Restriction map for
pF1KB8179
(Circular) (Six-base) MAPSORT of: pF1KB8179.seq Check: 7294 from: 1 to: 4415
pF1KB8179 4415 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1001 1001
Size: 4415
AatII G_ACGT'C
Cuts at: 1267 1267
Size: 4415
AccI GT'mk_AC
Cuts at: 1453 1453
Size: 4415
Acc65I G'GTAC_C
Cuts at: 1223 1431 1223
Size: 208 4207
AclI AA'CG_TT
Cuts at: 4255 4255
Size: 4415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 1372 2240 2672 3419 3687 455
Size: 917 868 432 747 268 1183
Fragments arranged by size:
1183 917 868 747 432 268
AflIII A'CryG_T
Cuts at: 1767 2871 3848 1767
Size: 1104 977 2334
Fragments arranged by size:
2334 1104 977
AgeI A'CCGG_T
Cuts at: 2803 2803
Size: 4415
AleI CACnn'nnGTG
Cuts at: 359 359
Size: 4415
AlwNI CAG_nnn'CTG
Cuts at: 330 495 588 783 1010 1720 3287 330
Size: 165 93 195 227 710 1567 1458
Fragments arranged by size:
1567 1458 710 227 195 165 93
ApaLI G'TGCA_C
Cuts at: 1158 3185 1158
Size: 2027 2388
ApoI r'AATT_y
Cuts at: 1419 3768 4024 1419
Size: 2349 256 1810
Fragments arranged by size:
2349 1810 256
AseI AT'TA_AT
Cuts at: 20 1337 20
Size: 1317 3098
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 368 1037 1313 1435 368
Size: 669 276 122 3348
Fragments arranged by size:
3348 669 276 122
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 598 631 598
Size: 33 4382
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 1223 1431 1845 2093 2128 1223
Size: 208 414 248 35 3510
Fragments arranged by size:
3510 414 248 208 35
BanII G_rGCy'C
Cuts at: 1429 2459 1429
Size: 1030 3385
BbeI G_GCGC'C
Cuts at: 1849 2097 1849
Size: 248 4167
BbvCI CC'TCA_GC
Cuts at: 877 877
Size: 4415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1389 1423 1389
Size: 34 4381
BciVI GTATCCnnnnn_n'
Cuts at: 375 2313 3074 4415 375
Size: 1938 761 1341 375
Fragments arranged by size:
1938 1341 761 375
BclI T'GATC_A
Cuts at: 1935 1935
Size: 4415
BglII A'GATC_T
Cuts at: 530 1019 1930 3967 530
Size: 489 911 2037 978
Fragments arranged by size:
2037 978 911 489
BlpI GC'TnA_GC
Cuts at: 230 631 1527 230
Size: 401 896 3118
Fragments arranged by size:
3118 896 401
Bme1580I G_kGCm'C
Cuts at: 481 1162 2040 2133 3189 481
Size: 681 878 93 1056 1707
Fragments arranged by size:
1707 1056 878 681 93
BmrI ACTGGGnnnn_n'
Cuts at: 1020 1172 1627 1708 2043 4187 1020
Size: 152 455 81 335 2144 1248
Fragments arranged by size:
2144 1248 455 335 152 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1339 2782 1339
Size: 1443 2972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 235 395 826 889 214
Size: 21 160 431 63 3740
Fragments arranged by size:
3740 431 160 63 21
Bpu10I CC'TnA_GC
Cuts at: 877 1081 877
Size: 204 4211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 2470 2962 3260 1591
Size: 879 492 298 2746
Fragments arranged by size:
2746 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1021 4119 1021
Size: 3098 1317
BsaAI yAC'GTr
Cuts at: 213 401 1768 213
Size: 188 1367 2860
Fragments arranged by size:
2860 1367 188
BsaBI GATnn'nnATC
Cuts at: 6 598 1129 1929 3966 6
Size: 592 531 800 2037 455
Fragments arranged by size:
2037 800 592 531 455
BsaHI Gr'CG_yC
Cuts at: 1043 1264 1846 2094 2796 4292 1043
Size: 221 582 248 702 1496 1166
Fragments arranged by size:
1496 1166 702 582 248 221
BsaWI w'CCGG_w
Cuts at: 1604 2125 2803 3077 3224 3635 1604
Size: 521 678 274 147 411 2384
Fragments arranged by size:
2384 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 493 658 211
Size: 282 165 3968
Fragments arranged by size:
3968 282 165
BseYI C'CCAG_C
Cuts at: 439 963 1056 1840 2598 3175 3671 439
Size: 524 93 784 758 577 496 1183
Fragments arranged by size:
1183 784 758 577 524 496 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 343 712 802 811 898 190
Size: 153 369 90 9 87 3707
Fragments arranged by size:
3707 369 153 90 87 9
BsiEI CG_ry'CG
Cuts at: 104 1957 2003 3211 104
Size: 1853 46 1208 1308
Fragments arranged by size:
1853 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 400 431 1162 1429 2207 2397 3189 400
Size: 31 731 267 778 190 792 1626
Fragments arranged by size:
1626 792 778 731 267 190 31
BsmBI CGTCTCn'nnnn_
Cuts at: 771 771
Size: 4415
Bsp1286I G_dGCh'C
Cuts at: 400 431 481 1162 1429 2040 2133 2207 2397
Size: 31 50 681 267 611 93 74 190
Cuts at: 2397 2459 3189 400
Size: 62 730 1626
Fragments arranged by size:
1626 730 681 611 267 190 93 74
62 50 31
BspEI T'CCGG_A
Cuts at: 3635 3635
Size: 4415
BspHI T'CATG_A
Cuts at: 3591 3591
Size: 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 965 1001 1465 1679 1981 2362 965
Size: 36 464 214 302 381 3018
Fragments arranged by size:
3018 464 381 302 214 36
BsrBI CCG'CTC
Cuts at: 50 186 2707 2761 4245 4410 50
Size: 136 2521 54 1484 165 55
Fragments arranged by size:
2521 1484 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 2327 2327
Size: 4415
BsrFI r'CCGG_y
Cuts at: 405 2413 2594 2803 405
Size: 2008 181 209 2017
Fragments arranged by size:
2017 2008 209 181
BsrGI T'GTAC_A
Cuts at: 446 446
Size: 4415
BssSI C'ACGA_G
Cuts at: 2686 3044 2686
Size: 358 4057
BstAPI GCAn_nnn'nTGC
Cuts at: 588 627 783 825 1217 1792 588
Size: 39 156 42 392 575 3211
Fragments arranged by size:
3211 575 392 156 42 39
BstBI TT'CG_AA
Cuts at: 2776 2776
Size: 4415
BstYI r'GATC_y
Cuts at: 1 530 584 926 1019 1440 1907 1930 2265
Size: 529 54 342 93 421 467 23 335
Cuts at: 2265 2511 3523 3609 3621 3967 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 529 467 449 421 346 342 335
246 93 86 54 23 12
BtgI C'CryG_G
Cuts at: 107 1370 107
Size: 1263 3152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2365 2546 2709 2365
Size: 181 163 4071
Fragments arranged by size:
4071 181 163
BtsI GCAGTG_nn'
Cuts at: 121 366 1162 1658 121
Size: 245 796 496 2878
Fragments arranged by size:
2878 796 496 245
DraI TTT'AAA
Cuts at: 1414 1414
Size: 4415
DraIII CAC_nnn'GTG
Cuts at: 213 1233 213
Size: 1020 3395
DrdI GACnn_nn'nnGTC
Cuts at: 2121 2979 2121
Size: 858 3557
EaeI y'GGCC_r
Cuts at: 236 403 596 638 1299 2000 2174 2565 2592
Size: 167 193 42 661 701 174 391 27
Cuts at: 2592 3937 236
Size: 1345 714
Fragments arranged by size:
1345 714 701 661 391 193 174 167
42 27
EagI C'GGCC_G
Cuts at: 2000 2000
Size: 4415
EarI CTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 826 1243 2623 2933 3079 4225 826
Size: 417 1380 310 146 1146 1016
Fragments arranged by size:
1380 1146 1016 417 310 146
EcoICRI GAG'CTC
Cuts at: 1427 1427
Size: 4415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 235 395 455 826 889 1372 2240 2672
Size: 21 160 60 371 63 483 868 432
Cuts at: 2672 3419 3687 214
Size: 747 268 942
Fragments arranged by size:
942 868 747 483 432 371 268 160
63 60 21
EcoO109I rG'GnC_Cy
Cuts at: 147 257 492 745 853 873 1554 147
Size: 110 235 253 108 20 681 3008
Fragments arranged by size:
3008 681 253 235 110 108 20
EcoRI G'AATT_C
Cuts at: 1419 1419
Size: 4415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4415
FspI TGC'GCA
Cuts at: 2196 2196
Size: 4415
HaeII r_GCGC'y
Cuts at: 783 1114 1849 2097 3119 4098 783
Size: 331 735 248 1022 979 1100
Fragments arranged by size:
1100 1022 979 735 331 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2257 2287 2289 2319 2257
Size: 30 2 30 4353
Fragments arranged by size:
4353 30 30 2
HincII GTy'rAC
Cuts at: 1454 1454
Size: 4415
KasI G'GCGC_C
Cuts at: 1845 2093 1845
Size: 248 4167
KpnI G_GTAC'C
Cuts at: 1227 1435 1227
Size: 208 4207
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 734 1298 3086 3270 734
Size: 564 1788 184 1879
Fragments arranged by size:
1879 1788 564 184
MscI TGG'CCA
Cuts at: 238 598 1301 2176 238
Size: 360 703 875 2477
Fragments arranged by size:
2477 875 703 360
MslI CAynn'nnrTG
Cuts at: 359 2404 2531 359
Size: 2045 127 2243
Fragments arranged by size:
2243 2045 127
MspA1I CmG'CkG
Cuts at: 222 867 921 1152 1526 1840 2200 3213 3458
Size: 645 54 231 374 314 360 1013 245
Cuts at: 3458 222
Size: 1179
Fragments arranged by size:
1179 1013 645 374 360 314 245 231
54
NaeI GCC'GGC
Cuts at: 407 2596 407
Size: 2189 2226
NarI GG'CG_CC
Cuts at: 1846 2094 1846
Size: 248 4167
NcoI C'CATG_G
Cuts at: 107 1370 107
Size: 1263 3152
NgoMIV G'CCGG_C
Cuts at: 405 2594 405
Size: 2189 2226
NspI r_CATG'y
Cuts at: 630 1468 1968 2875 630
Size: 838 500 907 2170
Fragments arranged by size:
2170 907 838 500
PciI A'CATG_T
Cuts at: 2871 2871
Size: 4415
PflMI CCAn_nnn'nTGG
Cuts at: 996 996
Size: 4415
PfoI T'CCnGG_A
Cuts at: 716 716
Size: 4415
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PmlI CAC'GTG
Cuts at: 401 401
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 508 540 3554 3586 508
Size: 32 3014 32 1337
Fragments arranged by size:
3014 1337 32 32
PpuMI rG'GwC_Cy
Cuts at: 147 257 745 147
Size: 110 488 3817
Fragments arranged by size:
3817 488 110
PshAI GACnn'nnGTC
Cuts at: 743 743
Size: 4415
PstI C_TGCA'G
Cuts at: 155 593 1462 2147 155
Size: 438 869 685 2423
Fragments arranged by size:
2423 869 685 438
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 222 867 1840 2200 222
Size: 645 973 360 2437
Fragments arranged by size:
2437 973 645 360
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 1429 1429
Size: 4415
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SanDI GG'GwC_CC
Cuts at: 257 257
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SfcI C'TryA_G
Cuts at: 33 151 589 1458 2143 3136 3327 33
Size: 118 438 869 685 993 191 1121
Fragments arranged by size:
1121 993 869 685 438 191 118
SfoI GGC'GCC
Cuts at: 1847 2095 1847
Size: 248 4167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 1039 1437 1039
Size: 398 4017
SmlI C'TyrA_G
Cuts at: 1570 2485 2977 3239 1570
Size: 915 492 262 2746
Fragments arranged by size:
2746 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 630 1468 1968 630
Size: 838 500 3077
Fragments arranged by size:
3077 838 500
StyI C'CwwG_G
Cuts at: 107 1370 1549 1903 107
Size: 1263 179 354 2619
Fragments arranged by size:
2619 1263 354 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1998 2131 2590 2799 1998
Size: 133 459 209 3614
Fragments arranged by size:
3614 459 209 133
TatI w'GTAC_w
Cuts at: 446 3930 446
Size: 3484 931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 4383
Tth111I GACn'n_nGTC
Cuts at: 145 2212 145
Size: 2067 2348
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 279 419 1910 279
Size: 140 1491 2784
Fragments arranged by size:
2784 1491 140
XmaI C'CCGG_G
Cuts at: 1037 1435 1037
Size: 398 4017
ZraI GAC'GTC
Cuts at: 1265 1265
Size: 4415
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI ApaI AscI AvrII
BbsI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI