Restriction map for
pF1KB8244
(Circular) (Six-base) MAPSORT of: pF1KB8244.seq Check: 3918 from: 1 to: 4115
pF1KB8244 4115 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1153 1153
Size: 4115
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1940 2372 3119 3387 316
Size: 1624 432 747 268 1044
Fragments arranged by size:
1624 1044 747 432 268
AflIII A'CryG_T
Cuts at: 1467 2571 3548 1467
Size: 1104 977 2034
Fragments arranged by size:
2034 1104 977
AgeI A'CCGG_T
Cuts at: 2503 2503
Size: 4115
AlwNI CAG_nnn'CTG
Cuts at: 1420 2987 1420
Size: 1567 2548
ApaLI G'TGCA_C
Cuts at: 2885 2885
Size: 4115
ApoI r'AATT_y
Cuts at: 1119 3468 3724 1119
Size: 2349 256 1510
Fragments arranged by size:
2349 1510 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 422 1135 422
Size: 713 3402
BamHI G'GATC_C
Cuts at: 1140 1140
Size: 4115
BanI G'GyrC_C
Cuts at: 658 1131 1545 1793 1828 658
Size: 473 414 248 35 2945
Fragments arranged by size:
2945 473 414 248 35
BanII G_rGCy'C
Cuts at: 1129 2159 1129
Size: 1030 3085
BbeI G_GCGC'C
Cuts at: 662 1549 1797 662
Size: 887 248 2980
Fragments arranged by size:
2980 887 248
BbsI GAAGACnn'nnnn_
Cuts at: 1004 1004
Size: 4115
BciVI GTATCCnnnnn_n'
Cuts at: 2013 2774 4115 2013
Size: 761 1341 2013
Fragments arranged by size:
2013 1341 761
BclI T'GATC_A
Cuts at: 377 1635 377
Size: 1258 2857
BfrBI ATG'CAT
Cuts at: 309 309
Size: 4115
BglI GCCn_nnn'nGGC
Cuts at: 112 530 112
Size: 418 3697
BglII A'GATC_T
Cuts at: 1630 3667 1630
Size: 2037 2078
BlpI GC'TnA_GC
Cuts at: 1227 1227
Size: 4115
Bme1580I G_kGCm'C
Cuts at: 415 1740 1833 2889 415
Size: 1325 93 1056 1641
Fragments arranged by size:
1641 1325 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 852 1327 1408 1743 3887 852
Size: 475 81 335 2144 1080
Fragments arranged by size:
2144 1080 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4115
Bpu10I CC'TnA_GC
Cuts at: 434 434
Size: 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1291 2170 2662 2960 841
Size: 450 879 492 298 1996
Fragments arranged by size:
1996 879 492 450 298
BsaI GGTCTCn'nnnn_
Cuts at: 3819 3819
Size: 4115
BsaAI yAC'GTr
Cuts at: 1468 1468
Size: 4115
BsaBI GATnn'nnATC
Cuts at: 6 1629 3666 6
Size: 1623 2037 455
Fragments arranged by size:
2037 1623 455
BsaHI Gr'CG_yC
Cuts at: 115 659 1546 1794 2496 3992 115
Size: 544 887 248 702 1496 238
Fragments arranged by size:
1496 887 702 544 248 238
BsaWI w'CCGG_w
Cuts at: 1304 1825 2503 2777 2924 3335 1304
Size: 521 678 274 147 411 2084
Fragments arranged by size:
2084 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 941 945 1540 2298 2875 3371 941
Size: 4 595 758 577 496 1685
Fragments arranged by size:
1685 758 595 577 496 4
BsiEI CG_ry'CG
Cuts at: 104 1657 1703 2911 104
Size: 1553 46 1208 1308
Fragments arranged by size:
1553 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1129 1907 2097 2889 1129
Size: 778 190 792 2355
Fragments arranged by size:
2355 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 415 1129 1740 1833 1907 2097 2159 2889 415
Size: 714 611 93 74 190 62 730 1641
Fragments arranged by size:
1641 730 714 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3335 3335
Size: 4115
BspHI T'CATG_A
Cuts at: 3291 3291
Size: 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1165 1379 1681 2062 1165
Size: 214 302 381 3218
Fragments arranged by size:
3218 381 302 214
BsrBI CCG'CTC
Cuts at: 50 125 2407 2461 3945 4110 50
Size: 75 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 75 55 54
BsrDI GCAATG_nn'
Cuts at: 2027 2027
Size: 4115
BsrFI r'CCGG_y
Cuts at: 2113 2294 2503 2113
Size: 181 209 3725
Fragments arranged by size:
3725 209 181
BsrGI T'GTAC_A
Cuts at: 753 753
Size: 4115
BssSI C'ACGA_G
Cuts at: 2386 2744 2386
Size: 358 3757
BstAPI GCAn_nnn'nTGC
Cuts at: 1492 1492
Size: 4115
BstBI TT'CG_AA
Cuts at: 2476 2476
Size: 4115
BstYI r'GATC_y
Cuts at: 1 1140 1607 1630 1965 2211 3223 3309 3321
Size: 1139 467 23 335 246 1012 86 12
Cuts at: 3321 3667 1
Size: 346 449
Fragments arranged by size:
1139 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1053 107
Size: 946 3169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2065 2246 2409 2065
Size: 181 163 3771
Fragments arranged by size:
3771 181 163
BtsI GCAGTG_nn'
Cuts at: 614 756 1358 614
Size: 142 602 3371
Fragments arranged by size:
3371 602 142
ClaI AT'CG_AT
Cuts at: 182 182
Size: 4115
DraI TTT'AAA
Cuts at: 1114 1114
Size: 4115
DrdI GACnn_nn'nnGTC
Cuts at: 1821 2679 1821
Size: 858 3257
EaeI y'GGCC_r
Cuts at: 152 489 1700 1874 2265 2292 3637 152
Size: 337 1211 174 391 27 1345 630
Fragments arranged by size:
1345 1211 630 391 337 174 27
EagI C'GGCC_G
Cuts at: 1700 1700
Size: 4115
EarI CTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 2633 2779 3925 2323
Size: 310 146 1146 2513
Fragments arranged by size:
2513 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1127 1127
Size: 4115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1940 2372 3119 3387 316
Size: 1624 432 747 268 1044
Fragments arranged by size:
1624 1044 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 698 698
Size: 4115
EcoO109I rG'GnC_Cy
Cuts at: 703 1254 703
Size: 551 3564
EcoRI G'AATT_C
Cuts at: 1119 1119
Size: 4115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 670 1021 2134 670
Size: 351 1113 2651
Fragments arranged by size:
2651 1113 351
FspI TGC'GCA
Cuts at: 1896 1896
Size: 4115
HaeII r_GCGC'y
Cuts at: 662 1549 1797 2819 3798 662
Size: 887 248 1022 979 979
Fragments arranged by size:
1022 979 979 887 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 234 266 564 596 976 1008 1957 1987 1989
Size: 32 298 32 380 32 949 30 2
Cuts at: 1989 2019 234
Size: 30 2330
Fragments arranged by size:
2330 949 380 298 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1154 1154
Size: 4115
KasI G'GCGC_C
Cuts at: 658 1545 1793 658
Size: 887 248 2980
Fragments arranged by size:
2980 887 248
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 646 2786 2970 646
Size: 2140 184 1791
Fragments arranged by size:
2140 1791 184
MscI TGG'CCA
Cuts at: 154 1876 154
Size: 1722 2393
MslI CAynn'nnrTG
Cuts at: 2104 2231 2104
Size: 127 3988
MspA1I CmG'CkG
Cuts at: 945 1028 1226 1540 1900 2913 3158 945
Size: 83 198 314 360 1013 245 1902
Fragments arranged by size:
1902 1013 360 314 245 198 83
NaeI GCC'GGC
Cuts at: 2296 2296
Size: 4115
NarI GG'CG_CC
Cuts at: 659 1546 1794 659
Size: 887 248 2980
Fragments arranged by size:
2980 887 248
NcoI C'CATG_G
Cuts at: 107 1053 107
Size: 946 3169
NdeI CA'TA_TG
Cuts at: 447 447
Size: 4115
NgoMIV G'CCGG_C
Cuts at: 2294 2294
Size: 4115
NsiI A_TGCA'T
Cuts at: 311 311
Size: 4115
NspI r_CATG'y
Cuts at: 165 313 1168 1668 2575 165
Size: 148 855 500 907 1705
Fragments arranged by size:
1705 907 855 500 148
PciI A'CATG_T
Cuts at: 2571 2571
Size: 4115
PfoI T'CCnGG_A
Cuts at: 118 590 118
Size: 472 3643
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 4083
PpuMI rG'GwC_Cy
Cuts at: 703 703
Size: 4115
PstI C_TGCA'G
Cuts at: 1162 1847 1162
Size: 685 3430
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4115
PvuII CAG'CTG
Cuts at: 945 1028 1540 1900 945
Size: 83 512 360 3160
Fragments arranged by size:
3160 512 360 83
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 1129 1129
Size: 4115
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SapI GCTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
SbfI CC_TGCA'GG
Cuts at: 1162 1162
Size: 4115
ScaI AGT'ACT
Cuts at: 268 567 3632 268
Size: 299 3065 751
Fragments arranged by size:
3065 751 299
SfcI C'TryA_G
Cuts at: 33 1158 1843 2836 3027 33
Size: 1125 685 993 191 1121
Fragments arranged by size:
1125 1121 993 685 191
SfoI GGC'GCC
Cuts at: 660 1547 1795 660
Size: 887 248 2980
Fragments arranged by size:
2980 887 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 1137 1137
Size: 4115
SmlI C'TyrA_G
Cuts at: 820 1270 2185 2677 2939 820
Size: 450 915 492 262 1996
Fragments arranged by size:
1996 915 492 450 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 313 1168 1668 313
Size: 855 500 2760
Fragments arranged by size:
2760 855 500
SspI AAT'ATT
Cuts at: 769 769
Size: 4115
StyI C'CwwG_G
Cuts at: 107 1053 1249 1603 107
Size: 946 196 354 2619
Fragments arranged by size:
2619 946 354 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 834 1001 1698 1831 2290 2499 834
Size: 167 697 133 459 209 2450
Fragments arranged by size:
2450 697 459 209 167 133
TatI w'GTAC_w
Cuts at: 266 565 753 3630 266
Size: 299 188 2877 751
Fragments arranged by size:
2877 751 299 188
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4083
Tth111I GACn'n_nGTC
Cuts at: 1912 1912
Size: 4115
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 532 1610 381
Size: 151 1078 2886
Fragments arranged by size:
2886 1078 151
XmaI C'CCGG_G
Cuts at: 1135 1135
Size: 4115
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI