Restriction map for
pF1KB8174
(Circular) (Six-base) MAPSORT of: pF1KB8174.seq Check: 3632 from: 1 to: 4802
pF1KB8174 4802 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1840 1840
Size: 4802
Acc65I G'GTAC_C
Cuts at: 153 1818 153
Size: 1665 3137
AclI AA'CG_TT
Cuts at: 4642 4642
Size: 4802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 206 523 1694 2627 3059 3806 4074 182
Size: 24 317 1171 933 432 747 268 910
Fragments arranged by size:
1171 933 910 747 432 317 268 24
AflIII A'CryG_T
Cuts at: 2154 3258 4235 2154
Size: 1104 977 2721
Fragments arranged by size:
2721 1104 977
AgeI A'CCGG_T
Cuts at: 3190 3190
Size: 4802
AleI CACnn'nnGTG
Cuts at: 1312 1312
Size: 4802
AlwNI CAG_nnn'CTG
Cuts at: 979 1672 2107 3674 979
Size: 693 435 1567 2107
Fragments arranged by size:
2107 1567 693 435
ApaLI G'TGCA_C
Cuts at: 3572 3572
Size: 4802
ApoI r'AATT_y
Cuts at: 622 830 1806 4155 4411 622
Size: 208 976 2349 256 1013
Fragments arranged by size:
2349 1013 976 256 208
AseI AT'TA_AT
Cuts at: 20 20
Size: 4802
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4802
AvaI C'yCGr_G
Cuts at: 1822 1822
Size: 4802
BamHI G'GATC_C
Cuts at: 1827 1827
Size: 4802
BanI G'GyrC_C
Cuts at: 153 1818 2232 2480 2515 153
Size: 1665 414 248 35 2440
Fragments arranged by size:
2440 1665 414 248 35
BanII G_rGCy'C
Cuts at: 654 1255 1369 1504 1816 2846 654
Size: 601 114 135 312 1030 2610
Fragments arranged by size:
2610 1030 601 312 135 114
BbeI G_GCGC'C
Cuts at: 2236 2484 2236
Size: 248 4554
BbsI GAAGACnn'nnnn_
Cuts at: 194 194
Size: 4802
BbvCI CC'TCA_GC
Cuts at: 1357 1406 1714 1357
Size: 49 308 4445
Fragments arranged by size:
4445 308 49
BciVI GTATCCnnnnn_n'
Cuts at: 2700 3461 4802 2700
Size: 761 1341 2700
Fragments arranged by size:
2700 1341 761
BclI T'GATC_A
Cuts at: 450 458 635 2322 450
Size: 8 177 1687 2930
Fragments arranged by size:
2930 1687 177 8
BglII A'GATC_T
Cuts at: 2317 4354 2317
Size: 2037 2765
BlpI GC'TnA_GC
Cuts at: 1498 1914 1498
Size: 416 4386
Bme1580I G_kGCm'C
Cuts at: 2427 2520 3576 2427
Size: 93 1056 3653
Fragments arranged by size:
3653 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 127 1306 1540 2014 2095 2430 4574 127
Size: 1179 234 474 81 335 2144 355
Fragments arranged by size:
2144 1179 474 355 335 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1274 3169 1274
Size: 1895 2907
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 1207 1353 638
Size: 569 146 4087
Fragments arranged by size:
4087 569 146
Bpu10I CC'TnA_GC
Cuts at: 544 1199 1357 1406 1714 544
Size: 655 158 49 308 3632
Fragments arranged by size:
3632 655 308 158 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1269 1978 2857 3349 3647 1269
Size: 709 879 492 298 2424
Fragments arranged by size:
2424 879 709 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1437 4506 1437
Size: 3069 1733
BsaAI yAC'GTr
Cuts at: 2155 2155
Size: 4802
BsaBI GATnn'nnATC
Cuts at: 6 724 2316 4353 6
Size: 718 1592 2037 455
Fragments arranged by size:
2037 1592 718 455
BsaHI Gr'CG_yC
Cuts at: 2233 2481 3183 4679 2233
Size: 248 702 1496 2356
Fragments arranged by size:
2356 1496 702 248
BsaWI w'CCGG_w
Cuts at: 562 1991 2512 3190 3464 3611 4022 562
Size: 1429 521 678 274 147 411 1342
Fragments arranged by size:
1429 1342 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 817 934 1132 1250 1425 1738 385
Size: 432 117 198 118 175 313 3449
Fragments arranged by size:
3449 432 313 198 175 118 117
BseYI C'CCAG_C
Cuts at: 247 2227 2985 3562 4058 247
Size: 1980 758 577 496 991
Fragments arranged by size:
1980 991 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 703
Size: 4802
BsiEI CG_ry'CG
Cuts at: 104 2344 2390 3598 104
Size: 2240 46 1208 1308
Fragments arranged by size:
2240 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 654 1195 1504 1816 2594 2784 3576 654
Size: 541 309 312 778 190 792 1880
Fragments arranged by size:
1880 792 778 541 312 309 190
Bsp1286I G_dGCh'C
Cuts at: 654 1195 1255 1369 1504 1816 2427 2520 2594
Size: 541 60 114 135 312 611 93 74
Cuts at: 2594 2784 2846 3576 654
Size: 190 62 730 1880
Fragments arranged by size:
1880 730 611 541 312 190 135 114
93 74 62 60
BspEI T'CCGG_A
Cuts at: 562 4022 562
Size: 3460 1342
BspHI T'CATG_A
Cuts at: 3978 3978
Size: 4802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 616 1852 2066 2368 2749 616
Size: 1236 214 302 381 2669
Fragments arranged by size:
2669 1236 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3094 3148 4632 4797 50
Size: 3044 54 1484 165 55
Fragments arranged by size:
3044 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2714 2714
Size: 4802
BsrFI r'CCGG_y
Cuts at: 2800 2981 3190 2800
Size: 181 209 4412
Fragments arranged by size:
4412 209 181
BssSI C'ACGA_G
Cuts at: 3073 3431 3073
Size: 358 4444
BstAPI GCAn_nnn'nTGC
Cuts at: 2179 2179
Size: 4802
BstBI TT'CG_AA
Cuts at: 3163 3163
Size: 4802
BstYI r'GATC_y
Cuts at: 1 1827 2294 2317 2652 2898 3910 3996 4008
Size: 1826 467 23 335 246 1012 86 12
Cuts at: 4008 4354 1
Size: 346 449
Fragments arranged by size:
1826 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 791 797 1331 1547 1574 107
Size: 684 6 534 216 27 3335
Fragments arranged by size:
3335 684 534 216 27 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2752 2933 3096 2752
Size: 181 163 4458
Fragments arranged by size:
4458 181 163
BtsI GCAGTG_nn'
Cuts at: 648 1390 2045 648
Size: 742 655 3405
Fragments arranged by size:
3405 742 655
DraI TTT'AAA
Cuts at: 1801 1801
Size: 4802
DraIII CAC_nnn'GTG
Cuts at: 1773 1773
Size: 4802
DrdI GACnn_nn'nnGTC
Cuts at: 2508 3366 2508
Size: 858 3944
EaeI y'GGCC_r
Cuts at: 794 1228 1349 1571 2387 2561 2952 2979 4324
Size: 434 121 222 816 174 391 27 1345
Cuts at: 4324 794
Size: 1272
Fragments arranged by size:
1345 1272 816 434 391 222 174 121
27
EagI C'GGCC_G
Cuts at: 2387 2387
Size: 4802
EarI CTCTTCn'nnn_
Cuts at: 508 728 740 881 1417 2825 3035 508
Size: 220 12 141 536 1408 210 2275
Fragments arranged by size:
2275 1408 536 220 210 141 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3010 3320 3466 4612 3010
Size: 310 146 1146 3200
Fragments arranged by size:
3200 1146 310 146
EcoICRI GAG'CTC
Cuts at: 652 1502 1814 652
Size: 850 312 3640
Fragments arranged by size:
3640 850 312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 206 523 638 1207 1353 1694 2627 3059
Size: 24 317 115 569 146 341 933 432
Cuts at: 3059 3806 4074 182
Size: 747 268 910
Fragments arranged by size:
933 910 747 569 432 341 317 268
146 115 24
EcoNI CCTnn'n_nnAGG
Cuts at: 161 1360 161
Size: 1199 3603
EcoO109I rG'GnC_Cy
Cuts at: 1214 1941 1214
Size: 727 4075
EcoRI G'AATT_C
Cuts at: 1806 1806
Size: 4802
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4802
FspI TGC'GCA
Cuts at: 2583 2583
Size: 4802
HaeII r_GCGC'y
Cuts at: 1495 2236 2484 3506 4485 1495
Size: 741 248 1022 979 1812
Fragments arranged by size:
1812 1022 979 741 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2644 2674 2676 2706 2644
Size: 30 2 30 4740
Fragments arranged by size:
4740 30 30 2
HincII GTy'rAC
Cuts at: 1841 1841
Size: 4802
KasI G'GCGC_C
Cuts at: 2232 2480 2232
Size: 248 4554
KpnI G_GTAC'C
Cuts at: 157 1822 157
Size: 1665 3137
MluI A'CGCG_T
Cuts at: 4235 4235
Size: 4802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 233 1094 3473 3657 233
Size: 861 2379 184 1378
Fragments arranged by size:
2379 1378 861 184
MscI TGG'CCA
Cuts at: 796 1351 1573 2563 796
Size: 555 222 990 3035
Fragments arranged by size:
3035 990 555 222
MslI CAynn'nnrTG
Cuts at: 847 1312 2791 2918 847
Size: 465 1479 127 2731
Fragments arranged by size:
2731 1479 465 127
MspA1I CmG'CkG
Cuts at: 781 945 1227 1913 2227 2587 3600 3845 781
Size: 164 282 686 314 360 1013 245 1738
Fragments arranged by size:
1738 1013 686 360 314 282 245 164
NaeI GCC'GGC
Cuts at: 2983 2983
Size: 4802
NarI GG'CG_CC
Cuts at: 2233 2481 2233
Size: 248 4554
NcoI C'CATG_G
Cuts at: 107 791 1331 1547 1574 107
Size: 684 540 216 27 3335
Fragments arranged by size:
3335 684 540 216 27
NgoMIV G'CCGG_C
Cuts at: 2981 2981
Size: 4802
NspI r_CATG'y
Cuts at: 1855 2355 3262 1855
Size: 500 907 3395
Fragments arranged by size:
3395 907 500
PciI A'CATG_T
Cuts at: 3258 3258
Size: 4802
PfoI T'CCnGG_A
Cuts at: 417 417
Size: 4802
PmeI GTTT'AAAC
Cuts at: 1801 1801
Size: 4802
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1067 1099 3941 3973 1067
Size: 32 2842 32 1896
Fragments arranged by size:
2842 1896 32 32
PstI C_TGCA'G
Cuts at: 613 1849 2534 613
Size: 1236 685 2881
Fragments arranged by size:
2881 1236 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4802
PvuII CAG'CTG
Cuts at: 2227 2587 2227
Size: 360 4442
RsrII CG'GwC_CG
Cuts at: 2997 2997
Size: 4802
SacI G_AGCT'C
Cuts at: 654 1504 1816 654
Size: 850 312 3640
Fragments arranged by size:
3640 850 312
SalI G'TCGA_C
Cuts at: 1839 1839
Size: 4802
SapI GCTCTTCn'nnn_
Cuts at: 1417 2825 3035 1417
Size: 1408 210 3184
Fragments arranged by size:
3184 1408 210
SbfI CC_TGCA'GG
Cuts at: 1849 1849
Size: 4802
ScaI AGT'ACT
Cuts at: 4319 4319
Size: 4802
SfcI C'TryA_G
Cuts at: 33 329 609 1845 2530 3523 3714 33
Size: 296 280 1236 685 993 191 1121
Fragments arranged by size:
1236 1121 993 685 296 280 191
SfoI GGC'GCC
Cuts at: 2234 2482 2234
Size: 248 4554
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4802
SmaI CCC'GGG
Cuts at: 1824 1824
Size: 4802
SmlI C'TyrA_G
Cuts at: 1248 1957 2872 3364 3626 1248
Size: 709 915 492 262 2424
Fragments arranged by size:
2424 915 709 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4802
SphI G_CATG'C
Cuts at: 1855 2355 1855
Size: 500 4302
SspI AAT'ATT
Cuts at: 1341 1341
Size: 4802
StyI C'CwwG_G
Cuts at: 107 791 1331 1547 1574 1936 2290 107
Size: 684 540 216 27 362 354 2619
Fragments arranged by size:
2619 684 540 362 354 216 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 520 705 1311 2385 2518 2977 3186 520
Size: 185 606 1074 133 459 209 2136
Fragments arranged by size:
2136 1074 606 459 209 185 133
TatI w'GTAC_w
Cuts at: 1525 4317 1525
Size: 2792 2010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 413 445 4319 4351 413
Size: 32 3874 32 864
Fragments arranged by size:
3874 864 32 32
Tth111I GACn'n_nGTC
Cuts at: 1024 2599 1024
Size: 1575 3227
XbaI T'CTAG_A
Cuts at: 1833 1833
Size: 4802
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1311 2297 1311
Size: 986 3816
XmaI C'CCGG_G
Cuts at: 1822 1822
Size: 4802
XmnI GAAnn'nnTTC
Cuts at: 1079 1079
Size: 4802
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI