Restriction map for
pF1KB8201
(Circular) (Six-base) MAPSORT of: pF1KB8201.seq Check: 8403 from: 1 to: 3722
pF1KB8201 3722 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 368 760 368
Size: 392 3330
Acc65I G'GTAC_C
Cuts at: 738 738
Size: 3722
AclI AA'CG_TT
Cuts at: 3562 3562
Size: 3722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1979 2726 2994 1547
Size: 432 747 268 2275
Fragments arranged by size:
2275 747 432 268
AflIII A'CryG_T
Cuts at: 1074 2178 3155 1074
Size: 1104 977 1641
Fragments arranged by size:
1641 1104 977
AgeI A'CCGG_T
Cuts at: 2110 2110
Size: 3722
AlwNI CAG_nnn'CTG
Cuts at: 1027 2594 1027
Size: 1567 2155
ApaLI G'TGCA_C
Cuts at: 2492 2492
Size: 3722
ApoI r'AATT_y
Cuts at: 726 3075 3331 726
Size: 2349 256 1117
Fragments arranged by size:
2349 1117 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 156 527 605 742 156
Size: 371 78 137 3136
Fragments arranged by size:
3136 371 137 78
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 738 1152 1400 1435 738
Size: 414 248 35 3025
Fragments arranged by size:
3025 414 248 35
BanII G_rGCy'C
Cuts at: 736 1766 736
Size: 1030 2692
BbeI G_GCGC'C
Cuts at: 1156 1404 1156
Size: 248 3474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 541 575 541
Size: 34 3688
BciVI GTATCCnnnnn_n'
Cuts at: 1620 2381 3722 1620
Size: 761 1341 1620
Fragments arranged by size:
1620 1341 761
BclI T'GATC_A
Cuts at: 179 1242 179
Size: 1063 2659
BglII A'GATC_T
Cuts at: 1237 3274 1237
Size: 2037 1685
BlpI GC'TnA_GC
Cuts at: 834 834
Size: 3722
Bme1580I G_kGCm'C
Cuts at: 1347 1440 2496 1347
Size: 93 1056 2573
Fragments arranged by size:
2573 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 656 934 1015 1350 3494 656
Size: 278 81 335 2144 884
Fragments arranged by size:
2144 884 335 278 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 453 2089 453
Size: 1636 2086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 898 1777 2269 2567 604
Size: 294 879 492 298 1759
Fragments arranged by size:
1759 879 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 3426 3426
Size: 3722
BsaAI yAC'GTr
Cuts at: 1075 1075
Size: 3722
BsaBI GATnn'nnATC
Cuts at: 6 1236 3273 6
Size: 1230 2037 455
Fragments arranged by size:
2037 1230 455
BsaHI Gr'CG_yC
Cuts at: 1153 1401 2103 3599 1153
Size: 248 702 1496 1276
Fragments arranged by size:
1496 1276 702 248
BsaWI w'CCGG_w
Cuts at: 911 1432 2110 2384 2531 2942 911
Size: 521 678 274 147 411 1691
Fragments arranged by size:
1691 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 691 721 691
Size: 30 3692
BseYI C'CCAG_C
Cuts at: 442 462 1147 1905 2482 2978 442
Size: 20 685 758 577 496 1186
Fragments arranged by size:
1186 758 685 577 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 520
Size: 3722
BsiEI CG_ry'CG
Cuts at: 104 1264 1310 2518 104
Size: 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 46
BsiHKAI G_wGCw'C
Cuts at: 709 736 1514 1704 2496 709
Size: 27 778 190 792 1935
Fragments arranged by size:
1935 792 778 190 27
BsmI GAATG_Cn'
Cuts at: 438 438
Size: 3722
Bsp1286I G_dGCh'C
Cuts at: 709 736 1347 1440 1514 1704 1766 2496 709
Size: 27 611 93 74 190 62 730 1935
Fragments arranged by size:
1935 730 611 190 93 74 62 27
BspEI T'CCGG_A
Cuts at: 2942 2942
Size: 3722
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 986 1288 1669 772
Size: 214 302 381 2825
Fragments arranged by size:
2825 381 302 214
BsrBI CCG'CTC
Cuts at: 50 312 2014 2068 3552 3717 50
Size: 262 1702 54 1484 165 55
Fragments arranged by size:
1702 1484 262 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1634 1634
Size: 3722
BsrFI r'CCGG_y
Cuts at: 1720 1901 2110 1720
Size: 181 209 3332
Fragments arranged by size:
3332 209 181
BssSI C'ACGA_G
Cuts at: 1993 2351 1993
Size: 358 3364
BstAPI GCAn_nnn'nTGC
Cuts at: 1099 1099
Size: 3722
BstBI TT'CG_AA
Cuts at: 551 2083 551
Size: 1532 2190
BstYI r'GATC_y
Cuts at: 1 245 747 1214 1237 1572 1818 2830 2916
Size: 244 502 467 23 335 246 1012 86
Cuts at: 2916 2928 3274 1
Size: 12 346 449
Fragments arranged by size:
1012 502 467 449 346 335 246 244
86 23 12
BstZ17I GTA'TAC
Cuts at: 369 369
Size: 3722
BtgI C'CryG_G
Cuts at: 107 342 350 107
Size: 235 8 3479
Fragments arranged by size:
3479 235 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1672 1853 2016 1672
Size: 181 163 3378
Fragments arranged by size:
3378 181 163
BtsI GCAGTG_nn'
Cuts at: 965 965
Size: 3722
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 1428 2286 1428
Size: 858 2864
EaeI y'GGCC_r
Cuts at: 110 303 1307 1481 1872 1899 3244 110
Size: 193 1004 174 391 27 1345 588
Fragments arranged by size:
1345 1004 588 391 193 174 27
EagI C'GGCC_G
Cuts at: 1307 1307
Size: 3722
EarI CTCTTCn'nnn_
Cuts at: 136 1745 1955 136
Size: 1609 210 1903
Fragments arranged by size:
1903 1609 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1930 2240 2386 3532 1930
Size: 310 146 1146 2120
Fragments arranged by size:
2120 1146 310 146
EcoICRI GAG'CTC
Cuts at: 734 734
Size: 3722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1979 2726 2994 1547
Size: 432 747 268 2275
Fragments arranged by size:
2275 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 861 861
Size: 3722
EcoRI G'AATT_C
Cuts at: 726 726
Size: 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FspI TGC'GCA
Cuts at: 1503 1503
Size: 3722
HaeII r_GCGC'y
Cuts at: 456 1156 1404 2426 3405 456
Size: 700 248 1022 979 773
Fragments arranged by size:
1022 979 773 700 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 1564 1594 1596 1626 373
Size: 32 1159 30 2 30 2469
Fragments arranged by size:
2469 1159 32 30 30 2
HincII GTy'rAC
Cuts at: 761 761
Size: 3722
KasI G'GCGC_C
Cuts at: 1152 1400 1152
Size: 248 3474
KpnI G_GTAC'C
Cuts at: 742 742
Size: 3722
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2577 2393
Size: 184 3538
MscI TGG'CCA
Cuts at: 112 305 1483 112
Size: 193 1178 2351
Fragments arranged by size:
2351 1178 193
MslI CAynn'nnrTG
Cuts at: 1711 1838 1711
Size: 127 3595
MspA1I CmG'CkG
Cuts at: 833 1147 1507 2520 2765 833
Size: 314 360 1013 245 1790
Fragments arranged by size:
1790 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1903 1903
Size: 3722
NarI GG'CG_CC
Cuts at: 1153 1401 1153
Size: 248 3474
NcoI C'CATG_G
Cuts at: 107 350 107
Size: 243 3479
NgoMIV G'CCGG_C
Cuts at: 1901 1901
Size: 3722
NspI r_CATG'y
Cuts at: 775 1275 2182 775
Size: 500 907 2315
Fragments arranged by size:
2315 907 500
PasI CC'CwG_GG
Cuts at: 711 711
Size: 3722
PciI A'CATG_T
Cuts at: 2178 2178
Size: 3722
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PstI C_TGCA'G
Cuts at: 769 1454 769
Size: 685 3037
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 1147 1507 1147
Size: 360 3362
RsrII CG'GwC_CG
Cuts at: 1917 1917
Size: 3722
SacI G_AGCT'C
Cuts at: 736 736
Size: 3722
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
SbfI CC_TGCA'GG
Cuts at: 769 769
Size: 3722
ScaI AGT'ACT
Cuts at: 666 3239 666
Size: 2573 1149
SfcI C'TryA_G
Cuts at: 33 765 1450 2443 2634 33
Size: 732 685 993 191 1121
Fragments arranged by size:
1121 993 732 685 191
SfoI GGC'GCC
Cuts at: 1154 1402 1154
Size: 248 3474
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 607 744 607
Size: 137 3585
SmlI C'TyrA_G
Cuts at: 527 619 877 1792 2284 2546 527
Size: 92 258 915 492 262 1703
Fragments arranged by size:
1703 915 492 262 258 92
SpeI A'CTAG_T
Cuts at: 67 556 67
Size: 489 3233
SphI G_CATG'C
Cuts at: 775 1275 775
Size: 500 3222
SspI AAT'ATT
Cuts at: 402 402
Size: 3722
StyI C'CwwG_G
Cuts at: 107 350 856 1210 107
Size: 243 506 354 2619
Fragments arranged by size:
2619 506 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1305 1438 1897 2106 1305
Size: 133 459 209 2921
Fragments arranged by size:
2921 459 209 133
TatI w'GTAC_w
Cuts at: 664 3237 664
Size: 2573 1149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 459 491 3239 3271 459
Size: 32 2748 32 910
Fragments arranged by size:
2748 910 32 32
Tth111I GACn'n_nGTC
Cuts at: 1519 1519
Size: 3722
XbaI T'CTAG_A
Cuts at: 753 753
Size: 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 576 1217 576
Size: 641 3081
XhoI C'TCGA_G
Cuts at: 527 527
Size: 3722
XmaI C'CCGG_G
Cuts at: 605 742 605
Size: 137 3585
XmnI GAAnn'nnTTC
Cuts at: 391 391
Size: 3722
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsiWI BsmBI BsrGI
BssHII BstEII BstXI Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI