Restriction map for
pF1KB8172
(Circular) (Six-base) MAPSORT of: pF1KB8172.seq Check: 5284 from: 1 to: 3533
pF1KB8172 3533 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 571 571
Size: 3533
Acc65I G'GTAC_C
Cuts at: 549 549
Size: 3533
AclI AA'CG_TT
Cuts at: 3373 3373
Size: 3533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1358 1790 2537 2805 542
Size: 816 432 747 268 1270
Fragments arranged by size:
1270 816 747 432 268
AflIII A'CryG_T
Cuts at: 885 1989 2966 885
Size: 1104 977 1452
Fragments arranged by size:
1452 1104 977
AgeI A'CCGG_T
Cuts at: 1921 1921
Size: 3533
AlwNI CAG_nnn'CTG
Cuts at: 274 382 838 2405 274
Size: 108 456 1567 1402
Fragments arranged by size:
1567 1402 456 108
ApaLI G'TGCA_C
Cuts at: 2303 2303
Size: 3533
ApoI r'AATT_y
Cuts at: 440 537 2886 3142 440
Size: 97 2349 256 831
Fragments arranged by size:
2349 831 256 97
AseI AT'TA_AT
Cuts at: 20 20
Size: 3533
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
AvaI C'yCGr_G
Cuts at: 553 553
Size: 3533
BamHI G'GATC_C
Cuts at: 462 558 462
Size: 96 3437
BanI G'GyrC_C
Cuts at: 549 963 1211 1246 549
Size: 414 248 35 2836
Fragments arranged by size:
2836 414 248 35
BanII G_rGCy'C
Cuts at: 429 547 1577 429
Size: 118 1030 2385
Fragments arranged by size:
2385 1030 118
BbeI G_GCGC'C
Cuts at: 967 1215 967
Size: 248 3285
BbsI GAAGACnn'nnnn_
Cuts at: 286 352 286
Size: 66 3467
BciVI GTATCCnnnnn_n'
Cuts at: 1431 2192 3533 1431
Size: 761 1341 1431
Fragments arranged by size:
1431 1341 761
BclI T'GATC_A
Cuts at: 1053 1053
Size: 3533
BglII A'GATC_T
Cuts at: 1048 3085 1048
Size: 2037 1496
BlpI GC'TnA_GC
Cuts at: 645 645
Size: 3533
Bme1580I G_kGCm'C
Cuts at: 1158 1251 2307 1158
Size: 93 1056 2384
Fragments arranged by size:
2384 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 745 826 1161 3305 745
Size: 81 335 2144 973
Fragments arranged by size:
2144 973 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1588 2080 2378 709
Size: 879 492 298 1864
Fragments arranged by size:
1864 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3237 3237
Size: 3533
BsaAI yAC'GTr
Cuts at: 886 886
Size: 3533
BsaBI GATnn'nnATC
Cuts at: 6 1047 3084 6
Size: 1041 2037 455
Fragments arranged by size:
2037 1041 455
BsaHI Gr'CG_yC
Cuts at: 964 1212 1914 3410 964
Size: 248 702 1496 1087
Fragments arranged by size:
1496 1087 702 248
BsaWI w'CCGG_w
Cuts at: 722 1243 1921 2195 2342 2753 722
Size: 521 678 274 147 411 1502
Fragments arranged by size:
1502 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 299 302 329 332 299
Size: 3 27 3 3500
Fragments arranged by size:
3500 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 323 323
Size: 3533
BseYI C'CCAG_C
Cuts at: 958 1716 2293 2789 958
Size: 758 577 496 1702
Fragments arranged by size:
1702 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1075 1121 2329 104
Size: 971 46 1208 1308
Fragments arranged by size:
1308 1208 971 46
BsiHKAI G_wGCw'C
Cuts at: 547 1325 1515 2307 547
Size: 778 190 792 1773
Fragments arranged by size:
1773 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 429 547 1158 1251 1325 1515 1577 2307 429
Size: 118 611 93 74 190 62 730 1655
Fragments arranged by size:
1655 730 611 190 118 93 74 62
BspEI T'CCGG_A
Cuts at: 2753 2753
Size: 3533
BspHI T'CATG_A
Cuts at: 119 2709 119
Size: 2590 943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 583 797 1099 1480 583
Size: 214 302 381 2636
Fragments arranged by size:
2636 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1825 1879 3363 3528 50
Size: 1775 54 1484 165 55
Fragments arranged by size:
1775 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 368 1445 368
Size: 1077 2456
BsrFI r'CCGG_y
Cuts at: 1531 1712 1921 1531
Size: 181 209 3143
Fragments arranged by size:
3143 209 181
BssSI C'ACGA_G
Cuts at: 1804 2162 1804
Size: 358 3175
BstAPI GCAn_nnn'nTGC
Cuts at: 910 910
Size: 3533
BstBI TT'CG_AA
Cuts at: 1894 1894
Size: 3533
BstYI r'GATC_y
Cuts at: 1 462 558 1025 1048 1383 1629 2641 2727
Size: 461 96 467 23 335 246 1012 86
Cuts at: 2727 2739 3085 1
Size: 12 346 449
Fragments arranged by size:
1012 467 461 449 346 335 246 96
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1483 1664 1827 1483
Size: 181 163 3189
Fragments arranged by size:
3189 181 163
BtsI GCAGTG_nn'
Cuts at: 320 776 320
Size: 456 3077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 369 404 369
Size: 35 3498
DraI TTT'AAA
Cuts at: 532 532
Size: 3533
DrdI GACnn_nn'nnGTC
Cuts at: 1239 2097 1239
Size: 858 2675
EaeI y'GGCC_r
Cuts at: 1118 1292 1683 1710 3055 1118
Size: 174 391 27 1345 1596
Fragments arranged by size:
1596 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1118 1118
Size: 3533
EarI CTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1741 2051 2197 3343 1741
Size: 310 146 1146 1931
Fragments arranged by size:
1931 1146 310 146
EcoICRI GAG'CTC
Cuts at: 545 545
Size: 3533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1358 1790 2537 2805 542
Size: 816 432 747 268 1270
Fragments arranged by size:
1270 816 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 672 672
Size: 3533
EcoRI G'AATT_C
Cuts at: 440 537 440
Size: 97 3436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1552 1552
Size: 3533
FspI TGC'GCA
Cuts at: 1314 1314
Size: 3533
HaeII r_GCGC'y
Cuts at: 967 1215 2237 3216 967
Size: 248 1022 979 1284
Fragments arranged by size:
1284 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1375 1405 1407 1437 1375
Size: 30 2 30 3471
Fragments arranged by size:
3471 30 30 2
HincII GTy'rAC
Cuts at: 572 572
Size: 3533
KasI G'GCGC_C
Cuts at: 963 1211 963
Size: 248 3285
KpnI G_GTAC'C
Cuts at: 553 553
Size: 3533
MluI A'CGCG_T
Cuts at: 2966 2966
Size: 3533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 285 2204 2388 285
Size: 1919 184 1430
Fragments arranged by size:
1919 1430 184
MscI TGG'CCA
Cuts at: 1294 1294
Size: 3533
MslI CAynn'nnrTG
Cuts at: 1522 1649 1522
Size: 127 3406
MspA1I CmG'CkG
Cuts at: 644 958 1318 2331 2576 644
Size: 314 360 1013 245 1601
Fragments arranged by size:
1601 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1714 1714
Size: 3533
NarI GG'CG_CC
Cuts at: 964 1212 964
Size: 248 3285
NcoI C'CATG_G
Cuts at: 107 107
Size: 3533
NgoMIV G'CCGG_C
Cuts at: 1712 1712
Size: 3533
NspI r_CATG'y
Cuts at: 586 1086 1993 586
Size: 500 907 2126
Fragments arranged by size:
2126 907 500
PciI A'CATG_T
Cuts at: 1989 1989
Size: 3533
PmeI GTTT'AAAC
Cuts at: 532 532
Size: 3533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2672 2704 2672
Size: 32 3501
PstI C_TGCA'G
Cuts at: 580 1265 580
Size: 685 2848
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3533
PvuII CAG'CTG
Cuts at: 958 1318 958
Size: 360 3173
RsrII CG'GwC_CG
Cuts at: 1728 1728
Size: 3533
SacI G_AGCT'C
Cuts at: 547 547
Size: 3533
SalI G'TCGA_C
Cuts at: 570 570
Size: 3533
SapI GCTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
SbfI CC_TGCA'GG
Cuts at: 580 580
Size: 3533
ScaI AGT'ACT
Cuts at: 3050 3050
Size: 3533
SfcI C'TryA_G
Cuts at: 33 576 1261 2254 2445 33
Size: 543 685 993 191 1121
Fragments arranged by size:
1121 993 685 543 191
SfoI GGC'GCC
Cuts at: 965 1213 965
Size: 248 3285
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
SmaI CCC'GGG
Cuts at: 555 555
Size: 3533
SmlI C'TyrA_G
Cuts at: 688 1603 2095 2357 688
Size: 915 492 262 1864
Fragments arranged by size:
1864 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3533
SphI G_CATG'C
Cuts at: 586 1086 586
Size: 500 3033
StyI C'CwwG_G
Cuts at: 107 140 239 667 1021 107
Size: 33 99 428 354 2619
Fragments arranged by size:
2619 428 354 99 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1116 1249 1708 1917 1116
Size: 133 459 209 2732
Fragments arranged by size:
2732 459 209 133
TatI w'GTAC_w
Cuts at: 3048 3048
Size: 3533
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 302 331 334 3050 3082 299
Size: 3 29 3 2716 32 750
Fragments arranged by size:
2716 750 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 1330 1330
Size: 3533
XbaI T'CTAG_A
Cuts at: 564 564
Size: 3533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 180 246 1028 180
Size: 66 782 2685
Fragments arranged by size:
2685 782 66
XmaI C'CCGG_G
Cuts at: 553 553
Size: 3533
XmnI GAAnn'nnTTC
Cuts at: 440 440
Size: 3533
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI