Restriction map for
pF1KB8169
(Circular) (Six-base) MAPSORT of: pF1KB8169.seq Check: 6914 from: 1 to: 4109
pF1KB8169 4109 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 159 159
Size: 4109
AccI GT'mk_AC
Cuts at: 962 1147 962
Size: 185 3924
Acc65I G'GTAC_C
Cuts at: 1125 1125
Size: 4109
AclI AA'CG_TT
Cuts at: 3949 3949
Size: 4109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1934 2366 3113 3381 1934
Size: 432 747 268 2662
Fragments arranged by size:
2662 747 432 268
AflIII A'CryG_T
Cuts at: 1461 2565 3542 1461
Size: 1104 977 2028
Fragments arranged by size:
2028 1104 977
AgeI A'CCGG_T
Cuts at: 2497 2497
Size: 4109
AhdI GACnn_n'nnGTC
Cuts at: 696 696
Size: 4109
AleI CACnn'nnGTG
Cuts at: 614 614
Size: 4109
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 529 561 529
Size: 32 4077
AlwNI CAG_nnn'CTG
Cuts at: 225 1414 2981 225
Size: 1189 1567 1353
Fragments arranged by size:
1567 1353 1189
ApaI G_GGCC'C
Cuts at: 299 1019 299
Size: 720 3389
ApaLI G'TGCA_C
Cuts at: 2879 2879
Size: 4109
ApoI r'AATT_y
Cuts at: 133 1113 3462 3718 133
Size: 980 2349 256 524
Fragments arranged by size:
2349 980 524 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4109
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
AvaI C'yCGr_G
Cuts at: 594 1129 594
Size: 535 3574
BamHI G'GATC_C
Cuts at: 406 833 1134 406
Size: 427 301 3381
Fragments arranged by size:
3381 427 301
BanI G'GyrC_C
Cuts at: 1049 1125 1539 1787 1822 1049
Size: 76 414 248 35 3336
Fragments arranged by size:
3336 414 248 76 35
BanII G_rGCy'C
Cuts at: 299 383 428 664 1019 1123 2153 299
Size: 84 45 236 355 104 1030 2255
Fragments arranged by size:
2255 1030 355 236 104 84 45
BbeI G_GCGC'C
Cuts at: 1543 1791 1543
Size: 248 3861
BbsI GAAGACnn'nnnn_
Cuts at: 400 400
Size: 4109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4075
BciVI GTATCCnnnnn_n'
Cuts at: 2007 2768 4109 2007
Size: 761 1341 2007
Fragments arranged by size:
2007 1341 761
BclI T'GATC_A
Cuts at: 1629 1629
Size: 4109
BglI GCCn_nnn'nGGC
Cuts at: 117 387 986 117
Size: 270 599 3240
Fragments arranged by size:
3240 599 270
BglII A'GATC_T
Cuts at: 1624 3661 1624
Size: 2037 2072
BlpI GC'TnA_GC
Cuts at: 746 1221 746
Size: 475 3634
Bme1580I G_kGCm'C
Cuts at: 299 982 1019 1054 1734 1827 2883 299
Size: 683 37 35 680 93 1056 1525
Fragments arranged by size:
1525 1056 683 680 93 37 35
BmrI ACTGGGnnnn_n'
Cuts at: 664 1062 1321 1402 1737 3881 664
Size: 398 259 81 335 2144 892
Fragments arranged by size:
2144 892 398 335 259 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 531 600 217
Size: 314 69 3726
Fragments arranged by size:
3726 314 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 2164 2656 2954 1285
Size: 879 492 298 2440
Fragments arranged by size:
2440 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 153 3813 153
Size: 3660 449
BsaAI yAC'GTr
Cuts at: 897 1462 897
Size: 565 3544
BsaBI GATnn'nnATC
Cuts at: 6 1623 3660 6
Size: 1617 2037 455
Fragments arranged by size:
2037 1617 455
BsaHI Gr'CG_yC
Cuts at: 470 1540 1788 2490 3986 470
Size: 1070 248 702 1496 593
Fragments arranged by size:
1496 1070 702 593 248
BsaWI w'CCGG_w
Cuts at: 1298 1819 2497 2771 2918 3329 1298
Size: 521 678 274 147 411 2078
Fragments arranged by size:
2078 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 529 559 529
Size: 30 4079
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1093 1093
Size: 4109
BseYI C'CCAG_C
Cuts at: 382 487 829 1534 2292 2869 3365 382
Size: 105 342 705 758 577 496 1126
Fragments arranged by size:
1126 758 705 577 496 342 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 592 361
Size: 231 3878
BsiEI CG_ry'CG
Cuts at: 104 1651 1697 2905 104
Size: 1547 46 1208 1308
Fragments arranged by size:
1547 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 428 664 1123 1901 2091 2883 428
Size: 236 459 778 190 792 1654
Fragments arranged by size:
1654 792 778 459 236 190
BsmBI CGTCTCn'nnnn_
Cuts at: 461 461
Size: 4109
Bsp1286I G_dGCh'C
Cuts at: 299 383 428 664 982 1019 1054 1123 1734
Size: 84 45 236 318 37 35 69 611
Cuts at: 1734 1827 1901 2091 2153 2883 299
Size: 93 74 190 62 730 1525
Fragments arranged by size:
1525 730 611 318 236 190 93 84
74 69 62 45 37 35
BspEI T'CCGG_A
Cuts at: 3329 3329
Size: 4109
BspHI T'CATG_A
Cuts at: 998 3285 998
Size: 2287 1822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 159 360 1159 1373 1675 2056 159
Size: 201 799 214 302 381 2212
Fragments arranged by size:
2212 799 381 302 214 201
BsrBI CCG'CTC
Cuts at: 50 2401 2455 3939 4104 50
Size: 2351 54 1484 165 55
Fragments arranged by size:
2351 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2021 2021
Size: 4109
BsrFI r'CCGG_y
Cuts at: 2107 2288 2497 2107
Size: 181 209 3719
Fragments arranged by size:
3719 209 181
BsrGI T'GTAC_A
Cuts at: 821 821
Size: 4109
BssSI C'ACGA_G
Cuts at: 2380 2738 2380
Size: 358 3751
BstAPI GCAn_nnn'nTGC
Cuts at: 484 1486 484
Size: 1002 3107
BstBI TT'CG_AA
Cuts at: 2470 2470
Size: 4109
BstXI CCAn_nnnn'nTGG
Cuts at: 250 250
Size: 4109
BstYI r'GATC_y
Cuts at: 1 406 833 1134 1601 1624 1959 2205 3217
Size: 405 427 301 467 23 335 246 1012
Cuts at: 3217 3303 3315 3661 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 427 405 346 335 301
246 86 23 12
BtgI C'CryG_G
Cuts at: 249 249
Size: 4109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2059 2240 2403 2059
Size: 181 163 3765
Fragments arranged by size:
3765 181 163
BtsI GCAGTG_nn'
Cuts at: 763 1063 1096 1352 763
Size: 300 33 256 3520
Fragments arranged by size:
3520 300 256 33
DraI TTT'AAA
Cuts at: 1108 1108
Size: 4109
DraIII CAC_nnn'GTG
Cuts at: 900 1046 900
Size: 146 3963
DrdI GACnn_nn'nnGTC
Cuts at: 244 1815 2673 244
Size: 1571 858 1680
Fragments arranged by size:
1680 1571 858
EaeI y'GGCC_r
Cuts at: 987 1694 1868 2259 2286 3631 987
Size: 707 174 391 27 1345 1465
Fragments arranged by size:
1465 1345 707 391 174 27
EagI C'GGCC_G
Cuts at: 1694 1694
Size: 4109
EarI CTCTTCn'nnn_
Cuts at: 669 2132 2342 669
Size: 1463 210 2436
Fragments arranged by size:
2436 1463 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2317 2627 2773 3919 2317
Size: 310 146 1146 2507
Fragments arranged by size:
2507 1146 310 146
EcoICRI GAG'CTC
Cuts at: 426 662 1121 426
Size: 236 459 3414
Fragments arranged by size:
3414 459 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 531 600 1934 2366 3113 3381 217
Size: 314 69 1334 432 747 268 945
Fragments arranged by size:
1334 945 747 432 314 268 69
EcoO109I rG'GnC_Cy
Cuts at: 295 296 585 882 1248 295
Size: 1 289 297 366 3156
Fragments arranged by size:
3156 366 297 289 1
EcoRI G'AATT_C
Cuts at: 1113 1113
Size: 4109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 4109
FspI TGC'GCA
Cuts at: 1890 1890
Size: 4109
HaeII r_GCGC'y
Cuts at: 1543 1791 2813 3792 1543
Size: 248 1022 979 1860
Fragments arranged by size:
1860 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 394 426 682 714 818 850 1951 1981 1983
Size: 32 256 32 104 32 1101 30 2
Cuts at: 1983 2013 394
Size: 30 2490
Fragments arranged by size:
2490 1101 256 104 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1148 1148
Size: 4109
KasI G'GCGC_C
Cuts at: 1539 1787 1539
Size: 248 3861
KpnI G_GTAC'C
Cuts at: 1129 1129
Size: 4109
MluI A'CGCG_T
Cuts at: 3542 3542
Size: 4109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2780 2964 2780
Size: 184 3925
MscI TGG'CCA
Cuts at: 989 1870 989
Size: 881 3228
MslI CAynn'nnrTG
Cuts at: 614 2098 2225 614
Size: 1484 127 2498
Fragments arranged by size:
2498 1484 127
MspA1I CmG'CkG
Cuts at: 386 1220 1534 1894 2907 3152 386
Size: 834 314 360 1013 245 1343
Fragments arranged by size:
1343 1013 834 360 314 245
NaeI GCC'GGC
Cuts at: 2290 2290
Size: 4109
NarI GG'CG_CC
Cuts at: 1540 1788 1540
Size: 248 3861
NcoI C'CATG_G
Cuts at: 249 249
Size: 4109
NgoMIV G'CCGG_C
Cuts at: 2288 2288
Size: 4109
NspI r_CATG'y
Cuts at: 1162 1662 2569 1162
Size: 500 907 2702
Fragments arranged by size:
2702 907 500
PasI CC'CwG_GG
Cuts at: 435 435
Size: 4109
PciI A'CATG_T
Cuts at: 2565 2565
Size: 4109
PfoI T'CCnGG_A
Cuts at: 233 233
Size: 4109
PmeI GTTT'AAAC
Cuts at: 1108 1108
Size: 4109
PmlI CAC'GTG
Cuts at: 897 897
Size: 4109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 529 561 3248 3280 529
Size: 32 2687 32 1358
Fragments arranged by size:
2687 1358 32 32
PpuMI rG'GwC_Cy
Cuts at: 585 882 585
Size: 297 3812
PsiI TTA'TAA
Cuts at: 641 641
Size: 4109
PspOMI G'GGCC_C
Cuts at: 295 1015 295
Size: 720 3389
PstI C_TGCA'G
Cuts at: 221 423 480 926 1156 1841 221
Size: 202 57 446 230 685 2489
Fragments arranged by size:
2489 685 446 230 202 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4109
PvuII CAG'CTG
Cuts at: 1534 1894 1534
Size: 360 3749
RsrII CG'GwC_CG
Cuts at: 2304 2304
Size: 4109
SacI G_AGCT'C
Cuts at: 428 664 1123 428
Size: 236 459 3414
Fragments arranged by size:
3414 459 236
SalI G'TCGA_C
Cuts at: 1146 1146
Size: 4109
SanDI GG'GwC_CC
Cuts at: 585 585
Size: 4109
SapI GCTCTTCn'nnn_
Cuts at: 669 2132 2342 669
Size: 1463 210 2436
Fragments arranged by size:
2436 1463 210
SbfI CC_TGCA'GG
Cuts at: 480 1156 480
Size: 676 3433
ScaI AGT'ACT
Cuts at: 3626 3626
Size: 4109
SexAI A'CCwGG_T
Cuts at: 992 1042 992
Size: 50 4059
SfcI C'TryA_G
Cuts at: 33 202 217 419 476 922 1152 1837 2830
Size: 169 15 202 57 446 230 685 993
Cuts at: 2830 3021 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 446 230 202 191 169
57 15
SfoI GGC'GCC
Cuts at: 1541 1789 1541
Size: 248 3861
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
SmaI CCC'GGG
Cuts at: 1131 1131
Size: 4109
SmlI C'TyrA_G
Cuts at: 1264 2179 2671 2933 1264
Size: 915 492 262 2440
Fragments arranged by size:
2440 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4109
SphI G_CATG'C
Cuts at: 1162 1662 1162
Size: 500 3609
StyI C'CwwG_G
Cuts at: 249 936 1243 1597 249
Size: 687 307 354 2761
Fragments arranged by size:
2761 687 354 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 522 696 1692 1825 2284 2493 256
Size: 266 174 996 133 459 209 1872
Fragments arranged by size:
1872 996 459 266 209 174 133
TatI w'GTAC_w
Cuts at: 821 910 3624 821
Size: 89 2714 1306
Fragments arranged by size:
2714 1306 89
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4077
Tth111I GACn'n_nGTC
Cuts at: 1906 1906
Size: 4109
XbaI T'CTAG_A
Cuts at: 279 1140 279
Size: 861 3248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1604 1604
Size: 4109
XmaI C'CCGG_G
Cuts at: 1129 1129
Size: 4109
XmnI GAAnn'nnTTC
Cuts at: 262 541 262
Size: 279 3830
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AscI AvrII BaeI BbvCI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PshAI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI