Restriction map for
pF1KB8237
(Circular) (Six-base) MAPSORT of: pF1KB8237.seq Check: 8087 from: 1 to: 3890
pF1KB8237 3890 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 928 928
Size: 3890
Acc65I G'GTAC_C
Cuts at: 906 906
Size: 3890
AclI AA'CG_TT
Cuts at: 3730 3730
Size: 3890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 325 442 1715 2147 2894 3162 200
Size: 125 117 1273 432 747 268 928
Fragments arranged by size:
1273 928 747 432 268 125 117
AflIII A'CryG_T
Cuts at: 1242 2346 3323 1242
Size: 1104 977 1809
Fragments arranged by size:
1809 1104 977
AgeI A'CCGG_T
Cuts at: 2278 2278
Size: 3890
AhdI GACnn_n'nnGTC
Cuts at: 378 378
Size: 3890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 173 883 173
Size: 710 3180
AlwNI CAG_nnn'CTG
Cuts at: 217 1195 2762 217
Size: 978 1567 1345
Fragments arranged by size:
1567 1345 978
ApaLI G'TGCA_C
Cuts at: 2660 2660
Size: 3890
ApoI r'AATT_y
Cuts at: 894 3243 3499 894
Size: 2349 256 1285
Fragments arranged by size:
2349 1285 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3890
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
AvaI C'yCGr_G
Cuts at: 737 910 737
Size: 173 3717
BamHI G'GATC_C
Cuts at: 915 915
Size: 3890
BanI G'GyrC_C
Cuts at: 544 867 906 1320 1568 1603 544
Size: 323 39 414 248 35 2831
Fragments arranged by size:
2831 414 323 248 39 35
BanII G_rGCy'C
Cuts at: 904 1934 904
Size: 1030 2860
BbeI G_GCGC'C
Cuts at: 1324 1572 1324
Size: 248 3642
BbsI GAAGACnn'nnnn_
Cuts at: 137 188 137
Size: 51 3839
BciVI GTATCCnnnnn_n'
Cuts at: 799 1788 2549 3890 799
Size: 989 761 1341 799
Fragments arranged by size:
1341 989 799 761
BclI T'GATC_A
Cuts at: 1410 1410
Size: 3890
BglI GCCn_nnn'nGGC
Cuts at: 733 733
Size: 3890
BglII A'GATC_T
Cuts at: 1405 3442 1405
Size: 2037 1853
BlpI GC'TnA_GC
Cuts at: 1002 1002
Size: 3890
Bme1580I G_kGCm'C
Cuts at: 872 1515 1608 2664 872
Size: 643 93 1056 2098
Fragments arranged by size:
2098 1056 643 93
BmgBI CAC'GTC
Cuts at: 645 645
Size: 3890
BmrI ACTGGGnnnn_n'
Cuts at: 1102 1183 1518 3662 1102
Size: 81 335 2144 1330
Fragments arranged by size:
2144 1330 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 3890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 655 556
Size: 99 3791
Bpu10I CC'TnA_GC
Cuts at: 248 248
Size: 3890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1066 1945 2437 2735 829
Size: 237 879 492 298 1984
Fragments arranged by size:
1984 879 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 511 3594 511
Size: 3083 807
BsaAI yAC'GTr
Cuts at: 711 1243 711
Size: 532 3358
BsaBI GATnn'nnATC
Cuts at: 6 1404 3441 6
Size: 1398 2037 455
Fragments arranged by size:
2037 1398 455
BsaHI Gr'CG_yC
Cuts at: 679 1321 1569 2271 3767 679
Size: 642 248 702 1496 802
Fragments arranged by size:
1496 802 702 642 248
BsaWI w'CCGG_w
Cuts at: 267 809 1079 1600 2278 2552 2699 3110 267
Size: 542 270 521 678 274 147 411 1047
Fragments arranged by size:
1047 678 542 521 411 274 270 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 208
Size: 3890
BseYI C'CCAG_C
Cuts at: 119 146 1315 2073 2650 3146 119
Size: 27 1169 758 577 496 863
Fragments arranged by size:
1169 863 758 577 496 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 225
Size: 3890
BsiEI CG_ry'CG
Cuts at: 104 1432 1478 2686 104
Size: 1328 46 1208 1308
Fragments arranged by size:
1328 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 904 1682 1872 2664 904
Size: 778 190 792 2130
Fragments arranged by size:
2130 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 872 904 1515 1608 1682 1872 1934 2664 872
Size: 32 611 93 74 190 62 730 2098
Fragments arranged by size:
2098 730 611 190 93 74 62 32
BspEI T'CCGG_A
Cuts at: 3110 3110
Size: 3890
BspHI T'CATG_A
Cuts at: 3066 3066
Size: 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 940 1154 1456 1837 940
Size: 214 302 381 2993
Fragments arranged by size:
2993 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2182 2236 3720 3885 50
Size: 2132 54 1484 165 55
Fragments arranged by size:
2132 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1802 1802
Size: 3890
BsrFI r'CCGG_y
Cuts at: 329 360 1888 2069 2278 329
Size: 31 1528 181 209 1941
Fragments arranged by size:
1941 1528 209 181 31
BssSI C'ACGA_G
Cuts at: 2161 2519 2161
Size: 358 3532
BstAPI GCAn_nnn'nTGC
Cuts at: 1267 1267
Size: 3890
BstBI TT'CG_AA
Cuts at: 2251 2251
Size: 3890
BstYI r'GATC_y
Cuts at: 1 915 1382 1405 1740 1986 2998 3084 3096
Size: 914 467 23 335 246 1012 86 12
Cuts at: 3096 3442 1
Size: 346 449
Fragments arranged by size:
1012 914 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 881 881
Size: 3890
BtgI C'CryG_G
Cuts at: 107 794 107
Size: 687 3203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1840 2021 2184 1840
Size: 181 163 3546
Fragments arranged by size:
3546 181 163
BtsI GCAGTG_nn'
Cuts at: 682 1133 682
Size: 451 3439
DraI TTT'AAA
Cuts at: 889 889
Size: 3890
DrdI GACnn_nn'nnGTC
Cuts at: 220 451 1596 2454 220
Size: 231 1145 858 1656
Fragments arranged by size:
1656 1145 858 231
EaeI y'GGCC_r
Cuts at: 362 593 1475 1649 2040 2067 3412 362
Size: 231 882 174 391 27 1345 840
Fragments arranged by size:
1345 882 840 391 231 174 27
EagI C'GGCC_G
Cuts at: 1475 1475
Size: 3890
EarI CTCTTCn'nnn_
Cuts at: 856 1913 2123 856
Size: 1057 210 2623
Fragments arranged by size:
2623 1057 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 489 2098 2408 2554 3700 489
Size: 1609 310 146 1146 679
Fragments arranged by size:
1609 1146 679 310 146
EcoICRI GAG'CTC
Cuts at: 902 902
Size: 3890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 325 442 556 655 1715 2147 2894 3162
Size: 125 117 114 99 1060 432 747 268
Cuts at: 3162 200
Size: 928
Fragments arranged by size:
1060 928 747 432 268 125 117 114
99
EcoO109I rG'GnC_Cy
Cuts at: 174 1029 174
Size: 855 3035
EcoRI G'AATT_C
Cuts at: 894 894
Size: 3890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1909 1909
Size: 3890
FspI TGC'GCA
Cuts at: 1671 1671
Size: 3890
HaeII r_GCGC'y
Cuts at: 584 746 1324 1572 2594 3573 584
Size: 162 578 248 1022 979 901
Fragments arranged by size:
1022 979 901 578 248 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 1732 1762 1764 1794 364
Size: 32 1336 30 2 30 2460
Fragments arranged by size:
2460 1336 32 30 30 2
HincII GTy'rAC
Cuts at: 456 929 456
Size: 473 3417
KasI G'GCGC_C
Cuts at: 1320 1568 1320
Size: 248 3642
KpnI G_GTAC'C
Cuts at: 910 910
Size: 3890
MluI A'CGCG_T
Cuts at: 3323 3323
Size: 3890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2561 2745 2561
Size: 184 3706
MscI TGG'CCA
Cuts at: 595 1651 595
Size: 1056 2834
MslI CAynn'nnrTG
Cuts at: 781 1879 2006 781
Size: 1098 127 2665
Fragments arranged by size:
2665 1098 127
MspA1I CmG'CkG
Cuts at: 420 874 1001 1315 1675 2688 2933 420
Size: 454 127 314 360 1013 245 1377
Fragments arranged by size:
1377 1013 454 360 314 245 127
NaeI GCC'GGC
Cuts at: 362 2071 362
Size: 1709 2181
NarI GG'CG_CC
Cuts at: 1321 1569 1321
Size: 248 3642
NcoI C'CATG_G
Cuts at: 107 107
Size: 3890
NgoMIV G'CCGG_C
Cuts at: 360 2069 360
Size: 1709 2181
NspI r_CATG'y
Cuts at: 943 1443 2350 943
Size: 500 907 2483
Fragments arranged by size:
2483 907 500
PasI CC'CwG_GG
Cuts at: 801 801
Size: 3890
PciI A'CATG_T
Cuts at: 2346 2346
Size: 3890
PflMI CCAn_nnn'nTGG
Cuts at: 561 561
Size: 3890
PmeI GTTT'AAAC
Cuts at: 889 889
Size: 3890
PmlI CAC'GTG
Cuts at: 711 711
Size: 3890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3858
PpuMI rG'GwC_Cy
Cuts at: 174 174
Size: 3890
PstI C_TGCA'G
Cuts at: 129 204 937 1622 129
Size: 75 733 685 2397
Fragments arranged by size:
2397 733 685 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3890
PvuII CAG'CTG
Cuts at: 420 1315 1675 420
Size: 895 360 2635
Fragments arranged by size:
2635 895 360
RsrII CG'GwC_CG
Cuts at: 2085 2085
Size: 3890
SacI G_AGCT'C
Cuts at: 904 904
Size: 3890
SalI G'TCGA_C
Cuts at: 927 927
Size: 3890
SanDI GG'GwC_CC
Cuts at: 174 174
Size: 3890
SapI GCTCTTCn'nnn_
Cuts at: 856 1913 2123 856
Size: 1057 210 2623
Fragments arranged by size:
2623 1057 210
SbfI CC_TGCA'GG
Cuts at: 937 937
Size: 3890
ScaI AGT'ACT
Cuts at: 3407 3407
Size: 3890
SexAI A'CCwGG_T
Cuts at: 863 863
Size: 3890
SfcI C'TryA_G
Cuts at: 33 125 200 933 1618 2611 2802 33
Size: 92 75 733 685 993 191 1121
Fragments arranged by size:
1121 993 733 685 191 92 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 733 733
Size: 3890
SfoI GGC'GCC
Cuts at: 1322 1570 1322
Size: 248 3642
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
SmaI CCC'GGG
Cuts at: 912 912
Size: 3890
SmlI C'TyrA_G
Cuts at: 844 1045 1960 2452 2714 844
Size: 201 915 492 262 2020
Fragments arranged by size:
2020 915 492 262 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3890
SphI G_CATG'C
Cuts at: 943 1443 943
Size: 500 3390
StuI AGG'CCT
Cuts at: 727 727
Size: 3890
StyI C'CwwG_G
Cuts at: 107 1024 1378 107
Size: 917 354 2619
Fragments arranged by size:
2619 917 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1473 1606 2065 2274 1473
Size: 133 459 209 3089
Fragments arranged by size:
3089 459 209 133
TatI w'GTAC_w
Cuts at: 3405 3405
Size: 3890
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 3858
Tth111I GACn'n_nGTC
Cuts at: 1687 1687
Size: 3890
XbaI T'CTAG_A
Cuts at: 921 921
Size: 3890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1385 1385
Size: 3890
XmaI C'CCGG_G
Cuts at: 910 910
Size: 3890
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI