Restriction map for
pF1KB8217
(Circular) (Six-base) MAPSORT of: pF1KB8217.seq Check: 9974 from: 1 to: 3992
pF1KB8217 3992 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 222 222
Size: 3992
AccI GT'mk_AC
Cuts at: 956 1030 956
Size: 74 3918
Acc65I G'GTAC_C
Cuts at: 1008 1008
Size: 3992
AclI AA'CG_TT
Cuts at: 3832 3832
Size: 3992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1817 2249 2996 3264 1817
Size: 432 747 268 2545
Fragments arranged by size:
2545 747 432 268
AfeI AGC'GCT
Cuts at: 271 271
Size: 3992
AflIII A'CryG_T
Cuts at: 563 1344 2448 3425 563
Size: 781 1104 977 1130
Fragments arranged by size:
1130 1104 977 781
AgeI A'CCGG_T
Cuts at: 2380 2380
Size: 3992
AhdI GACnn_n'nnGTC
Cuts at: 671 671
Size: 3992
AlwNI CAG_nnn'CTG
Cuts at: 765 941 1297 2864 765
Size: 176 356 1567 1893
Fragments arranged by size:
1893 1567 356 176
ApaI G_GGCC'C
Cuts at: 129 301 906 129
Size: 172 605 3215
Fragments arranged by size:
3215 605 172
ApaLI G'TGCA_C
Cuts at: 2762 2762
Size: 3992
ApoI r'AATT_y
Cuts at: 996 3345 3601 996
Size: 2349 256 1387
Fragments arranged by size:
2349 1387 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
AvaI C'yCGr_G
Cuts at: 389 872 1012 389
Size: 483 140 3369
Fragments arranged by size:
3369 483 140
BamHI G'GATC_C
Cuts at: 1017 1017
Size: 3992
BanI G'GyrC_C
Cuts at: 210 252 700 1008 1422 1670 1705 210
Size: 42 448 308 414 248 35 2497
Fragments arranged by size:
2497 448 414 308 248 42 35
BanII G_rGCy'C
Cuts at: 129 301 650 734 845 906 1006 2036 129
Size: 172 349 84 111 61 100 1030 2085
Fragments arranged by size:
2085 1030 349 172 111 100 84 61
BbeI G_GCGC'C
Cuts at: 1426 1674 1426
Size: 248 3744
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3958
BciVI GTATCCnnnnn_n'
Cuts at: 1890 2651 3992 1890
Size: 761 1341 1890
Fragments arranged by size:
1890 1341 761
BclI T'GATC_A
Cuts at: 1512 1512
Size: 3992
BglI GCCn_nnn'nGGC
Cuts at: 332 332
Size: 3992
BglII A'GATC_T
Cuts at: 1507 3544 1507
Size: 2037 1955
BlpI GC'TnA_GC
Cuts at: 968 1104 968
Size: 136 3856
Bme1580I G_kGCm'C
Cuts at: 129 301 906 1617 1710 2766 129
Size: 172 605 711 93 1056 1355
Fragments arranged by size:
1355 1056 711 605 172 93
BmrI ACTGGGnnnn_n'
Cuts at: 123 358 839 1204 1285 1620 3764 123
Size: 235 481 365 81 335 2144 351
Fragments arranged by size:
2144 481 365 351 335 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 3992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 664 682 844 859 588
Size: 76 18 162 15 3721
Fragments arranged by size:
3721 162 76 18 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 486 703 1168 2047 2539 2837 306
Size: 180 217 465 879 492 298 1461
Fragments arranged by size:
1461 879 492 465 298 217 180
BsaI GGTCTCn'nnnn_
Cuts at: 454 658 3696 454
Size: 204 3038 750
Fragments arranged by size:
3038 750 204
BsaAI yAC'GTr
Cuts at: 564 1345 564
Size: 781 3211
BsaBI GATnn'nnATC
Cuts at: 6 1506 3543 6
Size: 1500 2037 455
Fragments arranged by size:
2037 1500 455
BsaHI Gr'CG_yC
Cuts at: 881 1423 1671 2373 3869 881
Size: 542 248 702 1496 1004
Fragments arranged by size:
1496 1004 702 542 248
BsaWI w'CCGG_w
Cuts at: 692 1181 1702 2380 2654 2801 3212 692
Size: 489 521 678 274 147 411 1472
Fragments arranged by size:
1472 678 521 489 411 274 147
BseYI C'CCAG_C
Cuts at: 1417 2175 2752 3248 1417
Size: 758 577 496 2161
Fragments arranged by size:
2161 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1534 1580 2788 104
Size: 1430 46 1208 1308
Fragments arranged by size:
1430 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 642 734 1006 1784 1974 2766 642
Size: 92 272 778 190 792 1868
Fragments arranged by size:
1868 792 778 272 190 92
Bsp1286I G_dGCh'C
Cuts at: 129 301 642 650 734 845 906 1006 1617
Size: 172 341 8 84 111 61 100 611
Cuts at: 1617 1710 1784 1974 2036 2766 129
Size: 93 74 190 62 730 1355
Fragments arranged by size:
1355 730 611 341 190 172 111 100
93 84 74 62 61 8
BspEI T'CCGG_A
Cuts at: 692 3212 692
Size: 2520 1472
BspHI T'CATG_A
Cuts at: 542 3168 542
Size: 2626 1366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 222 1042 1256 1558 1939 222
Size: 820 214 302 381 2275
Fragments arranged by size:
2275 820 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2284 2338 3822 3987 50
Size: 2234 54 1484 165 55
Fragments arranged by size:
2234 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1904 1904
Size: 3992
BsrFI r'CCGG_y
Cuts at: 372 512 852 1990 2171 2380 372
Size: 140 340 1138 181 209 1984
Fragments arranged by size:
1984 1138 340 209 181 140
BsrGI T'GTAC_A
Cuts at: 566 566
Size: 3992
BssHII G'CGCG_C
Cuts at: 115 115
Size: 3992
BssSI C'ACGA_G
Cuts at: 2263 2621 2263
Size: 358 3634
BstAPI GCAn_nnn'nTGC
Cuts at: 1369 1369
Size: 3992
BstBI TT'CG_AA
Cuts at: 2353 2353
Size: 3992
BstYI r'GATC_y
Cuts at: 1 1017 1484 1507 1842 2088 3100 3186 3198
Size: 1016 467 23 335 246 1012 86 12
Cuts at: 3198 3544 1
Size: 346 449
Fragments arranged by size:
1016 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1942 2123 2286 1942
Size: 181 163 3648
Fragments arranged by size:
3648 181 163
BtsI GCAGTG_nn'
Cuts at: 744 1235 744
Size: 491 3501
DraI TTT'AAA
Cuts at: 991 991
Size: 3992
DrdI GACnn_nn'nnGTC
Cuts at: 1698 2556 1698
Size: 858 3134
EaeI y'GGCC_r
Cuts at: 510 767 885 1577 1751 2142 2169 3514 510
Size: 257 118 692 174 391 27 1345 988
Fragments arranged by size:
1345 988 692 391 257 174 118 27
EagI C'GGCC_G
Cuts at: 1577 1577
Size: 3992
EarI CTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2200 2510 2656 3802 2200
Size: 310 146 1146 2390
Fragments arranged by size:
2390 1146 310 146
EcoICRI GAG'CTC
Cuts at: 732 1004 732
Size: 272 3720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 664 682 844 859 1817 2249 2996 3264
Size: 76 18 162 15 958 432 747 268
Cuts at: 3264 588
Size: 1316
Fragments arranged by size:
1316 958 747 432 268 162 76 18
15
EcoNI CCTnn'n_nnAGG
Cuts at: 802 802
Size: 3992
EcoO109I rG'GnC_Cy
Cuts at: 125 408 554 762 795 1131 125
Size: 283 146 208 33 336 2986
Fragments arranged by size:
2986 336 283 208 146 33
EcoRI G'AATT_C
Cuts at: 996 996
Size: 3992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3992
FspI TGC'GCA
Cuts at: 1773 1773
Size: 3992
HaeII r_GCGC'y
Cuts at: 273 1426 1674 2696 3675 273
Size: 1153 248 1022 979 590
Fragments arranged by size:
1153 1022 979 590 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 437 469 657 689 1834 1864 1866 1896 437
Size: 32 188 32 1145 30 2 30 2533
Fragments arranged by size:
2533 1145 188 32 32 30 30 2
HincII GTy'rAC
Cuts at: 957 1031 957
Size: 74 3918
KasI G'GCGC_C
Cuts at: 1422 1670 1422
Size: 248 3744
KpnI G_GTAC'C
Cuts at: 1012 1012
Size: 3992
MluI A'CGCG_T
Cuts at: 3425 3425
Size: 3992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 335 2663 2847 221
Size: 114 2328 184 1366
Fragments arranged by size:
2328 1366 184 114
MscI TGG'CCA
Cuts at: 769 1753 769
Size: 984 3008
MslI CAynn'nnrTG
Cuts at: 541 1981 2108 541
Size: 1440 127 2425
Fragments arranged by size:
2425 1440 127
MspA1I CmG'CkG
Cuts at: 122 1103 1417 1777 2790 3035 122
Size: 981 314 360 1013 245 1079
Fragments arranged by size:
1079 1013 981 360 314 245
NaeI GCC'GGC
Cuts at: 374 514 854 2173 374
Size: 140 340 1319 2193
Fragments arranged by size:
2193 1319 340 140
NarI GG'CG_CC
Cuts at: 1423 1671 1423
Size: 248 3744
NgoMIV G'CCGG_C
Cuts at: 372 512 852 2171 372
Size: 140 340 1319 2193
Fragments arranged by size:
2193 1319 340 140
NspI r_CATG'y
Cuts at: 540 585 1045 1545 2452 540
Size: 45 460 500 907 2080
Fragments arranged by size:
2080 907 500 460 45
PasI CC'CwG_GG
Cuts at: 263 941 263
Size: 678 3314
PciI A'CATG_T
Cuts at: 2448 2448
Size: 3992
PflMI CCAn_nnn'nTGG
Cuts at: 765 765
Size: 3992
PmeI GTTT'AAAC
Cuts at: 991 991
Size: 3992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 3960
PpuMI rG'GwC_Cy
Cuts at: 408 554 795 408
Size: 146 241 3605
Fragments arranged by size:
3605 241 146
PspOMI G'GGCC_C
Cuts at: 125 297 902 125
Size: 172 605 3215
Fragments arranged by size:
3215 605 172
PstI C_TGCA'G
Cuts at: 219 739 1039 1724 219
Size: 520 300 685 2487
Fragments arranged by size:
2487 685 520 300
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3992
PvuII CAG'CTG
Cuts at: 1417 1777 1417
Size: 360 3632
RsrII CG'GwC_CG
Cuts at: 2187 2187
Size: 3992
SacI G_AGCT'C
Cuts at: 734 1006 734
Size: 272 3720
SalI G'TCGA_C
Cuts at: 955 1029 955
Size: 74 3918
SanDI GG'GwC_CC
Cuts at: 554 554
Size: 3992
SapI GCTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
SbfI CC_TGCA'GG
Cuts at: 1039 1039
Size: 3992
ScaI AGT'ACT
Cuts at: 3509 3509
Size: 3992
SfcI C'TryA_G
Cuts at: 33 215 735 857 1035 1720 2713 2904 33
Size: 182 520 122 178 685 993 191 1121
Fragments arranged by size:
1121 993 685 520 191 182 178 122
SfoI GGC'GCC
Cuts at: 1424 1672 1424
Size: 248 3744
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
SmaI CCC'GGG
Cuts at: 391 874 1014 391
Size: 483 140 3369
Fragments arranged by size:
3369 483 140
SmlI C'TyrA_G
Cuts at: 321 501 718 1147 2062 2554 2816 321
Size: 180 217 429 915 492 262 1497
Fragments arranged by size:
1497 915 492 429 262 217 180
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3992
SphI G_CATG'C
Cuts at: 1045 1545 1045
Size: 500 3492
SrfI GCCC'GGGC
Cuts at: 391 391
Size: 3992
StyI C'CwwG_G
Cuts at: 1126 1480 1126
Size: 354 3638
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 478 1575 1708 2167 2376 478
Size: 1097 133 459 209 2094
Fragments arranged by size:
2094 1097 459 209 133
TatI w'GTAC_w
Cuts at: 566 584 3507 566
Size: 18 2923 1051
Fragments arranged by size:
2923 1051 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 178 210 220 252 3509 3541 178
Size: 32 10 32 3257 32 629
Fragments arranged by size:
3257 629 32 32 32 10
Tth111I GACn'n_nGTC
Cuts at: 1789 1789
Size: 3992
XbaI T'CTAG_A
Cuts at: 1023 1023
Size: 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 505 1487 505
Size: 982 3010
XmaI C'CCGG_G
Cuts at: 389 872 1012 389
Size: 483 140 3369
Fragments arranged by size:
3369 483 140
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI
BsgI BsiWI BsmI BsmBI BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
XmnI ZraI