Restriction map for
pF1KB8156
(Circular) (Six-base) MAPSORT of: pF1KB8156.seq Check: 9104 from: 1 to: 4325
pF1KB8156 4325 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 390 390
Size: 4325
AatII G_ACGT'C
Cuts at: 1115 1115
Size: 4325
AccI GT'mk_AC
Cuts at: 1363 1363
Size: 4325
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4325
AclI AA'CG_TT
Cuts at: 1319 4165 1319
Size: 2846 1479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 532 2150 2582 3329 3597 184
Size: 348 1618 432 747 268 912
Fragments arranged by size:
1618 912 747 432 348 268
AflIII A'CryG_T
Cuts at: 1677 2781 3758 1677
Size: 1104 977 2244
Fragments arranged by size:
2244 1104 977
AgeI A'CCGG_T
Cuts at: 2713 2713
Size: 4325
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1264 1296 1264
Size: 32 4293
AlwNI CAG_nnn'CTG
Cuts at: 432 1630 3197 432
Size: 1198 1567 1560
Fragments arranged by size:
1567 1560 1198
ApaLI G'TGCA_C
Cuts at: 253 3095 253
Size: 2842 1483
ApoI r'AATT_y
Cuts at: 244 466 1153 1194 1329 3678 3934 244
Size: 222 687 41 135 2349 256 635
Fragments arranged by size:
2349 687 635 256 222 135 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 4325
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
AvaI C'yCGr_G
Cuts at: 121 413 1345 121
Size: 292 932 3101
Fragments arranged by size:
3101 932 292
BamHI G'GATC_C
Cuts at: 1350 1350
Size: 4325
BanI G'GyrC_C
Cuts at: 1341 1755 2003 2038 1341
Size: 414 248 35 3628
Fragments arranged by size:
3628 414 248 35
BanII G_rGCy'C
Cuts at: 289 884 989 1339 2369 289
Size: 595 105 350 1030 2245
Fragments arranged by size:
2245 1030 595 350 105
BbeI G_GCGC'C
Cuts at: 1759 2007 1759
Size: 248 4077
BbsI GAAGACnn'nnnn_
Cuts at: 323 526 323
Size: 203 4122
BbvCI CC'TCA_GC
Cuts at: 1124 1124
Size: 4325
BciVI GTATCCnnnnn_n'
Cuts at: 2223 2984 4325 2223
Size: 761 1341 2223
Fragments arranged by size:
2223 1341 761
BclI T'GATC_A
Cuts at: 612 1845 612
Size: 1233 3092
BglII A'GATC_T
Cuts at: 163 811 1840 3877 163
Size: 648 1029 2037 611
Fragments arranged by size:
2037 1029 648 611
BlpI GC'TnA_GC
Cuts at: 917 1437 917
Size: 520 3805
Bme1580I G_kGCm'C
Cuts at: 257 1950 2043 3099 257
Size: 1693 93 1056 1483
Fragments arranged by size:
1693 1483 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 442 1537 1618 1953 4097 442
Size: 1095 81 335 2144 670
Fragments arranged by size:
2144 1095 670 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 728
Size: 4325
Bpu10I CC'TnA_GC
Cuts at: 1022 1124 1022
Size: 102 4223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 2380 2872 3170 1501
Size: 879 492 298 2656
Fragments arranged by size:
2656 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4029 4029
Size: 4325
BsaAI yAC'GTr
Cuts at: 1678 1678
Size: 4325
BsaBI GATnn'nnATC
Cuts at: 6 168 617 1839 3876 6
Size: 162 449 1222 2037 455
Fragments arranged by size:
2037 1222 455 449 162
BsaHI Gr'CG_yC
Cuts at: 1112 1756 2004 2706 4202 1112
Size: 644 248 702 1496 1235
Fragments arranged by size:
1496 1235 702 644 248
BsaWI w'CCGG_w
Cuts at: 1514 2035 2713 2987 3134 3545 1514
Size: 521 678 274 147 411 2294
Fragments arranged by size:
2294 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1264 1294 1264
Size: 30 4295
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 979 157
Size: 822 3503
BseYI C'CCAG_C
Cuts at: 364 1750 2508 3085 3581 364
Size: 1386 758 577 496 1108
Fragments arranged by size:
1386 1108 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1867 1913 3121 104
Size: 1763 46 1208 1308
Fragments arranged by size:
1763 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 257 989 1339 2117 2307 3099 257
Size: 732 350 778 190 792 1483
Fragments arranged by size:
1483 792 778 732 350 190
Bsp1286I G_dGCh'C
Cuts at: 257 289 884 989 1339 1950 2043 2117 2307
Size: 32 595 105 350 611 93 74 190
Cuts at: 2307 2369 3099 257
Size: 62 730 1483
Fragments arranged by size:
1483 730 611 595 350 190 105 93
74 62 32
BspEI T'CCGG_A
Cuts at: 3545 3545
Size: 4325
BspHI T'CATG_A
Cuts at: 3501 3501
Size: 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 390 1375 1589 1891 2272 390
Size: 985 214 302 381 2443
Fragments arranged by size:
2443 985 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2617 2671 4155 4320 50
Size: 2567 54 1484 165 55
Fragments arranged by size:
2567 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2237 2237
Size: 4325
BsrFI r'CCGG_y
Cuts at: 2323 2504 2713 2323
Size: 181 209 3935
Fragments arranged by size:
3935 209 181
BssSI C'ACGA_G
Cuts at: 2596 2954 2596
Size: 358 3967
BstAPI GCAn_nnn'nTGC
Cuts at: 1702 1702
Size: 4325
BstBI TT'CG_AA
Cuts at: 2686 2686
Size: 4325
BstYI r'GATC_y
Cuts at: 1 163 618 811 1026 1350 1817 1840 2175
Size: 162 455 193 215 324 467 23 335
Cuts at: 2175 2421 3433 3519 3531 3877 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 455 449 346 335 324 246
215 193 162 86 23 12
BtgI C'CryG_G
Cuts at: 107 113 107
Size: 6 4319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2275 2456 2619 2275
Size: 181 163 3981
Fragments arranged by size:
3981 181 163
BtsI GCAGTG_nn'
Cuts at: 268 1568 268
Size: 1300 3025
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4325
DrdI GACnn_nn'nnGTC
Cuts at: 2031 2889 2031
Size: 858 3467
EaeI y'GGCC_r
Cuts at: 110 1910 2084 2475 2502 3847 110
Size: 1800 174 391 27 1345 588
Fragments arranged by size:
1800 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1910 1910
Size: 4325
EarI CTCTTCn'nnn_
Cuts at: 872 1201 2348 2558 872
Size: 329 1147 210 2639
Fragments arranged by size:
2639 1147 329 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 648 2533 2843 2989 4135 648
Size: 1885 310 146 1146 838
Fragments arranged by size:
1885 1146 838 310 146
EcoICRI GAG'CTC
Cuts at: 987 1337 987
Size: 350 3975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 532 728 2150 2582 3329 3597 184
Size: 348 196 1422 432 747 268 912
Fragments arranged by size:
1422 912 747 432 348 268 196
EcoNI CCTnn'n_nnAGG
Cuts at: 907 907
Size: 4325
EcoO109I rG'GnC_Cy
Cuts at: 1464 1464
Size: 4325
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 417 562 1047 2344 417
Size: 145 485 1297 2398
Fragments arranged by size:
2398 1297 485 145
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4325
HaeII r_GCGC'y
Cuts at: 1759 2007 3029 4008 1759
Size: 248 1022 979 2076
Fragments arranged by size:
2076 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 224 256 798 830 2167 2197 2199 2229 224
Size: 32 542 32 1337 30 2 30 2320
Fragments arranged by size:
2320 1337 542 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1364 1364
Size: 4325
KasI G'GCGC_C
Cuts at: 1755 2003 1755
Size: 248 4077
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4325
MluI A'CGCG_T
Cuts at: 3758 3758
Size: 4325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2996 3180 2996
Size: 184 4141
MscI TGG'CCA
Cuts at: 2086 2086
Size: 4325
MslI CAynn'nnrTG
Cuts at: 821 2314 2441 821
Size: 1493 127 2705
Fragments arranged by size:
2705 1493 127
MspA1I CmG'CkG
Cuts at: 115 552 1436 1750 2110 3123 3368 115
Size: 437 884 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 884 437 360 314 245
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 4325
NarI GG'CG_CC
Cuts at: 1756 2004 1756
Size: 248 4077
NcoI C'CATG_G
Cuts at: 107 107
Size: 4325
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 4325
NspI r_CATG'y
Cuts at: 1005 1267 1378 1878 2785 1005
Size: 262 111 500 907 2545
Fragments arranged by size:
2545 907 500 262 111
PciI A'CATG_T
Cuts at: 2781 2781
Size: 4325
PfoI T'CCnGG_A
Cuts at: 602 602
Size: 4325
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1264 1296 3464 3496 1264
Size: 32 2168 32 2093
Fragments arranged by size:
2168 2093 32 32
PstI C_TGCA'G
Cuts at: 134 1372 2057 134
Size: 1238 685 2402
Fragments arranged by size:
2402 1238 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4325
PvuII CAG'CTG
Cuts at: 1750 2110 1750
Size: 360 3965
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4325
SacI G_AGCT'C
Cuts at: 989 1339 989
Size: 350 3975
SacII CC_GC'GG
Cuts at: 116 116
Size: 4325
SalI G'TCGA_C
Cuts at: 1362 1362
Size: 4325
SapI GCTCTTCn'nnn_
Cuts at: 872 2348 2558 872
Size: 1476 210 2639
Fragments arranged by size:
2639 1476 210
SbfI CC_TGCA'GG
Cuts at: 1372 1372
Size: 4325
ScaI AGT'ACT
Cuts at: 3842 3842
Size: 4325
SfcI C'TryA_G
Cuts at: 33 130 1368 2053 3046 3237 33
Size: 97 1238 685 993 191 1121
Fragments arranged by size:
1238 1121 993 685 191 97
SfoI GGC'GCC
Cuts at: 1757 2005 1757
Size: 248 4077
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
SmaI CCC'GGG
Cuts at: 1347 1347
Size: 4325
SmlI C'TyrA_G
Cuts at: 413 1480 2395 2887 3149 413
Size: 1067 915 492 262 1589
Fragments arranged by size:
1589 1067 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4325
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3825
StyI C'CwwG_G
Cuts at: 107 1459 1813 107
Size: 1352 354 2619
Fragments arranged by size:
2619 1352 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1908 2041 2500 2709 1908
Size: 133 459 209 3524
Fragments arranged by size:
3524 459 209 133
TatI w'GTAC_w
Cuts at: 3840 3840
Size: 4325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4293
Tth111I GACn'n_nGTC
Cuts at: 238 525 2122 238
Size: 287 1597 2441
Fragments arranged by size:
2441 1597 287
XbaI T'CTAG_A
Cuts at: 1356 1356
Size: 4325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1820 1820
Size: 4325
XhoI C'TCGA_G
Cuts at: 413 413
Size: 4325
XmaI C'CCGG_G
Cuts at: 1345 1345
Size: 4325
XmnI GAAnn'nnTTC
Cuts at: 542 748 542
Size: 206 4119
ZraI GAC'GTC
Cuts at: 1113 1113
Size: 4325
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI