Restriction map for
pF1KB8183
(Circular) (Six-base) MAPSORT of: pF1KB8183.seq Check: 8161 from: 1 to: 3725
pF1KB8183 3725 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1550 1982 2729 2997 530
Size: 1020 432 747 268 1258
Fragments arranged by size:
1258 1020 747 432 268
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 2113 2113
Size: 3725
AlwNI CAG_nnn'CTG
Cuts at: 375 1030 2597 375
Size: 655 1567 1503
Fragments arranged by size:
1567 1503 655
ApaI G_GGCC'C
Cuts at: 157 157
Size: 3725
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 729 3078 3334 729
Size: 2349 256 1120
Fragments arranged by size:
2349 1120 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 156 205 745 156
Size: 49 540 3136
Fragments arranged by size:
3136 540 49
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 136 710 741 1155 1403 1438 136
Size: 574 31 414 248 35 2423
Fragments arranged by size:
2423 574 414 248 35 31
BanII G_rGCy'C
Cuts at: 157 205 739 1769 157
Size: 48 534 1030 2113
Fragments arranged by size:
2113 1030 534 48
BbeI G_GCGC'C
Cuts at: 140 714 1159 1407 140
Size: 574 445 248 2458
Fragments arranged by size:
2458 574 445 248
BbsI GAAGACnn'nnnn_
Cuts at: 509 509
Size: 3725
BciVI GTATCCnnnnn_n'
Cuts at: 1623 2384 3725 1623
Size: 761 1341 1623
Fragments arranged by size:
1623 1341 761
BclI T'GATC_A
Cuts at: 1245 1245
Size: 3725
BglII A'GATC_T
Cuts at: 1240 3277 1240
Size: 2037 1688
BlpI GC'TnA_GC
Cuts at: 837 837
Size: 3725
Bme1580I G_kGCm'C
Cuts at: 157 1350 1443 2499 157
Size: 1193 93 1056 1383
Fragments arranged by size:
1383 1193 1056 93
BmgBI CAC'GTC
Cuts at: 507 507
Size: 3725
BmrI ACTGGGnnnn_n'
Cuts at: 689 937 1018 1353 3497 689
Size: 248 81 335 2144 917
Fragments arranged by size:
2144 917 335 248 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 615 2092 615
Size: 1477 2248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1780 2272 2570 901
Size: 879 492 298 2056
Fragments arranged by size:
2056 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3429 3429
Size: 3725
BsaAI yAC'GTr
Cuts at: 1078 1078
Size: 3725
BsaBI GATnn'nnATC
Cuts at: 6 1239 3276 6
Size: 1233 2037 455
Fragments arranged by size:
2037 1233 455
BsaHI Gr'CG_yC
Cuts at: 137 711 1156 1404 2106 3602 137
Size: 574 445 248 702 1496 260
Fragments arranged by size:
1496 702 574 445 260 248
BsaWI w'CCGG_w
Cuts at: 914 1435 2113 2387 2534 2945 914
Size: 521 678 274 147 411 1694
Fragments arranged by size:
1694 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 493 505 591 199
Size: 294 12 86 3333
Fragments arranged by size:
3333 294 86 12
BseYI C'CCAG_C
Cuts at: 237 1150 1908 2485 2981 237
Size: 913 758 577 496 981
Fragments arranged by size:
981 913 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1267 1313 2521 104
Size: 1163 46 1208 1308
Fragments arranged by size:
1308 1208 1163 46
BsiHKAI G_wGCw'C
Cuts at: 355 739 1517 1707 2499 355
Size: 384 778 190 792 1581
Fragments arranged by size:
1581 792 778 384 190
Bsp1286I G_dGCh'C
Cuts at: 157 205 355 739 1350 1443 1517 1707 1769
Size: 48 150 384 611 93 74 190 62
Cuts at: 1769 2499 157
Size: 730 1383
Fragments arranged by size:
1383 730 611 384 190 150 93 74
62 48
BspEI T'CCGG_A
Cuts at: 2945 2945
Size: 3725
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 309 775 989 1291 1672 309
Size: 466 214 302 381 2362
Fragments arranged by size:
2362 466 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2017 2071 3555 3720 50
Size: 1967 54 1484 165 55
Fragments arranged by size:
1967 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 1637
Size: 3725
BsrFI r'CCGG_y
Cuts at: 1723 1904 2113 1723
Size: 181 209 3335
Fragments arranged by size:
3335 209 181
BssSI C'ACGA_G
Cuts at: 1996 2354 1996
Size: 358 3367
BstAPI GCAn_nnn'nTGC
Cuts at: 375 1102 375
Size: 727 2998
BstBI TT'CG_AA
Cuts at: 2086 2086
Size: 3725
BstEII G'GTnAC_C
Cuts at: 651 651
Size: 3725
BstYI r'GATC_y
Cuts at: 1 750 1217 1240 1575 1821 2833 2919 2931
Size: 749 467 23 335 246 1012 86 12
Cuts at: 2931 3277 1
Size: 346 449
Fragments arranged by size:
1012 749 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3725
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1675 1856 2019 1675
Size: 181 163 3381
Fragments arranged by size:
3381 181 163
BtsI GCAGTG_nn'
Cuts at: 968 968
Size: 3725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 262 297 262
Size: 35 3690
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 1431 2289 1431
Size: 858 2867
EaeI y'GGCC_r
Cuts at: 167 1310 1484 1875 1902 3247 167
Size: 1143 174 391 27 1345 645
Fragments arranged by size:
1345 1143 645 391 174 27
EagI C'GGCC_G
Cuts at: 1310 1310
Size: 3725
EarI CTCTTCn'nnn_
Cuts at: 229 428 470 1748 1958 229
Size: 199 42 1278 210 1996
Fragments arranged by size:
1996 1278 210 199 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 1933 2243 2389 3535 196
Size: 1737 310 146 1146 386
Fragments arranged by size:
1737 1146 386 310 146
EcoICRI GAG'CTC
Cuts at: 737 737
Size: 3725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1550 1982 2729 2997 530
Size: 1020 432 747 268 1258
Fragments arranged by size:
1258 1020 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 153 321 864 153
Size: 168 543 3014
Fragments arranged by size:
3014 543 168
EcoRI G'AATT_C
Cuts at: 729 729
Size: 3725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1744 1744
Size: 3725
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 140 376 714 1159 1407 2429 3408 140
Size: 236 338 445 248 1022 979 457
Fragments arranged by size:
1022 979 457 445 338 248 236
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1567 1597 1599 1629 1567
Size: 30 2 30 3663
Fragments arranged by size:
3663 30 30 2
HincII GTy'rAC
Cuts at: 764 764
Size: 3725
KasI G'GCGC_C
Cuts at: 136 710 1155 1403 136
Size: 574 445 248 2458
Fragments arranged by size:
2458 574 445 248
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2580 2396
Size: 184 3541
MscI TGG'CCA
Cuts at: 1486 1486
Size: 3725
MslI CAynn'nnrTG
Cuts at: 1714 1841 1714
Size: 127 3598
MspA1I CmG'CkG
Cuts at: 836 1150 1510 2523 2768 836
Size: 314 360 1013 245 1793
Fragments arranged by size:
1793 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1906 1906
Size: 3725
NarI GG'CG_CC
Cuts at: 137 711 1156 1404 137
Size: 574 445 248 2458
Fragments arranged by size:
2458 574 445 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3725
NgoMIV G'CCGG_C
Cuts at: 1904 1904
Size: 3725
NspI r_CATG'y
Cuts at: 369 778 1278 2185 369
Size: 409 500 907 1909
Fragments arranged by size:
1909 907 500 409
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 3693
PpuMI rG'GwC_Cy
Cuts at: 321 321
Size: 3725
PspOMI G'GGCC_C
Cuts at: 153 153
Size: 3725
PstI C_TGCA'G
Cuts at: 772 1457 772
Size: 685 3040
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 1150 1510 1150
Size: 360 3365
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 739 739
Size: 3725
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 768 1453 2446 2637 33
Size: 735 685 993 191 1121
Fragments arranged by size:
1121 993 735 685 191
SfoI GGC'GCC
Cuts at: 138 712 1157 1405 138
Size: 574 445 248 2458
Fragments arranged by size:
2458 574 445 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 158 747 158
Size: 589 3136
SmlI C'TyrA_G
Cuts at: 880 1795 2287 2549 880
Size: 915 492 262 2056
Fragments arranged by size:
2056 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 778 1278 778
Size: 500 3225
StyI C'CwwG_G
Cuts at: 107 329 859 1213 107
Size: 222 530 354 2619
Fragments arranged by size:
2619 530 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1308 1441 1900 2109 1308
Size: 133 459 209 2924
Fragments arranged by size:
2924 459 209 133
TatI w'GTAC_w
Cuts at: 3240 3240
Size: 3725
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 3693
Tth111I GACn'n_nGTC
Cuts at: 661 1522 661
Size: 861 2864
XbaI T'CTAG_A
Cuts at: 756 756
Size: 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1220 1220
Size: 3725
XmaI C'CCGG_G
Cuts at: 156 745 156
Size: 589 3136
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI