Restriction map for
pF1KB8214
(Circular) (Six-base) MAPSORT of: pF1KB8214.seq Check: 6980 from: 1 to: 3884
pF1KB8214 3884 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 702 702
Size: 3884
AccI GT'mk_AC
Cuts at: 922 922
Size: 3884
Acc65I G'GTAC_C
Cuts at: 900 900
Size: 3884
AclI AA'CG_TT
Cuts at: 3724 3724
Size: 3884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1709 2141 2888 3156 1709
Size: 432 747 268 2437
Fragments arranged by size:
2437 747 432 268
AflIII A'CryG_T
Cuts at: 1236 2340 3317 1236
Size: 1104 977 1803
Fragments arranged by size:
1803 1104 977
AgeI A'CCGG_T
Cuts at: 818 2272 818
Size: 1454 2430
AlwNI CAG_nnn'CTG
Cuts at: 621 762 1189 2756 621
Size: 141 427 1567 1749
Fragments arranged by size:
1749 1567 427 141
ApaI G_GGCC'C
Cuts at: 353 353
Size: 3884
ApaLI G'TGCA_C
Cuts at: 2654 2654
Size: 3884
ApoI r'AATT_y
Cuts at: 888 3237 3493 888
Size: 2349 256 1279
Fragments arranged by size:
2349 1279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3884
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
AvaI C'yCGr_G
Cuts at: 352 367 904 352
Size: 15 537 3332
Fragments arranged by size:
3332 537 15
BamHI G'GATC_C
Cuts at: 590 909 590
Size: 319 3565
BanI G'GyrC_C
Cuts at: 169 418 454 664 900 1314 1562 1597 169
Size: 249 36 210 236 414 248 35 2456
Fragments arranged by size:
2456 414 249 248 236 210 36 35
BanII G_rGCy'C
Cuts at: 205 353 359 805 898 1928 205
Size: 148 6 446 93 1030 2161
Fragments arranged by size:
2161 1030 446 148 93 6
BbeI G_GCGC'C
Cuts at: 422 458 668 1318 1566 422
Size: 36 210 650 248 2740
Fragments arranged by size:
2740 650 248 210 36
BciVI GTATCCnnnnn_n'
Cuts at: 1782 2543 3884 1782
Size: 761 1341 1782
Fragments arranged by size:
1782 1341 761
BclI T'GATC_A
Cuts at: 1404 1404
Size: 3884
BglI GCCn_nnn'nGGC
Cuts at: 286 286
Size: 3884
BglII A'GATC_T
Cuts at: 1399 3436 1399
Size: 2037 1847
BlpI GC'TnA_GC
Cuts at: 996 996
Size: 3884
Bme1580I G_kGCm'C
Cuts at: 184 353 542 1509 1602 2658 184
Size: 169 189 967 93 1056 1410
Fragments arranged by size:
1410 1056 967 189 169 93
BmgBI CAC'GTC
Cuts at: 537 537
Size: 3884
BmrI ACTGGGnnnn_n'
Cuts at: 1096 1177 1512 3656 1096
Size: 81 335 2144 1324
Fragments arranged by size:
2144 1324 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 3884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 784 649
Size: 135 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1939 2431 2729 1060
Size: 879 492 298 2215
Fragments arranged by size:
2215 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3588 3588
Size: 3884
BsaAI yAC'GTr
Cuts at: 657 1237 657
Size: 580 3304
BsaBI GATnn'nnATC
Cuts at: 6 1398 3435 6
Size: 1392 2037 455
Fragments arranged by size:
2037 1392 455
BsaHI Gr'CG_yC
Cuts at: 419 455 665 1315 1563 2265 3761 419
Size: 36 210 650 248 702 1496 542
Fragments arranged by size:
1496 702 650 542 248 210 36
BsaWI w'CCGG_w
Cuts at: 734 818 1073 1594 2272 2546 2693 3104 734
Size: 84 255 521 678 274 147 411 1514
Fragments arranged by size:
1514 678 521 411 274 255 147 84
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 781 781
Size: 3884
BseYI C'CCAG_C
Cuts at: 271 414 458 1309 2067 2644 3140 271
Size: 143 44 851 758 577 496 1015
Fragments arranged by size:
1015 851 758 577 496 143 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 544
Size: 3884
BsiEI CG_ry'CG
Cuts at: 104 224 281 317 1426 1472 2680 104
Size: 120 57 36 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 120 57 46 36
BsiHKAI G_wGCw'C
Cuts at: 359 898 1676 1866 2658 359
Size: 539 778 190 792 1585
Fragments arranged by size:
1585 792 778 539 190
BsmBI CGTCTCn'nnnn_
Cuts at: 700 700
Size: 3884
Bsp1286I G_dGCh'C
Cuts at: 184 205 353 359 542 805 898 1509 1602
Size: 21 148 6 183 263 93 611 93
Cuts at: 1602 1676 1866 1928 2658 184
Size: 74 190 62 730 1410
Fragments arranged by size:
1410 730 611 263 190 183 148 93
93 74 62 21 6
BspEI T'CCGG_A
Cuts at: 3104 3104
Size: 3884
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 702 934 1148 1450 1831 702
Size: 232 214 302 381 2755
Fragments arranged by size:
2755 381 302 232 214
BsrBI CCG'CTC
Cuts at: 50 240 335 672 2176 2230 3714 3879 50
Size: 190 95 337 1504 54 1484 165 55
Fragments arranged by size:
1504 1484 337 190 165 95 55 54
BsrDI GCAATG_nn'
Cuts at: 827 1796 827
Size: 969 2915
BsrFI r'CCGG_y
Cuts at: 172 685 818 1882 2063 2272 172
Size: 513 133 1064 181 209 1784
Fragments arranged by size:
1784 1064 513 209 181 133
BssHII G'CGCG_C
Cuts at: 633 633
Size: 3884
BssSI C'ACGA_G
Cuts at: 358 2155 2513 358
Size: 1797 358 1729
Fragments arranged by size:
1797 1729 358
BstAPI GCAn_nnn'nTGC
Cuts at: 621 1261 621
Size: 640 3244
BstBI TT'CG_AA
Cuts at: 2245 2245
Size: 3884
BstYI r'GATC_y
Cuts at: 1 590 909 1376 1399 1734 1980 2992 3078
Size: 589 319 467 23 335 246 1012 86
Cuts at: 3078 3090 3436 1
Size: 12 346 449
Fragments arranged by size:
1012 589 467 449 346 335 319 246
86 23 12
BtgI C'CryG_G
Cuts at: 119 248 311 320 119
Size: 129 63 9 3683
Fragments arranged by size:
3683 129 63 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1834 2015 2178 1834
Size: 181 163 3540
Fragments arranged by size:
3540 181 163
BtsI GCAGTG_nn'
Cuts at: 1127 1127
Size: 3884
ClaI AT'CG_AT
Cuts at: 860 860
Size: 3884
DraI TTT'AAA
Cuts at: 883 883
Size: 3884
DraIII CAC_nnn'GTG
Cuts at: 657 657
Size: 3884
DrdI GACnn_nn'nnGTC
Cuts at: 862 1590 2448 862
Size: 728 858 2298
Fragments arranged by size:
2298 858 728
EaeI y'GGCC_r
Cuts at: 122 155 221 278 314 513 683 1469 1643
Size: 33 66 57 36 199 170 786 174
Cuts at: 1643 2034 2061 3406 122
Size: 391 27 1345 600
Fragments arranged by size:
1345 786 600 391 199 174 170 66
57 36 33 27
EagI C'GGCC_G
Cuts at: 221 278 314 1469 221
Size: 57 36 1155 2636
Fragments arranged by size:
2636 1155 57 36
EarI CTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2092 2402 2548 3694 2092
Size: 310 146 1146 2282
Fragments arranged by size:
2282 1146 310 146
EcoICRI GAG'CTC
Cuts at: 357 896 357
Size: 539 3345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 784 1709 2141 2888 3156 649
Size: 135 925 432 747 268 1377
Fragments arranged by size:
1377 925 747 432 268 135
EcoO109I rG'GnC_Cy
Cuts at: 468 492 775 1023 468
Size: 24 283 248 3329
Fragments arranged by size:
3329 283 248 24
EcoRI G'AATT_C
Cuts at: 888 888
Size: 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 150 1903 150
Size: 1753 2131
FspI TGC'GCA
Cuts at: 1665 1665
Size: 3884
HaeII r_GCGC'y
Cuts at: 256 422 458 668 1318 1566 2588 3567 256
Size: 166 36 210 650 248 1022 979 573
Fragments arranged by size:
1022 979 650 573 248 210 166 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 384 416 853 885 1726 1756 1758 1788 384
Size: 32 437 32 841 30 2 30 2480
Fragments arranged by size:
2480 841 437 32 32 30 30 2
HincII GTy'rAC
Cuts at: 923 923
Size: 3884
KasI G'GCGC_C
Cuts at: 418 454 664 1314 1562 418
Size: 36 210 650 248 2740
Fragments arranged by size:
2740 650 248 210 36
KpnI G_GTAC'C
Cuts at: 904 904
Size: 3884
MluI A'CGCG_T
Cuts at: 3317 3317
Size: 3884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2555 2739 2555
Size: 184 3700
MscI TGG'CCA
Cuts at: 157 1645 157
Size: 1488 2396
MslI CAynn'nnrTG
Cuts at: 1873 2000 1873
Size: 127 3757
MspA1I CmG'CkG
Cuts at: 121 313 322 414 429 618 681 995 1309
Size: 192 9 92 15 189 63 314 314
Cuts at: 1309 1669 2682 2927 121
Size: 360 1013 245 1078
Fragments arranged by size:
1078 1013 360 314 314 245 192 189
92 63 15 9
NaeI GCC'GGC
Cuts at: 687 2065 687
Size: 1378 2506
NarI GG'CG_CC
Cuts at: 419 455 665 1315 1563 419
Size: 36 210 650 248 2740
Fragments arranged by size:
2740 650 248 210 36
NgoMIV G'CCGG_C
Cuts at: 685 2063 685
Size: 1378 2506
NotI GC'GGCC_GC
Cuts at: 221 314 221
Size: 93 3791
NspI r_CATG'y
Cuts at: 937 1437 2344 937
Size: 500 907 2477
Fragments arranged by size:
2477 907 500
PasI CC'CwG_GG
Cuts at: 488 488
Size: 3884
PciI A'CATG_T
Cuts at: 2340 2340
Size: 3884
PflMI CCAn_nnn'nTGG
Cuts at: 213 657 213
Size: 444 3440
PmeI GTTT'AAAC
Cuts at: 883 883
Size: 3884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 346 378 3023 3055 346
Size: 32 2645 32 1175
Fragments arranged by size:
2645 1175 32 32
PpuMI rG'GwC_Cy
Cuts at: 468 492 468
Size: 24 3860
PspOMI G'GGCC_C
Cuts at: 349 349
Size: 3884
PstI C_TGCA'G
Cuts at: 434 931 1616 434
Size: 497 685 2702
Fragments arranged by size:
2702 685 497
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3884
PvuII CAG'CTG
Cuts at: 414 618 681 1309 1669 414
Size: 204 63 628 360 2629
Fragments arranged by size:
2629 628 360 204 63
RsrII CG'GwC_CG
Cuts at: 2079 2079
Size: 3884
SacI G_AGCT'C
Cuts at: 359 898 359
Size: 539 3345
SacII CC_GC'GG
Cuts at: 122 314 323 122
Size: 192 9 3683
Fragments arranged by size:
3683 192 9
SalI G'TCGA_C
Cuts at: 921 921
Size: 3884
SanDI GG'GwC_CC
Cuts at: 468 468
Size: 3884
SapI GCTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
SbfI CC_TGCA'GG
Cuts at: 931 931
Size: 3884
ScaI AGT'ACT
Cuts at: 3401 3401
Size: 3884
SfcI C'TryA_G
Cuts at: 33 430 927 1612 2605 2796 33
Size: 397 497 685 993 191 1121
Fragments arranged by size:
1121 993 685 497 397 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 286 286
Size: 3884
SfoI GGC'GCC
Cuts at: 420 456 666 1316 1564 420
Size: 36 210 650 248 2740
Fragments arranged by size:
2740 650 248 210 36
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
SgrAI Cr'CCGG_yG
Cuts at: 172 172
Size: 3884
SmaI CCC'GGG
Cuts at: 906 906
Size: 3884
SmlI C'TyrA_G
Cuts at: 1039 1954 2446 2708 1039
Size: 915 492 262 2215
Fragments arranged by size:
2215 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3884
SphI G_CATG'C
Cuts at: 937 1437 937
Size: 500 3384
StyI C'CwwG_G
Cuts at: 149 158 1018 1372 149
Size: 9 860 354 2661
Fragments arranged by size:
2661 860 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1467 1600 2059 2268 1467
Size: 133 459 209 3083
Fragments arranged by size:
3083 459 209 133
TatI w'GTAC_w
Cuts at: 746 3399 746
Size: 2653 1231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3401 3433 3401
Size: 32 3852
Tth111I GACn'n_nGTC
Cuts at: 1681 1681
Size: 3884
XbaI T'CTAG_A
Cuts at: 915 915
Size: 3884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 147 1379 147
Size: 1232 2652
XmaI C'CCGG_G
Cuts at: 904 904
Size: 3884
XmnI GAAnn'nnTTC
Cuts at: 505 505
Size: 3884
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspXI PsrI SexAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI