Restriction map for
pF1KB8240
(Circular) (Six-base) MAPSORT of: pF1KB8240.seq Check: 9704 from: 1 to: 3845
pF1KB8240 3845 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 883 883
Size: 3845
Acc65I G'GTAC_C
Cuts at: 861 861
Size: 3845
AclI AA'CG_TT
Cuts at: 3685 3685
Size: 3845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1670 2102 2849 3117 514
Size: 1156 432 747 268 1242
Fragments arranged by size:
1242 1156 747 432 268
AflII C'TTAA_G
Cuts at: 565 565
Size: 3845
AflIII A'CryG_T
Cuts at: 1197 2301 3278 1197
Size: 1104 977 1764
Fragments arranged by size:
1764 1104 977
AgeI A'CCGG_T
Cuts at: 2233 2233
Size: 3845
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 677 709 677
Size: 32 3813
AlwNI CAG_nnn'CTG
Cuts at: 272 1150 2717 272
Size: 878 1567 1400
Fragments arranged by size:
1567 1400 878
ApaLI G'TGCA_C
Cuts at: 2615 2615
Size: 3845
ApoI r'AATT_y
Cuts at: 178 246 713 849 3198 3454 178
Size: 68 467 136 2349 256 569
Fragments arranged by size:
2349 569 467 256 136 68
AseI AT'TA_AT
Cuts at: 20 20
Size: 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
AvaI C'yCGr_G
Cuts at: 865 865
Size: 3845
BamHI G'GATC_C
Cuts at: 870 870
Size: 3845
BanI G'GyrC_C
Cuts at: 861 1275 1523 1558 861
Size: 414 248 35 3148
Fragments arranged by size:
3148 414 248 35
BanII G_rGCy'C
Cuts at: 743 859 1889 743
Size: 116 1030 2699
Fragments arranged by size:
2699 1030 116
BbeI G_GCGC'C
Cuts at: 1279 1527 1279
Size: 248 3597
BbsI GAAGACnn'nnnn_
Cuts at: 212 242 212
Size: 30 3815
BciVI GTATCCnnnnn_n'
Cuts at: 1743 2504 3845 1743
Size: 761 1341 1743
Fragments arranged by size:
1743 1341 761
BclI T'GATC_A
Cuts at: 467 1365 467
Size: 898 2947
BglII A'GATC_T
Cuts at: 1360 3397 1360
Size: 2037 1808
BlpI GC'TnA_GC
Cuts at: 957 957
Size: 3845
Bme1580I G_kGCm'C
Cuts at: 1470 1563 2619 1470
Size: 93 1056 2696
Fragments arranged by size:
2696 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 832 1057 1138 1473 3617 832
Size: 225 81 335 2144 1060
Fragments arranged by size:
2144 1060 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 3845
Bpu10I CC'TnA_GC
Cuts at: 617 617
Size: 3845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1900 2392 2690 1021
Size: 879 492 298 2176
Fragments arranged by size:
2176 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3549 3549
Size: 3845
BsaAI yAC'GTr
Cuts at: 1198 1198
Size: 3845
BsaBI GATnn'nnATC
Cuts at: 6 1359 3396 6
Size: 1353 2037 455
Fragments arranged by size:
2037 1353 455
BsaHI Gr'CG_yC
Cuts at: 1276 1524 2226 3722 1276
Size: 248 702 1496 1399
Fragments arranged by size:
1496 1399 702 248
BsaWI w'CCGG_w
Cuts at: 1034 1555 2233 2507 2654 3065 1034
Size: 521 678 274 147 411 1814
Fragments arranged by size:
1814 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 677 707 677
Size: 30 3815
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 751 751
Size: 3845
BseYI C'CCAG_C
Cuts at: 1270 2028 2605 3101 1270
Size: 758 577 496 2014
Fragments arranged by size:
2014 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 712
Size: 3845
BsiEI CG_ry'CG
Cuts at: 104 1387 1433 2641 104
Size: 1283 46 1208 1308
Fragments arranged by size:
1308 1283 1208 46
BsiHKAI G_wGCw'C
Cuts at: 743 859 1637 1827 2619 743
Size: 116 778 190 792 1969
Fragments arranged by size:
1969 792 778 190 116
Bsp1286I G_dGCh'C
Cuts at: 743 859 1470 1563 1637 1827 1889 2619 743
Size: 116 611 93 74 190 62 730 1969
Fragments arranged by size:
1969 730 611 190 116 93 74 62
BspEI T'CCGG_A
Cuts at: 3065 3065
Size: 3845
BspHI T'CATG_A
Cuts at: 543 3021 543
Size: 2478 1367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 533 895 1109 1411 1792 533
Size: 362 214 302 381 2586
Fragments arranged by size:
2586 381 362 302 214
BsrBI CCG'CTC
Cuts at: 50 2137 2191 3675 3840 50
Size: 2087 54 1484 165 55
Fragments arranged by size:
2087 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1757 1757
Size: 3845
BsrFI r'CCGG_y
Cuts at: 151 1843 2024 2233 151
Size: 1692 181 209 1763
Fragments arranged by size:
1763 1692 209 181
BssSI C'ACGA_G
Cuts at: 2116 2474 2116
Size: 358 3487
BstAPI GCAn_nnn'nTGC
Cuts at: 796 1222 796
Size: 426 3419
BstBI TT'CG_AA
Cuts at: 2206 2206
Size: 3845
BstYI r'GATC_y
Cuts at: 1 870 1337 1360 1695 1941 2953 3039 3051
Size: 869 467 23 335 246 1012 86 12
Cuts at: 3051 3397 1
Size: 346 449
Fragments arranged by size:
1012 869 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1795 1976 2139 1795
Size: 181 163 3501
Fragments arranged by size:
3501 181 163
BtsI GCAGTG_nn'
Cuts at: 1088 1088
Size: 3845
DraI TTT'AAA
Cuts at: 844 844
Size: 3845
DrdI GACnn_nn'nnGTC
Cuts at: 1551 2409 1551
Size: 858 2987
EaeI y'GGCC_r
Cuts at: 1430 1604 1995 2022 3367 1430
Size: 174 391 27 1345 1908
Fragments arranged by size:
1908 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1430 1430
Size: 3845
EarI CTCTTCn'nnn_
Cuts at: 831 1868 2078 831
Size: 1037 210 2598
Fragments arranged by size:
2598 1037 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2053 2363 2509 3655 2053
Size: 310 146 1146 2243
Fragments arranged by size:
2243 1146 310 146
EcoICRI GAG'CTC
Cuts at: 741 857 741
Size: 116 3729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1670 2102 2849 3117 514
Size: 1156 432 747 268 1242
Fragments arranged by size:
1242 1156 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 601 601
Size: 3845
EcoO109I rG'GnC_Cy
Cuts at: 984 984
Size: 3845
EcoRI G'AATT_C
Cuts at: 849 849
Size: 3845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3845
FspI TGC'GCA
Cuts at: 1626 1626
Size: 3845
HaeII r_GCGC'y
Cuts at: 597 1279 1527 2549 3528 597
Size: 682 248 1022 979 914
Fragments arranged by size:
1022 979 914 682 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 315 347 525 557 1687 1717 1719 1749 315
Size: 32 178 32 1130 30 2 30 2411
Fragments arranged by size:
2411 1130 178 32 32 30 30 2
HincII GTy'rAC
Cuts at: 125 309 884 125
Size: 184 575 3086
Fragments arranged by size:
3086 575 184
HpaI GTT'AAC
Cuts at: 309 309
Size: 3845
KasI G'GCGC_C
Cuts at: 1275 1523 1275
Size: 248 3597
KpnI G_GTAC'C
Cuts at: 865 865
Size: 3845
MluI A'CGCG_T
Cuts at: 3278 3278
Size: 3845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 138 813 2516 2700 138
Size: 675 1703 184 1283
Fragments arranged by size:
1703 1283 675 184
MscI TGG'CCA
Cuts at: 1606 1606
Size: 3845
MslI CAynn'nnrTG
Cuts at: 1834 1961 1834
Size: 127 3718
MspA1I CmG'CkG
Cuts at: 956 1270 1630 2643 2888 956
Size: 314 360 1013 245 1913
Fragments arranged by size:
1913 1013 360 314 245
NaeI GCC'GGC
Cuts at: 153 2026 153
Size: 1873 1972
NarI GG'CG_CC
Cuts at: 1276 1524 1276
Size: 248 3597
NgoMIV G'CCGG_C
Cuts at: 151 2024 151
Size: 1873 1972
NspI r_CATG'y
Cuts at: 898 1398 2305 898
Size: 500 907 2438
Fragments arranged by size:
2438 907 500
PciI A'CATG_T
Cuts at: 2301 2301
Size: 3845
PflMI CCAn_nnn'nTGG
Cuts at: 822 822
Size: 3845
PmeI GTTT'AAAC
Cuts at: 844 844
Size: 3845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 677 709 2984 3016 677
Size: 32 2275 32 1506
Fragments arranged by size:
2275 1506 32 32
PstI C_TGCA'G
Cuts at: 892 1577 892
Size: 685 3160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3845
PvuII CAG'CTG
Cuts at: 1270 1630 1270
Size: 360 3485
RsrII CG'GwC_CG
Cuts at: 2040 2040
Size: 3845
SacI G_AGCT'C
Cuts at: 743 859 743
Size: 116 3729
SalI G'TCGA_C
Cuts at: 882 882
Size: 3845
SapI GCTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
SbfI CC_TGCA'GG
Cuts at: 892 892
Size: 3845
ScaI AGT'ACT
Cuts at: 3362 3362
Size: 3845
SexAI A'CCwGG_T
Cuts at: 554 554
Size: 3845
SfcI C'TryA_G
Cuts at: 33 751 888 1573 2566 2757 33
Size: 718 137 685 993 191 1121
Fragments arranged by size:
1121 993 718 685 191 137
SfoI GGC'GCC
Cuts at: 1277 1525 1277
Size: 248 3597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
SmaI CCC'GGG
Cuts at: 867 867
Size: 3845
SmlI C'TyrA_G
Cuts at: 565 1000 1915 2407 2669 565
Size: 435 915 492 262 1741
Fragments arranged by size:
1741 915 492 435 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3845
SphI G_CATG'C
Cuts at: 898 1398 898
Size: 500 3345
StyI C'CwwG_G
Cuts at: 979 1333 979
Size: 354 3491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1428 1561 2020 2229 1428
Size: 133 459 209 3044
Fragments arranged by size:
3044 459 209 133
TatI w'GTAC_w
Cuts at: 3360 3360
Size: 3845
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 3813
Tth111I GACn'n_nGTC
Cuts at: 1642 1642
Size: 3845
XbaI T'CTAG_A
Cuts at: 876 876
Size: 3845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1340 1340
Size: 3845
XmaI C'CCGG_G
Cuts at: 865 865
Size: 3845
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI