Restriction map for
pF1KB8204
(Circular) (Six-base) MAPSORT of: pF1KB8204.seq Check: 4034 from: 1 to: 4523
pF1KB8204 4523 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1561 1561
Size: 4523
Acc65I G'GTAC_C
Cuts at: 1539 1539
Size: 4523
AclI AA'CG_TT
Cuts at: 4363 4363
Size: 4523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 529 1048 1100 1412 2348 2780 3527 3795
Size: 423 519 52 312 936 432 747 268
Cuts at: 3795 106
Size: 834
Fragments arranged by size:
936 834 747 519 432 423 312 268
52
AflIII A'CryG_T
Cuts at: 1875 2979 3956 1875
Size: 1104 977 2442
Fragments arranged by size:
2442 1104 977
AgeI A'CCGG_T
Cuts at: 2911 2911
Size: 4523
AhdI GACnn_n'nnGTC
Cuts at: 116 116
Size: 4523
AlwNI CAG_nnn'CTG
Cuts at: 927 1488 1500 1828 3395 927
Size: 561 12 328 1567 2055
Fragments arranged by size:
2055 1567 561 328 12
ApaI G_GGCC'C
Cuts at: 202 202
Size: 4523
ApaLI G'TGCA_C
Cuts at: 3293 3293
Size: 4523
ApoI r'AATT_y
Cuts at: 1527 3876 4132 1527
Size: 2349 256 1918
Fragments arranged by size:
2349 1918 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4523
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
AvaI C'yCGr_G
Cuts at: 151 204 295 418 1543 151
Size: 53 91 123 1125 3131
Fragments arranged by size:
3131 1125 123 91 53
BamHI G'GATC_C
Cuts at: 864 1548 864
Size: 684 3839
BanI G'GyrC_C
Cuts at: 189 330 1539 1953 2201 2236 189
Size: 141 1209 414 248 35 2476
Fragments arranged by size:
2476 1209 414 248 141 35
BanII G_rGCy'C
Cuts at: 171 202 211 269 1537 2567 171
Size: 31 9 58 1268 1030 2127
Fragments arranged by size:
2127 1268 1030 58 31 9
BbeI G_GCGC'C
Cuts at: 193 1957 2205 193
Size: 1764 248 2511
Fragments arranged by size:
2511 1764 248
BbsI GAAGACnn'nnnn_
Cuts at: 112 949 1135 112
Size: 837 186 3500
Fragments arranged by size:
3500 837 186
BbvCI CC'TCA_GC
Cuts at: 584 584
Size: 4523
BciVI GTATCCnnnnn_n'
Cuts at: 2421 3182 4523 2421
Size: 761 1341 2421
Fragments arranged by size:
2421 1341 761
BclI T'GATC_A
Cuts at: 2043 2043
Size: 4523
BglI GCCn_nnn'nGGC
Cuts at: 206 206
Size: 4523
BglII A'GATC_T
Cuts at: 711 2038 4075 711
Size: 1327 2037 1159
Fragments arranged by size:
2037 1327 1159
BlpI GC'TnA_GC
Cuts at: 909 1297 1635 909
Size: 388 338 3797
Fragments arranged by size:
3797 388 338
Bme1580I G_kGCm'C
Cuts at: 202 335 2148 2241 3297 202
Size: 133 1813 93 1056 1428
Fragments arranged by size:
1813 1428 1056 133 93
BmgBI CAC'GTC
Cuts at: 1152 1152
Size: 4523
BmrI ACTGGGnnnn_n'
Cuts at: 846 1735 1816 2151 4295 846
Size: 889 81 335 2144 1074
Fragments arranged by size:
2144 1074 889 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 4523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 185 1290 1510 98
Size: 87 1105 220 3111
Fragments arranged by size:
3111 1105 220 87
Bpu10I CC'TnA_GC
Cuts at: 584 584
Size: 4523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 1345 1699 2578 3070 3368 1258
Size: 87 354 879 492 298 2413
Fragments arranged by size:
2413 879 492 354 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 1304 4227 1304
Size: 2923 1600
BsaAI yAC'GTr
Cuts at: 1876 1876
Size: 4523
BsaBI GATnn'nnATC
Cuts at: 6 2037 4074 6
Size: 2031 2037 455
Fragments arranged by size:
2037 2031 455
BsaHI Gr'CG_yC
Cuts at: 190 1954 2202 2904 4400 190
Size: 1764 248 702 1496 313
Fragments arranged by size:
1764 1496 702 313 248
BsaWI w'CCGG_w
Cuts at: 1712 2233 2911 3185 3332 3743 1712
Size: 521 678 274 147 411 2492
Fragments arranged by size:
2492 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 342 372 342
Size: 30 4493
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 381 817 1177 1411 381
Size: 436 360 234 3493
Fragments arranged by size:
3493 436 360 234
BseYI C'CCAG_C
Cuts at: 649 885 1046 1948 2706 3283 3779 649
Size: 236 161 902 758 577 496 1393
Fragments arranged by size:
1393 902 758 577 496 236 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 801 877 801
Size: 76 4447
BsiEI CG_ry'CG
Cuts at: 104 2065 2111 3319 104
Size: 1961 46 1208 1308
Fragments arranged by size:
1961 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1230 1537 2315 2505 3297 1230
Size: 307 778 190 792 2456
Fragments arranged by size:
2456 792 778 307 190
BsmBI CGTCTCn'nnnn_
Cuts at: 763 1243 763
Size: 480 4043
Bsp1286I G_dGCh'C
Cuts at: 171 202 211 269 335 1230 1537 2148 2241
Size: 31 9 58 66 895 307 611 93
Cuts at: 2241 2315 2505 2567 3297 171
Size: 74 190 62 730 1397
Fragments arranged by size:
1397 895 730 611 307 190 93 74
66 62 58 31 9
BspEI T'CCGG_A
Cuts at: 3743 3743
Size: 4523
BspHI T'CATG_A
Cuts at: 3699 3699
Size: 4523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1343 1573 1787 2089 2470 1343
Size: 230 214 302 381 3396
Fragments arranged by size:
3396 381 302 230 214
BsrBI CCG'CTC
Cuts at: 50 908 1343 2815 2869 4353 4518 50
Size: 858 435 1472 54 1484 165 55
Fragments arranged by size:
1484 1472 858 435 165 55 54
BsrDI GCAATG_nn'
Cuts at: 602 2435 602
Size: 1833 2690
BsrFI r'CCGG_y
Cuts at: 327 1381 2521 2702 2911 327
Size: 1054 1140 181 209 1939
Fragments arranged by size:
1939 1140 1054 209 181
BssSI C'ACGA_G
Cuts at: 2794 3152 2794
Size: 358 4165
BstAPI GCAn_nnn'nTGC
Cuts at: 1900 1900
Size: 4523
BstBI TT'CG_AA
Cuts at: 2884 2884
Size: 4523
BstYI r'GATC_y
Cuts at: 1 711 864 1241 1277 1466 1548 2015 2038
Size: 710 153 377 36 189 82 467 23
Cuts at: 2038 2373 2619 3631 3717 3729 4075 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 710 467 449 377 346 335 246
189 153 86 82 36 23 12
BtgI C'CryG_G
Cuts at: 137 194 254 1202 137
Size: 57 60 948 3458
Fragments arranged by size:
3458 948 60 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2473 2654 2817 2473
Size: 181 163 4179
Fragments arranged by size:
4179 181 163
BtsI GCAGTG_nn'
Cuts at: 363 1766 363
Size: 1403 3120
DraI TTT'AAA
Cuts at: 1522 1522
Size: 4523
DrdI GACnn_nn'nnGTC
Cuts at: 1084 1257 2229 3087 1084
Size: 173 972 858 2520
Fragments arranged by size:
2520 972 858 173
EaeI y'GGCC_r
Cuts at: 1205 2108 2282 2673 2700 4045 1205
Size: 903 174 391 27 1345 1683
Fragments arranged by size:
1683 1345 903 391 174 27
EagI C'GGCC_G
Cuts at: 2108 2108
Size: 4523
EarI CTCTTCn'nnn_
Cuts at: 505 678 1388 2546 2756 505
Size: 173 710 1158 210 2272
Fragments arranged by size:
2272 1158 710 210 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2731 3041 3187 4333 2731
Size: 310 146 1146 2921
Fragments arranged by size:
2921 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1535 1535
Size: 4523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 106 185 529 1048 1100 1290 1412 1510
Size: 8 79 344 519 52 190 122 98
Cuts at: 1510 2348 2780 3527 3795 98
Size: 838 432 747 268 826
Fragments arranged by size:
838 826 747 519 432 344 268 190
122 98 79 52 8
EcoO109I rG'GnC_Cy
Cuts at: 199 801 924 1356 1662 199
Size: 602 123 432 306 3060
Fragments arranged by size:
3060 602 432 306 123
EcoRI G'AATT_C
Cuts at: 1527 1527
Size: 4523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4523
FspI TGC'GCA
Cuts at: 1510 2304 1510
Size: 794 3729
HaeII r_GCGC'y
Cuts at: 150 193 289 1053 1404 1957 2205 3227 4206
Size: 43 96 764 351 553 248 1022 979
Cuts at: 4206 150
Size: 467
Fragments arranged by size:
1022 979 764 553 467 351 248 96
43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 533 565 1212 1244 2365 2395 2397
Size: 32 399 32 647 32 1121 30 2
Cuts at: 2397 2427 102
Size: 30 2198
Fragments arranged by size:
2198 1121 647 399 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1562 1562
Size: 4523
HindIII A'AGCT_T
Cuts at: 1299 1299
Size: 4523
KasI G'GCGC_C
Cuts at: 189 1953 2201 189
Size: 1764 248 2511
Fragments arranged by size:
2511 1764 248
KpnI G_GTAC'C
Cuts at: 1543 1543
Size: 4523
MfeI C'AATT_G
Cuts at: 1082 1082
Size: 4523
MluI A'CGCG_T
Cuts at: 3956 3956
Size: 4523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 578 617 3194 3378 578
Size: 39 2577 184 1723
Fragments arranged by size:
2577 1723 184 39
MscI TGG'CCA
Cuts at: 2284 2284
Size: 4523
MslI CAynn'nnrTG
Cuts at: 1105 1150 2512 2639 1105
Size: 45 1362 127 2989
Fragments arranged by size:
2989 1362 127 45
MspA1I CmG'CkG
Cuts at: 256 1634 1948 2308 3321 3566 256
Size: 1378 314 360 1013 245 1213
Fragments arranged by size:
1378 1213 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1383 2704 1383
Size: 1321 3202
NarI GG'CG_CC
Cuts at: 190 1954 2202 190
Size: 1764 248 2511
Fragments arranged by size:
2511 1764 248
NcoI C'CATG_G
Cuts at: 194 194
Size: 4523
NgoMIV G'CCGG_C
Cuts at: 1381 2702 1381
Size: 1321 3202
NspI r_CATG'y
Cuts at: 1576 2076 2983 1576
Size: 500 907 3116
Fragments arranged by size:
3116 907 500
PciI A'CATG_T
Cuts at: 2979 2979
Size: 4523
PfoI T'CCnGG_A
Cuts at: 497 638 497
Size: 141 4382
PmeI GTTT'AAAC
Cuts at: 1522 1522
Size: 4523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4491
PpuMI rG'GwC_Cy
Cuts at: 801 1356 801
Size: 555 3968
PspOMI G'GGCC_C
Cuts at: 198 198
Size: 4523
PstI C_TGCA'G
Cuts at: 959 1570 2255 959
Size: 611 685 3227
Fragments arranged by size:
3227 685 611
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4523
PvuII CAG'CTG
Cuts at: 1948 2308 1948
Size: 360 4163
RsrII CG'GwC_CG
Cuts at: 2718 2718
Size: 4523
SacI G_AGCT'C
Cuts at: 1537 1537
Size: 4523
SacII CC_GC'GG
Cuts at: 257 257
Size: 4523
SalI G'TCGA_C
Cuts at: 1560 1560
Size: 4523
SapI GCTCTTCn'nnn_
Cuts at: 2546 2756 2546
Size: 210 4313
SbfI CC_TGCA'GG
Cuts at: 1570 1570
Size: 4523
ScaI AGT'ACT
Cuts at: 4040 4040
Size: 4523
SfcI C'TryA_G
Cuts at: 33 955 1566 2251 3244 3435 33
Size: 922 611 685 993 191 1121
Fragments arranged by size:
1121 993 922 685 611 191
SfoI GGC'GCC
Cuts at: 191 1955 2203 191
Size: 1764 248 2511
Fragments arranged by size:
2511 1764 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
SmaI CCC'GGG
Cuts at: 153 206 297 1545 153
Size: 53 91 1248 3131
Fragments arranged by size:
3131 1248 91 53
SmlI C'TyrA_G
Cuts at: 1273 1360 1678 2593 3085 3347 1273
Size: 87 318 915 492 262 2449
Fragments arranged by size:
2449 915 492 318 262 87
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4523
SphI G_CATG'C
Cuts at: 1576 2076 1576
Size: 500 4023
SrfI GCCC'GGGC
Cuts at: 297 297
Size: 4523
StyI C'CwwG_G
Cuts at: 194 1657 2011 194
Size: 1463 354 2706
Fragments arranged by size:
2706 1463 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 143 147 771 2106 2239 2698 2907 143
Size: 4 624 1335 133 459 209 1759
Fragments arranged by size:
1759 1335 624 459 209 133 4
TatI w'GTAC_w
Cuts at: 4038 4038
Size: 4523
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 342 374 4040 4072 342
Size: 32 3666 32 793
Fragments arranged by size:
3666 793 32 32
Tth111I GACn'n_nGTC
Cuts at: 2320 2320
Size: 4523
XbaI T'CTAG_A
Cuts at: 1554 1554
Size: 4523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2018 2018
Size: 4523
XmaI C'CCGG_G
Cuts at: 151 204 295 1543 151
Size: 53 91 1248 3131
Fragments arranged by size:
3131 1248 91 53
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI