Restriction map for
pF1KB8152
(Circular) (Six-base) MAPSORT of: pF1KB8152.seq Check: 4600 from: 1 to: 3731
pF1KB8152 3731 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 769 769
Size: 3731
Acc65I G'GTAC_C
Cuts at: 747 747
Size: 3731
AclI AA'CG_TT
Cuts at: 3571 3571
Size: 3731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 1556 1988 2735 3003 411
Size: 1145 432 747 268 1139
Fragments arranged by size:
1145 1139 747 432 268
AflIII A'CryG_T
Cuts at: 1083 2187 3164 1083
Size: 1104 977 1650
Fragments arranged by size:
1650 1104 977
AgeI A'CCGG_T
Cuts at: 2119 2119
Size: 3731
AlwNI CAG_nnn'CTG
Cuts at: 1036 2603 1036
Size: 1567 2164
ApaLI G'TGCA_C
Cuts at: 2501 2501
Size: 3731
ApoI r'AATT_y
Cuts at: 135 629 735 3084 3340 135
Size: 494 106 2349 256 526
Fragments arranged by size:
2349 526 494 256 106
AseI AT'TA_AT
Cuts at: 20 20
Size: 3731
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
AvaI C'yCGr_G
Cuts at: 751 751
Size: 3731
BamHI G'GATC_C
Cuts at: 756 756
Size: 3731
BanI G'GyrC_C
Cuts at: 747 1161 1409 1444 747
Size: 414 248 35 3034
Fragments arranged by size:
3034 414 248 35
BanII G_rGCy'C
Cuts at: 745 1775 745
Size: 1030 2701
BbeI G_GCGC'C
Cuts at: 1165 1413 1165
Size: 248 3483
BbsI GAAGACnn'nnnn_
Cuts at: 656 656
Size: 3731
BciVI GTATCCnnnnn_n'
Cuts at: 1629 2390 3731 1629
Size: 761 1341 1629
Fragments arranged by size:
1629 1341 761
BclI T'GATC_A
Cuts at: 1251 1251
Size: 3731
BglII A'GATC_T
Cuts at: 1246 3283 1246
Size: 2037 1694
BlpI GC'TnA_GC
Cuts at: 545 843 545
Size: 298 3433
Bme1580I G_kGCm'C
Cuts at: 1356 1449 2505 1356
Size: 93 1056 2582
Fragments arranged by size:
2582 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 943 1024 1359 3503 943
Size: 81 335 2144 1171
Fragments arranged by size:
2144 1171 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 3731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1786 2278 2576 907
Size: 879 492 298 2062
Fragments arranged by size:
2062 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3435 3435
Size: 3731
BsaAI yAC'GTr
Cuts at: 567 1084 567
Size: 517 3214
BsaBI GATnn'nnATC
Cuts at: 6 301 1245 3282 6
Size: 295 944 2037 455
Fragments arranged by size:
2037 944 455 295
BsaHI Gr'CG_yC
Cuts at: 1162 1410 2112 3608 1162
Size: 248 702 1496 1285
Fragments arranged by size:
1496 1285 702 248
BsaWI w'CCGG_w
Cuts at: 920 1441 2119 2393 2540 2951 920
Size: 521 678 274 147 411 1700
Fragments arranged by size:
1700 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 316 1156 1914 2491 2987 316
Size: 840 758 577 496 1060
Fragments arranged by size:
1060 840 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1273 1319 2527 104
Size: 1169 46 1208 1308
Fragments arranged by size:
1308 1208 1169 46
BsiHKAI G_wGCw'C
Cuts at: 745 1523 1713 2505 745
Size: 778 190 792 1971
Fragments arranged by size:
1971 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 745 1356 1449 1523 1713 1775 2505 745
Size: 611 93 74 190 62 730 1971
Fragments arranged by size:
1971 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2951 2951
Size: 3731
BspHI T'CATG_A
Cuts at: 2907 2907
Size: 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 781 995 1297 1678 781
Size: 214 302 381 2834
Fragments arranged by size:
2834 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2023 2077 3561 3726 50
Size: 1973 54 1484 165 55
Fragments arranged by size:
1973 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1643 1643
Size: 3731
BsrFI r'CCGG_y
Cuts at: 1729 1910 2119 1729
Size: 181 209 3341
Fragments arranged by size:
3341 209 181
BssSI C'ACGA_G
Cuts at: 587 2002 2360 587
Size: 1415 358 1958
Fragments arranged by size:
1958 1415 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1108 1108
Size: 3731
BstBI TT'CG_AA
Cuts at: 2092 2092
Size: 3731
BstYI r'GATC_y
Cuts at: 1 756 1223 1246 1581 1827 2839 2925 2937
Size: 755 467 23 335 246 1012 86 12
Cuts at: 2937 3283 1
Size: 346 449
Fragments arranged by size:
1012 755 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3731
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1681 1862 2025 1681
Size: 181 163 3387
Fragments arranged by size:
3387 181 163
BtsI GCAGTG_nn'
Cuts at: 177 556 974 177
Size: 379 418 2934
Fragments arranged by size:
2934 418 379
ClaI AT'CG_AT
Cuts at: 299 299
Size: 3731
DraI TTT'AAA
Cuts at: 730 730
Size: 3731
DrdI GACnn_nn'nnGTC
Cuts at: 1437 2295 1437
Size: 858 2873
EaeI y'GGCC_r
Cuts at: 1316 1490 1881 1908 3253 1316
Size: 174 391 27 1345 1794
Fragments arranged by size:
1794 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1316 1316
Size: 3731
EarI CTCTTCn'nnn_
Cuts at: 521 673 1754 1964 521
Size: 152 1081 210 2288
Fragments arranged by size:
2288 1081 210 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1939 2249 2395 3541 1939
Size: 310 146 1146 2129
Fragments arranged by size:
2129 1146 310 146
EcoICRI GAG'CTC
Cuts at: 743 743
Size: 3731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 1556 1988 2735 3003 411
Size: 1145 432 747 268 1139
Fragments arranged by size:
1145 1139 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 870 870
Size: 3731
EcoRI G'AATT_C
Cuts at: 135 735 135
Size: 600 3131
EcoRV GAT'ATC
Cuts at: 303 303
Size: 3731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1750 1750
Size: 3731
FspI TGC'GCA
Cuts at: 1512 1512
Size: 3731
HaeII r_GCGC'y
Cuts at: 1165 1413 2435 3414 1165
Size: 248 1022 979 1482
Fragments arranged by size:
1482 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1573 1603 1605 1635 1573
Size: 30 2 30 3669
Fragments arranged by size:
3669 30 30 2
HincII GTy'rAC
Cuts at: 561 600 770 561
Size: 39 170 3522
Fragments arranged by size:
3522 170 39
HpaI GTT'AAC
Cuts at: 561 561
Size: 3731
KasI G'GCGC_C
Cuts at: 1161 1409 1161
Size: 248 3483
KpnI G_GTAC'C
Cuts at: 751 751
Size: 3731
MluI A'CGCG_T
Cuts at: 3164 3164
Size: 3731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2586 2402
Size: 184 3547
MscI TGG'CCA
Cuts at: 1492 1492
Size: 3731
MslI CAynn'nnrTG
Cuts at: 163 1720 1847 163
Size: 1557 127 2047
Fragments arranged by size:
2047 1557 127
MspA1I CmG'CkG
Cuts at: 316 421 842 1156 1516 2529 2774 316
Size: 105 421 314 360 1013 245 1273
Fragments arranged by size:
1273 1013 421 360 314 245 105
NaeI GCC'GGC
Cuts at: 1912 1912
Size: 3731
NarI GG'CG_CC
Cuts at: 1162 1410 1162
Size: 248 3483
NcoI C'CATG_G
Cuts at: 107 107
Size: 3731
NgoMIV G'CCGG_C
Cuts at: 1910 1910
Size: 3731
NspI r_CATG'y
Cuts at: 784 1284 2191 784
Size: 500 907 2324
Fragments arranged by size:
2324 907 500
PciI A'CATG_T
Cuts at: 2187 2187
Size: 3731
PmeI GTTT'AAAC
Cuts at: 730 730
Size: 3731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2902 2870
Size: 32 3699
PstI C_TGCA'G
Cuts at: 778 1463 778
Size: 685 3046
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3731
PvuII CAG'CTG
Cuts at: 316 421 1156 1516 316
Size: 105 735 360 2531
Fragments arranged by size:
2531 735 360 105
RsrII CG'GwC_CG
Cuts at: 1926 1926
Size: 3731
SacI G_AGCT'C
Cuts at: 745 745
Size: 3731
SalI G'TCGA_C
Cuts at: 768 768
Size: 3731
SapI GCTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
SbfI CC_TGCA'GG
Cuts at: 778 778
Size: 3731
ScaI AGT'ACT
Cuts at: 3248 3248
Size: 3731
SfcI C'TryA_G
Cuts at: 33 202 416 521 774 1459 2452 2643 33
Size: 169 214 105 253 685 993 191 1121
Fragments arranged by size:
1121 993 685 253 214 191 169 105
SfoI GGC'GCC
Cuts at: 1163 1411 1163
Size: 248 3483
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
SmaI CCC'GGG
Cuts at: 753 753
Size: 3731
SmlI C'TyrA_G
Cuts at: 886 1801 2293 2555 886
Size: 915 492 262 2062
Fragments arranged by size:
2062 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3731
SphI G_CATG'C
Cuts at: 784 1284 784
Size: 500 3231
StyI C'CwwG_G
Cuts at: 107 125 865 1219 107
Size: 18 740 354 2619
Fragments arranged by size:
2619 740 354 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1314 1447 1906 2115 1314
Size: 133 459 209 2930
Fragments arranged by size:
2930 459 209 133
TatI w'GTAC_w
Cuts at: 3246 3246
Size: 3731
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 3699
Tth111I GACn'n_nGTC
Cuts at: 1528 1528
Size: 3731
XbaI T'CTAG_A
Cuts at: 762 762
Size: 3731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1226 1226
Size: 3731
XmaI C'CCGG_G
Cuts at: 751 751
Size: 3731
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
CspCI DraIII EcoNI FseI FspAI HindIII MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI