Restriction map for
pF1KB8189
(Circular) (Six-base) MAPSORT of: pF1KB8189.seq Check: 2075 from: 1 to: 4451
pF1KB8189 4451 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1489 1489
Size: 4451
Acc65I G'GTAC_C
Cuts at: 1467 1467
Size: 4451
AclI AA'CG_TT
Cuts at: 4291 4291
Size: 4451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 728 801 885 969 1053 1137 1305 2276
Size: 291 73 84 84 84 84 168 971
Cuts at: 2276 2708 3455 3723 437
Size: 432 747 268 1165
Fragments arranged by size:
1165 971 747 432 291 268 168 84
84 84 84 73
AflIII A'CryG_T
Cuts at: 1803 2907 3884 1803
Size: 1104 977 2370
Fragments arranged by size:
2370 1104 977
AgeI A'CCGG_T
Cuts at: 2839 2839
Size: 4451
AleI CACnn'nnGTG
Cuts at: 1028 1028
Size: 4451
AlwNI CAG_nnn'CTG
Cuts at: 150 255 1756 3323 150
Size: 105 1501 1567 1278
Fragments arranged by size:
1567 1501 1278 105
ApaLI G'TGCA_C
Cuts at: 3221 3221
Size: 4451
ApoI r'AATT_y
Cuts at: 410 657 1355 1455 3804 4060 410
Size: 247 698 100 2349 256 801
Fragments arranged by size:
2349 801 698 256 247 100
AseI AT'TA_AT
Cuts at: 20 20
Size: 4451
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
AvaI C'yCGr_G
Cuts at: 1471 1471
Size: 4451
BamHI G'GATC_C
Cuts at: 935 1476 935
Size: 541 3910
BanI G'GyrC_C
Cuts at: 1467 1881 2129 2164 1467
Size: 414 248 35 3754
Fragments arranged by size:
3754 414 248 35
BanII G_rGCy'C
Cuts at: 829 997 1081 1165 1465 2495 829
Size: 168 84 84 300 1030 2785
Fragments arranged by size:
2785 1030 300 168 84 84
BbeI G_GCGC'C
Cuts at: 1885 2133 1885
Size: 248 4203
BbsI GAAGACnn'nnnn_
Cuts at: 425 985 425
Size: 560 3891
BciVI GTATCCnnnnn_n'
Cuts at: 2349 3110 4451 2349
Size: 761 1341 2349
Fragments arranged by size:
2349 1341 761
BclI T'GATC_A
Cuts at: 402 1971 402
Size: 1569 2882
BglII A'GATC_T
Cuts at: 1966 4003 1966
Size: 2037 2414
BlpI GC'TnA_GC
Cuts at: 1563 1563
Size: 4451
Bme1580I G_kGCm'C
Cuts at: 2076 2169 3225 2076
Size: 93 1056 3302
Fragments arranged by size:
3302 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 454 785 869 1663 1744 2079 4223 454
Size: 331 84 794 81 335 2144 682
Fragments arranged by size:
2144 794 682 335 331 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 4451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 560 1011 1133 1217 1385 370
Size: 190 451 122 84 168 3436
Fragments arranged by size:
3436 451 190 168 122 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1627 2506 2998 3296 463
Size: 1164 879 492 298 1618
Fragments arranged by size:
1618 1164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 379 481 4155 379
Size: 102 3674 675
Fragments arranged by size:
3674 675 102
BsaAI yAC'GTr
Cuts at: 1804 1804
Size: 4451
BsaBI GATnn'nnATC
Cuts at: 6 1965 4002 6
Size: 1959 2037 455
Fragments arranged by size:
2037 1959 455
BsaHI Gr'CG_yC
Cuts at: 1882 2130 2832 4328 1882
Size: 248 702 1496 2005
Fragments arranged by size:
2005 1496 702 248
BsaWI w'CCGG_w
Cuts at: 290 1640 2161 2839 3113 3260 3671 290
Size: 1350 521 678 274 147 411 1070
Fragments arranged by size:
1350 1070 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 604 604
Size: 4451
BseYI C'CCAG_C
Cuts at: 525 1876 2634 3211 3707 525
Size: 1351 758 577 496 1269
Fragments arranged by size:
1351 1269 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1421 1993 2039 3247 104
Size: 1317 572 46 1208 1308
Fragments arranged by size:
1317 1308 1208 572 46
BsiHKAI G_wGCw'C
Cuts at: 165 829 997 1081 1165 1465 2243 2433 3225
Size: 664 168 84 84 300 778 190 792
Cuts at: 3225 165
Size: 1391
Fragments arranged by size:
1391 792 778 664 300 190 168 84
84
Bsp1286I G_dGCh'C
Cuts at: 165 829 997 1081 1165 1465 2076 2169 2243
Size: 664 168 84 84 300 611 93 74
Cuts at: 2243 2433 2495 3225 165
Size: 190 62 730 1391
Fragments arranged by size:
1391 730 664 611 300 190 168 93
84 84 74 62
BspEI T'CCGG_A
Cuts at: 290 3671 290
Size: 3381 1070
BspHI T'CATG_A
Cuts at: 3627 3627
Size: 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 324 1501 1715 2017 2398 324
Size: 1177 214 302 381 2377
Fragments arranged by size:
2377 1177 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2743 2797 4281 4446 50
Size: 2693 54 1484 165 55
Fragments arranged by size:
2693 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1056 2363 1056
Size: 1307 3144
BsrFI r'CCGG_y
Cuts at: 2449 2630 2839 2449
Size: 181 209 4061
Fragments arranged by size:
4061 209 181
BssSI C'ACGA_G
Cuts at: 2722 3080 2722
Size: 358 4093
BstAPI GCAn_nnn'nTGC
Cuts at: 1828 1828
Size: 4451
BstBI TT'CG_AA
Cuts at: 2812 2812
Size: 4451
BstEII G'GTnAC_C
Cuts at: 258 258
Size: 4451
BstYI r'GATC_y
Cuts at: 1 935 1476 1943 1966 2301 2547 3559 3645
Size: 934 541 467 23 335 246 1012 86
Cuts at: 3645 3657 4003 1
Size: 12 346 449
Fragments arranged by size:
1012 934 541 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 143 143
Size: 4451
BtgI C'CryG_G
Cuts at: 107 689 1442 107
Size: 582 753 3116
Fragments arranged by size:
3116 753 582
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2401 2582 2745 2401
Size: 181 163 4107
Fragments arranged by size:
4107 181 163
BtsI GCAGTG_nn'
Cuts at: 1694 1694
Size: 4451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1038 1073 1038
Size: 35 4416
DraI TTT'AAA
Cuts at: 486 1450 486
Size: 964 3487
DrdI GACnn_nn'nnGTC
Cuts at: 2157 3015 2157
Size: 858 3593
EaeI y'GGCC_r
Cuts at: 2036 2210 2601 2628 3973 2036
Size: 174 391 27 1345 2514
Fragments arranged by size:
2514 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2036 2036
Size: 4451
EarI CTCTTCn'nnn_
Cuts at: 170 2474 2684 170
Size: 2304 210 1937
Fragments arranged by size:
2304 1937 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2659 2969 3115 4261 2659
Size: 310 146 1146 2849
Fragments arranged by size:
2849 1146 310 146
EcoICRI GAG'CTC
Cuts at: 827 995 1079 1163 1463 827
Size: 168 84 84 300 3815
Fragments arranged by size:
3815 300 168 84 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 437 560 728 801 885 969 1011 1053
Size: 67 123 168 73 84 84 42 42
Cuts at: 1053 1133 1137 1217 1305 1385 2276 2708 3455
Size: 80 4 80 88 80 891 432 747
Cuts at: 3455 3723 370
Size: 268 1098
Fragments arranged by size:
1098 891 747 432 268 168 123 88
84 84 80 80 80 73 67 42
42 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1071 1071
Size: 4451
EcoO109I rG'GnC_Cy
Cuts at: 147 327 438 1590 147
Size: 180 111 1152 3008
Fragments arranged by size:
3008 1152 180 111
EcoRI G'AATT_C
Cuts at: 657 1355 1455 657
Size: 698 100 3653
Fragments arranged by size:
3653 698 100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4451
FspI TGC'GCA
Cuts at: 2232 2232
Size: 4451
HaeII r_GCGC'y
Cuts at: 1885 2133 3155 4134 1885
Size: 248 1022 979 2202
Fragments arranged by size:
2202 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 412 444 720 752 2293 2323 2325
Size: 32 265 32 276 32 1541 30 2
Cuts at: 2325 2355 115
Size: 30 2211
Fragments arranged by size:
2211 1541 276 265 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1490 1490
Size: 4451
KasI G'GCGC_C
Cuts at: 1881 2129 1881
Size: 248 4203
KpnI G_GTAC'C
Cuts at: 1471 1471
Size: 4451
MluI A'CGCG_T
Cuts at: 3884 3884
Size: 4451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 215 686 3122 3306 215
Size: 471 2436 184 1360
Fragments arranged by size:
2436 1360 471 184
MscI TGG'CCA
Cuts at: 2212 2212
Size: 4451
MslI CAynn'nnrTG
Cuts at: 710 1028 2440 2567 710
Size: 318 1412 127 2594
Fragments arranged by size:
2594 1412 318 127
MspA1I CmG'CkG
Cuts at: 525 905 989 1562 1876 2236 3249 3494 525
Size: 380 84 573 314 360 1013 245 1482
Fragments arranged by size:
1482 1013 573 380 360 314 245 84
NaeI GCC'GGC
Cuts at: 2632 2632
Size: 4451
NarI GG'CG_CC
Cuts at: 1882 2130 1882
Size: 248 4203
NcoI C'CATG_G
Cuts at: 107 107
Size: 4451
NgoMIV G'CCGG_C
Cuts at: 2630 2630
Size: 4451
NspI r_CATG'y
Cuts at: 1504 2004 2911 1504
Size: 500 907 3044
Fragments arranged by size:
3044 907 500
PasI CC'CwG_GG
Cuts at: 605 605
Size: 4451
PciI A'CATG_T
Cuts at: 2907 2907
Size: 4451
PflMI CCAn_nnn'nTGG
Cuts at: 255 571 255
Size: 316 4135
PmeI GTTT'AAAC
Cuts at: 1450 1450
Size: 4451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4419
PpuMI rG'GwC_Cy
Cuts at: 327 438 327
Size: 111 4340
PstI C_TGCA'G
Cuts at: 1498 2183 1498
Size: 685 3766
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4451
PvuII CAG'CTG
Cuts at: 905 989 1876 2236 905
Size: 84 887 360 3120
Fragments arranged by size:
3120 887 360 84
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 4451
SacI G_AGCT'C
Cuts at: 829 997 1081 1165 1465 829
Size: 168 84 84 300 3815
Fragments arranged by size:
3815 300 168 84 84
SalI G'TCGA_C
Cuts at: 1488 1488
Size: 4451
SapI GCTCTTCn'nnn_
Cuts at: 170 2474 2684 170
Size: 2304 210 1937
Fragments arranged by size:
2304 1937 210
SbfI CC_TGCA'GG
Cuts at: 1498 1498
Size: 4451
ScaI AGT'ACT
Cuts at: 3968 3968
Size: 4451
SfcI C'TryA_G
Cuts at: 33 333 906 1242 1494 2179 3172 3363 33
Size: 300 573 336 252 685 993 191 1121
Fragments arranged by size:
1121 993 685 573 336 300 252 191
SfoI GGC'GCC
Cuts at: 1883 2131 1883
Size: 248 4203
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
SmaI CCC'GGG
Cuts at: 1473 1473
Size: 4451
SmlI C'TyrA_G
Cuts at: 478 1606 2521 3013 3275 478
Size: 1128 915 492 262 1654
Fragments arranged by size:
1654 1128 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4451
SphI G_CATG'C
Cuts at: 1504 2004 1504
Size: 500 3951
StuI AGG'CCT
Cuts at: 814 1066 1253 814
Size: 252 187 4012
Fragments arranged by size:
4012 252 187
StyI C'CwwG_G
Cuts at: 107 1585 1939 107
Size: 1478 354 2619
Fragments arranged by size:
2619 1478 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1021 1435 2034 2167 2626 2835 1021
Size: 414 599 133 459 209 2637
Fragments arranged by size:
2637 599 459 414 209 133
TatI w'GTAC_w
Cuts at: 281 3966 281
Size: 3685 766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 422 454 3968 4000 422
Size: 32 3514 32 873
Fragments arranged by size:
3514 873 32 32
Tth111I GACn'n_nGTC
Cuts at: 2248 2248
Size: 4451
XbaI T'CTAG_A
Cuts at: 1482 1482
Size: 4451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1946 1946
Size: 4451
XmaI C'CCGG_G
Cuts at: 1471 1471
Size: 4451
XmnI GAAnn'nnTTC
Cuts at: 367 646 814 898 982 1066 1150 1318 367
Size: 279 168 84 84 84 84 168 3500
Fragments arranged by size:
3500 279 168 168 84 84 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI