Restriction map for
pF1KB8224
(Circular) (Six-base) MAPSORT of: pF1KB8224.seq Check: 3143 from: 1 to: 3872
pF1KB8224 3872 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 378 378
Size: 3872
AccI GT'mk_AC
Cuts at: 910 910
Size: 3872
Acc65I G'GTAC_C
Cuts at: 888 888
Size: 3872
AclI AA'CG_TT
Cuts at: 3712 3712
Size: 3872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1697 2129 2876 3144 1697
Size: 432 747 268 2425
Fragments arranged by size:
2425 747 432 268
AflIII A'CryG_T
Cuts at: 1224 2328 3305 1224
Size: 1104 977 1791
Fragments arranged by size:
1791 1104 977
AgeI A'CCGG_T
Cuts at: 2260 2260
Size: 3872
AleI CACnn'nnGTG
Cuts at: 520 520
Size: 3872
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 445 446 477 478 445
Size: 1 31 1 3839
Fragments arranged by size:
3839 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 862 1177 2744 862
Size: 315 1567 1990
Fragments arranged by size:
1990 1567 315
ApaI G_GGCC'C
Cuts at: 668 668
Size: 3872
ApaLI G'TGCA_C
Cuts at: 2642 2642
Size: 3872
ApoI r'AATT_y
Cuts at: 876 3225 3481 876
Size: 2349 256 1267
Fragments arranged by size:
2349 1267 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
AvaI C'yCGr_G
Cuts at: 308 812 892 308
Size: 504 80 3288
Fragments arranged by size:
3288 504 80
BamHI G'GATC_C
Cuts at: 897 897
Size: 3872
BanI G'GyrC_C
Cuts at: 323 888 1302 1550 1585 323
Size: 565 414 248 35 2610
Fragments arranged by size:
2610 565 414 248 35
BanII G_rGCy'C
Cuts at: 278 668 886 1916 278
Size: 390 218 1030 2234
Fragments arranged by size:
2234 1030 390 218
BbeI G_GCGC'C
Cuts at: 327 1306 1554 327
Size: 979 248 2645
Fragments arranged by size:
2645 979 248
BbsI GAAGACnn'nnnn_
Cuts at: 770 770
Size: 3872
BciVI GTATCCnnnnn_n'
Cuts at: 1770 2531 3872 1770
Size: 761 1341 1770
Fragments arranged by size:
1770 1341 761
BclI T'GATC_A
Cuts at: 1392 1392
Size: 3872
BglI GCCn_nnn'nGGC
Cuts at: 672 672
Size: 3872
BglII A'GATC_T
Cuts at: 1387 3424 1387
Size: 2037 1835
BlpI GC'TnA_GC
Cuts at: 984 984
Size: 3872
Bme1580I G_kGCm'C
Cuts at: 550 668 1497 1590 2646 550
Size: 118 829 93 1056 1776
Fragments arranged by size:
1776 1056 829 118 93
BmrI ACTGGGnnnn_n'
Cuts at: 662 1084 1165 1500 3644 662
Size: 422 81 335 2144 890
Fragments arranged by size:
2144 890 422 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 177 452 2239 177
Size: 275 1787 1810
Fragments arranged by size:
1810 1787 275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 250 547 572 713 238
Size: 12 297 25 141 3397
Fragments arranged by size:
3397 297 141 25 12
Bpu10I CC'TnA_GC
Cuts at: 270 270
Size: 3872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1927 2419 2717 1048
Size: 879 492 298 2203
Fragments arranged by size:
2203 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3576 3576
Size: 3872
BsaAI yAC'GTr
Cuts at: 1225 1225
Size: 3872
BsaBI GATnn'nnATC
Cuts at: 6 1386 3423 6
Size: 1380 2037 455
Fragments arranged by size:
2037 1380 455
BsaHI Gr'CG_yC
Cuts at: 324 705 1303 1551 2253 3749 324
Size: 381 598 248 702 1496 447
Fragments arranged by size:
1496 702 598 447 381 248
BsaWI w'CCGG_w
Cuts at: 1061 1582 2260 2534 2681 3092 1061
Size: 521 678 274 147 411 1841
Fragments arranged by size:
1841 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 235 265 235
Size: 30 3842
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 3872
BseYI C'CCAG_C
Cuts at: 648 855 1297 2055 2632 3128 648
Size: 207 442 758 577 496 1392
Fragments arranged by size:
1392 758 577 496 442 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 611 766 445
Size: 166 155 3551
Fragments arranged by size:
3551 166 155
BsiEI CG_ry'CG
Cuts at: 104 1414 1460 2668 104
Size: 1310 46 1208 1308
Fragments arranged by size:
1310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 886 1664 1854 2646 886
Size: 778 190 792 2112
Fragments arranged by size:
2112 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 226 226
Size: 3872
Bsp1286I G_dGCh'C
Cuts at: 278 550 668 886 1497 1590 1664 1854 1916
Size: 272 118 218 611 93 74 190 62
Cuts at: 1916 2646 278
Size: 730 1504
Fragments arranged by size:
1504 730 611 272 218 190 118 93
74 62
BspEI T'CCGG_A
Cuts at: 3092 3092
Size: 3872
BspHI T'CATG_A
Cuts at: 467 3048 467
Size: 2581 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 378 922 1136 1438 1819 378
Size: 544 214 302 381 2431
Fragments arranged by size:
2431 544 381 302 214
BsrBI CCG'CTC
Cuts at: 50 399 2164 2218 3702 3867 50
Size: 349 1765 54 1484 165 55
Fragments arranged by size:
1765 1484 349 165 55 54
BsrDI GCAATG_nn'
Cuts at: 576 1784 576
Size: 1208 2664
BsrFI r'CCGG_y
Cuts at: 1870 2051 2260 1870
Size: 181 209 3482
Fragments arranged by size:
3482 209 181
BssSI C'ACGA_G
Cuts at: 2143 2501 2143
Size: 358 3514
BstAPI GCAn_nnn'nTGC
Cuts at: 718 1249 718
Size: 531 3341
BstBI TT'CG_AA
Cuts at: 2233 2233
Size: 3872
BstXI CCAn_nnnn'nTGG
Cuts at: 114 525 114
Size: 411 3461
BstYI r'GATC_y
Cuts at: 1 897 1364 1387 1722 1968 2980 3066 3078
Size: 896 467 23 335 246 1012 86 12
Cuts at: 3078 3424 1
Size: 346 449
Fragments arranged by size:
1012 896 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 134 143 694 107
Size: 27 9 551 3285
Fragments arranged by size:
3285 551 27 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1822 2003 2166 1822
Size: 181 163 3528
Fragments arranged by size:
3528 181 163
BtsI GCAGTG_nn'
Cuts at: 1115 1115
Size: 3872
DraI TTT'AAA
Cuts at: 871 871
Size: 3872
DrdI GACnn_nn'nnGTC
Cuts at: 1578 2436 1578
Size: 858 3014
EaeI y'GGCC_r
Cuts at: 207 848 1457 1631 2022 2049 3394 207
Size: 641 609 174 391 27 1345 685
Fragments arranged by size:
1345 685 641 609 391 174 27
EagI C'GGCC_G
Cuts at: 1457 1457
Size: 3872
EarI CTCTTCn'nnn_
Cuts at: 287 293 673 1895 2105 287
Size: 6 380 1222 210 2054
Fragments arranged by size:
2054 1222 380 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 690 2080 2390 2536 3682 690
Size: 1390 310 146 1146 880
Fragments arranged by size:
1390 1146 880 310 146
EcoICRI GAG'CTC
Cuts at: 884 884
Size: 3872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 250 547 572 713 1697 2129 2876 3144
Size: 12 297 25 141 984 432 747 268
Cuts at: 3144 238
Size: 966
Fragments arranged by size:
984 966 747 432 297 268 141 25
12
EcoO109I rG'GnC_Cy
Cuts at: 402 664 789 1011 402
Size: 262 125 222 3263
Fragments arranged by size:
3263 262 222 125
EcoRI G'AATT_C
Cuts at: 876 876
Size: 3872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3872
FspI TGC'GCA
Cuts at: 1653 1653
Size: 3872
HaeII r_GCGC'y
Cuts at: 169 327 372 1306 1554 2576 3555 169
Size: 158 45 934 248 1022 979 486
Fragments arranged by size:
1022 979 934 486 248 158 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 589 621 1714 1744 1746 1776 235
Size: 32 322 32 1093 30 2 30 2331
Fragments arranged by size:
2331 1093 322 32 32 30 30 2
HincII GTy'rAC
Cuts at: 911 911
Size: 3872
KasI G'GCGC_C
Cuts at: 323 1302 1550 323
Size: 979 248 2645
Fragments arranged by size:
2645 979 248
KpnI G_GTAC'C
Cuts at: 892 892
Size: 3872
MluI A'CGCG_T
Cuts at: 3305 3305
Size: 3872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2727 2543
Size: 184 3688
MscI TGG'CCA
Cuts at: 209 850 1633 209
Size: 641 783 2448
Fragments arranged by size:
2448 783 641
MslI CAynn'nnrTG
Cuts at: 472 520 1861 1988 472
Size: 48 1341 127 2356
Fragments arranged by size:
2356 1341 127 48
MspA1I CmG'CkG
Cuts at: 330 983 1297 1657 2670 2915 330
Size: 653 314 360 1013 245 1287
Fragments arranged by size:
1287 1013 653 360 314 245
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 3872
NarI GG'CG_CC
Cuts at: 324 1303 1551 324
Size: 979 248 2645
Fragments arranged by size:
2645 979 248
NcoI C'CATG_G
Cuts at: 107 134 107
Size: 27 3845
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 3872
NspI r_CATG'y
Cuts at: 925 1425 2332 925
Size: 500 907 2465
Fragments arranged by size:
2465 907 500
PciI A'CATG_T
Cuts at: 2328 2328
Size: 3872
PflMI CCAn_nnn'nTGG
Cuts at: 216 700 216
Size: 484 3388
PfoI T'CCnGG_A
Cuts at: 215 278 215
Size: 63 3809
PmeI GTTT'AAAC
Cuts at: 871 871
Size: 3872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3840
PpuMI rG'GwC_Cy
Cuts at: 402 402
Size: 3872
PspOMI G'GGCC_C
Cuts at: 664 664
Size: 3872
PstI C_TGCA'G
Cuts at: 449 919 1604 449
Size: 470 685 2717
Fragments arranged by size:
2717 685 470
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3872
PvuII CAG'CTG
Cuts at: 1297 1657 1297
Size: 360 3512
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 3872
SacI G_AGCT'C
Cuts at: 886 886
Size: 3872
SalI G'TCGA_C
Cuts at: 909 909
Size: 3872
SapI GCTCTTCn'nnn_
Cuts at: 1895 2105 1895
Size: 210 3662
SbfI CC_TGCA'GG
Cuts at: 919 919
Size: 3872
ScaI AGT'ACT
Cuts at: 3389 3389
Size: 3872
SfcI C'TryA_G
Cuts at: 33 445 915 1600 2593 2784 33
Size: 412 470 685 993 191 1121
Fragments arranged by size:
1121 993 685 470 412 191
SfoI GGC'GCC
Cuts at: 325 1304 1552 325
Size: 979 248 2645
Fragments arranged by size:
2645 979 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
SmaI CCC'GGG
Cuts at: 310 894 310
Size: 584 3288
SmlI C'TyrA_G
Cuts at: 1027 1942 2434 2696 1027
Size: 915 492 262 2203
Fragments arranged by size:
2203 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3872
SphI G_CATG'C
Cuts at: 925 1425 925
Size: 500 3372
StyI C'CwwG_G
Cuts at: 107 134 1006 1360 107
Size: 27 872 354 2619
Fragments arranged by size:
2619 872 354 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1455 1588 2047 2256 1455
Size: 133 459 209 3071
Fragments arranged by size:
3071 459 209 133
TatI w'GTAC_w
Cuts at: 3387 3387
Size: 3872
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 3840
Tth111I GACn'n_nGTC
Cuts at: 603 1669 603
Size: 1066 2806
XbaI T'CTAG_A
Cuts at: 903 903
Size: 3872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 1367 141
Size: 1226 2646
XmaI C'CCGG_G
Cuts at: 308 892 308
Size: 584 3288
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI