Restriction map for
pF1KB8168
(Circular) (Six-base) MAPSORT of: pF1KB8168.seq Check: 374 from: 1 to: 4025
pF1KB8168 4025 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 1063 1063
Size: 4025
Acc65I G'GTAC_C
Cuts at: 333 1041 333
Size: 708 3317
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1850 2282 3029 3297 935
Size: 915 432 747 268 1663
Fragments arranged by size:
1663 915 747 432 268
AflIII A'CryG_T
Cuts at: 1377 2481 3458 1377
Size: 1104 977 1944
Fragments arranged by size:
1944 1104 977
AgeI A'CCGG_T
Cuts at: 822 2413 822
Size: 1591 2434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 377 476 377
Size: 99 3926
AlwNI CAG_nnn'CTG
Cuts at: 1330 2897 1330
Size: 1567 2458
ApaLI G'TGCA_C
Cuts at: 2795 2795
Size: 4025
ApoI r'AATT_y
Cuts at: 1029 3378 3634 1029
Size: 2349 256 1420
Fragments arranged by size:
2349 1420 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 124 1045 124
Size: 921 3104
BamHI G'GATC_C
Cuts at: 1050 1050
Size: 4025
BanI G'GyrC_C
Cuts at: 333 616 721 783 1041 1455 1703 1738 333
Size: 283 105 62 258 414 248 35 2620
Fragments arranged by size:
2620 414 283 258 248 105 62 35
BanII G_rGCy'C
Cuts at: 120 769 949 1039 2069 120
Size: 649 180 90 1030 2076
Fragments arranged by size:
2076 1030 649 180 90
BbeI G_GCGC'C
Cuts at: 620 787 1459 1707 620
Size: 167 672 248 2938
Fragments arranged by size:
2938 672 248 167
BbsI GAAGACnn'nnnn_
Cuts at: 648 888 648
Size: 240 3785
BciVI GTATCCnnnnn_n'
Cuts at: 1923 2684 4025 1923
Size: 761 1341 1923
Fragments arranged by size:
1923 1341 761
BclI T'GATC_A
Cuts at: 1545 1545
Size: 4025
BglII A'GATC_T
Cuts at: 1540 3577 1540
Size: 2037 1988
BlpI GC'TnA_GC
Cuts at: 365 1137 365
Size: 772 3253
Bme1580I G_kGCm'C
Cuts at: 724 1650 1743 2799 724
Size: 926 93 1056 1950
Fragments arranged by size:
1950 1056 926 93
BmrI ACTGGGnnnn_n'
Cuts at: 332 1237 1318 1653 3797 332
Size: 905 81 335 2144 560
Fragments arranged by size:
2144 905 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 150 227 2392 150
Size: 77 2165 1783
Fragments arranged by size:
2165 1783 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 1201 2080 2572 2870 222
Size: 979 879 492 298 1377
Fragments arranged by size:
1377 979 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 151 287 3729 151
Size: 136 3442 447
Fragments arranged by size:
3442 447 136
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 1539 3576 6
Size: 1533 2037 455
Fragments arranged by size:
2037 1533 455
BsaHI Gr'CG_yC
Cuts at: 617 784 1456 1704 2406 3902 617
Size: 167 672 248 702 1496 740
Fragments arranged by size:
1496 740 702 672 248 167
BsaWI w'CCGG_w
Cuts at: 296 777 822 1214 1735 2413 2687 2834 3245
Size: 481 45 392 521 678 274 147 411
Cuts at: 3245 296
Size: 1076
Fragments arranged by size:
1076 678 521 481 411 392 274 147
45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 812 842 1004 1034 812
Size: 30 162 30 3803
Fragments arranged by size:
3803 162 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 864 126
Size: 738 3287
BseYI C'CCAG_C
Cuts at: 596 762 1450 2208 2785 3281 596
Size: 166 688 758 577 496 1340
Fragments arranged by size:
1340 758 688 577 496 166
BsiEI CG_ry'CG
Cuts at: 104 1567 1613 2821 104
Size: 1463 46 1208 1308
Fragments arranged by size:
1463 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 120 830 1039 1817 2007 2799 120
Size: 710 209 778 190 792 1346
Fragments arranged by size:
1346 792 778 710 209 190
BsmI GAATG_Cn'
Cuts at: 499 499
Size: 4025
Bsp1286I G_dGCh'C
Cuts at: 120 724 769 830 949 1039 1650 1743 1817
Size: 604 45 61 119 90 611 93 74
Cuts at: 1817 2007 2069 2799 120
Size: 190 62 730 1346
Fragments arranged by size:
1346 730 611 604 190 119 93 90
74 62 61 45
BspEI T'CCGG_A
Cuts at: 777 3245 777
Size: 2468 1557
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1075 1289 1591 1972 1075
Size: 214 302 381 3128
Fragments arranged by size:
3128 381 302 214
BsrBI CCG'CTC
Cuts at: 50 614 2317 2371 3855 4020 50
Size: 564 1703 54 1484 165 55
Fragments arranged by size:
1703 1484 564 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1937 1937
Size: 4025
BsrFI r'CCGG_y
Cuts at: 822 2023 2204 2413 822
Size: 1201 181 209 2434
Fragments arranged by size:
2434 1201 209 181
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 508 1402 508
Size: 894 3131
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstEII G'GTnAC_C
Cuts at: 837 837
Size: 4025
BstXI CCAn_nnnn'nTGG
Cuts at: 168 168
Size: 4025
BstYI r'GATC_y
Cuts at: 1 358 383 1050 1517 1540 1875 2121 3133
Size: 357 25 667 467 23 335 246 1012
Cuts at: 3133 3219 3231 3577 1
Size: 86 12 346 449
Fragments arranged by size:
1012 667 467 449 357 346 335 246
86 25 23 12
BtgI C'CryG_G
Cuts at: 398 708 725 737 398
Size: 310 17 12 3686
Fragments arranged by size:
3686 310 17 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 660 1975 2156 2319 660
Size: 1315 181 163 2366
Fragments arranged by size:
2366 1315 181 163
BtsI GCAGTG_nn'
Cuts at: 1268 1268
Size: 4025
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 251 705 1610 1784 2175 2202 3547 251
Size: 454 905 174 391 27 1345 729
Fragments arranged by size:
1345 905 729 454 391 174 27
EagI C'GGCC_G
Cuts at: 1610 1610
Size: 4025
EarI CTCTTCn'nnn_
Cuts at: 2048 2258 2048
Size: 210 3815
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 834 2233 2543 2689 3835 834
Size: 1399 310 146 1146 1024
Fragments arranged by size:
1399 1146 1024 310 146
EcoICRI GAG'CTC
Cuts at: 118 1037 118
Size: 919 3106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1850 2282 3029 3297 935
Size: 915 432 747 268 1663
Fragments arranged by size:
1663 915 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1164 1164
Size: 4025
EcoRI G'AATT_C
Cuts at: 1029 1029
Size: 4025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 4025
FspI TGC'GCA
Cuts at: 1806 1806
Size: 4025
HaeII r_GCGC'y
Cuts at: 288 620 787 802 1459 1707 2729 3708 288
Size: 332 167 15 657 248 1022 979 605
Fragments arranged by size:
1022 979 657 605 332 248 167 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 284 316 387 419 812 844 1867 1897 1899
Size: 32 71 32 393 32 1023 30 2
Cuts at: 1899 1929 284
Size: 30 2380
Fragments arranged by size:
2380 1023 393 71 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1064 1064
Size: 4025
KasI G'GCGC_C
Cuts at: 616 783 1455 1703 616
Size: 167 672 248 2938
Fragments arranged by size:
2938 672 248 167
KpnI G_GTAC'C
Cuts at: 337 1045 337
Size: 708 3317
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2696 2880 2696
Size: 184 3841
MscI TGG'CCA
Cuts at: 253 1786 253
Size: 1533 2492
MslI CAynn'nnrTG
Cuts at: 166 2014 2141 166
Size: 1848 127 2050
Fragments arranged by size:
2050 1848 127
MspA1I CmG'CkG
Cuts at: 600 687 1136 1450 1810 2823 3068 600
Size: 87 449 314 360 1013 245 1557
Fragments arranged by size:
1557 1013 449 360 314 245 87
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 617 784 1456 1704 617
Size: 167 672 248 2938
Fragments arranged by size:
2938 672 248 167
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NspI r_CATG'y
Cuts at: 1078 1578 2485 1078
Size: 500 907 2618
Fragments arranged by size:
2618 907 500
PasI CC'CwG_GG
Cuts at: 644 644
Size: 4025
PciI A'CATG_T
Cuts at: 2481 2481
Size: 4025
PflMI CCAn_nnn'nTGG
Cuts at: 249 249
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PstI C_TGCA'G
Cuts at: 392 1072 1757 392
Size: 680 685 2660
Fragments arranged by size:
2660 685 680
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 1450 1810 1450
Size: 360 3665
RsrII CG'GwC_CG
Cuts at: 820 2220 820
Size: 1400 2625
SacI G_AGCT'C
Cuts at: 120 1039 120
Size: 919 3106
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 2048 2258 2048
Size: 210 3815
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SfcI C'TryA_G
Cuts at: 33 388 987 1068 1753 2746 2937 33
Size: 355 599 81 685 993 191 1121
Fragments arranged by size:
1121 993 685 599 355 191 81
SfoI GGC'GCC
Cuts at: 618 785 1457 1705 618
Size: 167 672 248 2938
Fragments arranged by size:
2938 672 248 167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 1047 1047
Size: 4025
SmlI C'TyrA_G
Cuts at: 201 1180 2095 2587 2849 201
Size: 979 915 492 262 1377
Fragments arranged by size:
1377 979 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 1078 1578 1078
Size: 500 3525
StyI C'CwwG_G
Cuts at: 975 994 1159 1513 975
Size: 19 165 354 3487
Fragments arranged by size:
3487 354 165 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 750 882 972 1608 1741 2200 2409 750
Size: 132 90 636 133 459 209 2366
Fragments arranged by size:
2366 636 459 209 133 132 90
TatI w'GTAC_w
Cuts at: 128 3540 128
Size: 3412 613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 3993
Tth111I GACn'n_nGTC
Cuts at: 1822 1822
Size: 4025
XbaI T'CTAG_A
Cuts at: 1056 1056
Size: 4025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1520 1520
Size: 4025
XmaI C'CCGG_G
Cuts at: 1045 1045
Size: 4025
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI