Restriction map for
pF1KB8198
(Circular) (Six-base) MAPSORT of: pF1KB8198.seq Check: 7749 from: 1 to: 3716
pF1KB8198 3716 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 377 377
Size: 3716
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1541 1973 2720 2988 268
Size: 1273 432 747 268 996
Fragments arranged by size:
1273 996 747 432 268
AflIII A'CryG_T
Cuts at: 200 1068 2172 3149 200
Size: 868 1104 977 767
Fragments arranged by size:
1104 977 868 767
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AlwNI CAG_nnn'CTG
Cuts at: 414 643 1021 2588 414
Size: 229 378 1567 1542
Fragments arranged by size:
1567 1542 378 229
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 720 3069 3325 720
Size: 2349 256 1111
Fragments arranged by size:
2349 1111 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 736 736
Size: 3716
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 732 1146 1394 1429 732
Size: 414 248 35 3019
Fragments arranged by size:
3019 414 248 35
BanII G_rGCy'C
Cuts at: 351 730 1760 351
Size: 379 1030 2307
Fragments arranged by size:
2307 1030 379
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglII A'GATC_T
Cuts at: 287 1231 3268 287
Size: 944 2037 735
Fragments arranged by size:
2037 944 735
BlpI GC'TnA_GC
Cuts at: 828 828
Size: 3716
Bme1580I G_kGCm'C
Cuts at: 1341 1434 2490 1341
Size: 93 1056 2567
Fragments arranged by size:
2567 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 928 1009 1344 3488 928
Size: 81 335 2144 1156
Fragments arranged by size:
2144 1156 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 653
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 892 1771 2263 2561 649
Size: 243 879 492 298 1804
Fragments arranged by size:
1804 879 492 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 3716
BsaAI yAC'GTr
Cuts at: 203 1069 203
Size: 866 2850
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 374 1147 1395 2097 3593 374
Size: 773 248 702 1496 497
Fragments arranged by size:
1496 773 702 497 248
BsaWI w'CCGG_w
Cuts at: 317 905 1426 2104 2378 2525 2936 317
Size: 588 521 678 274 147 411 1097
Fragments arranged by size:
1097 678 588 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1141 1899 2476 2972 1141
Size: 758 577 496 1885
Fragments arranged by size:
1885 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1258 1304 2512 104
Size: 1154 46 1208 1308
Fragments arranged by size:
1308 1208 1154 46
BsiHKAI G_wGCw'C
Cuts at: 730 1508 1698 2490 730
Size: 778 190 792 1956
Fragments arranged by size:
1956 792 778 190
BsmI GAATG_Cn'
Cuts at: 573 573
Size: 3716
BsmBI CGTCTCn'nnnn_
Cuts at: 547 547
Size: 3716
Bsp1286I G_dGCh'C
Cuts at: 351 730 1341 1434 1508 1698 1760 2490 351
Size: 379 611 93 74 190 62 730 1577
Fragments arranged by size:
1577 730 611 379 190 93 74 62
BspEI T'CCGG_A
Cuts at: 317 2936 317
Size: 2619 1097
BspHI T'CATG_A
Cuts at: 593 2892 593
Size: 2299 1417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 980 1282 1663 766
Size: 214 302 381 2819
Fragments arranged by size:
2819 381 302 214
BsrBI CCG'CTC
Cuts at: 50 654 2008 2062 3546 3711 50
Size: 604 1354 54 1484 165 55
Fragments arranged by size:
1484 1354 604 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 690 1714 1895 2104 690
Size: 1024 181 209 2302
Fragments arranged by size:
2302 1024 209 181
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 173 414 1093 173
Size: 241 679 2796
Fragments arranged by size:
2796 679 241
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstXI CCAn_nnnn'nTGG
Cuts at: 234 261 495 234
Size: 27 234 3455
Fragments arranged by size:
3455 234 27
BstYI r'GATC_y
Cuts at: 1 245 287 741 1208 1231 1566 1812 2824
Size: 244 42 454 467 23 335 246 1012
Cuts at: 2824 2910 2922 3268 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 454 449 346 335 246 244
86 42 23 12
BtgI C'CryG_G
Cuts at: 599 599
Size: 3716
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 415 959 415
Size: 544 3172
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2280 1422
Size: 858 2858
EaeI y'GGCC_r
Cuts at: 1301 1475 1866 1893 3238 1301
Size: 174 391 27 1345 1779
Fragments arranged by size:
1779 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1301 1301
Size: 3716
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 653 1541 1973 2720 2988 268
Size: 385 888 432 747 268 996
Fragments arranged by size:
996 888 747 432 385 268
EcoO109I rG'GnC_Cy
Cuts at: 628 855 628
Size: 227 3489
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1735 1735
Size: 3716
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 563 1150 1398 2420 3399 563
Size: 587 248 1022 979 880
Fragments arranged by size:
1022 979 880 587 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 1558 1588 1590 1620 373
Size: 32 1153 30 2 30 2469
Fragments arranged by size:
2469 1153 32 30 30 2
HincII GTy'rAC
Cuts at: 755 755
Size: 3716
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2571 2387
Size: 184 3532
MscI TGG'CCA
Cuts at: 1477 1477
Size: 3716
MslI CAynn'nnrTG
Cuts at: 232 361 364 598 1705 1832 232
Size: 129 3 234 1107 127 2116
Fragments arranged by size:
2116 1107 234 129 127 3
MspA1I CmG'CkG
Cuts at: 301 827 1141 1501 2514 2759 301
Size: 526 314 360 1013 245 1258
Fragments arranged by size:
1258 1013 526 360 314 245
NaeI GCC'GGC
Cuts at: 692 1897 692
Size: 1205 2511
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NcoI C'CATG_G
Cuts at: 599 599
Size: 3716
NgoMIV G'CCGG_C
Cuts at: 690 1895 690
Size: 1205 2511
NspI r_CATG'y
Cuts at: 769 1269 2176 769
Size: 500 907 2309
Fragments arranged by size:
2309 907 500
PciI A'CATG_T
Cuts at: 2172 2172
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 574 606 2855 2887 574
Size: 32 2249 32 1403
Fragments arranged by size:
2249 1403 32 32
PstI C_TGCA'G
Cuts at: 419 763 1448 419
Size: 344 685 2687
Fragments arranged by size:
2687 685 344
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 1141 1501 1141
Size: 360 3356
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 730 730
Size: 3716
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SfcI C'TryA_G
Cuts at: 33 415 759 1444 2437 2628 33
Size: 382 344 685 993 191 1121
Fragments arranged by size:
1121 993 685 382 344 191
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 738 738
Size: 3716
SmlI C'TyrA_G
Cuts at: 664 871 1786 2278 2540 664
Size: 207 915 492 262 1840
Fragments arranged by size:
1840 915 492 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
StyI C'CwwG_G
Cuts at: 515 599 850 1204 515
Size: 84 251 354 3027
Fragments arranged by size:
3027 354 251 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1299 1432 1891 2100 1299
Size: 133 459 209 2915
Fragments arranged by size:
2915 459 209 133
TatI w'GTAC_w
Cuts at: 3231 3231
Size: 3716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 3684
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 492 495 1211 492
Size: 3 716 2997
Fragments arranged by size:
2997 716 3
XmaI C'CCGG_G
Cuts at: 736 736
Size: 3716
ZraI GAC'GTC
Cuts at: 375 375
Size: 3716
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI