Restriction map for
pF1KB8211
(Circular) (Six-base) MAPSORT of: pF1KB8211.seq Check: 1361 from: 1 to: 4202
pF1KB8211 4202 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1240 1240
Size: 4202
Acc65I G'GTAC_C
Cuts at: 1218 1218
Size: 4202
AclI AA'CG_TT
Cuts at: 4042 4042
Size: 4202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2459 3206 3474 2027
Size: 432 747 268 2755
Fragments arranged by size:
2755 747 432 268
AflIII A'CryG_T
Cuts at: 905 1554 2658 3635 905
Size: 649 1104 977 1472
Fragments arranged by size:
1472 1104 977 649
AgeI A'CCGG_T
Cuts at: 2590 2590
Size: 4202
AleI CACnn'nnGTG
Cuts at: 203 203
Size: 4202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 221 221
Size: 4202
AlwNI CAG_nnn'CTG
Cuts at: 1507 3074 1507
Size: 1567 2635
ApaI G_GGCC'C
Cuts at: 508 674 751 851 982 508
Size: 166 77 100 131 3728
Fragments arranged by size:
3728 166 131 100 77
ApaLI G'TGCA_C
Cuts at: 2972 2972
Size: 4202
ApoI r'AATT_y
Cuts at: 1206 3555 3811 1206
Size: 2349 256 1597
Fragments arranged by size:
2349 1597 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4202
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
AvaI C'yCGr_G
Cuts at: 1035 1098 1158 1222 1035
Size: 63 60 64 4015
Fragments arranged by size:
4015 64 63 60
BamHI G'GATC_C
Cuts at: 1024 1227 1024
Size: 203 3999
BanI G'GyrC_C
Cuts at: 205 327 577 937 1072 1102 1218 1632 1880
Size: 122 250 360 135 30 116 414 248
Cuts at: 1880 1915 205
Size: 35 2492
Fragments arranged by size:
2492 414 360 250 248 135 122 116
35 30
BanII G_rGCy'C
Cuts at: 275 508 590 603 674 751 851 982 1036
Size: 233 82 13 71 77 100 131 54
Cuts at: 1036 1216 2246 275
Size: 180 1030 2231
Fragments arranged by size:
2231 1030 233 180 131 100 82 77
71 54 13
BbeI G_GCGC'C
Cuts at: 331 581 941 1076 1636 1884 331
Size: 250 360 135 560 248 2649
Fragments arranged by size:
2649 560 360 250 248 135
BbsI GAAGACnn'nnnn_
Cuts at: 297 297
Size: 4202
BciVI GTATCCnnnnn_n'
Cuts at: 2100 2861 4202 2100
Size: 761 1341 2100
Fragments arranged by size:
2100 1341 761
BclI T'GATC_A
Cuts at: 1722 1722
Size: 4202
BglI GCCn_nnn'nGGC
Cuts at: 554 946 977 554
Size: 392 31 3779
Fragments arranged by size:
3779 392 31
BglII A'GATC_T
Cuts at: 1717 3754 1717
Size: 2037 2165
BlpI GC'TnA_GC
Cuts at: 1314 1314
Size: 4202
Bme1580I G_kGCm'C
Cuts at: 143 210 508 674 751 851 982 1105 1827
Size: 67 298 166 77 100 131 123 722
Cuts at: 1827 1920 2976 143
Size: 93 1056 1369
Fragments arranged by size:
1369 1056 722 298 166 131 123 100
93 77 67
BmrI ACTGGGnnnn_n'
Cuts at: 773 1414 1495 1830 3974 773
Size: 641 81 335 2144 1001
Fragments arranged by size:
2144 1001 641 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 554 2569 554
Size: 2015 2187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 899 879
Size: 20 4182
Bpu10I CC'TnA_GC
Cuts at: 1087 1087
Size: 4202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1378 2257 2749 3047 1378
Size: 879 492 298 2533
Fragments arranged by size:
2533 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3906 3906
Size: 4202
BsaAI yAC'GTr
Cuts at: 1017 1555 1017
Size: 538 3664
BsaBI GATnn'nnATC
Cuts at: 6 1716 3753 6
Size: 1710 2037 455
Fragments arranged by size:
2037 1710 455
BsaHI Gr'CG_yC
Cuts at: 328 578 643 938 1073 1633 1881 2583 4079
Size: 250 65 295 135 560 248 702 1496
Cuts at: 4079 328
Size: 451
Fragments arranged by size:
1496 702 560 451 295 250 248 135
65
BsaWI w'CCGG_w
Cuts at: 826 1391 1912 2590 2864 3011 3422 826
Size: 565 521 678 274 147 411 1606
Fragments arranged by size:
1606 678 565 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 638 792 795 854 559
Size: 79 154 3 59 3907
Fragments arranged by size:
3907 154 79 59 3
BseYI C'CCAG_C
Cuts at: 264 403 560 1627 2385 2962 3458 264
Size: 139 157 1067 758 577 496 1008
Fragments arranged by size:
1067 1008 758 577 496 157 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 271 1132 181
Size: 90 861 3251
Fragments arranged by size:
3251 861 90
BsiEI CG_ry'CG
Cuts at: 104 137 1133 1744 1790 2998 104
Size: 33 996 611 46 1208 1308
Fragments arranged by size:
1308 1208 996 611 46 33
BsiHKAI G_wGCw'C
Cuts at: 356 1216 1994 2184 2976 356
Size: 860 778 190 792 1582
Fragments arranged by size:
1582 860 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 143 210 275 356 508 590 603 674 751
Size: 67 65 81 152 82 13 71 77
Cuts at: 751 851 982 1036 1105 1216 1827 1920 1994
Size: 100 131 54 69 111 611 93 74
Cuts at: 1994 2184 2246 2976 143
Size: 190 62 730 1369
Fragments arranged by size:
1369 730 611 190 152 131 111 100
93 82 81 77 74 71 69 67
65 62 54 13
BspEI T'CCGG_A
Cuts at: 3422 3422
Size: 4202
BspHI T'CATG_A
Cuts at: 917 3378 917
Size: 2461 1741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 200 1252 1466 1768 2149 200
Size: 1052 214 302 381 2253
Fragments arranged by size:
2253 1052 381 302 214
BsrBI CCG'CTC
Cuts at: 50 960 2494 2548 4032 4197 50
Size: 910 1534 54 1484 165 55
Fragments arranged by size:
1534 1484 910 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2114 2114
Size: 4202
BsrFI r'CCGG_y
Cuts at: 718 940 1081 2200 2381 2590 718
Size: 222 141 1119 181 209 2330
Fragments arranged by size:
2330 1119 222 209 181 141
BsrGI T'GTAC_A
Cuts at: 908 908
Size: 4202
BssSI C'ACGA_G
Cuts at: 596 650 2473 2831 596
Size: 54 1823 358 1967
Fragments arranged by size:
1967 1823 358 54
BstAPI GCAn_nnn'nTGC
Cuts at: 1579 1579
Size: 4202
BstBI TT'CG_AA
Cuts at: 2563 2563
Size: 4202
BstXI CCAn_nnnn'nTGG
Cuts at: 682 744 1194 682
Size: 62 450 3690
Fragments arranged by size:
3690 450 62
BstYI r'GATC_y
Cuts at: 1 239 1024 1227 1694 1717 2052 2298 3310
Size: 238 785 203 467 23 335 246 1012
Cuts at: 3310 3396 3408 3754 1
Size: 86 12 346 449
Fragments arranged by size:
1012 785 467 449 346 335 246 238
203 86 23 12
BtgI C'CryG_G
Cuts at: 455 581 766 983 1076 1108 455
Size: 126 185 217 93 32 3549
Fragments arranged by size:
3549 217 185 126 93 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1015 2152 2333 2496 1015
Size: 1137 181 163 2721
Fragments arranged by size:
2721 1137 181 163
BtsI GCAGTG_nn'
Cuts at: 1445 1445
Size: 4202
DraI TTT'AAA
Cuts at: 1201 1201
Size: 4202
DraIII CAC_nnn'GTG
Cuts at: 1017 1017
Size: 4202
DrdI GACnn_nn'nnGTC
Cuts at: 231 1908 2766 231
Size: 1677 858 1667
Fragments arranged by size:
1677 1667 858
EaeI y'GGCC_r
Cuts at: 134 443 659 986 1079 1130 1787 1961 2352
Size: 309 216 327 93 51 657 174 391
Cuts at: 2352 2379 3724 134
Size: 27 1345 612
Fragments arranged by size:
1345 657 612 391 327 309 216 174
93 51 27
EagI C'GGCC_G
Cuts at: 134 1130 1787 134
Size: 996 657 2549
Fragments arranged by size:
2549 996 657
EarI CTCTTCn'nnn_
Cuts at: 333 1054 2225 2435 333
Size: 721 1171 210 2100
Fragments arranged by size:
2100 1171 721 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2410 2720 2866 4012 2410
Size: 310 146 1146 2600
Fragments arranged by size:
2600 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1214 1214
Size: 4202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 899 2027 2459 3206 3474 879
Size: 20 1128 432 747 268 1607
Fragments arranged by size:
1607 1128 747 432 268 20
EcoNI CCTnn'n_nnAGG
Cuts at: 542 542
Size: 4202
EcoO109I rG'GnC_Cy
Cuts at: 312 504 505 670 671 848 978 979 1139
Size: 192 1 165 1 177 130 1 160
Cuts at: 1139 1341 312
Size: 202 3173
Fragments arranged by size:
3173 202 192 177 165 160 130 1
1 1
EcoRI G'AATT_C
Cuts at: 1206 1206
Size: 4202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 4202
FseI GG_CCGG'CC
Cuts at: 1085 1085
Size: 4202
FspI TGC'GCA
Cuts at: 1983 1983
Size: 4202
HaeII r_GCGC'y
Cuts at: 175 331 581 941 1076 1636 1884 2906 3885
Size: 156 250 360 135 560 248 1022 979
Cuts at: 3885 175
Size: 492
Fragments arranged by size:
1022 979 560 492 360 250 248 156
135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 516 548 633 665 993 1025 2044 2074 2076
Size: 32 85 32 328 32 1019 30 2
Cuts at: 2076 2106 516
Size: 30 2612
Fragments arranged by size:
2612 1019 328 85 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1241 1241
Size: 4202
KasI G'GCGC_C
Cuts at: 327 577 937 1072 1632 1880 327
Size: 250 360 135 560 248 2649
Fragments arranged by size:
2649 560 360 250 248 135
KpnI G_GTAC'C
Cuts at: 1222 1222
Size: 4202
MluI A'CGCG_T
Cuts at: 3635 3635
Size: 4202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 170 2873 3057 170
Size: 2703 184 1315
Fragments arranged by size:
2703 1315 184
MscI TGG'CCA
Cuts at: 661 1963 661
Size: 1302 2900
MslI CAynn'nnrTG
Cuts at: 203 742 916 1192 2191 2318 203
Size: 539 174 276 999 127 2087
Fragments arranged by size:
2087 999 539 276 174 127
MspA1I CmG'CkG
Cuts at: 1110 1313 1627 1987 3000 3245 1110
Size: 203 314 360 1013 245 2067
Fragments arranged by size:
2067 1013 360 314 245 203
NaeI GCC'GGC
Cuts at: 720 942 1083 2383 720
Size: 222 141 1300 2539
Fragments arranged by size:
2539 1300 222 141
NarI GG'CG_CC
Cuts at: 328 578 938 1073 1633 1881 328
Size: 250 360 135 560 248 2649
Fragments arranged by size:
2649 560 360 250 248 135
NcoI C'CATG_G
Cuts at: 455 581 983 455
Size: 126 402 3674
Fragments arranged by size:
3674 402 126
NgoMIV G'CCGG_C
Cuts at: 718 940 1081 2381 718
Size: 222 141 1300 2539
Fragments arranged by size:
2539 1300 222 141
NotI GC'GGCC_GC
Cuts at: 1130 1130
Size: 4202
NspI r_CATG'y
Cuts at: 696 909 1255 1755 2662 696
Size: 213 346 500 907 2236
Fragments arranged by size:
2236 907 500 346 213
PasI CC'CwG_GG
Cuts at: 277 499 603 1029 277
Size: 222 104 426 3450
Fragments arranged by size:
3450 426 222 104
PciI A'CATG_T
Cuts at: 905 2658 905
Size: 1753 2449
PfoI T'CCnGG_A
Cuts at: 377 377
Size: 4202
PmeI GTTT'AAAC
Cuts at: 1201 1201
Size: 4202
PmlI CAC'GTG
Cuts at: 1017 1017
Size: 4202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 4170
PpuMI rG'GwC_Cy
Cuts at: 312 1139 312
Size: 827 3375
PspOMI G'GGCC_C
Cuts at: 504 670 747 847 978 504
Size: 166 77 100 131 3728
Fragments arranged by size:
3728 166 131 100 77
PstI C_TGCA'G
Cuts at: 396 1249 1934 396
Size: 853 685 2664
Fragments arranged by size:
2664 853 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4202
PvuII CAG'CTG
Cuts at: 1627 1987 1627
Size: 360 3842
RsrII CG'GwC_CG
Cuts at: 2397 2397
Size: 4202
SacI G_AGCT'C
Cuts at: 1216 1216
Size: 4202
SacII CC_GC'GG
Cuts at: 1111 1111
Size: 4202
SalI G'TCGA_C
Cuts at: 1239 1239
Size: 4202
SapI GCTCTTCn'nnn_
Cuts at: 2225 2435 2225
Size: 210 3992
SbfI CC_TGCA'GG
Cuts at: 1249 1249
Size: 4202
ScaI AGT'ACT
Cuts at: 3719 3719
Size: 4202
SfcI C'TryA_G
Cuts at: 33 384 392 1245 1930 2923 3114 33
Size: 351 8 853 685 993 191 1121
Fragments arranged by size:
1121 993 853 685 351 191 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 977 977
Size: 4202
SfoI GGC'GCC
Cuts at: 329 579 939 1074 1634 1882 329
Size: 250 360 135 560 248 2649
Fragments arranged by size:
2649 560 360 250 248 135
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
SmaI CCC'GGG
Cuts at: 1100 1224 1100
Size: 124 4078
SmlI C'TyrA_G
Cuts at: 1357 2272 2764 3026 1357
Size: 915 492 262 2533
Fragments arranged by size:
2533 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4202
SphI G_CATG'C
Cuts at: 696 1255 1755 696
Size: 559 500 3143
Fragments arranged by size:
3143 559 500
StuI AGG'CCT
Cuts at: 346 373 548 728 346
Size: 27 175 180 3820
Fragments arranged by size:
3820 180 175 27
StyI C'CwwG_G
Cuts at: 116 368 455 581 983 1336 1690 116
Size: 252 87 126 402 353 354 2628
Fragments arranged by size:
2628 402 354 353 252 126 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 720 876 1785 1918 2377 2586 720
Size: 156 909 133 459 209 2336
Fragments arranged by size:
2336 909 459 209 156 133
TatI w'GTAC_w
Cuts at: 317 908 3717 317
Size: 591 2809 802
Fragments arranged by size:
2809 802 591
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 479 511 3719 3751 479
Size: 32 3208 32 930
Fragments arranged by size:
3208 930 32 32
Tth111I GACn'n_nGTC
Cuts at: 1999 1999
Size: 4202
XbaI T'CTAG_A
Cuts at: 1233 1233
Size: 4202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 411 999 1697 411
Size: 588 698 2916
Fragments arranged by size:
2916 698 588
XmaI C'CCGG_G
Cuts at: 1098 1222 1098
Size: 124 4078
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PflMI PshAI PsiI PspXI PsrI SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI