Restriction map for
pF1KB8158
(Circular) (Six-base) MAPSORT of: pF1KB8158.seq Check: 1520 from: 1 to: 4244
pF1KB8158 4244 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AatII G_ACGT'C
Cuts at: 1035 1035
Size: 4244
AccI GT'mk_AC
Cuts at: 311 323 743 911 1282 311
Size: 12 420 168 371 3273
Fragments arranged by size:
3273 420 371 168 12
Acc65I G'GTAC_C
Cuts at: 533 1260 533
Size: 727 3517
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 2069 2501 3248 3516 664
Size: 1405 432 747 268 1392
Fragments arranged by size:
1405 1392 747 432 268
AfeI AGC'GCT
Cuts at: 220 220
Size: 4244
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AlwNI CAG_nnn'CTG
Cuts at: 626 1225 1549 3116 626
Size: 599 324 1567 1754
Fragments arranged by size:
1754 1567 599 324
ApaI G_GGCC'C
Cuts at: 139 139
Size: 4244
ApaLI G'TGCA_C
Cuts at: 3014 3014
Size: 4244
ApoI r'AATT_y
Cuts at: 1248 3597 3853 1248
Size: 2349 256 1639
Fragments arranged by size:
2349 1639 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 1139 1264 1139
Size: 125 4119
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 182 533 952 1260 1674 1922 1957 182
Size: 351 419 308 414 248 35 2469
Fragments arranged by size:
2469 419 414 351 308 248 35
BanII G_rGCy'C
Cuts at: 139 1196 1258 2288 139
Size: 1057 62 1030 2095
Fragments arranged by size:
2095 1057 1030 62
BbeI G_GCGC'C
Cuts at: 186 1678 1926 186
Size: 1492 248 2504
Fragments arranged by size:
2504 1492 248
BbsI GAAGACnn'nnnn_
Cuts at: 489 489
Size: 4244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4210
BciVI GTATCCnnnnn_n'
Cuts at: 1110 2142 2903 4244 1110
Size: 1032 761 1341 1110
Fragments arranged by size:
1341 1110 1032 761
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 1356 1356
Size: 4244
Bme1580I G_kGCm'C
Cuts at: 139 957 1869 1962 3018 139
Size: 818 912 93 1056 1365
Fragments arranged by size:
1365 1056 912 818 93
BmrI ACTGGGnnnn_n'
Cuts at: 1456 1537 1872 4016 1456
Size: 81 335 2144 1684
Fragments arranged by size:
2144 1684 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 2299 2791 3089 1420
Size: 879 492 298 2575
Fragments arranged by size:
2575 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3948 3948
Size: 4244
BsaAI yAC'GTr
Cuts at: 1597 1597
Size: 4244
BsaBI GATnn'nnATC
Cuts at: 6 1758 3795 6
Size: 1752 2037 455
Fragments arranged by size:
2037 1752 455
BsaHI Gr'CG_yC
Cuts at: 183 1032 1675 1923 2625 4121 183
Size: 849 643 248 702 1496 306
Fragments arranged by size:
1496 849 702 643 306 248
BsaWI w'CCGG_w
Cuts at: 1433 1954 2632 2906 3053 3464 1433
Size: 521 678 274 147 411 2213
Fragments arranged by size:
2213 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 185 215 185
Size: 30 4214
BseYI C'CCAG_C
Cuts at: 132 171 1187 1669 2427 3004 3500 132
Size: 39 1016 482 758 577 496 876
Fragments arranged by size:
1016 876 758 577 496 482 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 948
Size: 4244
BsiEI CG_ry'CG
Cuts at: 104 1786 1832 3040 104
Size: 1682 46 1208 1308
Fragments arranged by size:
1682 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1148 1258 2036 2226 3018 1148
Size: 110 778 190 792 2374
Fragments arranged by size:
2374 792 778 190 110
BsmBI CGTCTCn'nnnn_
Cuts at: 573 1023 573
Size: 450 3794
Bsp1286I G_dGCh'C
Cuts at: 139 957 1148 1196 1258 1869 1962 2036 2226
Size: 818 191 48 62 611 93 74 190
Cuts at: 2226 2288 3018 139
Size: 62 730 1365
Fragments arranged by size:
1365 818 730 611 191 190 93 74
62 62 48
BspEI T'CCGG_A
Cuts at: 3464 3464
Size: 4244
BspHI T'CATG_A
Cuts at: 725 3420 725
Size: 2695 1549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1294 1508 1810 2191 1294
Size: 214 302 381 3347
Fragments arranged by size:
3347 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2156 2156
Size: 4244
BsrFI r'CCGG_y
Cuts at: 2242 2423 2632 2242
Size: 181 209 3854
Fragments arranged by size:
3854 209 181
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 239 626 1621 239
Size: 387 995 2862
Fragments arranged by size:
2862 995 387
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 1177 1177
Size: 4244
BstYI r'GATC_y
Cuts at: 1 1269 1736 1759 2094 2340 3352 3438 3450
Size: 1268 467 23 335 246 1012 86 12
Cuts at: 3450 3796 1
Size: 346 449
Fragments arranged by size:
1268 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1170 1170
Size: 4244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 282 615 657 1241 1487 282
Size: 333 42 584 246 3039
Fragments arranged by size:
3039 584 333 246 42
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 1829 2003 2394 2421 3766 1829
Size: 174 391 27 1345 2307
Fragments arranged by size:
2307 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 515 2452 2762 2908 4054 145
Size: 370 1937 310 146 1146 335
Fragments arranged by size:
1937 1146 370 335 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 2069 2501 3248 3516 664
Size: 1405 432 747 268 1392
Fragments arranged by size:
1405 1392 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 765 1383 765
Size: 618 3626
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
EcoRV GAT'ATC
Cuts at: 558 558
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 4244
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 186 222 228 1678 1926 2948 3927 186
Size: 36 6 1450 248 1022 979 503
Fragments arranged by size:
1450 1022 979 503 248 36 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2086 2116 2118 2148 2086
Size: 30 2 30 4182
Fragments arranged by size:
4182 30 30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
KasI G'GCGC_C
Cuts at: 182 1674 1922 182
Size: 1492 248 2504
Fragments arranged by size:
2504 1492 248
KpnI G_GTAC'C
Cuts at: 537 1264 537
Size: 727 3517
MfeI C'AATT_G
Cuts at: 675 675
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3099 2915
Size: 184 4060
MscI TGG'CCA
Cuts at: 2005 2005
Size: 4244
MslI CAynn'nnrTG
Cuts at: 1175 2233 2360 1175
Size: 1058 127 3059
Fragments arranged by size:
3059 1058 127
MspA1I CmG'CkG
Cuts at: 120 188 286 338 1355 1669 2029 3042 3287
Size: 68 98 52 1017 314 360 1013 245
Cuts at: 3287 120
Size: 1077
Fragments arranged by size:
1077 1017 1013 360 314 245 98 68
52
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 4244
NarI GG'CG_CC
Cuts at: 183 1675 1923 183
Size: 1492 248 2504
Fragments arranged by size:
2504 1492 248
NcoI C'CATG_G
Cuts at: 1170 1170
Size: 4244
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 4244
NspI r_CATG'y
Cuts at: 973 1297 1797 2704 973
Size: 324 500 907 2513
Fragments arranged by size:
2513 907 500 324
PasI CC'CwG_GG
Cuts at: 543 948 1202 543
Size: 405 254 3585
Fragments arranged by size:
3585 405 254
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PfoI T'CCnGG_A
Cuts at: 773 773
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 4212
PpuMI rG'GwC_Cy
Cuts at: 765 765
Size: 4244
PspOMI G'GGCC_C
Cuts at: 135 135
Size: 4244
PstI C_TGCA'G
Cuts at: 622 751 1291 1976 622
Size: 129 540 685 2890
Fragments arranged by size:
2890 685 540 129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 1669 2029 1669
Size: 360 3884
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 468 618 747 826 1039 1287 1972 2965
Size: 435 150 129 79 213 248 685 993
Cuts at: 2965 3156 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 435 248 213 191 150
129 79
SfoI GGC'GCC
Cuts at: 184 1676 1924 184
Size: 1492 248 2504
Fragments arranged by size:
2504 1492 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 1399 2314 2806 3068 1399
Size: 915 492 262 2575
Fragments arranged by size:
2575 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 973 1297 1797 973
Size: 324 500 3420
Fragments arranged by size:
3420 500 324
StyI C'CwwG_G
Cuts at: 1046 1170 1378 1732 1046
Size: 124 208 354 3558
Fragments arranged by size:
3558 354 208 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 882 940 1099 1194 1827 1960 2419 2628 882
Size: 58 159 95 633 133 459 209 2498
Fragments arranged by size:
2498 633 459 209 159 133 95 58
TatI w'GTAC_w
Cuts at: 213 3759 213
Size: 3546 698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 185 217 3761 3793 185
Size: 32 3544 32 636
Fragments arranged by size:
3544 636 32 32
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 452 1739 452
Size: 1287 2957
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
XmnI GAAnn'nnTTC
Cuts at: 1102 1102
Size: 4244
ZraI GAC'GTC
Cuts at: 1033 1033
Size: 4244
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI Bpu10I
BseRI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI