Restriction map for
pF1KB8227
(Circular) (Six-base) MAPSORT of: pF1KB8227.seq Check: 6478 from: 1 to: 3668
pF1KB8227 3668 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 706 706
Size: 3668
Acc65I G'GTAC_C
Cuts at: 684 684
Size: 3668
AclI AA'CG_TT
Cuts at: 3508 3508
Size: 3668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1493 1925 2672 2940 1493
Size: 432 747 268 2221
Fragments arranged by size:
2221 747 432 268
AflIII A'CryG_T
Cuts at: 1020 2124 3101 1020
Size: 1104 977 1587
Fragments arranged by size:
1587 1104 977
AgeI A'CCGG_T
Cuts at: 2056 2056
Size: 3668
AlwNI CAG_nnn'CTG
Cuts at: 385 973 2540 385
Size: 588 1567 1513
Fragments arranged by size:
1567 1513 588
ApaLI G'TGCA_C
Cuts at: 2438 2438
Size: 3668
ApoI r'AATT_y
Cuts at: 672 3021 3277 672
Size: 2349 256 1063
Fragments arranged by size:
2349 1063 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3668
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
AvaI C'yCGr_G
Cuts at: 490 688 490
Size: 198 3470
BamHI G'GATC_C
Cuts at: 693 693
Size: 3668
BanI G'GyrC_C
Cuts at: 684 1098 1346 1381 684
Size: 414 248 35 2971
Fragments arranged by size:
2971 414 248 35
BanII G_rGCy'C
Cuts at: 682 1712 682
Size: 1030 2638
BbeI G_GCGC'C
Cuts at: 1102 1350 1102
Size: 248 3420
BciVI GTATCCnnnnn_n'
Cuts at: 1566 2327 3668 1566
Size: 761 1341 1566
Fragments arranged by size:
1566 1341 761
BclI T'GATC_A
Cuts at: 1188 1188
Size: 3668
BglI GCCn_nnn'nGGC
Cuts at: 118 118
Size: 3668
BglII A'GATC_T
Cuts at: 1183 3220 1183
Size: 2037 1631
BlpI GC'TnA_GC
Cuts at: 780 780
Size: 3668
Bme1580I G_kGCm'C
Cuts at: 1293 1386 2442 1293
Size: 93 1056 2519
Fragments arranged by size:
2519 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 242 880 961 1296 3440 242
Size: 638 81 335 2144 470
Fragments arranged by size:
2144 638 470 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 3668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 545
Size: 3668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1723 2215 2513 844
Size: 879 492 298 1999
Fragments arranged by size:
1999 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3372 3372
Size: 3668
BsaAI yAC'GTr
Cuts at: 1021 1021
Size: 3668
BsaBI GATnn'nnATC
Cuts at: 6 1182 3219 6
Size: 1176 2037 455
Fragments arranged by size:
2037 1176 455
BsaHI Gr'CG_yC
Cuts at: 473 1099 1347 2049 3545 473
Size: 626 248 702 1496 596
Fragments arranged by size:
1496 702 626 596 248
BsaWI w'CCGG_w
Cuts at: 367 614 857 1378 2056 2330 2477 2888 367
Size: 247 243 521 678 274 147 411 1147
Fragments arranged by size:
1147 678 521 411 274 247 243 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 266 296 266
Size: 30 3638
BseYI C'CCAG_C
Cuts at: 150 253 1093 1851 2428 2924 150
Size: 103 840 758 577 496 894
Fragments arranged by size:
894 840 758 577 496 103
BsiEI CG_ry'CG
Cuts at: 104 1210 1256 2464 104
Size: 1106 46 1208 1308
Fragments arranged by size:
1308 1208 1106 46
BsiHKAI G_wGCw'C
Cuts at: 682 1460 1650 2442 682
Size: 778 190 792 1908
Fragments arranged by size:
1908 792 778 190
BsmI GAATG_Cn'
Cuts at: 624 624
Size: 3668
Bsp1286I G_dGCh'C
Cuts at: 682 1293 1386 1460 1650 1712 2442 682
Size: 611 93 74 190 62 730 1908
Fragments arranged by size:
1908 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2888 2888
Size: 3668
BspHI T'CATG_A
Cuts at: 2844 2844
Size: 3668
BspMI ACCTGCnnnn'nnnn_
Cuts at: 718 932 1234 1615 718
Size: 214 302 381 2771
Fragments arranged by size:
2771 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1960 2014 3498 3663 50
Size: 1910 54 1484 165 55
Fragments arranged by size:
1910 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 542 1580 542
Size: 1038 2630
BsrFI r'CCGG_y
Cuts at: 1666 1847 2056 1666
Size: 181 209 3278
Fragments arranged by size:
3278 209 181
BssSI C'ACGA_G
Cuts at: 1939 2297 1939
Size: 358 3310
BstAPI GCAn_nnn'nTGC
Cuts at: 1045 1045
Size: 3668
BstBI TT'CG_AA
Cuts at: 2029 2029
Size: 3668
BstYI r'GATC_y
Cuts at: 1 693 1160 1183 1518 1764 2776 2862 2874
Size: 692 467 23 335 246 1012 86 12
Cuts at: 2874 3220 1
Size: 346 449
Fragments arranged by size:
1012 692 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 114 518 107
Size: 7 404 3257
Fragments arranged by size:
3257 404 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1618 1799 1962 1618
Size: 181 163 3324
Fragments arranged by size:
3324 181 163
BtsI GCAGTG_nn'
Cuts at: 227 435 911 227
Size: 208 476 2984
Fragments arranged by size:
2984 476 208
DraI TTT'AAA
Cuts at: 667 667
Size: 3668
DrdI GACnn_nn'nnGTC
Cuts at: 1374 2232 1374
Size: 858 2810
EaeI y'GGCC_r
Cuts at: 1253 1427 1818 1845 3190 1253
Size: 174 391 27 1345 1731
Fragments arranged by size:
1731 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1253 1253
Size: 3668
EarI CTCTTCn'nnn_
Cuts at: 145 458 1691 1901 145
Size: 313 1233 210 1912
Fragments arranged by size:
1912 1233 313 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1876 2186 2332 3478 1876
Size: 310 146 1146 2066
Fragments arranged by size:
2066 1146 310 146
EcoICRI GAG'CTC
Cuts at: 680 680
Size: 3668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 1493 1925 2672 2940 545
Size: 948 432 747 268 1273
Fragments arranged by size:
1273 948 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 807 807
Size: 3668
EcoRI G'AATT_C
Cuts at: 672 672
Size: 3668
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 585 1687 585
Size: 1102 2566
FspI TGC'GCA
Cuts at: 1449 1449
Size: 3668
HaeII r_GCGC'y
Cuts at: 133 1102 1350 2372 3351 133
Size: 969 248 1022 979 450
Fragments arranged by size:
1022 979 969 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 324 356 1510 1540 1542 1572 324
Size: 32 1154 30 2 30 2420
Fragments arranged by size:
2420 1154 32 30 30 2
HincII GTy'rAC
Cuts at: 707 707
Size: 3668
KasI G'GCGC_C
Cuts at: 1098 1346 1098
Size: 248 3420
KpnI G_GTAC'C
Cuts at: 688 688
Size: 3668
MluI A'CGCG_T
Cuts at: 3101 3101
Size: 3668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2523 2339
Size: 184 3484
MscI TGG'CCA
Cuts at: 1429 1429
Size: 3668
MslI CAynn'nnrTG
Cuts at: 617 1657 1784 617
Size: 1040 127 2501
Fragments arranged by size:
2501 1040 127
MspA1I CmG'CkG
Cuts at: 116 637 779 1093 1453 2466 2711 116
Size: 521 142 314 360 1013 245 1073
Fragments arranged by size:
1073 1013 521 360 314 245 142
NaeI GCC'GGC
Cuts at: 1849 1849
Size: 3668
NarI GG'CG_CC
Cuts at: 1099 1347 1099
Size: 248 3420
NcoI C'CATG_G
Cuts at: 107 107
Size: 3668
NgoMIV G'CCGG_C
Cuts at: 1847 1847
Size: 3668
NspI r_CATG'y
Cuts at: 721 1221 2128 721
Size: 500 907 2261
Fragments arranged by size:
2261 907 500
PciI A'CATG_T
Cuts at: 2124 2124
Size: 3668
PmeI GTTT'AAAC
Cuts at: 667 667
Size: 3668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 2807
Size: 32 3636
PstI C_TGCA'G
Cuts at: 715 1400 715
Size: 685 2983
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3668
PvuII CAG'CTG
Cuts at: 637 1093 1453 637
Size: 456 360 2852
Fragments arranged by size:
2852 456 360
RsrII CG'GwC_CG
Cuts at: 1863 1863
Size: 3668
SacI G_AGCT'C
Cuts at: 682 682
Size: 3668
SacII CC_GC'GG
Cuts at: 117 117
Size: 3668
SalI G'TCGA_C
Cuts at: 705 705
Size: 3668
SapI GCTCTTCn'nnn_
Cuts at: 145 1691 1901 145
Size: 1546 210 1912
Fragments arranged by size:
1912 1546 210
SbfI CC_TGCA'GG
Cuts at: 715 715
Size: 3668
ScaI AGT'ACT
Cuts at: 3185 3185
Size: 3668
SfcI C'TryA_G
Cuts at: 33 711 1396 2389 2580 33
Size: 678 685 993 191 1121
Fragments arranged by size:
1121 993 685 678 191
SfoI GGC'GCC
Cuts at: 1100 1348 1100
Size: 248 3420
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3668
SmaI CCC'GGG
Cuts at: 492 690 492
Size: 198 3470
SmlI C'TyrA_G
Cuts at: 823 1738 2230 2492 823
Size: 915 492 262 1999
Fragments arranged by size:
1999 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3668
SphI G_CATG'C
Cuts at: 721 1221 721
Size: 500 3168
StyI C'CwwG_G
Cuts at: 107 802 1156 107
Size: 695 354 2619
Fragments arranged by size:
2619 695 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 142 339 1251 1384 1843 2052 142
Size: 197 912 133 459 209 1758
Fragments arranged by size:
1758 912 459 209 197 133
TatI w'GTAC_w
Cuts at: 3183 3183
Size: 3668
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 346 378 3185 3217 346
Size: 32 2807 32 797
Fragments arranged by size:
2807 797 32 32
Tth111I GACn'n_nGTC
Cuts at: 1465 1465
Size: 3668
XbaI T'CTAG_A
Cuts at: 699 699
Size: 3668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1163 1163
Size: 3668
XmaI C'CCGG_G
Cuts at: 490 688 490
Size: 198 3470
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI