Restriction map for
pF1KB8176
(Circular) (Six-base) MAPSORT of: pF1KB8176.seq Check: 8656 from: 1 to: 3848
pF1KB8176 3848 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 187 187
Size: 3848
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 488 864 488
Size: 376 3472
AclI AA'CG_TT
Cuts at: 842 3688 842
Size: 2846 1002
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1673 2105 2852 3120 518
Size: 1155 432 747 268 1246
Fragments arranged by size:
1246 1155 747 432 268
AfeI AGC'GCT
Cuts at: 160 160
Size: 3848
AflIII A'CryG_T
Cuts at: 1200 2304 3281 1200
Size: 1104 977 1767
Fragments arranged by size:
1767 1104 977
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AleI CACnn'nnGTG
Cuts at: 394 394
Size: 3848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 1153 2720 1153
Size: 1567 2281
ApaLI G'TGCA_C
Cuts at: 2618 2618
Size: 3848
ApoI r'AATT_y
Cuts at: 852 3201 3457 852
Size: 2349 256 1243
Fragments arranged by size:
2349 1243 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 868 868
Size: 3848
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 205 488 864 1278 1526 1561 205
Size: 283 376 414 248 35 2492
Fragments arranged by size:
2492 414 376 283 248 35
BanII G_rGCy'C
Cuts at: 862 1892 862
Size: 1030 2818
BbeI G_GCGC'C
Cuts at: 209 1282 1530 209
Size: 1073 248 2527
Fragments arranged by size:
2527 1073 248
BbsI GAAGACnn'nnnn_
Cuts at: 121 316 447 737 121
Size: 195 131 290 3232
Fragments arranged by size:
3232 290 195 131
BciVI GTATCCnnnnn_n'
Cuts at: 1746 2507 3848 1746
Size: 761 1341 1746
Fragments arranged by size:
1746 1341 761
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglI GCCn_nnn'nGGC
Cuts at: 142 151 142
Size: 9 3839
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 960 960
Size: 3848
Bme1580I G_kGCm'C
Cuts at: 1473 1566 2622 1473
Size: 93 1056 2699
Fragments arranged by size:
2699 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 303
Size: 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1903 2395 2693 1024
Size: 879 492 298 2179
Fragments arranged by size:
2179 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 206 1279 1527 2229 3725 206
Size: 1073 248 702 1496 329
Fragments arranged by size:
1496 1073 702 329 248
BsaWI w'CCGG_w
Cuts at: 485 1037 1558 2236 2510 2657 3068 485
Size: 552 521 678 274 147 411 1265
Fragments arranged by size:
1265 678 552 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 223
Size: 30 3818
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 238
Size: 3848
BseYI C'CCAG_C
Cuts at: 1273 2031 2608 3104 1273
Size: 758 577 496 2017
Fragments arranged by size:
2017 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 372 657 824 372
Size: 285 167 3396
Fragments arranged by size:
3396 285 167
BsiEI CG_ry'CG
Cuts at: 104 1390 1436 2644 104
Size: 1286 46 1208 1308
Fragments arranged by size:
1308 1286 1208 46
BsiHKAI G_wGCw'C
Cuts at: 862 1640 1830 2622 862
Size: 778 190 792 2088
Fragments arranged by size:
2088 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 862 1473 1566 1640 1830 1892 2622 862
Size: 611 93 74 190 62 730 2088
Fragments arranged by size:
2088 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 187 242 263 898 1112 1414 1795 187
Size: 55 21 635 214 302 381 2240
Fragments arranged by size:
2240 635 381 302 214 55 21
BsrBI CCG'CTC
Cuts at: 50 2140 2194 3678 3843 50
Size: 2090 54 1484 165 55
Fragments arranged by size:
2090 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 704 1760 704
Size: 1056 2792
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BssSI C'ACGA_G
Cuts at: 2119 2477 2119
Size: 358 3490
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstXI CCAn_nnnn'nTGG
Cuts at: 399 399
Size: 3848
BstYI r'GATC_y
Cuts at: 1 379 873 1340 1363 1698 1944 2956 3042
Size: 378 494 467 23 335 246 1012 86
Cuts at: 3042 3054 3400 1
Size: 12 346 449
Fragments arranged by size:
1012 494 467 449 378 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 242 392 107
Size: 135 150 3563
Fragments arranged by size:
3563 150 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 197 1091 197
Size: 894 2954
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 143 173 1433 1607 1998 2025 3370 143
Size: 30 1260 174 391 27 1345 621
Fragments arranged by size:
1345 1260 621 391 174 30 27
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 267 595 2056 2366 2512 3658 267
Size: 328 1461 310 146 1146 457
Fragments arranged by size:
1461 1146 457 328 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 518 1673 2105 2852 3120 303
Size: 215 1155 432 747 268 1031
Fragments arranged by size:
1155 1031 747 432 268 215
EcoO109I rG'GnC_Cy
Cuts at: 987 987
Size: 3848
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3848
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 162 209 1282 1530 2552 3531 162
Size: 47 1073 248 1022 979 479
Fragments arranged by size:
1073 1022 979 479 248 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 1690 1720 1722 1752 364
Size: 32 1294 30 2 30 2460
Fragments arranged by size:
2460 1294 32 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
KasI G'GCGC_C
Cuts at: 205 1278 1526 205
Size: 1073 248 2527
Fragments arranged by size:
2527 1073 248
KpnI G_GTAC'C
Cuts at: 492 868 492
Size: 376 3472
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 242 347 389 2519 2703 242
Size: 105 42 2130 184 1387
Fragments arranged by size:
2130 1387 184 105 42
MscI TGG'CCA
Cuts at: 175 1609 175
Size: 1434 2414
MslI CAynn'nnrTG
Cuts at: 181 394 1837 1964 181
Size: 213 1443 127 2065
Fragments arranged by size:
2065 1443 213 127
MspA1I CmG'CkG
Cuts at: 339 959 1273 1633 2646 2891 339
Size: 620 314 360 1013 245 1296
Fragments arranged by size:
1296 1013 620 360 314 245
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 206 1279 1527 206
Size: 1073 248 2527
Fragments arranged by size:
2527 1073 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NspI r_CATG'y
Cuts at: 901 1401 2308 901
Size: 500 907 2441
Fragments arranged by size:
2441 907 500
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PstI C_TGCA'G
Cuts at: 895 1580 895
Size: 685 3163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 339 1273 1633 339
Size: 934 360 2554
Fragments arranged by size:
2554 934 360
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SfcI C'TryA_G
Cuts at: 33 891 1576 2569 2760 33
Size: 858 685 993 191 1121
Fragments arranged by size:
1121 993 858 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 142 151 142
Size: 9 3839
SfoI GGC'GCC
Cuts at: 207 1280 1528 207
Size: 1073 248 2527
Fragments arranged by size:
2527 1073 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 870 870
Size: 3848
SmlI C'TyrA_G
Cuts at: 1003 1918 2410 2672 1003
Size: 915 492 262 2179
Fragments arranged by size:
2179 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
SspI AAT'ATT
Cuts at: 418 418
Size: 3848
StyI C'CwwG_G
Cuts at: 107 543 982 1336 107
Size: 436 439 354 2619
Fragments arranged by size:
2619 439 436 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 211 328 614 1431 1564 2023 2232 211
Size: 117 286 817 133 459 209 1827
Fragments arranged by size:
1827 817 459 286 209 133 117
TatI w'GTAC_w
Cuts at: 3363 3363
Size: 3848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 587 619 3365 3397 587
Size: 32 2746 32 1038
Fragments arranged by size:
2746 1038 32 32
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1343 1343
Size: 3848
XmaI C'CCGG_G
Cuts at: 868 868
Size: 3848
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI