Restriction map for
pF1KB8200
(Circular) (Six-base) MAPSORT of: pF1KB8200.seq Check: 1006 from: 1 to: 4229
pF1KB8200 4229 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 258 258
Size: 4229
AccI GT'mk_AC
Cuts at: 1267 1267
Size: 4229
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 2054 2486 3233 3501 370
Size: 1684 432 747 268 1098
Fragments arranged by size:
1684 1098 747 432 268
AfeI AGC'GCT
Cuts at: 1009 1009
Size: 4229
AflIII A'CryG_T
Cuts at: 338 1581 2685 3662 338
Size: 1243 1104 977 905
Fragments arranged by size:
1243 1104 977 905
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 676 708 676
Size: 32 4197
AlwNI CAG_nnn'CTG
Cuts at: 710 1020 1534 3101 710
Size: 310 514 1567 1838
Fragments arranged by size:
1838 1567 514 310
ApaI G_GGCC'C
Cuts at: 291 423 291
Size: 132 4097
ApaLI G'TGCA_C
Cuts at: 241 2999 241
Size: 2758 1471
ApoI r'AATT_y
Cuts at: 1233 3582 3838 1233
Size: 2349 256 1624
Fragments arranged by size:
2349 1624 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 292 1249 292
Size: 957 3272
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 223 557 648 1245 1659 1907 1942 223
Size: 334 91 597 414 248 35 2510
Fragments arranged by size:
2510 597 414 334 248 91 35
BanII G_rGCy'C
Cuts at: 137 251 291 423 667 982 1039 1243 2273
Size: 114 40 132 244 315 57 204 1030
Cuts at: 2273 137
Size: 2093
Fragments arranged by size:
2093 1030 315 244 204 132 114 57
40
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3981
BbvCI CC'TCA_GC
Cuts at: 544 544
Size: 4229
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4229
BglII A'GATC_T
Cuts at: 1100 1744 3781 1100
Size: 644 2037 1548
Fragments arranged by size:
2037 1548 644
BlpI GC'TnA_GC
Cuts at: 943 1111 1341 943
Size: 168 230 3831
Fragments arranged by size:
3831 230 168
Bme1580I G_kGCm'C
Cuts at: 245 291 423 1854 1947 3003 245
Size: 46 132 1431 93 1056 1471
Fragments arranged by size:
1471 1431 1056 132 93 46
BmrI ACTGGGnnnn_n'
Cuts at: 294 1441 1522 1857 4001 294
Size: 1147 81 335 2144 522
Fragments arranged by size:
2144 1147 522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
Bpu10I CC'TnA_GC
Cuts at: 544 971 544
Size: 427 3802
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1198 1405 2284 2776 3074 235
Size: 963 207 879 492 298 1390
Fragments arranged by size:
1390 963 879 492 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 3933 3933
Size: 4229
BsaAI yAC'GTr
Cuts at: 339 1582 339
Size: 1243 2986
BsaBI GATnn'nnATC
Cuts at: 6 178 469 1743 3780 6
Size: 172 291 1274 2037 455
Fragments arranged by size:
2037 1274 455 291 172
BsaHI Gr'CG_yC
Cuts at: 728 926 1660 1908 2610 4106 728
Size: 198 734 248 702 1496 851
Fragments arranged by size:
1496 851 734 702 248 198
BsaWI w'CCGG_w
Cuts at: 1418 1939 2617 2891 3038 3449 1418
Size: 521 678 274 147 411 2198
Fragments arranged by size:
2198 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 416 716 1654 2412 2989 3485 416
Size: 300 938 758 577 496 1160
Fragments arranged by size:
1160 938 758 577 496 300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1128 1063
Size: 65 4164
BsiEI CG_ry'CG
Cuts at: 104 646 1771 1817 3025 104
Size: 542 1125 46 1208 1308
Fragments arranged by size:
1308 1208 1125 542 46
BsiHKAI G_wGCw'C
Cuts at: 245 251 260 982 1039 1112 1135 1243 2021
Size: 6 9 722 57 73 23 108 778
Cuts at: 2021 2211 3003 245
Size: 190 792 1471
Fragments arranged by size:
1471 792 778 722 190 108 73 57
23 9 6
Bsp1286I G_dGCh'C
Cuts at: 137 245 251 260 291 423 667 982 1039
Size: 108 6 9 31 132 244 315 57
Cuts at: 1039 1112 1135 1243 1854 1947 2021 2211 2273
Size: 73 23 108 611 93 74 190 62
Cuts at: 2273 3003 137
Size: 730 1363
Fragments arranged by size:
1363 730 611 315 244 190 132 108
108 93 74 73 62 57 31 23
9 6
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 258 322 1279 1493 1795 2176 258
Size: 64 957 214 302 381 2311
Fragments arranged by size:
2311 957 381 302 214 64
BsrBI CCG'CTC
Cuts at: 50 221 533 2521 2575 4059 4224 50
Size: 171 312 1988 54 1484 165 55
Fragments arranged by size:
1988 1484 312 171 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2141
Size: 4229
BsrFI r'CCGG_y
Cuts at: 583 645 2227 2408 2617 583
Size: 62 1582 181 209 2195
Fragments arranged by size:
2195 1582 209 181 62
BssSI C'ACGA_G
Cuts at: 244 458 2500 2858 244
Size: 214 2042 358 1615
Fragments arranged by size:
2042 1615 358 214
BstAPI GCAn_nnn'nTGC
Cuts at: 710 785 1606 710
Size: 75 821 3333
Fragments arranged by size:
3333 821 75
BstBI TT'CG_AA
Cuts at: 695 2590 695
Size: 1895 2334
BstXI CCAn_nnnn'nTGG
Cuts at: 1155 1155
Size: 4229
BstYI r'GATC_y
Cuts at: 1 608 1100 1254 1721 1744 2079 2325 3337
Size: 607 492 154 467 23 335 246 1012
Cuts at: 3337 3423 3435 3781 1
Size: 86 12 346 449
Fragments arranged by size:
1012 607 492 467 449 346 335 246
154 86 23 12
BtgI C'CryG_G
Cuts at: 107 658 932 107
Size: 551 274 3404
Fragments arranged by size:
3404 551 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3885
Fragments arranged by size:
3885 181 163
BtsI GCAGTG_nn'
Cuts at: 844 1472 844
Size: 628 3601
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DraIII CAC_nnn'GTG
Cuts at: 659 659
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 781 1935 2793 781
Size: 1154 858 2217
Fragments arranged by size:
2217 1154 858
EaeI y'GGCC_r
Cuts at: 643 812 1814 1988 2379 2406 3751 643
Size: 169 1002 174 391 27 1345 1121
Fragments arranged by size:
1345 1121 1002 391 174 169 27
EagI C'GGCC_G
Cuts at: 643 1814 643
Size: 1171 3058
EarI CTCTTCn'nnn_
Cuts at: 1180 2252 2462 1180
Size: 1072 210 2947
Fragments arranged by size:
2947 1072 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2437 2747 2893 4039 2437
Size: 310 146 1146 2627
Fragments arranged by size:
2627 1146 310 146
EcoICRI GAG'CTC
Cuts at: 249 980 1037 1241 249
Size: 731 57 204 3237
Fragments arranged by size:
3237 731 204 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 2054 2486 3233 3501 370
Size: 1684 432 747 268 1098
Fragments arranged by size:
1684 1098 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 288 420 1209 1368 288
Size: 132 789 159 3149
Fragments arranged by size:
3149 789 159 132
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 693 2248 693
Size: 1555 2674
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4229
HaeII r_GCGC'y
Cuts at: 856 1011 1663 1911 2933 3912 856
Size: 155 652 248 1022 979 1173
Fragments arranged by size:
1173 1022 979 652 248 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2071 2101 2103 2133 2071
Size: 30 2 30 4167
Fragments arranged by size:
4167 30 30 2
HincII GTy'rAC
Cuts at: 1268 1268
Size: 4229
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3981
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 586 2900 3084 586
Size: 2314 184 1731
Fragments arranged by size:
2314 1731 184
MscI TGG'CCA
Cuts at: 1990 1990
Size: 4229
MslI CAynn'nnrTG
Cuts at: 209 1153 2218 2345 209
Size: 944 1065 127 2093
Fragments arranged by size:
2093 1065 944 127
MspA1I CmG'CkG
Cuts at: 143 407 452 746 889 934 1115 1340 1654
Size: 264 45 294 143 45 181 225 314
Cuts at: 1654 2014 3027 3272 143
Size: 360 1013 245 1100
Fragments arranged by size:
1100 1013 360 314 294 264 245 225
181 143 45 45
NaeI GCC'GGC
Cuts at: 585 647 2410 585
Size: 62 1763 2404
Fragments arranged by size:
2404 1763 62
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3981
NcoI C'CATG_G
Cuts at: 107 107
Size: 4229
NgoMIV G'CCGG_C
Cuts at: 583 645 2408 583
Size: 62 1763 2404
Fragments arranged by size:
2404 1763 62
NruI TCG'CGA
Cuts at: 820 820
Size: 4229
NspI r_CATG'y
Cuts at: 1282 1782 2689 1282
Size: 500 907 2822
Fragments arranged by size:
2822 907 500
PciI A'CATG_T
Cuts at: 2685 2685
Size: 4229
PflMI CCAn_nnn'nTGG
Cuts at: 659 1020 659
Size: 361 3868
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 4197
PpuMI rG'GwC_Cy
Cuts at: 1209 1209
Size: 4229
PspOMI G'GGCC_C
Cuts at: 287 419 287
Size: 132 4097
PstI C_TGCA'G
Cuts at: 1276 1961 1276
Size: 685 3544
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 143 1654 2014 143
Size: 1511 360 2358
Fragments arranged by size:
2358 1511 360
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 251 982 1039 1243 251
Size: 731 57 204 3237
Fragments arranged by size:
3237 731 204 57
SacII CC_GC'GG
Cuts at: 935 935
Size: 4229
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 1180 2252 2462 1180
Size: 1072 210 2947
Fragments arranged by size:
2947 1072 210
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SfcI C'TryA_G
Cuts at: 33 1272 1957 2950 3141 33
Size: 1239 685 993 191 1121
Fragments arranged by size:
1239 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3981
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 250 1213 1384 2299 2791 3053 250
Size: 963 171 915 492 262 1426
Fragments arranged by size:
1426 963 915 492 262 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3729
StyI C'CwwG_G
Cuts at: 107 236 1363 1717 107
Size: 129 1127 354 2619
Fragments arranged by size:
2619 1127 354 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 184 666 1812 1945 2404 2613 184
Size: 482 1146 133 459 209 1800
Fragments arranged by size:
1800 1146 482 459 209 133
TatI w'GTAC_w
Cuts at: 3744 3744
Size: 4229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4197
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4229
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1724 1724
Size: 4229
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI AvrII BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI