Restriction map for
pF1KB8230
(Circular) (Six-base) MAPSORT of: pF1KB8230.seq Check: 1082 from: 1 to: 4151
pF1KB8230 4151 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 525 525
Size: 4151
AccI GT'mk_AC
Cuts at: 1118 1189 1118
Size: 71 4080
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 1079 1976 2408 3155 3423 379
Size: 700 897 432 747 268 1107
Fragments arranged by size:
1107 897 747 700 432 268
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 1456 3023 1456
Size: 1567 2584
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 1155 3504 3760 1155
Size: 2349 256 1546
Fragments arranged by size:
2349 1546 256
AseI AT'TA_AT
Cuts at: 20 257 20
Size: 237 3914
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 127 800 1171 127
Size: 673 371 3107
Fragments arranged by size:
3107 673 371
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 1089 1167 1581 1829 1864 1089
Size: 78 414 248 35 3376
Fragments arranged by size:
3376 414 248 78 35
BanII G_rGCy'C
Cuts at: 1165 2195 1165
Size: 1030 3121
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglII A'GATC_T
Cuts at: 1666 3703 1666
Size: 2037 2114
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 1776 1869 2925 1776
Size: 93 1056 3002
Fragments arranged by size:
3002 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 872 1363 1444 1779 3923 872
Size: 491 81 335 2144 1100
Fragments arranged by size:
2144 1100 491 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 165 847 2518 165
Size: 682 1671 1798
Fragments arranged by size:
1798 1671 682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 4151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1062 1327 2206 2698 2996 1062
Size: 265 879 492 298 2217
Fragments arranged by size:
2217 879 492 298 265
BsaI GGTCTCn'nnnn_
Cuts at: 1118 3855 1118
Size: 2737 1414
BsaAI yAC'GTr
Cuts at: 346 1504 346
Size: 1158 2993
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 772 1115 1582 1830 2532 4028 772
Size: 343 467 248 702 1496 895
Fragments arranged by size:
1496 895 702 467 343 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 743 762 773 792 103
Size: 30 610 19 11 19 3462
Fragments arranged by size:
3462 610 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 743 800 863 1051 127
Size: 616 57 63 188 3227
Fragments arranged by size:
3227 616 188 63 57
BseYI C'CCAG_C
Cuts at: 273 705 987 1576 2334 2911 3407 273
Size: 432 282 589 758 577 496 1017
Fragments arranged by size:
1017 758 589 577 496 432 282
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1165 1943 2133 2925 1165
Size: 778 190 792 2391
Fragments arranged by size:
2391 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1165 1776 1869 1943 2133 2195 2925 1165
Size: 611 93 74 190 62 730 2391
Fragments arranged by size:
2391 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 131 525 1201 1415 1717 2098 131
Size: 394 676 214 302 381 2184
Fragments arranged by size:
2184 676 394 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BssSI C'ACGA_G
Cuts at: 764 2422 2780 764
Size: 1658 358 2135
Fragments arranged by size:
2135 1658 358
BstAPI GCAn_nnn'nTGC
Cuts at: 177 1528 177
Size: 1351 2800
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 1135 1135
Size: 4151
BstYI r'GATC_y
Cuts at: 1 1176 1643 1666 2001 2247 3259 3345 3357
Size: 1175 467 23 335 246 1012 86 12
Cuts at: 3357 3703 1
Size: 346 449
Fragments arranged by size:
1175 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 935 1394 935
Size: 459 3692
ClaI AT'CG_AT
Cuts at: 245 245
Size: 4151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1048 1083 1048
Size: 35 4116
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 390 1857 2715 390
Size: 1467 858 1826
Fragments arranged by size:
1826 1467 858
EaeI y'GGCC_r
Cuts at: 742 1736 1910 2301 2328 3673 742
Size: 994 174 391 27 1345 1220
Fragments arranged by size:
1345 1220 994 391 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 431 816 1058 2174 2384 431
Size: 385 242 1116 210 2198
Fragments arranged by size:
2198 1116 385 242 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 154 2359 2669 2815 3961 154
Size: 2205 310 146 1146 344
Fragments arranged by size:
2205 1146 344 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 595 1079 1976 2408 3155 3423 379
Size: 216 484 897 432 747 268 1107
Fragments arranged by size:
1107 897 747 484 432 268 216
EcoO109I rG'GnC_Cy
Cuts at: 1290 1290
Size: 4151
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 336 2170 336
Size: 1834 2317
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 1585 1833 2855 3834 1585
Size: 248 1022 979 1902
Fragments arranged by size:
1902 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 762 794 1013 1045 1993 2023 2025 2055 762
Size: 32 219 32 948 30 2 30 2858
Fragments arranged by size:
2858 948 219 32 32 30 30 2
HincII GTy'rAC
Cuts at: 620 1190 620
Size: 570 3581
HindIII A'AGCT_T
Cuts at: 524 524
Size: 4151
HpaI GTT'AAC
Cuts at: 620 620
Size: 4151
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 729 2822 3006 729
Size: 2093 184 1874
Fragments arranged by size:
2093 1874 184
MscI TGG'CCA
Cuts at: 1912 1912
Size: 4151
MslI CAynn'nnrTG
Cuts at: 247 476 502 2140 2267 247
Size: 229 26 1638 127 2131
Fragments arranged by size:
2131 1638 229 127 26
MspA1I CmG'CkG
Cuts at: 1262 1576 1936 2949 3194 1262
Size: 314 360 1013 245 2219
Fragments arranged by size:
2219 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NdeI CA'TA_TG
Cuts at: 1134 1134
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 547 1204 1704 2611 547
Size: 657 500 907 2087
Fragments arranged by size:
2087 907 657 500
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PfoI T'CCnGG_A
Cuts at: 611 611
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PmlI CAC'GTG
Cuts at: 346 346
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PsiI TTA'TAA
Cuts at: 584 584
Size: 4151
PstI C_TGCA'G
Cuts at: 522 1198 1883 522
Size: 676 685 2790
Fragments arranged by size:
2790 685 676
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1576 1936 1576
Size: 360 3791
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 466 3668 466
Size: 3202 949
SfcI C'TryA_G
Cuts at: 33 518 978 1119 1194 1879 2872 3063 33
Size: 485 460 141 75 685 993 191 1121
Fragments arranged by size:
1121 993 685 485 460 191 141 75
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 1077 1306 2221 2713 2975 1077
Size: 229 915 492 262 2253
Fragments arranged by size:
2253 915 492 262 229
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StyI C'CwwG_G
Cuts at: 107 399 428 1285 1639 107
Size: 292 29 857 354 2619
Fragments arranged by size:
2619 857 354 292 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1867 2326 2535 1734
Size: 133 459 209 3350
Fragments arranged by size:
3350 459 209 133
TatI w'GTAC_w
Cuts at: 239 464 3666 239
Size: 225 3202 724
Fragments arranged by size:
3202 724 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 269 301 3668 3700 269
Size: 32 3367 32 720
Fragments arranged by size:
3367 720 32 32
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
XmnI GAAnn'nnTTC
Cuts at: 206 466 206
Size: 260 3891
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI