Restriction map for
pF1KB8173
(Circular) (Six-base) MAPSORT of: pF1KB8173.seq Check: 7179 from: 1 to: 4187
pF1KB8173 4187 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 545 545
Size: 4187
AatII G_ACGT'C
Cuts at: 404 404
Size: 4187
AccI GT'mk_AC
Cuts at: 1225 1225
Size: 4187
Acc65I G'GTAC_C
Cuts at: 1203 1203
Size: 4187
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1050 2012 2444 3191 3459 286
Size: 764 962 432 747 268 1014
Fragments arranged by size:
1014 962 764 747 432 268
AflIII A'CryG_T
Cuts at: 1113 1539 2643 3620 1113
Size: 426 1104 977 1680
Fragments arranged by size:
1680 1104 977 426
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AhdI GACnn_n'nnGTC
Cuts at: 710 710
Size: 4187
AleI CACnn'nnGTG
Cuts at: 784 784
Size: 4187
AlwNI CAG_nnn'CTG
Cuts at: 237 525 1492 3059 237
Size: 288 967 1567 1365
Fragments arranged by size:
1567 1365 967 288
ApaI G_GGCC'C
Cuts at: 209 209
Size: 4187
ApaLI G'TGCA_C
Cuts at: 2957 2957
Size: 4187
ApoI r'AATT_y
Cuts at: 1191 3540 3796 1191
Size: 2349 256 1582
Fragments arranged by size:
2349 1582 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 1207 1207
Size: 4187
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 1203 1617 1865 1900 1203
Size: 414 248 35 3490
Fragments arranged by size:
3490 414 248 35
BanII G_rGCy'C
Cuts at: 209 968 1201 2231 209
Size: 759 233 1030 2165
Fragments arranged by size:
2165 1030 759 233
BbeI G_GCGC'C
Cuts at: 1621 1869 1621
Size: 248 3939
BciVI GTATCCnnnnn_n'
Cuts at: 2085 2846 4187 2085
Size: 761 1341 2085
Fragments arranged by size:
2085 1341 761
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BglI GCCn_nnn'nGGC
Cuts at: 1076 1076
Size: 4187
BglII A'GATC_T
Cuts at: 284 1702 3739 284
Size: 1418 2037 732
Fragments arranged by size:
2037 1418 732
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 194 209 1812 1905 2961 194
Size: 15 1603 93 1056 1420
Fragments arranged by size:
1603 1420 1056 93 15
BmrI ACTGGGnnnn_n'
Cuts at: 791 1399 1480 1815 3959 791
Size: 608 81 335 2144 1019
Fragments arranged by size:
2144 1019 608 335 81
BmtI G_CTAG'C
Cuts at: 176 176
Size: 4187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 562 568 738 413
Size: 149 6 170 3862
Fragments arranged by size:
3862 170 149 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1363 2242 2734 3032 1363
Size: 879 492 298 2518
Fragments arranged by size:
2518 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 665 3891 665
Size: 3226 961
BsaAI yAC'GTr
Cuts at: 1116 1540 1116
Size: 424 3763
BsaBI GATnn'nnATC
Cuts at: 6 565 1701 3738 6
Size: 559 1136 2037 455
Fragments arranged by size:
2037 1136 559 455
BsaHI Gr'CG_yC
Cuts at: 401 1618 1866 2568 4064 401
Size: 1217 248 702 1496 524
Fragments arranged by size:
1496 1217 702 524 248
BsaWI w'CCGG_w
Cuts at: 1376 1897 2575 2849 2996 3407 1376
Size: 521 678 274 147 411 2156
Fragments arranged by size:
2156 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 336 366 386 391 416 421 613 643 336
Size: 30 20 5 25 5 192 30 3880
Fragments arranged by size:
3880 192 30 30 25 20 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 399 468 946 399
Size: 69 478 3640
Fragments arranged by size:
3640 478 69
BseYI C'CCAG_C
Cuts at: 230 373 378 624 637 1612 2370 2947 3443
Size: 143 5 246 13 975 758 577 496
Cuts at: 3443 230
Size: 974
Fragments arranged by size:
975 974 758 577 496 246 143 13
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1079 1079
Size: 4187
BsiEI CG_ry'CG
Cuts at: 104 1729 1775 2983 104
Size: 1625 46 1208 1308
Fragments arranged by size:
1625 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1201 1979 2169 2961 1201
Size: 778 190 792 2427
Fragments arranged by size:
2427 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 761 1002 1096 761
Size: 241 94 3852
Fragments arranged by size:
3852 241 94
Bsp1286I G_dGCh'C
Cuts at: 194 209 968 1201 1812 1905 1979 2169 2231
Size: 15 759 233 611 93 74 190 62
Cuts at: 2231 2961 194
Size: 730 1420
Fragments arranged by size:
1420 759 730 611 233 190 93 74
62 15
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 441 545 1237 1451 1753 2134 441
Size: 104 692 214 302 381 2494
Fragments arranged by size:
2494 692 381 302 214 104
BsrBI CCG'CTC
Cuts at: 50 300 2479 2533 4017 4182 50
Size: 250 2179 54 1484 165 55
Fragments arranged by size:
2179 1484 250 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 221 2185 2366 2575 221
Size: 1964 181 209 1833
Fragments arranged by size:
1964 1833 209 181
BsrGI T'GTAC_A
Cuts at: 693 693
Size: 4187
BssSI C'ACGA_G
Cuts at: 2458 2816 2458
Size: 358 3829
BstAPI GCAn_nnn'nTGC
Cuts at: 1564 1564
Size: 4187
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstXI CCAn_nnnn'nTGG
Cuts at: 573 573
Size: 4187
BstYI r'GATC_y
Cuts at: 1 284 857 1212 1679 1702 2037 2283 3295
Size: 283 573 355 467 23 335 246 1012
Cuts at: 3295 3381 3393 3739 1
Size: 86 12 346 449
Fragments arranged by size:
1012 573 467 449 355 346 335 283
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 417 2137 2318 2481 417
Size: 1720 181 163 2123
Fragments arranged by size:
2123 1720 181 163
BtsI GCAGTG_nn'
Cuts at: 1430 1430
Size: 4187
ClaI AT'CG_AT
Cuts at: 1031 1031
Size: 4187
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 2751 1893
Size: 858 3329
EaeI y'GGCC_r
Cuts at: 872 1118 1772 1946 2337 2364 3709 872
Size: 246 654 174 391 27 1345 1350
Fragments arranged by size:
1350 1345 654 391 246 174 27
EagI C'GGCC_G
Cuts at: 1772 1772
Size: 4187
EarI CTCTTCn'nnn_
Cuts at: 262 307 478 844 2210 2420 262
Size: 45 171 366 1366 210 2029
Fragments arranged by size:
2029 1366 366 210 171 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2395 2705 2851 3997 2395
Size: 310 146 1146 2585
Fragments arranged by size:
2585 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1199 1199
Size: 4187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 413 562 568 738 1050 2012 2444 3191
Size: 127 149 6 170 312 962 432 747
Cuts at: 3191 3459 286
Size: 268 1014
Fragments arranged by size:
1014 962 747 432 312 268 170 149
127 6
EcoO109I rG'GnC_Cy
Cuts at: 205 206 467 704 726 1326 205
Size: 1 261 237 22 600 3066
Fragments arranged by size:
3066 600 261 237 22 1
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 4187
FspI TGC'GCA
Cuts at: 1968 1968
Size: 4187
HaeII r_GCGC'y
Cuts at: 1621 1869 2891 3870 1621
Size: 248 1022 979 1938
Fragments arranged by size:
1938 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 284 316 386 391 418 423 673 696 705
Size: 32 70 5 27 5 250 23 9
Cuts at: 705 728 2029 2059 2061 2091 284
Size: 23 1301 30 2 30 2380
Fragments arranged by size:
2380 1301 250 70 32 30 30 27
23 23 9 5 5 2
HincII GTy'rAC
Cuts at: 984 1226 984
Size: 242 3945
KasI G'GCGC_C
Cuts at: 1617 1865 1617
Size: 248 3939
KpnI G_GTAC'C
Cuts at: 1207 1207
Size: 4187
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 506 1064 2858 3042 506
Size: 558 1794 184 1651
Fragments arranged by size:
1794 1651 558 184
MscI TGG'CCA
Cuts at: 874 1948 874
Size: 1074 3113
MslI CAynn'nnrTG
Cuts at: 520 784 2176 2303 520
Size: 264 1392 127 2404
Fragments arranged by size:
2404 1392 264 127
MspA1I CmG'CkG
Cuts at: 948 1298 1612 1972 2985 3230 948
Size: 350 314 360 1013 245 1905
Fragments arranged by size:
1905 1013 360 350 314 245
NaeI GCC'GGC
Cuts at: 2368 2368
Size: 4187
NarI GG'CG_CC
Cuts at: 1618 1866 1618
Size: 248 3939
NcoI C'CATG_G
Cuts at: 107 107
Size: 4187
NgoMIV G'CCGG_C
Cuts at: 2366 2366
Size: 4187
NheI G'CTAG_C
Cuts at: 172 172
Size: 4187
NspI r_CATG'y
Cuts at: 350 1240 1740 2647 350
Size: 890 500 907 1890
Fragments arranged by size:
1890 907 890 500
PciI A'CATG_T
Cuts at: 2643 2643
Size: 4187
PflMI CCAn_nnn'nTGG
Cuts at: 540 721 540
Size: 181 4006
PfoI T'CCnGG_A
Cuts at: 1131 1131
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PmlI CAC'GTG
Cuts at: 1116 1116
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 4155
PpuMI rG'GwC_Cy
Cuts at: 704 704
Size: 4187
PspOMI G'GGCC_C
Cuts at: 205 205
Size: 4187
PstI C_TGCA'G
Cuts at: 604 1234 1919 604
Size: 630 685 2872
Fragments arranged by size:
2872 685 630
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 948 1612 1972 948
Size: 664 360 3163
Fragments arranged by size:
3163 664 360
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 1201 1201
Size: 4187
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SanDI GG'GwC_CC
Cuts at: 704 704
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 307 2210 2420 307
Size: 1903 210 2074
Fragments arranged by size:
2074 1903 210
SbfI CC_TGCA'GG
Cuts at: 1234 1234
Size: 4187
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SexAI A'CCwGG_T
Cuts at: 572 572
Size: 4187
SfcI C'TryA_G
Cuts at: 33 600 1230 1915 2908 3099 33
Size: 567 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 567 191
SfoI GGC'GCC
Cuts at: 1619 1867 1619
Size: 248 3939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 1209 1209
Size: 4187
SmlI C'TyrA_G
Cuts at: 1342 2257 2749 3011 1342
Size: 915 492 262 2518
Fragments arranged by size:
2518 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4187
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
StyI C'CwwG_G
Cuts at: 107 762 866 1321 1675 107
Size: 655 104 455 354 2619
Fragments arranged by size:
2619 655 455 354 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1770 1903 2362 2571 1770
Size: 133 459 209 3386
Fragments arranged by size:
3386 459 209 133
TatI w'GTAC_w
Cuts at: 693 3702 693
Size: 3009 1178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 4155
Tth111I GACn'n_nGTC
Cuts at: 574 1984 574
Size: 1410 2777
XbaI T'CTAG_A
Cuts at: 1218 1218
Size: 4187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 682 726 1682 489
Size: 193 44 956 2994
Fragments arranged by size:
2994 956 193 44
XmaI C'CCGG_G
Cuts at: 1207 1207
Size: 4187
ZraI GAC'GTC
Cuts at: 402 402
Size: 4187
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmgBI Bpu10I BsiWI BsmI BssHII
BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PasI PshAI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI