Restriction map for
pF1KB8249
(Circular) (Six-base) MAPSORT of: pF1KB8249.seq Check: 2991 from: 1 to: 3830
pF1KB8249 3830 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 498 675 498
Size: 177 3653
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 139 1655 2087 2834 3102 138
Size: 1 1516 432 747 268 866
Fragments arranged by size:
1516 866 747 432 268 1
AflIII A'CryG_T
Cuts at: 1182 2286 3263 1182
Size: 1104 977 1749
Fragments arranged by size:
1749 1104 977
AgeI A'CCGG_T
Cuts at: 2218 2218
Size: 3830
AlwNI CAG_nnn'CTG
Cuts at: 616 1135 2702 616
Size: 519 1567 1744
Fragments arranged by size:
1744 1567 519
ApaLI G'TGCA_C
Cuts at: 697 2600 697
Size: 1903 1927
ApoI r'AATT_y
Cuts at: 480 834 3183 3439 480
Size: 354 2349 256 871
Fragments arranged by size:
2349 871 354 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 850 850
Size: 3830
BamHI G'GATC_C
Cuts at: 855 855
Size: 3830
BanI G'GyrC_C
Cuts at: 846 1260 1508 1543 846
Size: 414 248 35 3133
Fragments arranged by size:
3133 414 248 35
BanII G_rGCy'C
Cuts at: 755 844 1874 755
Size: 89 1030 2711
Fragments arranged by size:
2711 1030 89
BbeI G_GCGC'C
Cuts at: 1264 1512 1264
Size: 248 3582
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2489 3830 1728
Size: 761 1341 1728
Fragments arranged by size:
1728 1341 761
BclI T'GATC_A
Cuts at: 512 1350 512
Size: 838 2992
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3830
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 942 942
Size: 3830
Bme1580I G_kGCm'C
Cuts at: 701 1455 1548 2604 701
Size: 754 93 1056 1927
Fragments arranged by size:
1927 1056 754 93
BmrI ACTGGGnnnn_n'
Cuts at: 1042 1123 1458 3602 1042
Size: 81 335 2144 1270
Fragments arranged by size:
2144 1270 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1885 2377 2675 1006
Size: 879 492 298 2161
Fragments arranged by size:
2161 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3534 3534
Size: 3830
BsaAI yAC'GTr
Cuts at: 1183 1183
Size: 3830
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 1261 1509 2211 3707 1261
Size: 248 702 1496 1384
Fragments arranged by size:
1496 1384 702 248
BsaWI w'CCGG_w
Cuts at: 1019 1540 2218 2492 2639 3050 1019
Size: 521 678 274 147 411 1799
Fragments arranged by size:
1799 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 691 721 691
Size: 30 3800
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 345 502 796 345
Size: 157 294 3379
Fragments arranged by size:
3379 294 157
BseYI C'CCAG_C
Cuts at: 1255 2013 2590 3086 1255
Size: 758 577 496 1999
Fragments arranged by size:
1999 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1372 1418 2626 104
Size: 1268 46 1208 1308
Fragments arranged by size:
1308 1268 1208 46
BsiHKAI G_wGCw'C
Cuts at: 701 755 844 1622 1812 2604 701
Size: 54 89 778 190 792 1927
Fragments arranged by size:
1927 792 778 190 89 54
Bsp1286I G_dGCh'C
Cuts at: 701 755 844 1455 1548 1622 1812 1874 2604
Size: 54 89 611 93 74 190 62 730
Cuts at: 2604 701
Size: 1927
Fragments arranged by size:
1927 730 611 190 93 89 74 62
54
BspEI T'CCGG_A
Cuts at: 3050 3050
Size: 3830
BspHI T'CATG_A
Cuts at: 3006 3006
Size: 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 498 675 821 880 1094 1396 1777 498
Size: 177 146 59 214 302 381 2551
Fragments arranged by size:
2551 381 302 214 177 146 59
BsrBI CCG'CTC
Cuts at: 50 786 2122 2176 3660 3825 50
Size: 736 1336 54 1484 165 55
Fragments arranged by size:
1484 1336 736 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1742 1742
Size: 3830
BsrFI r'CCGG_y
Cuts at: 1828 2009 2218 1828
Size: 181 209 3440
Fragments arranged by size:
3440 209 181
BssSI C'ACGA_G
Cuts at: 2101 2459 2101
Size: 358 3472
BstAPI GCAn_nnn'nTGC
Cuts at: 616 1207 616
Size: 591 3239
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstYI r'GATC_y
Cuts at: 1 855 1322 1345 1680 1926 2938 3024 3036
Size: 854 467 23 335 246 1012 86 12
Cuts at: 3036 3382 1
Size: 346 449
Fragments arranged by size:
1012 854 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 229 107
Size: 122 3708
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1780 1961 2124 1780
Size: 181 163 3486
Fragments arranged by size:
3486 181 163
BtsI GCAGTG_nn'
Cuts at: 344 1073 344
Size: 729 3101
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2394 1536
Size: 858 2972
EaeI y'GGCC_r
Cuts at: 1415 1589 1980 2007 3352 1415
Size: 174 391 27 1345 1893
Fragments arranged by size:
1893 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1415 1415
Size: 3830
EarI CTCTTCn'nnn_
Cuts at: 115 686 743 1853 2063 115
Size: 571 57 1110 210 1882
Fragments arranged by size:
1882 1110 571 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2038 2348 2494 3640 2038
Size: 310 146 1146 2228
Fragments arranged by size:
2228 1146 310 146
EcoICRI GAG'CTC
Cuts at: 753 842 753
Size: 89 3741
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 139 1655 2087 2834 3102 138
Size: 1 1516 432 747 268 866
Fragments arranged by size:
1516 866 747 432 268 1
EcoNI CCTnn'n_nnAGG
Cuts at: 817 817
Size: 3830
EcoO109I rG'GnC_Cy
Cuts at: 969 969
Size: 3830
EcoRI G'AATT_C
Cuts at: 480 834 480
Size: 354 3476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3830
FspI TGC'GCA
Cuts at: 1611 1611
Size: 3830
HaeII r_GCGC'y
Cuts at: 1264 1512 2534 3513 1264
Size: 248 1022 979 1581
Fragments arranged by size:
1581 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 628 660 1672 1702 1704 1734 346
Size: 32 250 32 1012 30 2 30 2442
Fragments arranged by size:
2442 1012 250 32 32 30 30 2
HincII GTy'rAC
Cuts at: 869 869
Size: 3830
KasI G'GCGC_C
Cuts at: 1260 1508 1260
Size: 248 3582
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 776 2501 2685 776
Size: 1725 184 1921
Fragments arranged by size:
1921 1725 184
MscI TGG'CCA
Cuts at: 1591 1591
Size: 3830
MslI CAynn'nnrTG
Cuts at: 577 1819 1946 577
Size: 1242 127 2461
Fragments arranged by size:
2461 1242 127
MspA1I CmG'CkG
Cuts at: 672 941 1255 1615 2628 2873 672
Size: 269 314 360 1013 245 1629
Fragments arranged by size:
1629 1013 360 314 269 245
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 3830
NarI GG'CG_CC
Cuts at: 1261 1509 1261
Size: 248 3582
NcoI C'CATG_G
Cuts at: 107 229 107
Size: 122 3708
NdeI CA'TA_TG
Cuts at: 387 387
Size: 3830
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 3830
NspI r_CATG'y
Cuts at: 883 1383 2290 883
Size: 500 907 2423
Fragments arranged by size:
2423 907 500
PciI A'CATG_T
Cuts at: 2286 2286
Size: 3830
PfoI T'CCnGG_A
Cuts at: 709 709
Size: 3830
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3798
PstI C_TGCA'G
Cuts at: 588 686 877 1562 588
Size: 98 191 685 2856
Fragments arranged by size:
2856 685 191 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 672 1255 1615 672
Size: 583 360 2887
Fragments arranged by size:
2887 583 360
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 755 844 755
Size: 89 3741
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 743 1853 2063 743
Size: 1110 210 2510
Fragments arranged by size:
2510 1110 210
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 556 3347 556
Size: 2791 1039
SfcI C'TryA_G
Cuts at: 33 584 682 873 1558 2551 2742 33
Size: 551 98 191 685 993 191 1121
Fragments arranged by size:
1121 993 685 551 191 191 98
SfoI GGC'GCC
Cuts at: 1262 1510 1262
Size: 248 3582
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 852 852
Size: 3830
SmlI C'TyrA_G
Cuts at: 985 1900 2392 2654 985
Size: 915 492 262 2161
Fragments arranged by size:
2161 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 883 1383 883
Size: 500 3330
StuI AGG'CCT
Cuts at: 643 808 643
Size: 165 3665
StyI C'CwwG_G
Cuts at: 107 229 964 1318 107
Size: 122 735 354 2619
Fragments arranged by size:
2619 735 354 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 1546 2005 2214 1413
Size: 133 459 209 3029
Fragments arranged by size:
3029 459 209 133
TatI w'GTAC_w
Cuts at: 554 3345 554
Size: 2791 1039
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 691 723 3347 3379 691
Size: 32 2624 32 1142
Fragments arranged by size:
2624 1142 32 32
Tth111I GACn'n_nGTC
Cuts at: 1627 1627
Size: 3830
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1325 1325
Size: 3830
XmaI C'CCGG_G
Cuts at: 850 850
Size: 3830
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI