Restriction map for
pF1KB8229
(Circular) (Six-base) MAPSORT of: pF1KB8229.seq Check: 5658 from: 1 to: 3617
pF1KB8229 3617 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 655 655
Size: 3617
Acc65I G'GTAC_C
Cuts at: 633 633
Size: 3617
AclI AA'CG_TT
Cuts at: 3457 3457
Size: 3617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1442 1874 2621 2889 1442
Size: 432 747 268 2170
Fragments arranged by size:
2170 747 432 268
AfeI AGC'GCT
Cuts at: 433 433
Size: 3617
AflIII A'CryG_T
Cuts at: 969 2073 3050 969
Size: 1104 977 1536
Fragments arranged by size:
1536 1104 977
AgeI A'CCGG_T
Cuts at: 2005 2005
Size: 3617
AlwNI CAG_nnn'CTG
Cuts at: 922 2489 922
Size: 1567 2050
ApaI G_GGCC'C
Cuts at: 178 200 178
Size: 22 3595
ApaLI G'TGCA_C
Cuts at: 2387 2387
Size: 3617
ApoI r'AATT_y
Cuts at: 621 2970 3226 621
Size: 2349 256 1012
Fragments arranged by size:
2349 1012 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3617
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
AvaI C'yCGr_G
Cuts at: 637 637
Size: 3617
BamHI G'GATC_C
Cuts at: 642 642
Size: 3617
BanI G'GyrC_C
Cuts at: 405 633 1047 1295 1330 405
Size: 228 414 248 35 2692
Fragments arranged by size:
2692 414 248 228 35
BanII G_rGCy'C
Cuts at: 178 200 377 449 631 1661 178
Size: 22 177 72 182 1030 2134
Fragments arranged by size:
2134 1030 182 177 72 22
BbeI G_GCGC'C
Cuts at: 409 1051 1299 409
Size: 642 248 2727
Fragments arranged by size:
2727 642 248
BbsI GAAGACnn'nnnn_
Cuts at: 152 549 152
Size: 397 3220
BciVI GTATCCnnnnn_n'
Cuts at: 1515 2276 3617 1515
Size: 761 1341 1515
Fragments arranged by size:
1515 1341 761
BclI T'GATC_A
Cuts at: 1137 1137
Size: 3617
BglII A'GATC_T
Cuts at: 1132 3169 1132
Size: 2037 1580
BlpI GC'TnA_GC
Cuts at: 729 729
Size: 3617
Bme1580I G_kGCm'C
Cuts at: 178 200 517 1242 1335 2391 178
Size: 22 317 725 93 1056 1404
Fragments arranged by size:
1404 1056 725 317 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 829 910 1245 3389 829
Size: 81 335 2144 1057
Fragments arranged by size:
2144 1057 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 525 135
Size: 390 3227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1672 2164 2462 793
Size: 879 492 298 1948
Fragments arranged by size:
1948 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3321 3321
Size: 3617
BsaAI yAC'GTr
Cuts at: 970 970
Size: 3617
BsaBI GATnn'nnATC
Cuts at: 6 1131 3168 6
Size: 1125 2037 455
Fragments arranged by size:
2037 1125 455
BsaHI Gr'CG_yC
Cuts at: 248 406 1048 1296 1998 3494 248
Size: 158 642 248 702 1496 371
Fragments arranged by size:
1496 702 642 371 248 158
BsaWI w'CCGG_w
Cuts at: 314 449 806 1327 2005 2279 2426 2837 314
Size: 135 357 521 678 274 147 411 1094
Fragments arranged by size:
1094 678 521 411 357 274 147 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 444 474 444
Size: 30 3587
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 385
Size: 3617
BseYI C'CCAG_C
Cuts at: 178 298 376 1042 1800 2377 2873 178
Size: 120 78 666 758 577 496 922
Fragments arranged by size:
922 758 666 577 496 120 78
BsiEI CG_ry'CG
Cuts at: 104 1159 1205 2413 104
Size: 1055 46 1208 1308
Fragments arranged by size:
1308 1208 1055 46
BsiHKAI G_wGCw'C
Cuts at: 449 631 1409 1599 2391 449
Size: 182 778 190 792 1675
Fragments arranged by size:
1675 792 778 190 182
Bsp1286I G_dGCh'C
Cuts at: 178 200 377 449 517 631 1242 1335 1409
Size: 22 177 72 68 114 611 93 74
Cuts at: 1409 1599 1661 2391 178
Size: 190 62 730 1404
Fragments arranged by size:
1404 730 611 190 177 114 93 74
72 68 62 22
BspEI T'CCGG_A
Cuts at: 314 449 2837 314
Size: 135 2388 1094
Fragments arranged by size:
2388 1094 135
BspHI T'CATG_A
Cuts at: 2793 2793
Size: 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 667 881 1183 1564 667
Size: 214 302 381 2720
Fragments arranged by size:
2720 381 302 214
BsrBI CCG'CTC
Cuts at: 50 395 1909 1963 3447 3612 50
Size: 345 1514 54 1484 165 55
Fragments arranged by size:
1514 1484 345 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1529 1529
Size: 3617
BsrFI r'CCGG_y
Cuts at: 1615 1796 2005 1615
Size: 181 209 3227
Fragments arranged by size:
3227 209 181
BssSI C'ACGA_G
Cuts at: 1888 2246 1888
Size: 358 3259
BstAPI GCAn_nnn'nTGC
Cuts at: 994 994
Size: 3617
BstBI TT'CG_AA
Cuts at: 1978 1978
Size: 3617
BstYI r'GATC_y
Cuts at: 1 116 311 642 1109 1132 1467 1713 2725
Size: 115 195 331 467 23 335 246 1012
Cuts at: 2725 2811 2823 3169 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 331 246 195
115 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 201 201
Size: 3617
BtgI C'CryG_G
Cuts at: 338 338
Size: 3617
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 205 1567 1748 1911 205
Size: 1362 181 163 1911
Fragments arranged by size:
1911 1362 181 163
BtsI GCAGTG_nn'
Cuts at: 860 860
Size: 3617
DraI TTT'AAA
Cuts at: 616 616
Size: 3617
DrdI GACnn_nn'nnGTC
Cuts at: 1323 2181 1323
Size: 858 2759
EaeI y'GGCC_r
Cuts at: 438 1202 1376 1767 1794 3139 438
Size: 764 174 391 27 1345 916
Fragments arranged by size:
1345 916 764 391 174 27
EagI C'GGCC_G
Cuts at: 1202 1202
Size: 3617
EarI CTCTTCn'nnn_
Cuts at: 499 1640 1850 499
Size: 1141 210 2266
Fragments arranged by size:
2266 1141 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 588 1825 2135 2281 3427 588
Size: 1237 310 146 1146 778
Fragments arranged by size:
1237 1146 778 310 146
EcoICRI GAG'CTC
Cuts at: 447 629 447
Size: 182 3435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 525 1442 1874 2621 2889 135
Size: 390 917 432 747 268 863
Fragments arranged by size:
917 863 747 432 390 268
EcoO109I rG'GnC_Cy
Cuts at: 168 175 197 228 756 168
Size: 7 22 31 528 3029
Fragments arranged by size:
3029 528 31 22 7
EcoRI G'AATT_C
Cuts at: 621 621
Size: 3617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1636 1636
Size: 3617
FspI TGC'GCA
Cuts at: 1398 1398
Size: 3617
HaeII r_GCGC'y
Cuts at: 299 409 435 1051 1299 2321 3300 299
Size: 110 26 616 248 1022 979 616
Fragments arranged by size:
1022 979 616 616 248 110 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 171 184 216 303 335 1459 1489 1491
Size: 32 13 32 87 32 1124 30 2
Cuts at: 1491 1521 139
Size: 30 2235
Fragments arranged by size:
2235 1124 87 32 32 32 30 30
13 2
HincII GTy'rAC
Cuts at: 656 656
Size: 3617
KasI G'GCGC_C
Cuts at: 405 1047 1295 405
Size: 642 248 2727
Fragments arranged by size:
2727 642 248
KpnI G_GTAC'C
Cuts at: 637 637
Size: 3617
MluI A'CGCG_T
Cuts at: 3050 3050
Size: 3617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2288 2472 2288
Size: 184 3433
MscI TGG'CCA
Cuts at: 1378 1378
Size: 3617
MslI CAynn'nnrTG
Cuts at: 1606 1733 1606
Size: 127 3490
MspA1I CmG'CkG
Cuts at: 340 545 728 1042 1402 2415 2660 340
Size: 205 183 314 360 1013 245 1297
Fragments arranged by size:
1297 1013 360 314 245 205 183
NaeI GCC'GGC
Cuts at: 1798 1798
Size: 3617
NarI GG'CG_CC
Cuts at: 406 1048 1296 406
Size: 642 248 2727
Fragments arranged by size:
2727 642 248
NgoMIV G'CCGG_C
Cuts at: 1796 1796
Size: 3617
NspI r_CATG'y
Cuts at: 670 1170 2077 670
Size: 500 907 2210
Fragments arranged by size:
2210 907 500
PciI A'CATG_T
Cuts at: 2073 2073
Size: 3617
PflMI CCAn_nnn'nTGG
Cuts at: 419 419
Size: 3617
PmeI GTTT'AAAC
Cuts at: 616 616
Size: 3617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2788 2756
Size: 32 3585
PshAI GACnn'nnGTC
Cuts at: 251 553 251
Size: 302 3315
PspOMI G'GGCC_C
Cuts at: 174 196 174
Size: 22 3595
PstI C_TGCA'G
Cuts at: 162 664 1349 162
Size: 502 685 2430
Fragments arranged by size:
2430 685 502
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3617
PvuII CAG'CTG
Cuts at: 545 1042 1402 545
Size: 497 360 2760
Fragments arranged by size:
2760 497 360
RsrII CG'GwC_CG
Cuts at: 1812 1812
Size: 3617
SacI G_AGCT'C
Cuts at: 449 631 449
Size: 182 3435
SacII CC_GC'GG
Cuts at: 341 341
Size: 3617
SalI G'TCGA_C
Cuts at: 654 654
Size: 3617
SapI GCTCTTCn'nnn_
Cuts at: 499 1640 1850 499
Size: 1141 210 2266
Fragments arranged by size:
2266 1141 210
SbfI CC_TGCA'GG
Cuts at: 664 664
Size: 3617
ScaI AGT'ACT
Cuts at: 3134 3134
Size: 3617
SfcI C'TryA_G
Cuts at: 33 158 660 1345 2338 2529 33
Size: 125 502 685 993 191 1121
Fragments arranged by size:
1121 993 685 502 191 125
SfoI GGC'GCC
Cuts at: 407 1049 1297 407
Size: 642 248 2727
Fragments arranged by size:
2727 642 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
SmaI CCC'GGG
Cuts at: 639 639
Size: 3617
SmlI C'TyrA_G
Cuts at: 772 1687 2179 2441 772
Size: 915 492 262 1948
Fragments arranged by size:
1948 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3617
SphI G_CATG'C
Cuts at: 670 1170 670
Size: 500 3117
StuI AGG'CCT
Cuts at: 236 236
Size: 3617
StyI C'CwwG_G
Cuts at: 751 1105 751
Size: 354 3263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1200 1333 1792 2001 1200
Size: 133 459 209 2816
Fragments arranged by size:
2816 459 209 133
TatI w'GTAC_w
Cuts at: 3132 3132
Size: 3617
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 444 476 3134 3166 444
Size: 32 2658 32 895
Fragments arranged by size:
2658 895 32 32
Tth111I GACn'n_nGTC
Cuts at: 1414 1414
Size: 3617
XbaI T'CTAG_A
Cuts at: 648 648
Size: 3617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 239 1112 239
Size: 873 2744
XmaI C'CCGG_G
Cuts at: 637 637
Size: 3617
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI