Restriction map for
pF1KB8165
(Circular) (Six-base) MAPSORT of: pF1KB8165.seq Check: 1922 from: 1 to: 4601
pF1KB8165 4601 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 644 1639 644
Size: 995 3606
Acc65I G'GTAC_C
Cuts at: 628 1085 1617 628
Size: 457 532 3612
Fragments arranged by size:
3612 532 457
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 868 2426 2858 3605 3873 347
Size: 521 1558 432 747 268 1075
Fragments arranged by size:
1558 1075 747 521 432 268
AflIII A'CryG_T
Cuts at: 253 1139 1953 3057 4034 253
Size: 886 814 1104 977 820
Fragments arranged by size:
1104 977 886 820 814
AgeI A'CCGG_T
Cuts at: 2989 2989
Size: 4601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1576 1608 1576
Size: 32 4569
AlwNI CAG_nnn'CTG
Cuts at: 858 981 1906 3473 858
Size: 123 925 1567 1986
Fragments arranged by size:
1986 1567 925 123
ApaI G_GGCC'C
Cuts at: 292 292
Size: 4601
ApaLI G'TGCA_C
Cuts at: 316 3371 316
Size: 3055 1546
ApoI r'AATT_y
Cuts at: 1605 3954 4210 1605
Size: 2349 256 1996
Fragments arranged by size:
2349 1996 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 1031 1621 1031
Size: 590 4011
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1069 1102 1069
Size: 33 4568
BamHI G'GATC_C
Cuts at: 1463 1626 1463
Size: 163 4438
BanI G'GyrC_C
Cuts at: 184 628 717 1085 1617 2031 2279 2314 184
Size: 444 89 368 532 414 248 35 2471
Fragments arranged by size:
2471 532 444 414 368 248 89 35
BanII G_rGCy'C
Cuts at: 292 1007 1201 1615 2645 292
Size: 715 194 414 1030 2248
Fragments arranged by size:
2248 1030 715 414 194
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BbsI GAAGACnn'nnnn_
Cuts at: 1532 1532
Size: 4601
BbvCI CC'TCA_GC
Cuts at: 541 541
Size: 4601
BciVI GTATCCnnnnn_n'
Cuts at: 2499 3260 4601 2499
Size: 761 1341 2499
Fragments arranged by size:
2499 1341 761
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BglII A'GATC_T
Cuts at: 431 2116 4153 431
Size: 1685 2037 879
Fragments arranged by size:
2037 1685 879
BlpI GC'TnA_GC
Cuts at: 1713 1713
Size: 4601
Bme1580I G_kGCm'C
Cuts at: 189 292 320 720 2226 2319 3375 189
Size: 103 28 400 1506 93 1056 1415
Fragments arranged by size:
1506 1415 1056 400 103 93 28
BmgBI CAC'GTC
Cuts at: 371 1262 371
Size: 891 3710
BmrI ACTGGGnnnn_n'
Cuts at: 628 1813 1894 2229 4373 628
Size: 1185 81 335 2144 856
Fragments arranged by size:
2144 1185 856 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 377 520 613 260
Size: 117 143 93 4248
Fragments arranged by size:
4248 143 117 93
Bpu10I CC'TnA_GC
Cuts at: 191 541 191
Size: 350 4251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 736 952 991 1777 2656 3148 3446 670
Size: 66 216 39 786 879 492 298 1825
Fragments arranged by size:
1825 879 786 492 298 216 66 39
BsaI GGTCTCn'nnnn_
Cuts at: 589 956 4305 589
Size: 367 3349 885
Fragments arranged by size:
3349 885 367
BsaAI yAC'GTr
Cuts at: 256 1954 256
Size: 1698 2903
BsaBI GATnn'nnATC
Cuts at: 6 2115 4152 6
Size: 2109 2037 455
Fragments arranged by size:
2109 2037 455
BsaHI Gr'CG_yC
Cuts at: 2032 2280 2982 4478 2032
Size: 248 702 1496 2155
Fragments arranged by size:
2155 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1790 2311 2989 3263 3410 3821 1790
Size: 521 678 274 147 411 2570
Fragments arranged by size:
2570 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1270 1300 1363 1270
Size: 30 63 4508
Fragments arranged by size:
4508 63 30
BseYI C'CCAG_C
Cuts at: 2026 2784 3361 3857 2026
Size: 758 577 496 2770
Fragments arranged by size:
2770 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 287 406 708 266
Size: 21 119 302 4159
Fragments arranged by size:
4159 302 119 21
BsiEI CG_ry'CG
Cuts at: 104 2143 2189 3397 104
Size: 2039 46 1208 1308
Fragments arranged by size:
2039 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 320 1007 1615 2393 2583 3375 320
Size: 687 608 778 190 792 1546
Fragments arranged by size:
1546 792 778 687 608 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1252 1252
Size: 4601
Bsp1286I G_dGCh'C
Cuts at: 189 292 320 720 1007 1201 1615 2226 2319
Size: 103 28 400 287 194 414 611 93
Cuts at: 2319 2393 2583 2645 3375 189
Size: 74 190 62 730 1415
Fragments arranged by size:
1415 730 611 414 400 287 194 190
103 93 74 62 28
BspEI T'CCGG_A
Cuts at: 3821 3821
Size: 4601
BspHI T'CATG_A
Cuts at: 3777 3777
Size: 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 989 1651 1865 2167 2548 989
Size: 662 214 302 381 3042
Fragments arranged by size:
3042 662 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1461 2893 2947 4431 4596 50
Size: 1411 1432 54 1484 165 55
Fragments arranged by size:
1484 1432 1411 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2513 2513
Size: 4601
BsrFI r'CCGG_y
Cuts at: 941 1088 2599 2780 2989 941
Size: 147 1511 181 209 2553
Fragments arranged by size:
2553 1511 209 181 147
BssSI C'ACGA_G
Cuts at: 319 2872 3230 319
Size: 2553 358 1690
Fragments arranged by size:
2553 1690 358
BstAPI GCAn_nnn'nTGC
Cuts at: 951 1423 1978 951
Size: 472 555 3574
Fragments arranged by size:
3574 555 472
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 1071 1071
Size: 4601
BstYI r'GATC_y
Cuts at: 1 431 1409 1463 1626 2093 2116 2451 2697
Size: 430 978 54 163 467 23 335 246
Cuts at: 2697 3709 3795 3807 4153 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 978 467 449 430 346 335 246
163 86 54 23 12
Bsu36I CC'TnA_GG
Cuts at: 488 488
Size: 4601
BtgI C'CryG_G
Cuts at: 107 921 1115 1202 107
Size: 814 194 87 3506
Fragments arranged by size:
3506 814 194 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2551 2732 2895 2551
Size: 181 163 4257
Fragments arranged by size:
4257 181 163
BtsI GCAGTG_nn'
Cuts at: 237 1844 237
Size: 1607 2994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1116 1151 1116
Size: 35 4566
DraI TTT'AAA
Cuts at: 1600 1600
Size: 4601
DraIII CAC_nnn'GTG
Cuts at: 894 894
Size: 4601
DrdI GACnn_nn'nnGTC
Cuts at: 2307 3165 2307
Size: 858 3743
EaeI y'GGCC_r
Cuts at: 159 1172 1264 2186 2360 2751 2778 4123 159
Size: 1013 92 922 174 391 27 1345 637
Fragments arranged by size:
1345 1013 922 637 391 174 92 27
EagI C'GGCC_G
Cuts at: 2186 2186
Size: 4601
EarI CTCTTCn'nnn_
Cuts at: 704 2624 2834 704
Size: 1920 210 2471
Fragments arranged by size:
2471 1920 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2809 3119 3265 4411 2809
Size: 310 146 1146 2999
Fragments arranged by size:
2999 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1005 1613 1005
Size: 608 3993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 347 377 520 613 868 2426 2858 3605
Size: 87 30 143 93 255 1558 432 747
Cuts at: 3605 3873 260
Size: 268 988
Fragments arranged by size:
1558 988 747 432 268 255 143 93
87 30
EcoNI CCTnn'n_nnAGG
Cuts at: 751 838 1556 751
Size: 87 718 3796
Fragments arranged by size:
3796 718 87
EcoO109I rG'GnC_Cy
Cuts at: 246 288 756 843 1326 1740 246
Size: 42 468 87 483 414 3107
Fragments arranged by size:
3107 483 468 414 87 42
EcoRI G'AATT_C
Cuts at: 1605 1605
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4601
FspI TGC'GCA
Cuts at: 412 2382 412
Size: 1970 2631
HaeII r_GCGC'y
Cuts at: 459 2035 2283 3305 4284 459
Size: 1576 248 1022 979 776
Fragments arranged by size:
1576 1022 979 776 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 2443 2473 2475 2505 373
Size: 32 2038 30 2 30 2469
Fragments arranged by size:
2469 2038 32 30 30 2
HincII GTy'rAC
Cuts at: 1640 1640
Size: 4601
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 632 1089 1621 632
Size: 457 532 3612
Fragments arranged by size:
3612 532 457
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3272 3456 3272
Size: 184 4417
MscI TGG'CCA
Cuts at: 1174 1266 2362 1174
Size: 92 1096 3413
Fragments arranged by size:
3413 1096 92
MslI CAynn'nnrTG
Cuts at: 883 2590 2717 883
Size: 1707 127 2767
Fragments arranged by size:
2767 1707 127
MspA1I CmG'CkG
Cuts at: 138 472 915 1712 2026 2386 3399 3644 138
Size: 334 443 797 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 797 443 360 334 314 245
NaeI GCC'GGC
Cuts at: 2782 2782
Size: 4601
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NcoI C'CATG_G
Cuts at: 107 107
Size: 4601
NgoMIV G'CCGG_C
Cuts at: 2780 2780
Size: 4601
NspI r_CATG'y
Cuts at: 888 1143 1654 2154 3061 888
Size: 255 511 500 907 2428
Fragments arranged by size:
2428 907 511 500 255
PciI A'CATG_T
Cuts at: 1139 3057 1139
Size: 1918 2683
PfoI T'CCnGG_A
Cuts at: 734 734
Size: 4601
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PmlI CAC'GTG
Cuts at: 256 256
Size: 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4569
PpuMI rG'GwC_Cy
Cuts at: 756 843 756
Size: 87 4514
PspOMI G'GGCC_C
Cuts at: 288 288
Size: 4601
PstI C_TGCA'G
Cuts at: 1648 2333 1648
Size: 685 3916
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4601
PvuII CAG'CTG
Cuts at: 138 472 915 2026 2386 138
Size: 334 443 1111 360 2353
Fragments arranged by size:
2353 1111 443 360 334
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 1007 1615 1007
Size: 608 3993
SalI G'TCGA_C
Cuts at: 1638 1638
Size: 4601
SapI GCTCTTCn'nnn_
Cuts at: 2624 2834 2624
Size: 210 4391
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SfcI C'TryA_G
Cuts at: 33 1644 2329 3322 3513 33
Size: 1611 685 993 191 1121
Fragments arranged by size:
1611 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 1623 1623
Size: 4601
SmlI C'TyrA_G
Cuts at: 685 751 967 1006 1756 2671 3163 3425 685
Size: 66 216 39 750 915 492 262 1861
Fragments arranged by size:
1861 915 750 492 262 216 66 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
StuI AGG'CCT
Cuts at: 203 203
Size: 4601
StyI C'CwwG_G
Cuts at: 107 1735 2089 107
Size: 1628 354 2619
Fragments arranged by size:
2619 1628 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1526 2184 2317 2776 2985 1526
Size: 658 133 459 209 3142
Fragments arranged by size:
3142 658 459 209 133
TatI w'GTAC_w
Cuts at: 4116 4116
Size: 4601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1167 1199 4118 4150 1167
Size: 32 2919 32 1618
Fragments arranged by size:
2919 1618 32 32
Tth111I GACn'n_nGTC
Cuts at: 2398 2398
Size: 4601
XbaI T'CTAG_A
Cuts at: 1632 1632
Size: 4601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 919 2096 919
Size: 1177 3424
XmaI C'CCGG_G
Cuts at: 1621 1621
Size: 4601
XmnI GAAnn'nnTTC
Cuts at: 901 901
Size: 4601
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BcgI BfrBI BglI BmtI BsaXI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI