Restriction map for
pF1KB8245
(Circular) (Six-base) MAPSORT of: pF1KB8245.seq Check: 8085 from: 1 to: 3653
pF1KB8245 3653 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 691 691
Size: 3653
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 526 1478 1910 2657 2925 238
Size: 288 952 432 747 268 966
Fragments arranged by size:
966 952 747 432 288 268
AflIII A'CryG_T
Cuts at: 645 1005 2109 3086 645
Size: 360 1104 977 1212
Fragments arranged by size:
1212 1104 977 360
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AhdI GACnn_n'nnGTC
Cuts at: 624 624
Size: 3653
AlwNI CAG_nnn'CTG
Cuts at: 958 2525 958
Size: 1567 2086
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 404 657 3006 3262 404
Size: 253 2349 256 795
Fragments arranged by size:
2349 795 256 253
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 673 673
Size: 3653
BamHI G'GATC_C
Cuts at: 678 678
Size: 3653
BanI G'GyrC_C
Cuts at: 669 1083 1331 1366 669
Size: 414 248 35 2956
Fragments arranged by size:
2956 414 248 35
BanII G_rGCy'C
Cuts at: 667 1697 667
Size: 1030 2623
BbeI G_GCGC'C
Cuts at: 1087 1335 1087
Size: 248 3405
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 765 765
Size: 3653
Bme1580I G_kGCm'C
Cuts at: 1278 1371 2427 1278
Size: 93 1056 2504
Fragments arranged by size:
2504 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 865 946 1281 3425 865
Size: 81 335 2144 1093
Fragments arranged by size:
2144 1093 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 3653
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 1084 1332 2034 3530 1084
Size: 248 702 1496 1207
Fragments arranged by size:
1496 1207 702 248
BsaWI w'CCGG_w
Cuts at: 842 1363 2041 2315 2462 2873 842
Size: 521 678 274 147 411 1622
Fragments arranged by size:
1622 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 114 114
Size: 3653
BseYI C'CCAG_C
Cuts at: 1078 1836 2413 2909 1078
Size: 758 577 496 1822
Fragments arranged by size:
1822 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 396 1195 1241 2449 104
Size: 292 799 46 1208 1308
Fragments arranged by size:
1308 1208 799 292 46
BsiHKAI G_wGCw'C
Cuts at: 667 1445 1635 2427 667
Size: 778 190 792 1893
Fragments arranged by size:
1893 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 667 1278 1371 1445 1635 1697 2427 667
Size: 611 93 74 190 62 730 1893
Fragments arranged by size:
1893 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2873 2873
Size: 3653
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 917 1219 1600 703
Size: 214 302 381 2756
Fragments arranged by size:
2756 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1945 1999 3483 3648 50
Size: 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1565 1565
Size: 3653
BsrFI r'CCGG_y
Cuts at: 1651 1832 2041 1651
Size: 181 209 3263
Fragments arranged by size:
3263 209 181
BssSI C'ACGA_G
Cuts at: 1924 2282 1924
Size: 358 3295
BstAPI GCAn_nnn'nTGC
Cuts at: 435 1030 435
Size: 595 3058
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstYI r'GATC_y
Cuts at: 1 678 1145 1168 1503 1749 2761 2847 2859
Size: 677 467 23 335 246 1012 86 12
Cuts at: 2859 3205 1
Size: 346 449
Fragments arranged by size:
1012 677 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1603 1784 1947 1603
Size: 181 163 3309
Fragments arranged by size:
3309 181 163
BtsI GCAGTG_nn'
Cuts at: 470 896 470
Size: 426 3227
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 509 1238 1412 1803 1830 3175 509
Size: 729 174 391 27 1345 987
Fragments arranged by size:
1345 987 729 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 136 554 1676 1886 136
Size: 418 1122 210 1903
Fragments arranged by size:
1903 1122 418 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1861 2171 2317 3463 1861
Size: 310 146 1146 2051
Fragments arranged by size:
2051 1146 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 526 1478 1910 2657 2925 238
Size: 288 952 432 747 268 966
Fragments arranged by size:
966 952 747 432 288 268
EcoO109I rG'GnC_Cy
Cuts at: 792 792
Size: 3653
EcoRI G'AATT_C
Cuts at: 404 657 404
Size: 253 3400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 366 1672 366
Size: 1306 2347
FspI TGC'GCA
Cuts at: 163 1434 163
Size: 1271 2382
FspAI rTGC'GCAy
Cuts at: 163 163
Size: 3653
HaeII r_GCGC'y
Cuts at: 1087 1335 2357 3336 1087
Size: 248 1022 979 1404
Fragments arranged by size:
1404 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 610 642 1495 1525 1527 1557 610
Size: 32 853 30 2 30 2706
Fragments arranged by size:
2706 853 32 30 30 2
HincII GTy'rAC
Cuts at: 692 692
Size: 3653
KasI G'GCGC_C
Cuts at: 1083 1331 1083
Size: 248 3405
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 156 2324 2508 156
Size: 2168 184 1301
Fragments arranged by size:
2168 1301 184
MscI TGG'CCA
Cuts at: 511 1414 511
Size: 903 2750
MslI CAynn'nnrTG
Cuts at: 169 1642 1769 169
Size: 1473 127 2053
Fragments arranged by size:
2053 1473 127
MspA1I CmG'CkG
Cuts at: 764 1078 1438 2451 2696 764
Size: 314 360 1013 245 1721
Fragments arranged by size:
1721 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 1084 1332 1084
Size: 248 3405
NcoI C'CATG_G
Cuts at: 107 107
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PasI CC'CwG_GG
Cuts at: 363 363
Size: 3653
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PstI C_TGCA'G
Cuts at: 431 700 1385 431
Size: 269 685 2699
Fragments arranged by size:
2699 685 269
PvuI CG_AT'CG
Cuts at: 104 396 104
Size: 292 3361
PvuII CAG'CTG
Cuts at: 1078 1438 1078
Size: 360 3293
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 427 696 1381 2374 2565 33
Size: 394 269 685 993 191 1121
Fragments arranged by size:
1121 993 685 394 269 191
SfoI GGC'GCC
Cuts at: 1085 1333 1085
Size: 248 3405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 675 675
Size: 3653
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StuI AGG'CCT
Cuts at: 333 333
Size: 3653
StyI C'CwwG_G
Cuts at: 107 787 1141 107
Size: 680 354 2619
Fragments arranged by size:
2619 680 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1236 1369 1828 2037 1236
Size: 133 459 209 2852
Fragments arranged by size:
2852 459 209 133
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 228 260 3170 3202 228
Size: 32 2910 32 679
Fragments arranged by size:
2910 679 32 32
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1148 1148
Size: 3653
XmaI C'CCGG_G
Cuts at: 673 673
Size: 3653
XmnI GAAnn'nnTTC
Cuts at: 404 404
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI