Restriction map for
pF1KB8223
(Circular) (Six-base) MAPSORT of: pF1KB8223.seq Check: 4268 from: 1 to: 3929
pF1KB8223 3929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 543 543
Size: 3929
AccI GT'mk_AC
Cuts at: 967 967
Size: 3929
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 3769 3769
Size: 3929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1754 2186 2933 3201 1754
Size: 432 747 268 2482
Fragments arranged by size:
2482 747 432 268
AflIII A'CryG_T
Cuts at: 608 1281 2385 3362 608
Size: 673 1104 977 1175
Fragments arranged by size:
1175 1104 977 673
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AlwNI CAG_nnn'CTG
Cuts at: 1234 2801 1234
Size: 1567 2362
ApaLI G'TGCA_C
Cuts at: 2699 2699
Size: 3929
ApoI r'AATT_y
Cuts at: 933 3282 3538 933
Size: 2349 256 1324
Fragments arranged by size:
2349 1324 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 949 949
Size: 3929
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 945 1359 1607 1642 945
Size: 414 248 35 3232
Fragments arranged by size:
3232 414 248 35
BanII G_rGCy'C
Cuts at: 596 943 1973 596
Size: 347 1030 2552
Fragments arranged by size:
2552 1030 347
BbeI G_GCGC'C
Cuts at: 1363 1611 1363
Size: 248 3681
BbvCI CC'TCA_GC
Cuts at: 749 749
Size: 3929
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglI GCCn_nnn'nGGC
Cuts at: 894 894
Size: 3929
BglII A'GATC_T
Cuts at: 1444 3481 1444
Size: 2037 1892
BlpI GC'TnA_GC
Cuts at: 674 716 770 1041 674
Size: 42 54 271 3562
Fragments arranged by size:
3562 271 54 42
Bme1580I G_kGCm'C
Cuts at: 209 1554 1647 2703 209
Size: 1345 93 1056 1435
Fragments arranged by size:
1435 1345 1056 93
BmgBI CAC'GTC
Cuts at: 609 609
Size: 3929
BmrI ACTGGGnnnn_n'
Cuts at: 1141 1222 1557 3701 1141
Size: 81 335 2144 1369
Fragments arranged by size:
2144 1369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 561 774 2296 561
Size: 213 1522 2194
Fragments arranged by size:
2194 1522 213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 536 895 531
Size: 5 359 3565
Fragments arranged by size:
3565 359 5
Bpu10I CC'TnA_GC
Cuts at: 153 749 153
Size: 596 3333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1105 1984 2476 2774 472
Size: 633 879 492 298 1627
Fragments arranged by size:
1627 879 633 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 549 3633 549
Size: 3084 845
BsaAI yAC'GTr
Cuts at: 328 1282 328
Size: 954 2975
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 1360 1608 2310 3806 1360
Size: 248 702 1496 1483
Fragments arranged by size:
1496 1483 702 248
BsaWI w'CCGG_w
Cuts at: 1118 1639 2317 2591 2738 3149 1118
Size: 521 678 274 147 411 1898
Fragments arranged by size:
1898 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 158 188 158
Size: 30 3899
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 898 898
Size: 3929
BseYI C'CCAG_C
Cuts at: 202 1354 2112 2689 3185 202
Size: 1152 758 577 496 946
Fragments arranged by size:
1152 946 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 262
Size: 3929
BsiEI CG_ry'CG
Cuts at: 104 1471 1517 2725 104
Size: 1367 46 1208 1308
Fragments arranged by size:
1367 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 908 943 1721 1911 2703 908
Size: 35 778 190 792 2134
Fragments arranged by size:
2134 792 778 190 35
Bsp1286I G_dGCh'C
Cuts at: 209 596 908 943 1554 1647 1721 1911 1973
Size: 387 312 35 611 93 74 190 62
Cuts at: 1973 2703 209
Size: 730 1435
Fragments arranged by size:
1435 730 611 387 312 190 93 74
62 35
BspEI T'CCGG_A
Cuts at: 3149 3149
Size: 3929
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 543 893 979 1193 1495 1876 543
Size: 350 86 214 302 381 2596
Fragments arranged by size:
2596 381 350 302 214 86
BsrBI CCG'CTC
Cuts at: 50 2221 2275 3759 3924 50
Size: 2171 54 1484 165 55
Fragments arranged by size:
2171 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 1927 2108 2317 1927
Size: 181 209 3539
Fragments arranged by size:
3539 209 181
BssSI C'ACGA_G
Cuts at: 2200 2558 2200
Size: 358 3571
BstAPI GCAn_nnn'nTGC
Cuts at: 903 1306 903
Size: 403 3526
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstXI CCAn_nnnn'nTGG
Cuts at: 669 702 789 669
Size: 33 87 3809
Fragments arranged by size:
3809 87 33
BstYI r'GATC_y
Cuts at: 1 482 954 1421 1444 1779 2025 3037 3123
Size: 481 472 467 23 335 246 1012 86
Cuts at: 3123 3135 3481 1
Size: 12 346 449
Fragments arranged by size:
1012 481 472 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 234 283 107
Size: 127 49 3753
Fragments arranged by size:
3753 127 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 831 1879 2060 2223 831
Size: 1048 181 163 2537
Fragments arranged by size:
2537 1048 181 163
BtsI GCAGTG_nn'
Cuts at: 379 794 1172 379
Size: 415 378 3136
Fragments arranged by size:
3136 415 378
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2493 1635
Size: 858 3071
EaeI y'GGCC_r
Cuts at: 734 791 1514 1688 2079 2106 3451 734
Size: 57 723 174 391 27 1345 1212
Fragments arranged by size:
1345 1212 723 391 174 57 27
EagI C'GGCC_G
Cuts at: 1514 1514
Size: 3929
EarI CTCTTCn'nnn_
Cuts at: 397 1952 2162 397
Size: 1555 210 2164
Fragments arranged by size:
2164 1555 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2137 2447 2593 3739 2137
Size: 310 146 1146 2327
Fragments arranged by size:
2327 1146 310 146
EcoICRI GAG'CTC
Cuts at: 941 941
Size: 3929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 536 895 1754 2186 2933 3201 531
Size: 5 359 859 432 747 268 1259
Fragments arranged by size:
1259 859 747 432 359 268 5
EcoO109I rG'GnC_Cy
Cuts at: 1068 1068
Size: 3929
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FspI TGC'GCA
Cuts at: 385 823 1710 385
Size: 438 887 2604
Fragments arranged by size:
2604 887 438
HaeII r_GCGC'y
Cuts at: 1363 1611 2633 3612 1363
Size: 248 1022 979 1680
Fragments arranged by size:
1680 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 203 235 509 527 541 559 595 627 1771
Size: 32 274 18 14 18 36 32 1144
Cuts at: 1771 1801 1803 1833 203
Size: 30 2 30 2299
Fragments arranged by size:
2299 1144 274 36 32 32 30 30
18 18 14 2
HincII GTy'rAC
Cuts at: 372 968 372
Size: 596 3333
KasI G'GCGC_C
Cuts at: 1359 1607 1359
Size: 248 3681
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2784 2600
Size: 184 3745
MscI TGG'CCA
Cuts at: 793 1690 793
Size: 897 3032
MslI CAynn'nnrTG
Cuts at: 1918 2045 1918
Size: 127 3802
MspA1I CmG'CkG
Cuts at: 769 892 915 1040 1354 1714 2727 2972 769
Size: 123 23 125 314 360 1013 245 1726
Fragments arranged by size:
1726 1013 360 314 245 125 123 23
NaeI GCC'GGC
Cuts at: 2110 2110
Size: 3929
NarI GG'CG_CC
Cuts at: 1360 1608 1360
Size: 248 3681
NcoI C'CATG_G
Cuts at: 107 107
Size: 3929
NgoMIV G'CCGG_C
Cuts at: 2108 2108
Size: 3929
NspI r_CATG'y
Cuts at: 982 1482 2389 982
Size: 500 907 2522
Fragments arranged by size:
2522 907 500
PciI A'CATG_T
Cuts at: 2385 2385
Size: 3929
PfoI T'CCnGG_A
Cuts at: 599 599
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3897
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 865 897 865
Size: 32 3897
PstI C_TGCA'G
Cuts at: 801 840 976 1661 801
Size: 39 136 685 3069
Fragments arranged by size:
3069 685 136 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 769 915 1354 1714 769
Size: 146 439 360 2984
Fragments arranged by size:
2984 439 360 146
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 943 943
Size: 3929
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SfcI C'TryA_G
Cuts at: 33 797 836 972 1657 2650 2841 33
Size: 764 39 136 685 993 191 1121
Fragments arranged by size:
1121 993 764 685 191 136 39
SfoI GGC'GCC
Cuts at: 1361 1609 1361
Size: 248 3681
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 951 951
Size: 3929
SmlI C'TyrA_G
Cuts at: 487 1084 1999 2491 2753 487
Size: 597 915 492 262 1663
Fragments arranged by size:
1663 915 597 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
StyI C'CwwG_G
Cuts at: 107 761 1063 1417 107
Size: 654 302 354 2619
Fragments arranged by size:
2619 654 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 242 544 1512 1645 2104 2313 242
Size: 302 968 133 459 209 1858
Fragments arranged by size:
1858 968 459 302 209 133
TatI w'GTAC_w
Cuts at: 3444 3444
Size: 3929
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 158 190 371 403 3446 3478 158
Size: 32 181 32 3043 32 609
Fragments arranged by size:
3043 609 181 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 702 1424 702
Size: 722 3207
XmaI C'CCGG_G
Cuts at: 949 949
Size: 3929
XmnI GAAnn'nnTTC
Cuts at: 640 640
Size: 3929
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI