Restriction map for
pF1KB8185
(Circular) (Six-base) MAPSORT of: pF1KB8185.seq Check: 1084 from: 1 to: 4496
pF1KB8185 4496 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1258 2321 2753 3500 3768 763
Size: 495 1063 432 747 268 1491
Fragments arranged by size:
1491 1063 747 495 432 268
AflIII A'CryG_T
Cuts at: 1848 2952 3929 1848
Size: 1104 977 2415
Fragments arranged by size:
2415 1104 977
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 897 1801 3368 897
Size: 904 1567 2025
Fragments arranged by size:
2025 1567 904
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 1500 3849 4105 1500
Size: 2349 256 1891
Fragments arranged by size:
2349 1891 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1516 1516
Size: 4496
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 668 841 1512 1926 2174 2209 668
Size: 173 671 414 248 35 2955
Fragments arranged by size:
2955 671 414 248 173 35
BanII G_rGCy'C
Cuts at: 1510 2540 1510
Size: 1030 3466
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 769 1046 769
Size: 277 4219
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglI GCCn_nnn'nGGC
Cuts at: 484 484
Size: 4496
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 434 535 844 2121 2214 3270 434
Size: 101 309 1277 93 1056 1660
Fragments arranged by size:
1660 1277 1056 309 101 93
BmrI ACTGGGnnnn_n'
Cuts at: 1708 1789 2124 4268 1708
Size: 81 335 2144 1936
Fragments arranged by size:
2144 1936 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 410 800 1240 1491 362
Size: 48 390 440 251 3367
Fragments arranged by size:
3367 440 390 251 48
Bpu10I CC'TnA_GC
Cuts at: 202 202
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 736 754 1402 1672 2551 3043 3341 661
Size: 75 18 648 270 879 492 298 1816
Fragments arranged by size:
1816 879 648 492 298 270 75 18
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 1849 1849
Size: 4496
BsaBI GATnn'nnATC
Cuts at: 6 1270 2010 4047 6
Size: 1264 740 2037 455
Fragments arranged by size:
2037 1264 740 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3158 3305 3716 1685
Size: 521 678 274 147 411 2465
Fragments arranged by size:
2465 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1056 1086 1056
Size: 30 4466
BseYI C'CCAG_C
Cuts at: 433 497 660 1921 2679 3256 3752 433
Size: 64 163 1261 758 577 496 1177
Fragments arranged by size:
1261 1177 758 577 496 163 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 1435
Size: 4496
BsiEI CG_ry'CG
Cuts at: 104 762 2038 2084 3292 104
Size: 658 1276 46 1208 1308
Fragments arranged by size:
1308 1276 1208 658 46
BsiHKAI G_wGCw'C
Cuts at: 1510 2288 2478 3270 1510
Size: 778 190 792 2736
Fragments arranged by size:
2736 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 434 535 844 1510 2121 2214 2288 2478 2540
Size: 101 309 666 611 93 74 190 62
Cuts at: 2540 3270 434
Size: 730 1660
Fragments arranged by size:
1660 730 666 611 309 190 101 93
74 62
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 971 3672 971
Size: 2701 1795
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 1760 2062 2443 1546
Size: 214 302 381 3599
Fragments arranged by size:
3599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 291 1250 1407 2788 2842 4326 4491 50
Size: 241 959 157 1381 54 1484 165 55
Fragments arranged by size:
1484 1381 959 241 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 718 828 2408 718
Size: 110 1580 2806
Fragments arranged by size:
2806 1580 110
BsrFI r'CCGG_y
Cuts at: 665 2494 2675 2884 665
Size: 1829 181 209 2277
Fragments arranged by size:
2277 1829 209 181
BssSI C'ACGA_G
Cuts at: 2767 3125 2767
Size: 358 4138
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstYI r'GATC_y
Cuts at: 1 197 308 1223 1521 1988 2011 2346 2592
Size: 196 111 915 298 467 23 335 246
Cuts at: 2592 3604 3690 3702 4048 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 915 467 449 346 335 298 246
196 111 86 23 12
BtgI C'CryG_G
Cuts at: 107 682 107
Size: 575 3921
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 494 1739 494
Size: 1245 3251
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DraIII CAC_nnn'GTG
Cuts at: 1005 1005
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 625 2202 3060 625
Size: 1577 858 2061
Fragments arranged by size:
2061 1577 858
EaeI y'GGCC_r
Cuts at: 191 222 759 908 1398 2081 2255 2646 2673
Size: 31 537 149 490 683 174 391 27
Cuts at: 2673 4018 191
Size: 1345 669
Fragments arranged by size:
1345 683 669 537 490 391 174 149
31 27
EagI C'GGCC_G
Cuts at: 759 2081 759
Size: 1322 3174
EarI CTCTTCn'nnn_
Cuts at: 967 2519 2729 967
Size: 1552 210 2734
Fragments arranged by size:
2734 1552 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 174 2704 3014 3160 4306 127
Size: 47 2530 310 146 1146 317
Fragments arranged by size:
2530 1146 317 310 146 47
EcoICRI GAG'CTC
Cuts at: 1508 1508
Size: 4496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 410 763 800 1240 1258 1491 2321 2753
Size: 48 353 37 440 18 233 830 432
Cuts at: 2753 3500 3768 362
Size: 747 268 1090
Fragments arranged by size:
1090 830 747 440 432 353 268 233
48 37 18
EcoO109I rG'GnC_Cy
Cuts at: 362 1151 1635 362
Size: 789 484 3223
Fragments arranged by size:
3223 789 484
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4496
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1086 1930 2178 3200 4179 1086
Size: 844 248 1022 979 1403
Fragments arranged by size:
1403 1022 979 844 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 529 561 1015 1047 1303 1335 2338 2368 2370
Size: 32 454 32 256 32 1003 30 2
Cuts at: 2370 2400 529
Size: 30 2625
Fragments arranged by size:
2625 1003 454 256 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
HindIII A'AGCT_T
Cuts at: 229 229
Size: 4496
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1223 3167 3351 1223
Size: 1944 184 2368
Fragments arranged by size:
2368 1944 184
MscI TGG'CCA
Cuts at: 910 1400 2257 910
Size: 490 857 3149
Fragments arranged by size:
3149 857 490
MslI CAynn'nnrTG
Cuts at: 634 2485 2612 634
Size: 1851 127 2518
Fragments arranged by size:
2518 1851 127
MspA1I CmG'CkG
Cuts at: 1607 1921 2281 3294 3539 1607
Size: 314 360 1013 245 2564
Fragments arranged by size:
2564 1013 360 314 245
NaeI GCC'GGC
Cuts at: 667 2677 667
Size: 2010 2486
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4496
NgoMIV G'CCGG_C
Cuts at: 665 2675 665
Size: 2010 2486
NspI r_CATG'y
Cuts at: 1549 2049 2956 1549
Size: 500 907 3089
Fragments arranged by size:
3089 907 500
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PflMI CCAn_nnn'nTGG
Cuts at: 1005 1469 1005
Size: 464 4032
PfoI T'CCnGG_A
Cuts at: 1217 1217
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 362 1151 362
Size: 789 3707
PstI C_TGCA'G
Cuts at: 1331 1543 2228 1331
Size: 212 685 3599
Fragments arranged by size:
3599 685 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1921 2281 1921
Size: 360 4136
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1510 1510
Size: 4496
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SanDI GG'GwC_CC
Cuts at: 362 1151 362
Size: 789 3707
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 1327 1539 2224 3217 3408 33
Size: 1294 212 685 993 191 1121
Fragments arranged by size:
1294 1121 993 685 212 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 676 751 769 1417 1651 2566 3058 3320 676
Size: 75 18 648 234 915 492 262 1852
Fragments arranged by size:
1852 915 648 492 262 234 75 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
StyI C'CwwG_G
Cuts at: 107 1019 1176 1463 1630 1984 107
Size: 912 157 287 167 354 2619
Fragments arranged by size:
2619 912 354 287 167 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 267 2079 2212 2671 2880 267
Size: 1812 133 459 209 1883
Fragments arranged by size:
1883 1812 459 209 133
TatI w'GTAC_w
Cuts at: 938 1364 4011 938
Size: 426 2647 1423
Fragments arranged by size:
2647 1423 426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4464
Tth111I GACn'n_nGTC
Cuts at: 625 2293 625
Size: 1668 2828
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1991 114
Size: 1877 2619
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
XmnI GAAnn'nnTTC
Cuts at: 232 232
Size: 4496
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI