Restriction map for
pF1KB8215
(Circular) (Six-base) MAPSORT of: pF1KB8215.seq Check: 1252 from: 1 to: 3533
pF1KB8215 3533 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 571 571
Size: 3533
Acc65I G'GTAC_C
Cuts at: 549 549
Size: 3533
AclI AA'CG_TT
Cuts at: 3373 3373
Size: 3533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 469 1358 1790 2537 2805 277
Size: 192 889 432 747 268 1005
Fragments arranged by size:
1005 889 747 432 268 192
AflIII A'CryG_T
Cuts at: 885 1989 2966 885
Size: 1104 977 1452
Fragments arranged by size:
1452 1104 977
AgeI A'CCGG_T
Cuts at: 1921 1921
Size: 3533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 3533
AlwNI CAG_nnn'CTG
Cuts at: 237 838 2405 237
Size: 601 1567 1365
Fragments arranged by size:
1567 1365 601
ApaLI G'TGCA_C
Cuts at: 523 2303 523
Size: 1780 1753
ApoI r'AATT_y
Cuts at: 537 2886 3142 537
Size: 2349 256 928
Fragments arranged by size:
2349 928 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3533
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
AvaI C'yCGr_G
Cuts at: 553 553
Size: 3533
BamHI G'GATC_C
Cuts at: 558 558
Size: 3533
BanI G'GyrC_C
Cuts at: 549 963 1211 1246 549
Size: 414 248 35 2836
Fragments arranged by size:
2836 414 248 35
BanII G_rGCy'C
Cuts at: 276 547 1577 276
Size: 271 1030 2232
Fragments arranged by size:
2232 1030 271
BbeI G_GCGC'C
Cuts at: 967 1215 967
Size: 248 3285
BbsI GAAGACnn'nnnn_
Cuts at: 457 457
Size: 3533
BciVI GTATCCnnnnn_n'
Cuts at: 1431 2192 3533 1431
Size: 761 1341 1431
Fragments arranged by size:
1431 1341 761
BclI T'GATC_A
Cuts at: 1053 1053
Size: 3533
BglII A'GATC_T
Cuts at: 1048 3085 1048
Size: 2037 1496
BlpI GC'TnA_GC
Cuts at: 645 645
Size: 3533
Bme1580I G_kGCm'C
Cuts at: 527 1158 1251 2307 527
Size: 631 93 1056 1753
Fragments arranged by size:
1753 1056 631 93
BmrI ACTGGGnnnn_n'
Cuts at: 225 745 826 1161 3305 225
Size: 520 81 335 2144 453
Fragments arranged by size:
2144 520 453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 290 155
Size: 135 3398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1588 2080 2378 709
Size: 879 492 298 1864
Fragments arranged by size:
1864 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3237 3237
Size: 3533
BsaAI yAC'GTr
Cuts at: 886 886
Size: 3533
BsaBI GATnn'nnATC
Cuts at: 6 1047 3084 6
Size: 1041 2037 455
Fragments arranged by size:
2037 1041 455
BsaHI Gr'CG_yC
Cuts at: 964 1212 1914 3410 964
Size: 248 702 1496 1087
Fragments arranged by size:
1496 1087 702 248
BsaWI w'CCGG_w
Cuts at: 722 1243 1921 2195 2342 2753 722
Size: 521 678 274 147 411 1502
Fragments arranged by size:
1502 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 176 311 176
Size: 135 3398
BseYI C'CCAG_C
Cuts at: 311 958 1716 2293 2789 311
Size: 647 758 577 496 1055
Fragments arranged by size:
1055 758 647 577 496
BsiEI CG_ry'CG
Cuts at: 104 1075 1121 2329 104
Size: 971 46 1208 1308
Fragments arranged by size:
1308 1208 971 46
BsiHKAI G_wGCw'C
Cuts at: 276 428 527 547 1325 1515 2307 276
Size: 152 99 20 778 190 792 1502
Fragments arranged by size:
1502 792 778 190 152 99 20
Bsp1286I G_dGCh'C
Cuts at: 276 428 527 547 1158 1251 1325 1515 1577
Size: 152 99 20 611 93 74 190 62
Cuts at: 1577 2307 276
Size: 730 1502
Fragments arranged by size:
1502 730 611 190 152 99 93 74
62 20
BspEI T'CCGG_A
Cuts at: 2753 2753
Size: 3533
BspHI T'CATG_A
Cuts at: 189 2709 189
Size: 2520 1013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 583 797 1099 1480 583
Size: 214 302 381 2636
Fragments arranged by size:
2636 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1825 1879 3363 3528 50
Size: 1775 54 1484 165 55
Fragments arranged by size:
1775 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1445 1445
Size: 3533
BsrFI r'CCGG_y
Cuts at: 1531 1712 1921 1531
Size: 181 209 3143
Fragments arranged by size:
3143 209 181
BssSI C'ACGA_G
Cuts at: 458 1804 2162 458
Size: 1346 358 1829
Fragments arranged by size:
1829 1346 358
BstAPI GCAn_nnn'nTGC
Cuts at: 910 910
Size: 3533
BstBI TT'CG_AA
Cuts at: 1894 1894
Size: 3533
BstYI r'GATC_y
Cuts at: 1 443 558 1025 1048 1383 1629 2641 2727
Size: 442 115 467 23 335 246 1012 86
Cuts at: 2727 2739 3085 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 442 346 335 246 115
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1483 1664 1827 487
Size: 996 181 163 2193
Fragments arranged by size:
2193 996 181 163
BtsI GCAGTG_nn'
Cuts at: 776 776
Size: 3533
DraI TTT'AAA
Cuts at: 532 532
Size: 3533
DrdI GACnn_nn'nnGTC
Cuts at: 1239 2097 1239
Size: 858 2675
EaeI y'GGCC_r
Cuts at: 1118 1292 1683 1710 3055 1118
Size: 174 391 27 1345 1596
Fragments arranged by size:
1596 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1118 1118
Size: 3533
EarI CTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1741 2051 2197 3343 1741
Size: 310 146 1146 1931
Fragments arranged by size:
1931 1146 310 146
EcoICRI GAG'CTC
Cuts at: 274 545 274
Size: 271 3262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 277 290 469 1358 1790 2537 2805 155
Size: 122 13 179 889 432 747 268 883
Fragments arranged by size:
889 883 747 432 268 179 122 13
EcoO109I rG'GnC_Cy
Cuts at: 672 672
Size: 3533
EcoRI G'AATT_C
Cuts at: 537 537
Size: 3533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1552 1552
Size: 3533
FspI TGC'GCA
Cuts at: 1314 1314
Size: 3533
HaeII r_GCGC'y
Cuts at: 967 1215 2237 3216 967
Size: 248 1022 979 1284
Fragments arranged by size:
1284 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 171 203 275 307 1375 1405 1407 1437 171
Size: 32 72 32 1068 30 2 30 2267
Fragments arranged by size:
2267 1068 72 32 32 30 30 2
HincII GTy'rAC
Cuts at: 227 572 227
Size: 345 3188
KasI G'GCGC_C
Cuts at: 963 1211 963
Size: 248 3285
KpnI G_GTAC'C
Cuts at: 553 553
Size: 3533
MluI A'CGCG_T
Cuts at: 2966 2966
Size: 3533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2388 2204
Size: 184 3349
MscI TGG'CCA
Cuts at: 1294 1294
Size: 3533
MslI CAynn'nnrTG
Cuts at: 475 1522 1649 475
Size: 1047 127 2359
Fragments arranged by size:
2359 1047 127
MspA1I CmG'CkG
Cuts at: 644 958 1318 2331 2576 644
Size: 314 360 1013 245 1601
Fragments arranged by size:
1601 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1714 1714
Size: 3533
NarI GG'CG_CC
Cuts at: 964 1212 964
Size: 248 3285
NcoI C'CATG_G
Cuts at: 107 107
Size: 3533
NgoMIV G'CCGG_C
Cuts at: 1712 1712
Size: 3533
NspI r_CATG'y
Cuts at: 474 586 1086 1993 474
Size: 112 500 907 2014
Fragments arranged by size:
2014 907 500 112
PciI A'CATG_T
Cuts at: 1989 1989
Size: 3533
PfoI T'CCnGG_A
Cuts at: 267 267
Size: 3533
PmeI GTTT'AAAC
Cuts at: 532 532
Size: 3533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2672 2704 2672
Size: 32 3501
PstI C_TGCA'G
Cuts at: 374 580 1265 374
Size: 206 685 2642
Fragments arranged by size:
2642 685 206
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3533
PvuII CAG'CTG
Cuts at: 958 1318 958
Size: 360 3173
RsrII CG'GwC_CG
Cuts at: 1728 1728
Size: 3533
SacI G_AGCT'C
Cuts at: 276 547 276
Size: 271 3262
SalI G'TCGA_C
Cuts at: 570 570
Size: 3533
SapI GCTCTTCn'nnn_
Cuts at: 1556 1766 1556
Size: 210 3323
SbfI CC_TGCA'GG
Cuts at: 580 580
Size: 3533
ScaI AGT'ACT
Cuts at: 3050 3050
Size: 3533
SfcI C'TryA_G
Cuts at: 33 370 576 1261 2254 2445 33
Size: 337 206 685 993 191 1121
Fragments arranged by size:
1121 993 685 337 206 191
SfoI GGC'GCC
Cuts at: 965 1213 965
Size: 248 3285
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3533
SmaI CCC'GGG
Cuts at: 555 555
Size: 3533
SmlI C'TyrA_G
Cuts at: 688 1603 2095 2357 688
Size: 915 492 262 1864
Fragments arranged by size:
1864 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3533
SphI G_CATG'C
Cuts at: 474 586 1086 474
Size: 112 500 2921
Fragments arranged by size:
2921 500 112
StuI AGG'CCT
Cuts at: 307 307
Size: 3533
StyI C'CwwG_G
Cuts at: 107 332 667 1021 107
Size: 225 335 354 2619
Fragments arranged by size:
2619 354 335 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1116 1249 1708 1917 1116
Size: 133 459 209 2732
Fragments arranged by size:
2732 459 209 133
TatI w'GTAC_w
Cuts at: 3048 3048
Size: 3533
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3501
Tth111I GACn'n_nGTC
Cuts at: 1330 1330
Size: 3533
XbaI T'CTAG_A
Cuts at: 564 564
Size: 3533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1028 1028
Size: 3533
XmaI C'CCGG_G
Cuts at: 553 553
Size: 3533
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI