Restriction map for
pF1KB8206
(Circular) (Six-base) MAPSORT of: pF1KB8206.seq Check: 5406 from: 1 to: 3920
pF1KB8206 3920 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1745 2177 2924 3192 1745
Size: 432 747 268 2473
Fragments arranged by size:
2473 747 432 268
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 436 2308 436
Size: 1872 2048
AleI CACnn'nnGTG
Cuts at: 647 647
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 862 1225 2792 862
Size: 363 1567 1990
Fragments arranged by size:
1990 1567 363
ApaLI G'TGCA_C
Cuts at: 845 2690 845
Size: 1845 2075
ApoI r'AATT_y
Cuts at: 924 3273 3529 924
Size: 2349 256 1315
Fragments arranged by size:
2349 1315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 113 676 940 113
Size: 563 264 3093
Fragments arranged by size:
3093 563 264
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 162 936 1350 1598 1633 162
Size: 774 414 248 35 2449
Fragments arranged by size:
2449 774 414 248 35
BanII G_rGCy'C
Cuts at: 275 677 755 934 1964 275
Size: 402 78 179 1030 2231
Fragments arranged by size:
2231 1030 402 179 78
BbeI G_GCGC'C
Cuts at: 166 1354 1602 166
Size: 1188 248 2484
Fragments arranged by size:
2484 1188 248
BbsI GAAGACnn'nnnn_
Cuts at: 884 884
Size: 3920
BbvCI CC'TCA_GC
Cuts at: 463 463
Size: 3920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 170 204 170
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglI GCCn_nnn'nGGC
Cuts at: 151 151
Size: 3920
BglII A'GATC_T
Cuts at: 822 872 1435 3472 822
Size: 50 563 2037 1270
Fragments arranged by size:
2037 1270 563 50
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 156 193 654 849 1545 1638 2694 156
Size: 37 461 195 696 93 1056 1382
Fragments arranged by size:
1382 1056 696 461 195 93 37
BmrI ACTGGGnnnn_n'
Cuts at: 907 1132 1213 1548 3692 907
Size: 225 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 367 852 2287 367
Size: 485 1435 2000
Fragments arranged by size:
2000 1435 485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 847
Size: 3920
Bpu10I CC'TnA_GC
Cuts at: 463 463
Size: 3920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1975 2467 2765 1096
Size: 879 492 298 2251
Fragments arranged by size:
2251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 1434 3471 6
Size: 1428 2037 455
Fragments arranged by size:
2037 1428 455
BsaHI Gr'CG_yC
Cuts at: 163 1351 1599 2301 3797 163
Size: 1188 248 702 1496 286
Fragments arranged by size:
1496 1188 702 286 248
BsaWI w'CCGG_w
Cuts at: 436 742 772 1109 1630 2308 2582 2729 3140
Size: 306 30 337 521 678 274 147 411
Cuts at: 3140 436
Size: 1216
Fragments arranged by size:
1216 678 521 411 337 306 274 147
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 576 154
Size: 422 3498
BseYI C'CCAG_C
Cuts at: 480 1345 2103 2680 3176 480
Size: 865 758 577 496 1224
Fragments arranged by size:
1224 865 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 240
Size: 3920
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 275 755 849 934 1712 1902 2694 275
Size: 480 94 85 778 190 792 1501
Fragments arranged by size:
1501 792 778 480 190 94 85
Bsp1286I G_dGCh'C
Cuts at: 156 193 275 654 677 755 849 934 1545
Size: 37 82 379 23 78 94 85 611
Cuts at: 1545 1638 1712 1902 1964 2694 156
Size: 93 74 190 62 730 1382
Fragments arranged by size:
1382 730 611 379 190 94 93 85
82 78 74 62 37 23
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 583 970 1184 1486 1867 583
Size: 387 214 302 381 2636
Fragments arranged by size:
2636 387 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 436 1918 2099 2308 436
Size: 1482 181 209 2048
Fragments arranged by size:
2048 1482 209 181
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstEII G'GTnAC_C
Cuts at: 687 687
Size: 3920
BstXI CCAn_nnnn'nTGG
Cuts at: 421 421
Size: 3920
BstYI r'GATC_y
Cuts at: 1 822 872 945 1412 1435 1770 2016 3028
Size: 821 50 73 467 23 335 246 1012
Cuts at: 3028 3114 3126 3472 1
Size: 86 12 346 449
Fragments arranged by size:
1012 821 467 449 346 335 246 86
73 50 23 12
BtgI C'CryG_G
Cuts at: 107 414 881 107
Size: 307 467 3146
Fragments arranged by size:
3146 467 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 231 442 1163 231
Size: 211 721 2988
Fragments arranged by size:
2988 721 211
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 1626 2484 1626
Size: 858 3062
EaeI y'GGCC_r
Cuts at: 1505 1679 2070 2097 3442 1505
Size: 174 391 27 1345 1983
Fragments arranged by size:
1983 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 322 2128 2438 2584 3730 322
Size: 1806 310 146 1146 512
Fragments arranged by size:
1806 1146 512 310 146
EcoICRI GAG'CTC
Cuts at: 273 753 932 273
Size: 480 179 3261
Fragments arranged by size:
3261 480 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1745 2177 2924 3192 847
Size: 898 432 747 268 1575
Fragments arranged by size:
1575 898 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1059 1059
Size: 3920
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3920
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 166 1354 1602 2624 3603 166
Size: 1188 248 1022 979 483
Fragments arranged by size:
1188 1022 979 483 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1762 1792 1794 1824 1762
Size: 30 2 30 3858
Fragments arranged by size:
3858 30 30 2
HincII GTy'rAC
Cuts at: 959 959
Size: 3920
KasI G'GCGC_C
Cuts at: 162 1350 1598 162
Size: 1188 248 2484
Fragments arranged by size:
2484 1188 248
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 2775 2591
Size: 184 3736
MscI TGG'CCA
Cuts at: 1681 1681
Size: 3920
MslI CAynn'nnrTG
Cuts at: 647 1909 2036 647
Size: 1262 127 2531
Fragments arranged by size:
2531 1262 127
MspA1I CmG'CkG
Cuts at: 125 372 596 1031 1345 1705 2718 2963 125
Size: 247 224 435 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 435 360 314 247 245 224
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 163 1351 1599 163
Size: 1188 248 2484
Fragments arranged by size:
2484 1188 248
NcoI C'CATG_G
Cuts at: 107 414 881 107
Size: 307 467 3146
Fragments arranged by size:
3146 467 307
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NspI r_CATG'y
Cuts at: 413 973 1473 2380 413
Size: 560 500 907 1953
Fragments arranged by size:
1953 907 560 500
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PstI C_TGCA'G
Cuts at: 967 1652 967
Size: 685 3235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 596 1345 1705 596
Size: 749 360 2811
Fragments arranged by size:
2811 749 360
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 275 755 934 275
Size: 480 179 3261
Fragments arranged by size:
3261 480 179
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 963 1648 2641 2832 33
Size: 930 685 993 191 1121
Fragments arranged by size:
1121 993 930 685 191
SfoI GGC'GCC
Cuts at: 164 1352 1600 164
Size: 1188 248 2484
Fragments arranged by size:
2484 1188 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 678 942 678
Size: 264 3656
SmlI C'TyrA_G
Cuts at: 1075 1990 2482 2744 1075
Size: 915 492 262 2251
Fragments arranged by size:
2251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 413 973 1473 413
Size: 560 500 2860
Fragments arranged by size:
2860 560 500
StuI AGG'CCT
Cuts at: 145 145
Size: 3920
StyI C'CwwG_G
Cuts at: 107 414 881 1054 1408 107
Size: 307 467 173 354 2619
Fragments arranged by size:
2619 467 354 307 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 3435 3435
Size: 3920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 608 640 3437 3469 608
Size: 32 2797 32 1059
Fragments arranged by size:
2797 1059 32 32
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 676 940 676
Size: 264 3656
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI