Restriction map for
pF1KB8251
(Circular) (Six-base) MAPSORT of: pF1KB8251.seq Check: 4038 from: 1 to: 3794
pF1KB8251 3794 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 832 832
Size: 3794
Acc65I G'GTAC_C
Cuts at: 810 810
Size: 3794
AclI AA'CG_TT
Cuts at: 3634 3634
Size: 3794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1619 2051 2798 3066 1619
Size: 432 747 268 2347
Fragments arranged by size:
2347 747 432 268
AflII C'TTAA_G
Cuts at: 697 697
Size: 3794
AflIII A'CryG_T
Cuts at: 1146 2250 3227 1146
Size: 1104 977 1713
Fragments arranged by size:
1713 1104 977
AgeI A'CCGG_T
Cuts at: 2182 2182
Size: 3794
AlwNI CAG_nnn'CTG
Cuts at: 1099 2666 1099
Size: 1567 2227
ApaLI G'TGCA_C
Cuts at: 2564 2564
Size: 3794
ApoI r'AATT_y
Cuts at: 127 391 619 798 3147 3403 127
Size: 264 228 179 2349 256 518
Fragments arranged by size:
2349 518 264 256 228 179
AseI AT'TA_AT
Cuts at: 20 20
Size: 3794
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
AvaI C'yCGr_G
Cuts at: 814 814
Size: 3794
BamHI G'GATC_C
Cuts at: 819 819
Size: 3794
BanI G'GyrC_C
Cuts at: 810 1224 1472 1507 810
Size: 414 248 35 3097
Fragments arranged by size:
3097 414 248 35
BanII G_rGCy'C
Cuts at: 808 1838 808
Size: 1030 2764
BbeI G_GCGC'C
Cuts at: 1228 1476 1228
Size: 248 3546
BciVI GTATCCnnnnn_n'
Cuts at: 1692 2453 3794 1692
Size: 761 1341 1692
Fragments arranged by size:
1692 1341 761
BclI T'GATC_A
Cuts at: 1314 1314
Size: 3794
BglII A'GATC_T
Cuts at: 1309 3346 1309
Size: 2037 1757
BlpI GC'TnA_GC
Cuts at: 906 906
Size: 3794
Bme1580I G_kGCm'C
Cuts at: 236 1419 1512 2568 236
Size: 1183 93 1056 1462
Fragments arranged by size:
1462 1183 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1006 1087 1422 3566 1006
Size: 81 335 2144 1234
Fragments arranged by size:
2144 1234 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 3794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1849 2341 2639 970
Size: 879 492 298 2125
Fragments arranged by size:
2125 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3498 3498
Size: 3794
BsaAI yAC'GTr
Cuts at: 1147 1147
Size: 3794
BsaBI GATnn'nnATC
Cuts at: 6 1308 3345 6
Size: 1302 2037 455
Fragments arranged by size:
2037 1302 455
BsaHI Gr'CG_yC
Cuts at: 1225 1473 2175 3671 1225
Size: 248 702 1496 1348
Fragments arranged by size:
1496 1348 702 248
BsaWI w'CCGG_w
Cuts at: 983 1504 2182 2456 2603 3014 983
Size: 521 678 274 147 411 1763
Fragments arranged by size:
1763 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 342 372 342
Size: 30 3764
BseYI C'CCAG_C
Cuts at: 492 1219 1977 2554 3050 492
Size: 727 758 577 496 1236
Fragments arranged by size:
1236 758 727 577 496
BsiEI CG_ry'CG
Cuts at: 104 1336 1382 2590 104
Size: 1232 46 1208 1308
Fragments arranged by size:
1308 1232 1208 46
BsiHKAI G_wGCw'C
Cuts at: 808 1586 1776 2568 808
Size: 778 190 792 2034
Fragments arranged by size:
2034 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 236 808 1419 1512 1586 1776 1838 2568 236
Size: 572 611 93 74 190 62 730 1462
Fragments arranged by size:
1462 730 611 572 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3014 3014
Size: 3794
BspHI T'CATG_A
Cuts at: 2970 2970
Size: 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1058 1360 1741 844
Size: 214 302 381 2897
Fragments arranged by size:
2897 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2086 2140 3624 3789 50
Size: 2036 54 1484 165 55
Fragments arranged by size:
2036 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 494 560 1706 494
Size: 66 1146 2582
Fragments arranged by size:
2582 1146 66
BsrFI r'CCGG_y
Cuts at: 1792 1973 2182 1792
Size: 181 209 3404
Fragments arranged by size:
3404 209 181
BssSI C'ACGA_G
Cuts at: 2065 2423 2065
Size: 358 3436
BstAPI GCAn_nnn'nTGC
Cuts at: 1171 1171
Size: 3794
BstBI TT'CG_AA
Cuts at: 395 2155 395
Size: 1760 2034
BstYI r'GATC_y
Cuts at: 1 819 1286 1309 1644 1890 2902 2988 3000
Size: 818 467 23 335 246 1012 86 12
Cuts at: 3000 3346 1
Size: 346 449
Fragments arranged by size:
1012 818 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1744 1925 2088 1744
Size: 181 163 3450
Fragments arranged by size:
3450 181 163
BtsI GCAGTG_nn'
Cuts at: 1037 1037
Size: 3794
DraI TTT'AAA
Cuts at: 312 740 793 312
Size: 428 53 3313
Fragments arranged by size:
3313 428 53
DrdI GACnn_nn'nnGTC
Cuts at: 1500 2358 1500
Size: 858 2936
EaeI y'GGCC_r
Cuts at: 122 1379 1553 1944 1971 3316 122
Size: 1257 174 391 27 1345 600
Fragments arranged by size:
1345 1257 600 391 174 27
EagI C'GGCC_G
Cuts at: 1379 1379
Size: 3794
EarI CTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2002 2312 2458 3604 2002
Size: 310 146 1146 2192
Fragments arranged by size:
2192 1146 310 146
EcoICRI GAG'CTC
Cuts at: 806 806
Size: 3794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1619 2051 2798 3066 1619
Size: 432 747 268 2347
Fragments arranged by size:
2347 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 933 933
Size: 3794
EcoRI G'AATT_C
Cuts at: 391 798 391
Size: 407 3387
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1813 1813
Size: 3794
FspI TGC'GCA
Cuts at: 1575 1575
Size: 3794
HaeII r_GCGC'y
Cuts at: 1228 1476 2498 3477 1228
Size: 248 1022 979 1545
Fragments arranged by size:
1545 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 564 596 1636 1666 1668 1698 268
Size: 32 264 32 1040 30 2 30 2364
Fragments arranged by size:
2364 1040 264 32 32 30 30 2
HincII GTy'rAC
Cuts at: 833 833
Size: 3794
HindIII A'AGCT_T
Cuts at: 678 678
Size: 3794
KasI G'GCGC_C
Cuts at: 1224 1472 1224
Size: 248 3546
KpnI G_GTAC'C
Cuts at: 814 814
Size: 3794
MluI A'CGCG_T
Cuts at: 3227 3227
Size: 3794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2649 2465
Size: 184 3610
MscI TGG'CCA
Cuts at: 1555 1555
Size: 3794
MslI CAynn'nnrTG
Cuts at: 1783 1910 1783
Size: 127 3667
MspA1I CmG'CkG
Cuts at: 905 1219 1579 2592 2837 905
Size: 314 360 1013 245 1862
Fragments arranged by size:
1862 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1975 1975
Size: 3794
NarI GG'CG_CC
Cuts at: 1225 1473 1225
Size: 248 3546
NdeI CA'TA_TG
Cuts at: 291 363 291
Size: 72 3722
NgoMIV G'CCGG_C
Cuts at: 1973 1973
Size: 3794
NspI r_CATG'y
Cuts at: 847 1347 2254 847
Size: 500 907 2387
Fragments arranged by size:
2387 907 500
PciI A'CATG_T
Cuts at: 2250 2250
Size: 3794
PmeI GTTT'AAAC
Cuts at: 793 793
Size: 3794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2933 2965 2933
Size: 32 3762
PstI C_TGCA'G
Cuts at: 841 1526 841
Size: 685 3109
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3794
PvuII CAG'CTG
Cuts at: 1219 1579 1219
Size: 360 3434
RsrII CG'GwC_CG
Cuts at: 1989 1989
Size: 3794
SacI G_AGCT'C
Cuts at: 808 808
Size: 3794
SalI G'TCGA_C
Cuts at: 831 831
Size: 3794
SapI GCTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
SbfI CC_TGCA'GG
Cuts at: 841 841
Size: 3794
ScaI AGT'ACT
Cuts at: 3311 3311
Size: 3794
SfcI C'TryA_G
Cuts at: 33 837 1522 2515 2706 33
Size: 804 685 993 191 1121
Fragments arranged by size:
1121 993 804 685 191
SfoI GGC'GCC
Cuts at: 1226 1474 1226
Size: 248 3546
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
SmaI CCC'GGG
Cuts at: 816 816
Size: 3794
SmlI C'TyrA_G
Cuts at: 697 949 1864 2356 2618 697
Size: 252 915 492 262 1873
Fragments arranged by size:
1873 915 492 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3794
SphI G_CATG'C
Cuts at: 847 1347 847
Size: 500 3294
SspI AAT'ATT
Cuts at: 413 413
Size: 3794
StyI C'CwwG_G
Cuts at: 928 1282 928
Size: 354 3440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1377 1510 1969 2178 1377
Size: 133 459 209 2993
Fragments arranged by size:
2993 459 209 133
TatI w'GTAC_w
Cuts at: 3309 3309
Size: 3794
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3311 3343 3311
Size: 32 3762
Tth111I GACn'n_nGTC
Cuts at: 1591 1591
Size: 3794
XbaI T'CTAG_A
Cuts at: 825 825
Size: 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 1289 489
Size: 800 2994
XmaI C'CCGG_G
Cuts at: 814 814
Size: 3794
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI