Restriction map for
pF1KB8191
(Circular) (Six-base) MAPSORT of: pF1KB8191.seq Check: 4335 from: 1 to: 4388
pF1KB8191 4388 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 324 324
Size: 4388
AccI GT'mk_AC
Cuts at: 1196 1426 1196
Size: 230 4158
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 2213 2645 3392 3660 1147
Size: 1066 432 747 268 1875
Fragments arranged by size:
1875 1066 747 432 268
AfeI AGC'GCT
Cuts at: 910 1041 1075 910
Size: 131 34 4223
Fragments arranged by size:
4223 131 34
AflIII A'CryG_T
Cuts at: 1740 2844 3821 1740
Size: 1104 977 2307
Fragments arranged by size:
2307 1104 977
AgeI A'CCGG_T
Cuts at: 2776 2776
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 1693 3260 1693
Size: 1567 2821
ApaLI G'TGCA_C
Cuts at: 1215 3158 1215
Size: 1943 2445
ApoI r'AATT_y
Cuts at: 1392 3741 3997 1392
Size: 2349 256 1783
Fragments arranged by size:
2349 1783 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 1408 1408
Size: 4388
AvrII C'CTAG_G
Cuts at: 918 918
Size: 4388
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 525 1085 1404 1818 2066 2101 525
Size: 560 319 414 248 35 2812
Fragments arranged by size:
2812 560 414 319 248 35
BanII G_rGCy'C
Cuts at: 356 623 1354 1402 2432 356
Size: 267 731 48 1030 2312
Fragments arranged by size:
2312 1030 731 267 48
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbsI GAAGACnn'nnnn_
Cuts at: 244 1008 244
Size: 764 3624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1215 1249 1215
Size: 34 4354
BciVI GTATCCnnnnn_n'
Cuts at: 2286 3047 4388 2286
Size: 761 1341 2286
Fragments arranged by size:
2286 1341 761
BclI T'GATC_A
Cuts at: 1908 1908
Size: 4388
BglI GCCn_nnn'nGGC
Cuts at: 155 1162 155
Size: 1007 3381
BglII A'GATC_T
Cuts at: 1130 1903 3940 1130
Size: 773 2037 1578
Fragments arranged by size:
2037 1578 773
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 1219 2013 2106 3162 1219
Size: 794 93 1056 2445
Fragments arranged by size:
2445 1056 794 93
BmrI ACTGGGnnnn_n'
Cuts at: 1161 1600 1681 2016 4160 1161
Size: 439 81 335 2144 1389
Fragments arranged by size:
2144 1389 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 280
Size: 4388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1564 2443 2935 3233 217
Size: 1347 879 492 298 1372
Fragments arranged by size:
1372 1347 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 634 4092 634
Size: 3458 930
BsaAI yAC'GTr
Cuts at: 1741 1741
Size: 4388
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3050 3197 3608 1577
Size: 521 678 274 147 411 2357
Fragments arranged by size:
2357 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 211 998 1028 181
Size: 30 787 30 3541
Fragments arranged by size:
3541 787 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 202
Size: 4388
BseYI C'CCAG_C
Cuts at: 153 747 1244 1813 2571 3148 3644 153
Size: 594 497 569 758 577 496 897
Fragments arranged by size:
897 758 594 577 569 497 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1285 382
Size: 903 3485
BsiEI CG_ry'CG
Cuts at: 104 140 551 1930 1976 3184 104
Size: 36 411 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 411 46 36
BsiHKAI G_wGCw'C
Cuts at: 808 1219 1402 2180 2370 3162 808
Size: 411 183 778 190 792 2034
Fragments arranged by size:
2034 792 778 411 190 183
BsmI GAATG_Cn'
Cuts at: 951 951
Size: 4388
Bsp1286I G_dGCh'C
Cuts at: 356 623 808 1219 1354 1402 2013 2106 2180
Size: 267 185 411 135 48 611 93 74
Cuts at: 2180 2370 2432 3162 356
Size: 190 62 730 1582
Fragments arranged by size:
1582 730 611 411 267 190 185 135
93 74 62 48
BspEI T'CCGG_A
Cuts at: 3608 3608
Size: 4388
BspHI T'CATG_A
Cuts at: 3564 3564
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 324 404 703 776 1438 1652 1954 2335 324
Size: 80 299 73 662 214 302 381 2377
Fragments arranged by size:
2377 662 381 302 299 214 80 73
BsrBI CCG'CTC
Cuts at: 50 1252 2680 2734 4218 4383 50
Size: 1202 1428 54 1484 165 55
Fragments arranged by size:
1484 1428 1202 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 326 792 2386 2567 2776 326
Size: 466 1594 181 209 1938
Fragments arranged by size:
1938 1594 466 209 181
BssSI C'ACGA_G
Cuts at: 409 2659 3017 409
Size: 2250 358 1780
Fragments arranged by size:
2250 1780 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1765 1765
Size: 4388
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstXI CCAn_nnnn'nTGG
Cuts at: 318 876 1078 318
Size: 558 202 3628
Fragments arranged by size:
3628 558 202
BstYI r'GATC_y
Cuts at: 1 542 1106 1130 1413 1880 1903 2238 2484
Size: 541 564 24 283 467 23 335 246
Cuts at: 2484 3496 3582 3594 3940 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 564 541 467 449 346 335 283
246 86 24 23 12
BtgI C'CryG_G
Cuts at: 422 422
Size: 4388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 399 2338 2519 2682 399
Size: 1939 181 163 2105
Fragments arranged by size:
2105 1939 181 163
BtsI GCAGTG_nn'
Cuts at: 959 1327 1631 959
Size: 368 304 3716
Fragments arranged by size:
3716 368 304
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DraIII CAC_nnn'GTG
Cuts at: 296 296
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 324 388 548 1091 1973 2147 2538 2565 3910
Size: 64 160 543 882 174 391 27 1345
Cuts at: 3910 324
Size: 802
Fragments arranged by size:
1345 882 802 543 391 174 160 64
27
EagI C'GGCC_G
Cuts at: 548 1973 548
Size: 1425 2963
EarI CTCTTCn'nnn_
Cuts at: 975 2411 2621 975
Size: 1436 210 2742
Fragments arranged by size:
2742 1436 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2596 2906 3052 4198 2596
Size: 310 146 1146 2786
Fragments arranged by size:
2786 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 1147 2213 2645 3392 3660 280
Size: 867 1066 432 747 268 1008
Fragments arranged by size:
1066 1008 867 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 314 1335 1527 314
Size: 1021 192 3175
Fragments arranged by size:
3175 1021 192
EcoRI G'AATT_C
Cuts at: 1392 1392
Size: 4388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 150 912 1043 1077 1822 2070 3092 4071 150
Size: 762 131 34 745 248 1022 979 467
Fragments arranged by size:
1022 979 762 745 467 248 131 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 998 1030 2230 2260 2262 2292 998
Size: 32 1200 30 2 30 3094
Fragments arranged by size:
3094 1200 32 30 30 2
HincII GTy'rAC
Cuts at: 288 939 1176 1205 1427 288
Size: 651 237 29 222 3249
Fragments arranged by size:
3249 651 237 222 29
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3059 3243 3059
Size: 184 4204
MscI TGG'CCA
Cuts at: 390 2149 390
Size: 1759 2629
MslI CAynn'nnrTG
Cuts at: 874 1116 1225 1307 1345 2377 2504 874
Size: 242 109 82 38 1032 127 2758
Fragments arranged by size:
2758 1032 242 127 109 82 38
MspA1I CmG'CkG
Cuts at: 249 1367 1499 1813 2173 3186 3431 249
Size: 1118 132 314 360 1013 245 1206
Fragments arranged by size:
1206 1118 1013 360 314 245 132
NaeI GCC'GGC
Cuts at: 794 2569 794
Size: 1775 2613
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 422 422
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 792 2567 792
Size: 1775 2613
NspI r_CATG'y
Cuts at: 1441 1941 2848 1441
Size: 500 907 2981
Fragments arranged by size:
2981 907 500
PciI A'CATG_T
Cuts at: 2844 2844
Size: 4388
PflMI CCAn_nnn'nTGG
Cuts at: 652 705 652
Size: 53 4335
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 4356
PpuMI rG'GwC_Cy
Cuts at: 314 1335 314
Size: 1021 3367
PstI C_TGCA'G
Cuts at: 773 1435 2120 773
Size: 662 685 3041
Fragments arranged by size:
3041 685 662
PvuI CG_AT'CG
Cuts at: 104 140 104
Size: 36 4352
PvuII CAG'CTG
Cuts at: 249 1813 2173 249
Size: 1564 360 2464
Fragments arranged by size:
2464 1564 360
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 2411 2621 2411
Size: 210 4178
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 573 769 1431 2116 3109 3300 33
Size: 540 196 662 685 993 191 1121
Fragments arranged by size:
1121 993 685 662 540 196 191
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 1410 1410
Size: 4388
SmlI C'TyrA_G
Cuts at: 232 1543 2458 2950 3212 232
Size: 1311 915 492 262 1408
Fragments arranged by size:
1408 1311 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
SspI AAT'ATT
Cuts at: 633 633
Size: 4388
StyI C'CwwG_G
Cuts at: 422 918 1522 1876 422
Size: 496 604 354 2934
Fragments arranged by size:
2934 604 496 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1161 1357 1971 2104 2563 2772 1161
Size: 196 614 133 459 209 2777
Fragments arranged by size:
2777 614 459 209 196 133
TatI w'GTAC_w
Cuts at: 1256 3903 1256
Size: 2647 1741
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 971 1003 3905 3937 181
Size: 32 758 32 2902 32 632
Fragments arranged by size:
2902 758 632 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 463 1333 2185 463
Size: 870 852 2666
Fragments arranged by size:
2666 870 852
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1883 1883
Size: 4388
XmaI C'CCGG_G
Cuts at: 1408 1408
Size: 4388
XmnI GAAnn'nnTTC
Cuts at: 1132 1132
Size: 4388
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI