 2795
Size: 4025
ApoI r'AATT_y
Cuts at: 412 1029 3378 3634 412
Size: 617 2349 256 803
Fragments arranged by size:
2349 803 617 256
AseI AT'TA_AT
Cuts at: 20 914 20
Size: 894 3131
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 225 238 346 1045 225
Size: 13 108 699 3205
Fragments arranged by size:
3205 699 108 13
BamHI G'GATC_C
Cuts at: 1050 1050
Size: 4025
BanI G'GyrC_C
Cuts at: 318 1041 1455 1703 1738 318
Size: 723 414 248 35 2605
Fragments arranged by size:
2605 723 414 248 35
BanII G_rGCy'C
Cuts at: 358 525 767 818 1039 2069 358
Size: 167 242 51 221 1030 2314
Fragments arranged by size:
2314 1030 242 221 167 51
BbeI G_GCGC'C
Cuts at: 1459 1707 1459
Size: 248 3777
BciVI GTATCCnnnnn_n'
Cuts at: 1923 2684 4025 1923
Size: 761 1341 1923
Fragments arranged by size:
1923 1341 761
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BglII A'GATC_T
Cuts at: 555 1540 3577 555
Size: 985 2037 1003
Fragments arranged by size:
2037 1003 985
BlpI GC'TnA_GC
Cuts at: 253 1137 253
Size: 884 3141
Bme1580I G_kGCm'C
Cuts at: 767 818 1650 1743 2799 767
Size: 51 832 93 1056 1993
Fragments arranged by size:
1993 1056 832 93 51
BmrI ACTGGGnnnn_n'
Cuts at: 159 754 1237 1318 1653 3797 159
Size: 595 483 81 335 2144 387
Fragments arranged by size:
2144 595 483 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 508 2392 508
Size: 1884 2141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 790 345
Size: 445 3580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 1201 2080 2572 2870 603
Size: 598 879 492 298 1758
Fragments arranged by size:
1758 879 598 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 4025
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 1539 3576 6
Size: 1533 2037 455
Fragments arranged by size:
2037 1533 455
BsaHI Gr'CG_yC
Cuts at: 1456 1704 2406 3902 1456
Size: 248 702 1496 1579
Fragments arranged by size:
1579 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1214 1735 2413 2687 2834 3245 1214
Size: 521 678 274 147 411 1994
Fragments arranged by size:
1994 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 496 526 496
Size: 30 3995
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 517 145
Size: 372 3653
BseYI C'CCAG_C
Cuts at: 335 778 1450 2208 2785 3281 335
Size: 443 672 758 577 496 1079
Fragments arranged by size:
1079 758 672 577 496 443
BsiEI CG_ry'CG
Cuts at: 104 1567 1613 2821 104
Size: 1463 46 1208 1308
Fragments arranged by size:
1463 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 232 254 1039 1817 2007 2799 232
Size: 22 785 778 190 792 1458
Fragments arranged by size:
1458 792 785 778 190 22
BsmI GAATG_Cn'
Cuts at: 624 624
Size: 4025
BsmBI CGTCTCn'nnnn_
Cuts at: 264 282 264
Size: 18 4007
Bsp1286I G_dGCh'C
Cuts at: 232 254 358 525 767 818 1039 1650 1743
Size: 22 104 167 242 51 221 611 93
Cuts at: 1743 1817 2007 2069 2799 232
Size: 74 190 62 730 1458
Fragments arranged by size:
1458 730 611 242 221 190 167 104
93 74 62 51 22
BspEI T'CCGG_A
Cuts at: 3245 3245
Size: 4025
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 690 1075 1289 1591 1972 690
Size: 385 214 302 381 2743
Fragments arranged by size:
2743 385 381 302 214
BsrBI CCG'CTC
Cuts at: 50 135 795 2317 2371 3855 4020 50
Size: 85 660 1522 54 1484 165 55
Fragments arranged by size:
1522 1484 660 165 85 55 54
BsrDI GCAATG_nn'
Cuts at: 394 1937 394
Size: 1543 2482
BsrFI r'CCGG_y
Cuts at: 315 2023 2204 2413 315
Size: 1708 181 209 1927
Fragments arranged by size:
1927 1708 209 181
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 1402 1402
Size: 4025
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstEII G'GTnAC_C
Cuts at: 715 715
Size: 4025
BstYI r'GATC_y
Cuts at: 1 466 555 1050 1517 1540 1875 2121 3133
Size: 465 89 495 467 23 335 246 1012
Cuts at: 3133 3219 3231 3577 1
Size: 86 12 346 449
Fragments arranged by size:
1012 495 467 465 449 346 335 246
89 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1975 2156 2319 1975
Size: 181 163 3681
Fragments arranged by size:
3681 181 163
BtsI GCAGTG_nn'
Cuts at: 616 1268 616
Size: 652 3373
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DraIII CAC_nnn'GTG
Cuts at: 207 748 207
Size: 541 3484
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 1610 1784 2175 2202 3547 1610
Size: 174 391 27 1345 2088
Fragments arranged by size:
2088 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1610 1610
Size: 4025
EarI CTCTTCn'nnn_
Cuts at: 599 2048 2258 599
Size: 1449 210 2366
Fragments arranged by size:
2366 1449 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 332 523 678 2233 2543 2689 3835 332
Size: 191 155 1555 310 146 1146 522
Fragments arranged by size:
1555 1146 522 310 191 155 146
EcoICRI GAG'CTC
Cuts at: 1037 1037
Size: 4025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 623 790 999 1850 2282 3029 3297 345
Size: 278 167 209 851 432 747 268 1073
Fragments arranged by size:
1073 851 747 432 278 268 209 167
EcoO109I rG'GnC_Cy
Cuts at: 764 1164 764
Size: 400 3625
EcoRI G'AATT_C
Cuts at: 412 1029 412
Size: 617 3408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 888 2044 888
Size: 1156 2869
FspI TGC'GCA
Cuts at: 1806 1806
Size: 4025
HaeII r_GCGC'y
Cuts at: 184 1459 1707 2729 3708 184
Size: 1275 248 1022 979 501
Fragments arranged by size:
1275 1022 979 501 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1867 1897 1899 1929 1867
Size: 30 2 30 3963
Fragments arranged by size:
3963 30 30 2
HincII GTy'rAC
Cuts at: 786 1064 786
Size: 278 3747
KasI G'GCGC_C
Cuts at: 1455 1703 1455
Size: 248 3777
KpnI G_GTAC'C
Cuts at: 1045 1045
Size: 4025
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2696 2880 2696
Size: 184 3841
MscI TGG'CCA
Cuts at: 1786 1786
Size: 4025
MslI CAynn'nnrTG
Cuts at: 2014 2141 2014
Size: 127 3898
MspA1I CmG'CkG
Cuts at: 198 302 1136 1450 1810 2823 3068 198
Size: 104 834 314 360 1013 245 1155
Fragments arranged by size:
1155 1013 834 360 314 245 104
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 1456 1704 1456
Size: 248 3777
NcoI C'CATG_G
Cuts at: 107 107
Size: 4025
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NspI r_CATG'y
Cuts at: 979 1078 1578 2485 979
Size: 99 500 907 2519
Fragments arranged by size:
2519 907 500 99
PciI A'CATG_T
Cuts at: 975 2481 975
Size: 1506 2519
PflMI CCAn_nnn'nTGG
Cuts at: 748 748
Size: 4025
PfoI T'CCnGG_A
Cuts at: 590 590
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PspOMI G'GGCC_C
Cuts at: 763 814 763
Size: 51 3974
PspXI vC'TCGA_Gb
Cuts at: 225 225
Size: 4025
PstI C_TGCA'G
Cuts at: 191 1072 1757 191
Size: 881 685 2459
Fragments arranged by size:
2459 881 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 1450 1810 1450
Size: 360 3665
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 1039 1039
Size: 4025
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 599 2048 2258 599
Size: 1449 210 2366
Fragments arranged by size:
2366 1449 210
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SfcI C'TryA_G
Cuts at: 33 187 1068 1753 2746 2937 33
Size: 154 881 685 993 191 1121
Fragments arranged by size:
1121 993 881 685 191 154
SfoI GGC'GCC
Cuts at: 1457 1705 1457
Size: 248 3777
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SgrAI Cr'CCGG_yG
Cuts at: 315 315
Size: 4025
SmaI CCC'GGG
Cuts at: 240 348 1047 240
Size: 108 699 3218
Fragments arranged by size:
3218 699 108
SmlI C'TyrA_G
Cuts at: 225 582 1180 2095 2587 2849 225
Size: 357 598 915 492 262 1401
Fragments arranged by size:
1401 915 598 492 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 1078 1578 1078
Size: 500 3525
StyI C'CwwG_G
Cuts at: 107 872 1159 1513 107
Size: 765 287 354 2619
Fragments arranged by size:
2619 765 354 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 736 749 1608 1741 2200 2409 736
Size: 13 859 133 459 209 2352
Fragments arranged by size:
2352 859 459 209 133 13
TatI w'GTAC_w
Cuts at: 3540 3540
Size: 4025
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 925 957 3542 3574 925
Size: 32 2585 32 1376
Fragments arranged by size:
2585 1376 32 32
Tth111I GACn'n_nGTC
Cuts at: 1822 1822
Size: 4025
XbaI T'CTAG_A
Cuts at: 1056 1056
Size: 4025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1520 1520
Size: 4025
XhoI C'TCGA_G
Cuts at: 225 225
Size: 4025
XmaI C'CCGG_G
Cuts at: 238 346 1045 238
Size: 108 699 3218
Fragments arranged by size:
3218 699 108
XmnI GAAnn'nnTTC
Cuts at: 557 557
Size: 4025
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
ZraI